Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_04735 and IAI47_04740 overlap by 14 nucleotides IAI47_04740 and IAI47_04745 overlap by 25 nucleotides IAI47_04745 and IAI47_04750 are separated by 13 nucleotides IAI47_04750 and IAI47_04755 overlap by 4 nucleotides IAI47_04755 and IAI47_04760 are separated by 521 nucleotides
IAI47_04735: IAI47_04735 - DUF5375 family protein, at 999,010 to 999,351
_04735
IAI47_04740: IAI47_04740 - hypothetical protein, at 999,338 to 999,604
_04740
IAI47_04745: IAI47_04745 - ash family protein, at 999,580 to 1,000,128
_04745
IAI47_04750: IAI47_04750 - hypothetical protein, at 1,000,142 to 1,000,384
_04750
IAI47_04755: IAI47_04755 - AlpA family transcriptional regulator, at 1,000,381 to 1,000,638
_04755
IAI47_04760: IAI47_04760 - hypothetical protein, at 1,001,160 to 1,001,876
_04760
Position (kb) 1000
1001 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 999.185 kb on + strand, within IAI47_04735 at 999.185 kb on + strand, within IAI47_04735 at 999.185 kb on + strand, within IAI47_04735 at 999.185 kb on + strand, within IAI47_04735 at 999.186 kb on - strand, within IAI47_04735 at 999.186 kb on - strand, within IAI47_04735 at 999.186 kb on - strand, within IAI47_04735 at 999.186 kb on - strand, within IAI47_04735 at 999.186 kb on - strand, within IAI47_04735 at 999.186 kb on - strand, within IAI47_04735 at 999.186 kb on - strand, within IAI47_04735 at 999.186 kb on - strand, within IAI47_04735 at 999.275 kb on + strand, within IAI47_04735 at 999.300 kb on - strand, within IAI47_04735 at 999.313 kb on + strand, within IAI47_04735 at 999.313 kb on + strand, within IAI47_04735 at 999.313 kb on + strand, within IAI47_04735 at 999.314 kb on - strand, within IAI47_04735 at 999.369 kb on + strand, within IAI47_04740 at 999.369 kb on - strand, within IAI47_04740 at 999.543 kb on + strand, within IAI47_04740 at 999.543 kb on + strand, within IAI47_04740 at 999.544 kb on - strand, within IAI47_04740 at 999.544 kb on - strand, within IAI47_04740 at 999.564 kb on + strand, within IAI47_04740 at 999.564 kb on + strand, within IAI47_04740 at 999.564 kb on + strand, within IAI47_04740 at 999.564 kb on + strand, within IAI47_04740 at 999.598 kb on + strand at 999.598 kb on + strand at 999.598 kb on + strand at 999.598 kb on + strand at 999.598 kb on + strand at 999.599 kb on - strand at 999.601 kb on + strand at 999.601 kb on + strand at 999.601 kb on + strand at 999.602 kb on - strand at 999.602 kb on - strand at 999.602 kb on - strand at 999.602 kb on - strand at 999.602 kb on - strand at 999.650 kb on + strand, within IAI47_04745 at 999.650 kb on + strand, within IAI47_04745 at 999.651 kb on - strand, within IAI47_04745 at 999.651 kb on - strand, within IAI47_04745 at 999.651 kb on - strand, within IAI47_04745 at 999.699 kb on + strand, within IAI47_04745 at 999.700 kb on - strand, within IAI47_04745 at 999.708 kb on + strand, within IAI47_04745 at 999.708 kb on + strand, within IAI47_04745 at 999.708 kb on + strand, within IAI47_04745 at 999.708 kb on + strand, within IAI47_04745 at 999.709 kb on - strand, within IAI47_04745 at 999.709 kb on - strand, within IAI47_04745 at 999.709 kb on - strand, within IAI47_04745 at 999.709 kb on - strand, within IAI47_04745 at 999.753 kb on + strand, within IAI47_04745 at 999.753 kb on + strand, within IAI47_04745 at 999.754 kb on - strand, within IAI47_04745 at 999.754 kb on - strand, within IAI47_04745 at 999.780 kb on + strand, within IAI47_04745 at 999.780 kb on + strand, within IAI47_04745 at 999.795 kb on + strand, within IAI47_04745 at 999.795 kb on + strand, within IAI47_04745 at 999.795 kb on + strand, within IAI47_04745 at 999.795 kb on + strand, within IAI47_04745 at 999.795 kb on + strand, within IAI47_04745 at 999.795 kb on + strand, within IAI47_04745 at 999.796 kb on - strand, within IAI47_04745 at 999.814 kb on + strand, within IAI47_04745 at 999.814 kb on + strand, within IAI47_04745 at 999.815 kb on - strand, within IAI47_04745 at 999.820 kb on + strand, within IAI47_04745 at 999.820 kb on + strand, within IAI47_04745 at 999.820 kb on + strand, within IAI47_04745 at 999.820 kb on + strand, within IAI47_04745 at 999.820 kb on + strand, within IAI47_04745 at 999.820 kb on + strand, within IAI47_04745 at 999.820 kb on + strand, within IAI47_04745 at 999.821 kb on - strand, within IAI47_04745 at 999.822 kb on + strand, within IAI47_04745 at 999.823 kb on - strand, within IAI47_04745 at 999.823 kb on - strand, within IAI47_04745 at 999.829 kb on - strand, within IAI47_04745 at 999.829 kb on - strand, within IAI47_04745 at 999.829 kb on - strand, within IAI47_04745 at 999.843 kb on + strand, within IAI47_04745 at 999.844 kb on - strand, within IAI47_04745 at 999.844 kb on - strand at 999.844 kb on - strand, within IAI47_04745 at 999.874 kb on - strand, within IAI47_04745 at 999.935 kb on - strand, within IAI47_04745 at 999.935 kb on - strand, within IAI47_04745 at 1000.036 kb on + strand, within IAI47_04745 at 1000.037 kb on - strand, within IAI47_04745 at 1000.037 kb on - strand, within IAI47_04745 at 1000.037 kb on - strand, within IAI47_04745 at 1000.037 kb on - strand, within IAI47_04745 at 1000.037 kb on - strand, within IAI47_04745 at 1000.051 kb on - strand, within IAI47_04745 at 1000.051 kb on - strand, within IAI47_04745 at 1000.051 kb on - strand, within IAI47_04745 at 1000.088 kb on + strand at 1000.088 kb on + strand at 1000.088 kb on + strand at 1000.089 kb on - strand at 1000.089 kb on - strand at 1000.089 kb on - strand at 1000.091 kb on - strand at 1000.091 kb on - strand at 1000.243 kb on + strand, within IAI47_04750 at 1000.243 kb on + strand, within IAI47_04750 at 1000.244 kb on - strand, within IAI47_04750 at 1000.248 kb on + strand, within IAI47_04750 at 1000.274 kb on - strand, within IAI47_04750 at 1000.378 kb on + strand at 1000.378 kb on + strand at 1000.378 kb on + strand at 1000.379 kb on - strand at 1000.379 kb on - strand at 1000.379 kb on - strand at 1000.379 kb on - strand at 1000.379 kb on - strand at 1000.379 kb on - strand at 1000.379 kb on - strand at 1000.379 kb on - strand at 1000.379 kb on - strand at 1000.379 kb on - strand at 1000.389 kb on + strand at 1000.389 kb on + strand at 1000.389 kb on + strand at 1000.389 kb on + strand at 1000.504 kb on + strand, within IAI47_04755 at 1000.504 kb on + strand, within IAI47_04755 at 1000.504 kb on + strand, within IAI47_04755 at 1000.504 kb on + strand, within IAI47_04755 at 1000.505 kb on - strand, within IAI47_04755 at 1000.505 kb on - strand, within IAI47_04755 at 1000.511 kb on + strand, within IAI47_04755 at 1000.512 kb on - strand, within IAI47_04755 at 1000.563 kb on + strand, within IAI47_04755 at 1000.564 kb on - strand, within IAI47_04755 at 1000.564 kb on - strand, within IAI47_04755 at 1000.564 kb on - strand, within IAI47_04755 at 1000.564 kb on - strand, within IAI47_04755 at 1000.644 kb on + strand at 1000.644 kb on + strand at 1000.644 kb on + strand at 1000.645 kb on - strand at 1000.645 kb on - strand at 1000.652 kb on + strand at 1000.652 kb on + strand at 1000.652 kb on + strand at 1000.652 kb on + strand at 1000.653 kb on - strand at 1000.653 kb on - strand at 1000.653 kb on - strand at 1000.697 kb on - strand at 1000.697 kb on - strand at 1000.704 kb on + strand at 1000.713 kb on + strand at 1000.715 kb on + strand at 1000.715 kb on + strand at 1000.716 kb on - strand at 1000.716 kb on - strand at 1000.716 kb on - strand at 1000.716 kb on - strand at 1000.729 kb on + strand at 1000.729 kb on + strand at 1000.729 kb on + strand at 1000.729 kb on + strand at 1000.729 kb on + strand at 1000.729 kb on + strand at 1000.729 kb on + strand at 1000.730 kb on - strand at 1000.730 kb on - strand at 1000.730 kb on - strand at 1000.775 kb on + strand at 1000.775 kb on + strand at 1000.775 kb on + strand at 1000.775 kb on + strand at 1000.775 kb on + strand at 1000.776 kb on - strand at 1000.776 kb on - strand at 1000.823 kb on + strand at 1000.851 kb on + strand at 1000.852 kb on - strand at 1000.852 kb on - strand at 1000.899 kb on - strand at 1000.899 kb on - strand at 1000.926 kb on + strand at 1000.926 kb on + strand at 1000.927 kb on - strand at 1000.927 kb on - strand at 1000.942 kb on - strand at 1000.965 kb on - strand at 1000.974 kb on - strand at 1000.974 kb on - strand at 1000.977 kb on + strand at 1001.001 kb on + strand at 1001.015 kb on + strand at 1001.015 kb on + strand at 1001.015 kb on + strand at 1001.016 kb on - strand at 1001.016 kb on - strand at 1001.016 kb on - strand at 1001.016 kb on - strand at 1001.016 kb on - strand at 1001.016 kb on - strand at 1001.016 kb on - strand at 1001.017 kb on + strand at 1001.017 kb on + strand at 1001.017 kb on + strand at 1001.017 kb on + strand at 1001.018 kb on - strand at 1001.018 kb on - strand at 1001.018 kb on - strand at 1001.018 kb on - strand at 1001.026 kb on + strand at 1001.027 kb on - strand at 1001.115 kb on - strand at 1001.115 kb on - strand at 1001.139 kb on + strand at 1001.139 kb on + strand at 1001.140 kb on - strand at 1001.143 kb on + strand at 1001.223 kb on - strand at 1001.223 kb on - strand at 1001.241 kb on + strand, within IAI47_04760 at 1001.241 kb on + strand, within IAI47_04760 at 1001.241 kb on + strand, within IAI47_04760 at 1001.241 kb on + strand, within IAI47_04760 at 1001.242 kb on - strand, within IAI47_04760 at 1001.242 kb on - strand, within IAI47_04760 at 1001.242 kb on - strand, within IAI47_04760 at 1001.243 kb on + strand, within IAI47_04760 at 1001.261 kb on + strand, within IAI47_04760 at 1001.262 kb on - strand, within IAI47_04760 at 1001.263 kb on + strand, within IAI47_04760 at 1001.263 kb on + strand, within IAI47_04760 at 1001.263 kb on + strand, within IAI47_04760 at 1001.263 kb on + strand, within IAI47_04760 at 1001.263 kb on + strand, within IAI47_04760 at 1001.264 kb on - strand, within IAI47_04760 at 1001.264 kb on - strand, within IAI47_04760 at 1001.264 kb on - strand, within IAI47_04760 at 1001.264 kb on - strand, within IAI47_04760 at 1001.360 kb on + strand, within IAI47_04760 at 1001.360 kb on + strand, within IAI47_04760 at 1001.361 kb on - strand, within IAI47_04760 at 1001.361 kb on - strand, within IAI47_04760
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 999,185 + IAI47_04735 0.51 -0.2 999,185 + IAI47_04735 0.51 +0.5 999,185 + IAI47_04735 0.51 +0.8 999,185 + IAI47_04735 0.51 -0.0 999,186 - IAI47_04735 0.51 +2.0 999,186 - IAI47_04735 0.51 +2.4 999,186 - IAI47_04735 0.51 +0.6 999,186 - IAI47_04735 0.51 +1.2 999,186 - IAI47_04735 0.51 +1.0 999,186 - IAI47_04735 0.51 -1.7 999,186 - IAI47_04735 0.51 +1.6 999,186 - IAI47_04735 0.51 -0.7 999,275 + IAI47_04735 0.77 -1.8 999,300 - IAI47_04735 0.85 +0.2 999,313 + IAI47_04735 0.89 +0.8 999,313 + IAI47_04735 0.89 +0.5 999,313 + IAI47_04735 0.89 +1.0 999,314 - IAI47_04735 0.89 +0.9 999,369 + IAI47_04740 0.12 -0.5 999,369 - IAI47_04740 0.12 +0.6 999,543 + IAI47_04740 0.77 +0.5 999,543 + IAI47_04740 0.77 +1.4 999,544 - IAI47_04740 0.77 +0.4 999,544 - IAI47_04740 0.77 -0.2 999,564 + IAI47_04740 0.85 -0.5 999,564 + IAI47_04740 0.85 -1.0 999,564 + IAI47_04740 0.85 +0.4 999,564 + IAI47_04740 0.85 +0.4 999,598 + -1.8 999,598 + -0.0 999,598 + +0.5 999,598 + -0.4 999,598 + -0.1 999,599 - +1.0 999,601 + -0.3 999,601 + -0.5 999,601 + +1.3 999,602 - -0.5 999,602 - -0.4 999,602 - +0.5 999,602 - +0.2 999,602 - +1.0 999,650 + IAI47_04745 0.13 +0.3 999,650 + IAI47_04745 0.13 +0.7 999,651 - IAI47_04745 0.13 +0.8 999,651 - IAI47_04745 0.13 -0.3 999,651 - IAI47_04745 0.13 -0.3 999,699 + IAI47_04745 0.22 -0.8 999,700 - IAI47_04745 0.22 +1.4 999,708 + IAI47_04745 0.23 +3.7 999,708 + IAI47_04745 0.23 +0.3 999,708 + IAI47_04745 0.23 -1.4 999,708 + IAI47_04745 0.23 -1.9 999,709 - IAI47_04745 0.23 +0.4 999,709 - IAI47_04745 0.23 -0.2 999,709 - IAI47_04745 0.23 +1.3 999,709 - IAI47_04745 0.23 +0.8 999,753 + IAI47_04745 0.32 +0.4 999,753 + IAI47_04745 0.32 +0.2 999,754 - IAI47_04745 0.32 -0.2 999,754 - IAI47_04745 0.32 +1.2 999,780 + IAI47_04745 0.36 -0.0 999,780 + IAI47_04745 0.36 -2.8 999,795 + IAI47_04745 0.39 +0.4 999,795 + IAI47_04745 0.39 +0.2 999,795 + IAI47_04745 0.39 +2.2 999,795 + IAI47_04745 0.39 -1.4 999,795 + IAI47_04745 0.39 +0.1 999,795 + IAI47_04745 0.39 +0.4 999,796 - IAI47_04745 0.39 -0.8 999,814 + IAI47_04745 0.43 -0.0 999,814 + IAI47_04745 0.43 +0.3 999,815 - IAI47_04745 0.43 +1.4 999,820 + IAI47_04745 0.44 +0.4 999,820 + IAI47_04745 0.44 +0.2 999,820 + IAI47_04745 0.44 -0.5 999,820 + IAI47_04745 0.44 -1.4 999,820 + IAI47_04745 0.44 -0.0 999,820 + IAI47_04745 0.44 -1.5 999,820 + IAI47_04745 0.44 -1.0 999,821 - IAI47_04745 0.44 -1.7 999,822 + IAI47_04745 0.44 -0.2 999,823 - IAI47_04745 0.44 +0.0 999,823 - IAI47_04745 0.44 -1.5 999,829 - IAI47_04745 0.45 -0.4 999,829 - IAI47_04745 0.45 +1.3 999,829 - IAI47_04745 0.45 +0.1 999,843 + IAI47_04745 0.48 +1.0 999,844 - IAI47_04745 0.48 +1.7 999,844 - -0.8 999,844 - IAI47_04745 0.48 +1.1 999,874 - IAI47_04745 0.54 +1.8 999,935 - IAI47_04745 0.65 -0.3 999,935 - IAI47_04745 0.65 +1.4 1,000,036 + IAI47_04745 0.83 -1.6 1,000,037 - IAI47_04745 0.83 -0.5 1,000,037 - IAI47_04745 0.83 +1.7 1,000,037 - IAI47_04745 0.83 +0.7 1,000,037 - IAI47_04745 0.83 -0.0 1,000,037 - IAI47_04745 0.83 +0.8 1,000,051 - IAI47_04745 0.86 -1.1 1,000,051 - IAI47_04745 0.86 +0.4 1,000,051 - IAI47_04745 0.86 -1.6 1,000,088 + -1.2 1,000,088 + +1.1 1,000,088 + -1.1 1,000,089 - -0.0 1,000,089 - -0.3 1,000,089 - -0.0 1,000,091 - -0.9 1,000,091 - +0.5 1,000,243 + IAI47_04750 0.42 -1.4 1,000,243 + IAI47_04750 0.42 -0.5 1,000,244 - IAI47_04750 0.42 +0.4 1,000,248 + IAI47_04750 0.44 -0.0 1,000,274 - IAI47_04750 0.54 -1.1 1,000,378 + -0.3 1,000,378 + +0.7 1,000,378 + -1.0 1,000,379 - -0.3 1,000,379 - -0.2 1,000,379 - +0.1 1,000,379 - +3.0 1,000,379 - -1.2 1,000,379 - +0.6 1,000,379 - +0.5 1,000,379 - +0.4 1,000,379 - +0.5 1,000,379 - -0.4 1,000,389 + -0.4 1,000,389 + -0.4 1,000,389 + -0.7 1,000,389 + +1.3 1,000,504 + IAI47_04755 0.48 +0.2 1,000,504 + IAI47_04755 0.48 +0.1 1,000,504 + IAI47_04755 0.48 -0.4 1,000,504 + IAI47_04755 0.48 +0.3 1,000,505 - IAI47_04755 0.48 +0.4 1,000,505 - IAI47_04755 0.48 +0.6 1,000,511 + IAI47_04755 0.50 -0.2 1,000,512 - IAI47_04755 0.51 -0.5 1,000,563 + IAI47_04755 0.71 +0.2 1,000,564 - IAI47_04755 0.71 +0.6 1,000,564 - IAI47_04755 0.71 -1.9 1,000,564 - IAI47_04755 0.71 -1.0 1,000,564 - IAI47_04755 0.71 +1.1 1,000,644 + +0.8 1,000,644 + +0.8 1,000,644 + +0.5 1,000,645 - -0.5 1,000,645 - +0.4 1,000,652 + +1.2 1,000,652 + -0.0 1,000,652 + -0.8 1,000,652 + +2.5 1,000,653 - +0.5 1,000,653 - +0.2 1,000,653 - -0.4 1,000,697 - +1.0 1,000,697 - +3.0 1,000,704 + -0.0 1,000,713 + +1.0 1,000,715 + -0.2 1,000,715 + +0.3 1,000,716 - +0.2 1,000,716 - -0.4 1,000,716 - -0.1 1,000,716 - +0.0 1,000,729 + -0.6 1,000,729 + -0.8 1,000,729 + -0.5 1,000,729 + -0.1 1,000,729 + -0.9 1,000,729 + -0.9 1,000,729 + +3.1 1,000,730 - +1.6 1,000,730 - -0.0 1,000,730 - -0.4 1,000,775 + +1.8 1,000,775 + +0.4 1,000,775 + +0.0 1,000,775 + +0.1 1,000,775 + +0.8 1,000,776 - -0.5 1,000,776 - -0.8 1,000,823 + -0.9 1,000,851 + +1.1 1,000,852 - -0.2 1,000,852 - +0.9 1,000,899 - +2.5 1,000,899 - -0.9 1,000,926 + +1.6 1,000,926 + +1.7 1,000,927 - -0.6 1,000,927 - -0.6 1,000,942 - +0.1 1,000,965 - +0.1 1,000,974 - -0.8 1,000,974 - -0.1 1,000,977 + -1.0 1,001,001 + +0.5 1,001,015 + -0.7 1,001,015 + +0.4 1,001,015 + +1.2 1,001,016 - +0.6 1,001,016 - +0.8 1,001,016 - +0.2 1,001,016 - +0.5 1,001,016 - -0.2 1,001,016 - +2.4 1,001,016 - -0.0 1,001,017 + -2.1 1,001,017 + +0.0 1,001,017 + -0.4 1,001,017 + +1.2 1,001,018 - +0.1 1,001,018 - -0.6 1,001,018 - +1.0 1,001,018 - +0.1 1,001,026 + +0.4 1,001,027 - +0.5 1,001,115 - +0.5 1,001,115 - +2.5 1,001,139 + -0.2 1,001,139 + -1.8 1,001,140 - +0.1 1,001,143 + -1.2 1,001,223 - +0.5 1,001,223 - +0.2 1,001,241 + IAI47_04760 0.11 +0.4 1,001,241 + IAI47_04760 0.11 -0.8 1,001,241 + IAI47_04760 0.11 -0.9 1,001,241 + IAI47_04760 0.11 +0.8 1,001,242 - IAI47_04760 0.11 +1.0 1,001,242 - IAI47_04760 0.11 +0.5 1,001,242 - IAI47_04760 0.11 +0.7 1,001,243 + IAI47_04760 0.12 -0.4 1,001,261 + IAI47_04760 0.14 -1.0 1,001,262 - IAI47_04760 0.14 +0.6 1,001,263 + IAI47_04760 0.14 -0.8 1,001,263 + IAI47_04760 0.14 +0.3 1,001,263 + IAI47_04760 0.14 +1.4 1,001,263 + IAI47_04760 0.14 -0.8 1,001,263 + IAI47_04760 0.14 +0.1 1,001,264 - IAI47_04760 0.15 -0.2 1,001,264 - IAI47_04760 0.15 -0.2 1,001,264 - IAI47_04760 0.15 +2.3 1,001,264 - IAI47_04760 0.15 -0.8 1,001,360 + IAI47_04760 0.28 +5.8 1,001,360 + IAI47_04760 0.28 +1.2 1,001,361 - IAI47_04760 0.28 -1.3 1,001,361 - IAI47_04760 0.28 +0.6
Or see this region's nucleotide sequence