Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01720 and IAI47_01725 are separated by 135 nucleotides IAI47_01725 and IAI47_01730 overlap by 1 nucleotides IAI47_01730 and IAI47_01735 overlap by 8 nucleotides IAI47_01735 and IAI47_01740 are separated by 8 nucleotides
IAI47_01720: IAI47_01720 - AEC family transporter, at 376,435 to 377,394
_01720
IAI47_01725: IAI47_01725 - biotin-independent malonate decarboxylase subunit gamma, at 377,530 to 378,330
_01725
IAI47_01730: IAI47_01730 - biotin-independent malonate decarboxylase subunit beta, at 378,330 to 379,163
_01730
IAI47_01735: IAI47_01735 - malonate decarboxylase acyl carrier protein, at 379,156 to 379,455
_01735
IAI47_01740: IAI47_01740 - triphosphoribosyl-dephospho-CoA synthase, at 379,464 to 380,324
_01740
Position (kb) 378
379
380 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 377.341 kb on + strand at 377.341 kb on + strand at 377.342 kb on - strand at 377.342 kb on - strand at 377.349 kb on + strand at 377.349 kb on + strand at 377.349 kb on + strand at 377.349 kb on + strand at 377.349 kb on + strand at 377.349 kb on + strand at 377.349 kb on + strand at 377.350 kb on - strand at 377.350 kb on - strand at 377.350 kb on - strand at 377.350 kb on - strand at 377.350 kb on - strand at 377.351 kb on - strand at 377.355 kb on + strand at 377.356 kb on - strand at 377.383 kb on + strand at 377.383 kb on + strand at 377.383 kb on + strand at 377.383 kb on + strand at 377.383 kb on + strand at 377.384 kb on - strand at 377.384 kb on - strand at 377.384 kb on - strand at 377.384 kb on - strand at 377.384 kb on - strand at 377.387 kb on + strand at 377.387 kb on + strand at 377.388 kb on - strand at 377.388 kb on - strand at 377.388 kb on - strand at 377.394 kb on + strand at 377.395 kb on - strand at 377.395 kb on - strand at 377.405 kb on + strand at 377.405 kb on + strand at 377.405 kb on + strand at 377.410 kb on - strand at 377.441 kb on + strand at 377.442 kb on - strand at 377.442 kb on - strand at 377.444 kb on - strand at 377.482 kb on + strand at 377.483 kb on - strand at 377.483 kb on - strand at 377.523 kb on + strand at 377.523 kb on + strand at 377.523 kb on + strand at 377.531 kb on + strand at 377.532 kb on - strand at 377.532 kb on - strand at 377.532 kb on - strand at 377.547 kb on + strand at 377.547 kb on + strand at 377.547 kb on + strand at 377.547 kb on + strand at 377.547 kb on + strand at 377.547 kb on + strand at 377.547 kb on + strand at 377.548 kb on - strand at 377.548 kb on - strand at 377.548 kb on - strand at 377.548 kb on - strand at 377.548 kb on - strand at 377.548 kb on - strand at 377.580 kb on + strand at 377.580 kb on + strand at 377.580 kb on + strand at 377.580 kb on + strand at 377.581 kb on - strand at 377.607 kb on + strand at 377.607 kb on + strand at 377.641 kb on + strand, within IAI47_01725 at 377.641 kb on + strand, within IAI47_01725 at 377.641 kb on + strand, within IAI47_01725 at 377.642 kb on - strand, within IAI47_01725 at 377.642 kb on - strand, within IAI47_01725 at 377.642 kb on - strand, within IAI47_01725 at 377.642 kb on - strand, within IAI47_01725 at 377.642 kb on - strand, within IAI47_01725 at 377.642 kb on - strand, within IAI47_01725 at 377.642 kb on - strand, within IAI47_01725 at 377.736 kb on + strand, within IAI47_01725 at 377.736 kb on + strand, within IAI47_01725 at 377.737 kb on - strand, within IAI47_01725 at 377.737 kb on - strand, within IAI47_01725 at 377.737 kb on - strand, within IAI47_01725 at 377.737 kb on - strand, within IAI47_01725 at 377.747 kb on + strand, within IAI47_01725 at 377.747 kb on + strand, within IAI47_01725 at 377.747 kb on + strand, within IAI47_01725 at 377.747 kb on + strand, within IAI47_01725 at 377.747 kb on + strand, within IAI47_01725 at 377.748 kb on - strand, within IAI47_01725 at 377.748 kb on - strand, within IAI47_01725 at 377.748 kb on - strand, within IAI47_01725 at 377.748 kb on - strand, within IAI47_01725 at 377.748 kb on - strand, within IAI47_01725 at 377.755 kb on - strand, within IAI47_01725 at 377.919 kb on + strand, within IAI47_01725 at 377.919 kb on + strand, within IAI47_01725 at 377.920 kb on - strand, within IAI47_01725 at 377.920 kb on - strand, within IAI47_01725 at 378.029 kb on + strand, within IAI47_01725 at 378.029 kb on + strand, within IAI47_01725 at 378.030 kb on - strand, within IAI47_01725 at 378.030 kb on - strand, within IAI47_01725 at 378.030 kb on - strand, within IAI47_01725 at 378.030 kb on - strand, within IAI47_01725 at 378.170 kb on + strand, within IAI47_01725 at 378.202 kb on - strand, within IAI47_01725 at 378.222 kb on + strand, within IAI47_01725 at 378.222 kb on + strand, within IAI47_01725 at 378.222 kb on + strand, within IAI47_01725 at 378.223 kb on - strand, within IAI47_01725 at 378.331 kb on + strand at 378.331 kb on + strand at 378.331 kb on + strand at 378.331 kb on + strand at 378.332 kb on - strand at 378.332 kb on - strand at 378.332 kb on - strand at 378.360 kb on - strand at 378.360 kb on - strand at 378.424 kb on + strand, within IAI47_01730 at 378.424 kb on + strand, within IAI47_01730 at 378.424 kb on + strand, within IAI47_01730 at 378.424 kb on + strand, within IAI47_01730 at 378.425 kb on - strand, within IAI47_01730 at 378.425 kb on - strand, within IAI47_01730 at 378.425 kb on - strand, within IAI47_01730 at 378.425 kb on - strand, within IAI47_01730 at 378.428 kb on + strand, within IAI47_01730 at 378.428 kb on + strand, within IAI47_01730 at 378.428 kb on + strand, within IAI47_01730 at 378.428 kb on + strand, within IAI47_01730 at 378.428 kb on + strand, within IAI47_01730 at 378.429 kb on - strand, within IAI47_01730 at 378.429 kb on - strand, within IAI47_01730 at 378.429 kb on - strand, within IAI47_01730 at 378.429 kb on - strand, within IAI47_01730 at 378.429 kb on - strand, within IAI47_01730 at 378.429 kb on - strand, within IAI47_01730 at 378.429 kb on - strand, within IAI47_01730 at 378.465 kb on - strand, within IAI47_01730 at 378.465 kb on - strand, within IAI47_01730 at 378.498 kb on - strand, within IAI47_01730 at 378.498 kb on - strand, within IAI47_01730 at 378.498 kb on - strand, within IAI47_01730 at 378.566 kb on + strand, within IAI47_01730 at 378.566 kb on + strand, within IAI47_01730 at 378.589 kb on + strand, within IAI47_01730 at 378.589 kb on + strand, within IAI47_01730 at 378.589 kb on + strand, within IAI47_01730 at 378.589 kb on + strand, within IAI47_01730 at 378.589 kb on + strand, within IAI47_01730 at 378.590 kb on - strand, within IAI47_01730 at 378.590 kb on - strand, within IAI47_01730 at 378.590 kb on - strand, within IAI47_01730 at 378.590 kb on - strand, within IAI47_01730 at 378.667 kb on + strand, within IAI47_01730 at 378.667 kb on + strand, within IAI47_01730 at 378.668 kb on - strand, within IAI47_01730 at 378.668 kb on - strand, within IAI47_01730 at 378.668 kb on - strand, within IAI47_01730 at 378.668 kb on - strand, within IAI47_01730 at 378.689 kb on + strand, within IAI47_01730 at 378.689 kb on + strand, within IAI47_01730 at 378.690 kb on - strand, within IAI47_01730 at 378.745 kb on + strand, within IAI47_01730 at 378.745 kb on + strand, within IAI47_01730 at 378.746 kb on - strand, within IAI47_01730 at 378.777 kb on - strand, within IAI47_01730 at 379.090 kb on + strand at 379.134 kb on + strand at 379.135 kb on - strand at 379.143 kb on - strand at 379.157 kb on + strand at 379.157 kb on + strand at 379.157 kb on + strand at 379.158 kb on - strand at 379.191 kb on + strand, within IAI47_01735 at 379.192 kb on - strand, within IAI47_01735 at 379.192 kb on - strand, within IAI47_01735 at 379.192 kb on - strand, within IAI47_01735 at 379.198 kb on - strand, within IAI47_01735 at 379.233 kb on + strand, within IAI47_01735 at 379.234 kb on - strand, within IAI47_01735 at 379.234 kb on - strand, within IAI47_01735 at 379.264 kb on + strand, within IAI47_01735 at 379.264 kb on + strand, within IAI47_01735 at 379.265 kb on - strand, within IAI47_01735 at 379.265 kb on - strand, within IAI47_01735 at 379.265 kb on - strand, within IAI47_01735 at 379.265 kb on - strand, within IAI47_01735 at 379.326 kb on + strand, within IAI47_01735 at 379.327 kb on - strand, within IAI47_01735 at 379.361 kb on + strand, within IAI47_01735 at 379.361 kb on + strand, within IAI47_01735 at 379.362 kb on - strand, within IAI47_01735 at 379.362 kb on - strand, within IAI47_01735 at 379.374 kb on + strand, within IAI47_01735 at 379.374 kb on + strand, within IAI47_01735 at 379.375 kb on - strand, within IAI47_01735 at 379.375 kb on - strand, within IAI47_01735 at 379.425 kb on + strand, within IAI47_01735 at 379.426 kb on - strand at 379.432 kb on + strand at 379.433 kb on - strand at 379.438 kb on + strand at 379.438 kb on + strand at 379.438 kb on + strand at 379.438 kb on + strand at 379.438 kb on + strand at 379.438 kb on + strand at 379.439 kb on - strand at 379.439 kb on - strand at 379.439 kb on - strand at 379.439 kb on - strand at 379.439 kb on - strand at 379.439 kb on - strand at 379.455 kb on + strand at 379.456 kb on - strand at 379.465 kb on + strand at 379.471 kb on + strand at 379.471 kb on + strand at 379.472 kb on - strand at 379.472 kb on - strand at 379.691 kb on + strand, within IAI47_01740 at 379.691 kb on + strand, within IAI47_01740 at 379.846 kb on + strand, within IAI47_01740 at 379.847 kb on - strand, within IAI47_01740 at 379.863 kb on + strand, within IAI47_01740 at 379.863 kb on + strand, within IAI47_01740 at 379.864 kb on - strand, within IAI47_01740
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 377,341 + -1.5 377,341 + -0.7 377,342 - +0.2 377,342 - +0.9 377,349 + +3.4 377,349 + +0.1 377,349 + -0.1 377,349 + +0.3 377,349 + +0.8 377,349 + -1.3 377,349 + -2.0 377,350 - -0.4 377,350 - -0.9 377,350 - +0.3 377,350 - -1.7 377,350 - +1.2 377,351 - -0.0 377,355 + +0.3 377,356 - +0.8 377,383 + -0.9 377,383 + -0.4 377,383 + -1.2 377,383 + +0.5 377,383 + +0.7 377,384 - -0.3 377,384 - -1.3 377,384 - -0.0 377,384 - +0.3 377,384 - +0.7 377,387 + +0.9 377,387 + +0.4 377,388 - -1.0 377,388 - +1.1 377,388 - +0.2 377,394 + +0.6 377,395 - +2.2 377,395 - -0.9 377,405 + +0.7 377,405 + -0.9 377,405 + -0.3 377,410 - -0.1 377,441 + -0.5 377,442 - +1.3 377,442 - +0.4 377,444 - -0.4 377,482 + +0.1 377,483 - -0.6 377,483 - +0.3 377,523 + -0.3 377,523 + +0.5 377,523 + +0.7 377,531 + +0.7 377,532 - +0.3 377,532 - +0.5 377,532 - +1.1 377,547 + -1.1 377,547 + +0.1 377,547 + -2.0 377,547 + +0.8 377,547 + -0.4 377,547 + +2.8 377,547 + +0.6 377,548 - -1.4 377,548 - +0.3 377,548 - -0.3 377,548 - +1.0 377,548 - +1.1 377,548 - -0.4 377,580 + +0.4 377,580 + +1.1 377,580 + +1.3 377,580 + +0.0 377,581 - +1.1 377,607 + +0.8 377,607 + -0.0 377,641 + IAI47_01725 0.14 -0.6 377,641 + IAI47_01725 0.14 +0.8 377,641 + IAI47_01725 0.14 -1.2 377,642 - IAI47_01725 0.14 +0.6 377,642 - IAI47_01725 0.14 -2.5 377,642 - IAI47_01725 0.14 -1.9 377,642 - IAI47_01725 0.14 +0.4 377,642 - IAI47_01725 0.14 -1.4 377,642 - IAI47_01725 0.14 +2.2 377,642 - IAI47_01725 0.14 +0.7 377,736 + IAI47_01725 0.26 +2.8 377,736 + IAI47_01725 0.26 +0.2 377,737 - IAI47_01725 0.26 -1.1 377,737 - IAI47_01725 0.26 +0.7 377,737 - IAI47_01725 0.26 +0.0 377,737 - IAI47_01725 0.26 +2.1 377,747 + IAI47_01725 0.27 -0.5 377,747 + IAI47_01725 0.27 -2.4 377,747 + IAI47_01725 0.27 +0.3 377,747 + IAI47_01725 0.27 +0.9 377,747 + IAI47_01725 0.27 -0.2 377,748 - IAI47_01725 0.27 -0.2 377,748 - IAI47_01725 0.27 +1.9 377,748 - IAI47_01725 0.27 -1.1 377,748 - IAI47_01725 0.27 +0.3 377,748 - IAI47_01725 0.27 -0.5 377,755 - IAI47_01725 0.28 -0.9 377,919 + IAI47_01725 0.49 -1.4 377,919 + IAI47_01725 0.49 -0.7 377,920 - IAI47_01725 0.49 +1.6 377,920 - IAI47_01725 0.49 +0.7 378,029 + IAI47_01725 0.62 -0.0 378,029 + IAI47_01725 0.62 +3.9 378,030 - IAI47_01725 0.62 +1.5 378,030 - IAI47_01725 0.62 -0.8 378,030 - IAI47_01725 0.62 -0.9 378,030 - IAI47_01725 0.62 -0.1 378,170 + IAI47_01725 0.80 -0.3 378,202 - IAI47_01725 0.84 -0.3 378,222 + IAI47_01725 0.86 +0.5 378,222 + IAI47_01725 0.86 -0.3 378,222 + IAI47_01725 0.86 -2.3 378,223 - IAI47_01725 0.87 -0.6 378,331 + -1.7 378,331 + -1.0 378,331 + +0.7 378,331 + -2.2 378,332 - -0.2 378,332 - -0.8 378,332 - +0.2 378,360 - +0.7 378,360 - -1.1 378,424 + IAI47_01730 0.11 +0.5 378,424 + IAI47_01730 0.11 -0.3 378,424 + IAI47_01730 0.11 -0.6 378,424 + IAI47_01730 0.11 +0.4 378,425 - IAI47_01730 0.11 -0.0 378,425 - IAI47_01730 0.11 +0.9 378,425 - IAI47_01730 0.11 -0.4 378,425 - IAI47_01730 0.11 -0.2 378,428 + IAI47_01730 0.12 +0.1 378,428 + IAI47_01730 0.12 +0.2 378,428 + IAI47_01730 0.12 +0.4 378,428 + IAI47_01730 0.12 -0.0 378,428 + IAI47_01730 0.12 -1.6 378,429 - IAI47_01730 0.12 +0.2 378,429 - IAI47_01730 0.12 -1.2 378,429 - IAI47_01730 0.12 -1.6 378,429 - IAI47_01730 0.12 -0.0 378,429 - IAI47_01730 0.12 +0.5 378,429 - IAI47_01730 0.12 +0.8 378,429 - IAI47_01730 0.12 +0.6 378,465 - IAI47_01730 0.16 -0.5 378,465 - IAI47_01730 0.16 -1.1 378,498 - IAI47_01730 0.20 +2.7 378,498 - IAI47_01730 0.20 +1.0 378,498 - IAI47_01730 0.20 +1.2 378,566 + IAI47_01730 0.28 +0.4 378,566 + IAI47_01730 0.28 -0.8 378,589 + IAI47_01730 0.31 +0.5 378,589 + IAI47_01730 0.31 -1.7 378,589 + IAI47_01730 0.31 -1.9 378,589 + IAI47_01730 0.31 +1.3 378,589 + IAI47_01730 0.31 -0.5 378,590 - IAI47_01730 0.31 +0.1 378,590 - IAI47_01730 0.31 +0.5 378,590 - IAI47_01730 0.31 -0.0 378,590 - IAI47_01730 0.31 +1.3 378,667 + IAI47_01730 0.40 -0.9 378,667 + IAI47_01730 0.40 -2.2 378,668 - IAI47_01730 0.41 -0.9 378,668 - IAI47_01730 0.41 +0.7 378,668 - IAI47_01730 0.41 +1.5 378,668 - IAI47_01730 0.41 -2.0 378,689 + IAI47_01730 0.43 -1.5 378,689 + IAI47_01730 0.43 +0.3 378,690 - IAI47_01730 0.43 +1.0 378,745 + IAI47_01730 0.50 -1.2 378,745 + IAI47_01730 0.50 +4.8 378,746 - IAI47_01730 0.50 +0.5 378,777 - IAI47_01730 0.54 -0.1 379,090 + -1.0 379,134 + +0.9 379,135 - +0.1 379,143 - +1.3 379,157 + -0.2 379,157 + -1.6 379,157 + +0.3 379,158 - +0.2 379,191 + IAI47_01735 0.12 -0.7 379,192 - IAI47_01735 0.12 -0.7 379,192 - IAI47_01735 0.12 -0.3 379,192 - IAI47_01735 0.12 +0.3 379,198 - IAI47_01735 0.14 -0.4 379,233 + IAI47_01735 0.26 +0.0 379,234 - IAI47_01735 0.26 -0.9 379,234 - IAI47_01735 0.26 +1.7 379,264 + IAI47_01735 0.36 -1.2 379,264 + IAI47_01735 0.36 -1.0 379,265 - IAI47_01735 0.36 -0.3 379,265 - IAI47_01735 0.36 +0.3 379,265 - IAI47_01735 0.36 -0.8 379,265 - IAI47_01735 0.36 -0.2 379,326 + IAI47_01735 0.57 +0.1 379,327 - IAI47_01735 0.57 +0.2 379,361 + IAI47_01735 0.68 -0.2 379,361 + IAI47_01735 0.68 +1.8 379,362 - IAI47_01735 0.69 +0.3 379,362 - IAI47_01735 0.69 -0.0 379,374 + IAI47_01735 0.73 -0.7 379,374 + IAI47_01735 0.73 -0.4 379,375 - IAI47_01735 0.73 -1.7 379,375 - IAI47_01735 0.73 +0.1 379,425 + IAI47_01735 0.90 +0.7 379,426 - +0.7 379,432 + +0.2 379,433 - +1.4 379,438 + -0.4 379,438 + -0.5 379,438 + +0.4 379,438 + +0.5 379,438 + +1.7 379,438 + -0.1 379,439 - -1.5 379,439 - +0.1 379,439 - -0.2 379,439 - +0.7 379,439 - +0.5 379,439 - +2.2 379,455 + +0.2 379,456 - +1.1 379,465 + +1.1 379,471 + -0.4 379,471 + +0.5 379,472 - -0.3 379,472 - -2.2 379,691 + IAI47_01740 0.26 -2.3 379,691 + IAI47_01740 0.26 +1.0 379,846 + IAI47_01740 0.44 -2.2 379,847 - IAI47_01740 0.44 -0.7 379,863 + IAI47_01740 0.46 -0.8 379,863 + IAI47_01740 0.46 +0.6 379,864 - IAI47_01740 0.46 +0.3
Or see this region's nucleotide sequence