Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_21225 and IAI47_21230 are separated by 565 nucleotides IAI47_21230 and IAI47_21235 are separated by 112 nucleotides IAI47_21235 and IAI47_21240 are separated by 382 nucleotides
IAI47_21225: IAI47_21225 - phosphoethanolamine transferase EptA, at 502,006 to 503,655
_21225
IAI47_21230: IAI47_21230 - DUF4142 domain-containing protein, at 504,221 to 504,790
_21230
IAI47_21235: IAI47_21235 - hypothetical protein, at 504,903 to 505,061
_21235
IAI47_21240: IAI47_21240 - hypothetical protein, at 505,444 to 505,953
_21240
Position (kb) 504
505 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 503.223 kb on + strand, within IAI47_21225 at 503.224 kb on - strand, within IAI47_21225 at 503.224 kb on - strand, within IAI47_21225 at 503.259 kb on + strand, within IAI47_21225 at 503.262 kb on + strand, within IAI47_21225 at 503.262 kb on + strand, within IAI47_21225 at 503.262 kb on + strand, within IAI47_21225 at 503.262 kb on + strand, within IAI47_21225 at 503.262 kb on + strand, within IAI47_21225 at 503.262 kb on + strand, within IAI47_21225 at 503.263 kb on - strand, within IAI47_21225 at 503.263 kb on - strand, within IAI47_21225 at 503.263 kb on - strand, within IAI47_21225 at 503.263 kb on - strand, within IAI47_21225 at 503.263 kb on - strand, within IAI47_21225 at 503.263 kb on - strand, within IAI47_21225 at 503.263 kb on - strand, within IAI47_21225 at 503.263 kb on - strand, within IAI47_21225 at 503.263 kb on - strand, within IAI47_21225 at 503.266 kb on + strand, within IAI47_21225 at 503.266 kb on + strand, within IAI47_21225 at 503.266 kb on + strand, within IAI47_21225 at 503.267 kb on - strand, within IAI47_21225 at 503.267 kb on - strand, within IAI47_21225 at 503.267 kb on - strand, within IAI47_21225 at 503.284 kb on + strand, within IAI47_21225 at 503.285 kb on - strand, within IAI47_21225 at 503.293 kb on + strand, within IAI47_21225 at 503.293 kb on + strand, within IAI47_21225 at 503.293 kb on + strand, within IAI47_21225 at 503.294 kb on - strand, within IAI47_21225 at 503.294 kb on - strand, within IAI47_21225 at 503.294 kb on - strand, within IAI47_21225 at 503.294 kb on - strand, within IAI47_21225 at 503.295 kb on + strand, within IAI47_21225 at 503.296 kb on - strand, within IAI47_21225 at 503.296 kb on - strand, within IAI47_21225 at 503.304 kb on + strand, within IAI47_21225 at 503.359 kb on + strand, within IAI47_21225 at 503.359 kb on + strand, within IAI47_21225 at 503.359 kb on + strand, within IAI47_21225 at 503.360 kb on - strand, within IAI47_21225 at 503.360 kb on - strand, within IAI47_21225 at 503.401 kb on + strand, within IAI47_21225 at 503.401 kb on + strand, within IAI47_21225 at 503.401 kb on + strand, within IAI47_21225 at 503.401 kb on + strand, within IAI47_21225 at 503.401 kb on + strand, within IAI47_21225 at 503.401 kb on + strand, within IAI47_21225 at 503.401 kb on + strand, within IAI47_21225 at 503.402 kb on - strand, within IAI47_21225 at 503.402 kb on - strand, within IAI47_21225 at 503.419 kb on + strand, within IAI47_21225 at 503.420 kb on - strand, within IAI47_21225 at 503.424 kb on + strand, within IAI47_21225 at 503.460 kb on + strand, within IAI47_21225 at 503.460 kb on + strand, within IAI47_21225 at 503.460 kb on + strand, within IAI47_21225 at 503.460 kb on + strand, within IAI47_21225 at 503.461 kb on - strand, within IAI47_21225 at 503.461 kb on - strand, within IAI47_21225 at 503.461 kb on - strand, within IAI47_21225 at 503.492 kb on - strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.540 kb on - strand at 503.562 kb on + strand at 503.563 kb on - strand at 503.575 kb on - strand at 503.575 kb on - strand at 503.599 kb on + strand at 503.599 kb on + strand at 503.599 kb on + strand at 503.599 kb on + strand at 503.600 kb on - strand at 503.600 kb on - strand at 503.617 kb on - strand at 503.617 kb on - strand at 503.617 kb on - strand at 503.618 kb on + strand at 503.618 kb on + strand at 503.618 kb on + strand at 503.619 kb on - strand at 503.619 kb on - strand at 503.619 kb on - strand at 503.619 kb on - strand at 503.619 kb on - strand at 503.619 kb on - strand at 503.619 kb on - strand at 503.619 kb on - strand at 503.637 kb on + strand at 503.638 kb on - strand at 503.638 kb on - strand at 503.663 kb on + strand at 503.664 kb on - strand at 503.684 kb on - strand at 503.700 kb on - strand at 503.730 kb on - strand at 503.749 kb on + strand at 503.752 kb on + strand at 503.752 kb on + strand at 503.752 kb on + strand at 503.753 kb on - strand at 503.773 kb on + strand at 503.774 kb on - strand at 503.774 kb on - strand at 503.774 kb on - strand at 503.796 kb on + strand at 503.796 kb on + strand at 503.796 kb on + strand at 503.837 kb on + strand at 503.838 kb on - strand at 503.848 kb on + strand at 503.849 kb on - strand at 503.849 kb on - strand at 503.884 kb on + strand at 503.885 kb on - strand at 503.885 kb on - strand at 503.963 kb on + strand at 503.963 kb on + strand at 503.963 kb on + strand at 503.963 kb on + strand at 503.963 kb on + strand at 503.964 kb on - strand at 503.964 kb on - strand at 503.964 kb on - strand at 503.964 kb on - strand at 503.964 kb on - strand at 503.983 kb on + strand at 504.094 kb on + strand at 504.154 kb on - strand at 504.154 kb on - strand at 504.169 kb on + strand at 504.170 kb on - strand at 504.170 kb on - strand at 504.181 kb on + strand at 504.222 kb on + strand at 504.222 kb on + strand at 504.261 kb on - strand at 504.261 kb on - strand at 504.309 kb on - strand, within IAI47_21230 at 504.349 kb on + strand, within IAI47_21230 at 504.349 kb on + strand, within IAI47_21230 at 504.349 kb on + strand, within IAI47_21230 at 504.349 kb on + strand, within IAI47_21230 at 504.349 kb on + strand, within IAI47_21230 at 504.349 kb on + strand, within IAI47_21230 at 504.350 kb on - strand, within IAI47_21230 at 504.350 kb on - strand, within IAI47_21230 at 504.350 kb on - strand, within IAI47_21230 at 504.350 kb on - strand, within IAI47_21230 at 504.350 kb on - strand, within IAI47_21230 at 504.360 kb on - strand, within IAI47_21230 at 504.360 kb on - strand, within IAI47_21230 at 504.378 kb on + strand, within IAI47_21230 at 504.378 kb on + strand, within IAI47_21230 at 504.378 kb on + strand, within IAI47_21230 at 504.378 kb on + strand, within IAI47_21230 at 504.378 kb on + strand, within IAI47_21230 at 504.379 kb on - strand, within IAI47_21230 at 504.379 kb on - strand, within IAI47_21230 at 504.433 kb on + strand, within IAI47_21230 at 504.433 kb on + strand, within IAI47_21230 at 504.434 kb on - strand, within IAI47_21230 at 504.434 kb on - strand, within IAI47_21230 at 504.434 kb on - strand, within IAI47_21230 at 504.439 kb on + strand, within IAI47_21230 at 504.439 kb on + strand, within IAI47_21230 at 504.440 kb on - strand, within IAI47_21230 at 504.440 kb on - strand, within IAI47_21230 at 504.440 kb on - strand, within IAI47_21230 at 504.440 kb on - strand, within IAI47_21230 at 504.454 kb on + strand, within IAI47_21230 at 504.454 kb on + strand, within IAI47_21230 at 504.454 kb on + strand, within IAI47_21230 at 504.455 kb on - strand, within IAI47_21230 at 504.455 kb on - strand, within IAI47_21230 at 504.458 kb on + strand, within IAI47_21230 at 504.458 kb on + strand, within IAI47_21230 at 504.458 kb on + strand, within IAI47_21230 at 504.459 kb on - strand, within IAI47_21230 at 504.459 kb on - strand, within IAI47_21230 at 504.478 kb on + strand, within IAI47_21230 at 504.544 kb on + strand, within IAI47_21230 at 504.602 kb on - strand, within IAI47_21230 at 504.662 kb on - strand, within IAI47_21230 at 504.662 kb on - strand, within IAI47_21230 at 504.662 kb on - strand, within IAI47_21230 at 504.676 kb on + strand, within IAI47_21230 at 504.676 kb on + strand, within IAI47_21230 at 504.676 kb on + strand, within IAI47_21230 at 504.676 kb on + strand, within IAI47_21230 at 504.677 kb on - strand, within IAI47_21230 at 504.752 kb on + strand at 504.753 kb on - strand at 504.770 kb on - strand at 504.771 kb on + strand at 504.778 kb on + strand at 504.809 kb on - strand at 504.809 kb on - strand at 504.847 kb on + strand at 504.855 kb on + strand at 504.856 kb on - strand at 504.865 kb on - strand at 504.894 kb on + strand at 504.894 kb on + strand at 504.927 kb on + strand, within IAI47_21235 at 504.928 kb on - strand, within IAI47_21235 at 504.944 kb on - strand, within IAI47_21235 at 505.034 kb on + strand, within IAI47_21235 at 505.068 kb on + strand at 505.087 kb on + strand at 505.087 kb on + strand at 505.088 kb on - strand at 505.088 kb on - strand at 505.088 kb on - strand at 505.089 kb on + strand at 505.089 kb on + strand at 505.090 kb on - strand at 505.090 kb on - strand at 505.090 kb on - strand at 505.090 kb on - strand at 505.090 kb on - strand at 505.101 kb on - strand at 505.103 kb on + strand at 505.103 kb on + strand at 505.112 kb on + strand at 505.115 kb on + strand at 505.137 kb on + strand at 505.137 kb on + strand at 505.138 kb on - strand at 505.139 kb on + strand at 505.140 kb on - strand at 505.140 kb on - strand at 505.160 kb on - strand at 505.191 kb on - strand at 505.205 kb on - strand at 505.209 kb on - strand at 505.332 kb on + strand at 505.354 kb on + strand at 505.355 kb on - strand at 505.387 kb on + strand at 505.387 kb on + strand at 505.387 kb on + strand at 505.388 kb on - strand at 505.399 kb on + strand at 505.424 kb on + strand at 505.428 kb on - strand at 505.428 kb on - strand at 505.430 kb on + strand at 505.441 kb on - strand at 505.441 kb on - strand at 505.485 kb on + strand at 505.485 kb on + strand at 505.485 kb on + strand at 505.486 kb on - strand at 505.488 kb on - strand at 505.488 kb on - strand at 505.563 kb on + strand, within IAI47_21240 at 505.563 kb on + strand, within IAI47_21240 at 505.563 kb on + strand, within IAI47_21240 at 505.564 kb on - strand, within IAI47_21240 at 505.566 kb on + strand, within IAI47_21240 at 505.567 kb on - strand, within IAI47_21240 at 505.581 kb on + strand, within IAI47_21240 at 505.581 kb on + strand, within IAI47_21240 at 505.584 kb on + strand, within IAI47_21240 at 505.585 kb on - strand, within IAI47_21240 at 505.656 kb on + strand, within IAI47_21240 at 505.657 kb on - strand, within IAI47_21240 at 505.692 kb on + strand, within IAI47_21240 at 505.693 kb on - strand, within IAI47_21240 at 505.693 kb on - strand, within IAI47_21240 at 505.693 kb on - strand, within IAI47_21240 at 505.693 kb on - strand, within IAI47_21240 at 505.699 kb on + strand, within IAI47_21240 at 505.700 kb on - strand, within IAI47_21240 at 505.713 kb on + strand, within IAI47_21240 at 505.713 kb on + strand, within IAI47_21240 at 505.729 kb on + strand, within IAI47_21240 at 505.767 kb on - strand, within IAI47_21240
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 503,223 + IAI47_21225 0.74 -2.9 503,224 - IAI47_21225 0.74 -0.3 503,224 - IAI47_21225 0.74 +1.8 503,259 + IAI47_21225 0.76 -0.7 503,262 + IAI47_21225 0.76 -0.0 503,262 + IAI47_21225 0.76 +1.8 503,262 + IAI47_21225 0.76 +0.0 503,262 + IAI47_21225 0.76 -0.2 503,262 + IAI47_21225 0.76 +1.1 503,262 + IAI47_21225 0.76 -1.7 503,263 - IAI47_21225 0.76 +0.7 503,263 - IAI47_21225 0.76 -2.3 503,263 - IAI47_21225 0.76 -1.9 503,263 - IAI47_21225 0.76 -0.7 503,263 - IAI47_21225 0.76 +2.2 503,263 - IAI47_21225 0.76 +0.5 503,263 - IAI47_21225 0.76 -0.1 503,263 - IAI47_21225 0.76 +0.2 503,263 - IAI47_21225 0.76 -1.1 503,266 + IAI47_21225 0.76 -0.8 503,266 + IAI47_21225 0.76 -1.6 503,266 + IAI47_21225 0.76 -0.5 503,267 - IAI47_21225 0.76 +0.2 503,267 - IAI47_21225 0.76 -1.4 503,267 - IAI47_21225 0.76 +0.6 503,284 + IAI47_21225 0.77 -0.8 503,285 - IAI47_21225 0.78 +0.3 503,293 + IAI47_21225 0.78 +0.4 503,293 + IAI47_21225 0.78 -1.1 503,293 + IAI47_21225 0.78 -1.0 503,294 - IAI47_21225 0.78 -1.0 503,294 - IAI47_21225 0.78 +2.7 503,294 - IAI47_21225 0.78 -2.2 503,294 - IAI47_21225 0.78 +0.5 503,295 + IAI47_21225 0.78 -1.2 503,296 - IAI47_21225 0.78 -1.8 503,296 - IAI47_21225 0.78 +0.3 503,304 + IAI47_21225 0.79 +0.2 503,359 + IAI47_21225 0.82 +0.7 503,359 + IAI47_21225 0.82 +2.1 503,359 + IAI47_21225 0.82 +1.1 503,360 - IAI47_21225 0.82 -2.0 503,360 - IAI47_21225 0.82 +0.2 503,401 + IAI47_21225 0.85 -0.7 503,401 + IAI47_21225 0.85 -0.1 503,401 + IAI47_21225 0.85 -0.4 503,401 + IAI47_21225 0.85 -1.1 503,401 + IAI47_21225 0.85 +0.2 503,401 + IAI47_21225 0.85 +2.1 503,401 + IAI47_21225 0.85 +0.9 503,402 - IAI47_21225 0.85 +0.8 503,402 - IAI47_21225 0.85 +0.3 503,419 + IAI47_21225 0.86 +1.1 503,420 - IAI47_21225 0.86 +2.0 503,424 + IAI47_21225 0.86 +1.1 503,460 + IAI47_21225 0.88 -0.6 503,460 + IAI47_21225 0.88 -1.1 503,460 + IAI47_21225 0.88 -1.6 503,460 + IAI47_21225 0.88 -0.3 503,461 - IAI47_21225 0.88 +0.7 503,461 - IAI47_21225 0.88 +1.5 503,461 - IAI47_21225 0.88 -0.8 503,492 - +1.1 503,503 + +0.8 503,503 + -0.5 503,504 - +1.5 503,504 - +2.3 503,504 - -1.9 503,504 - +1.6 503,540 - -0.2 503,562 + +0.2 503,563 - +1.6 503,575 - -2.4 503,575 - -0.3 503,599 + +0.6 503,599 + -1.3 503,599 + -2.4 503,599 + -2.8 503,600 - +1.2 503,600 - -2.3 503,617 - +0.6 503,617 - -2.5 503,617 - -2.0 503,618 + -0.2 503,618 + +0.1 503,618 + +0.5 503,619 - +1.0 503,619 - -1.4 503,619 - +0.5 503,619 - +1.6 503,619 - +0.5 503,619 - +0.5 503,619 - +0.2 503,619 - +1.1 503,637 + -1.8 503,638 - +0.4 503,638 - -0.9 503,663 + +1.2 503,664 - +0.5 503,684 - -3.0 503,700 - +2.3 503,730 - +0.3 503,749 + +1.7 503,752 + +1.3 503,752 + -1.0 503,752 + +1.7 503,753 - +1.4 503,773 + -1.0 503,774 - +0.4 503,774 - +0.4 503,774 - +0.4 503,796 + +0.8 503,796 + +0.4 503,796 + -2.6 503,837 + +1.0 503,838 - +1.5 503,848 + -1.4 503,849 - -2.0 503,849 - +0.4 503,884 + -0.2 503,885 - -2.3 503,885 - -0.2 503,963 + +0.7 503,963 + -0.7 503,963 + -0.2 503,963 + -2.3 503,963 + +1.9 503,964 - +1.4 503,964 - -1.8 503,964 - -0.2 503,964 - -3.1 503,964 - -0.9 503,983 + -1.6 504,094 + +5.0 504,154 - -1.3 504,154 - +0.2 504,169 + -0.2 504,170 - -0.2 504,170 - +2.5 504,181 + -1.0 504,222 + +2.3 504,222 + -0.8 504,261 - -1.1 504,261 - +0.6 504,309 - IAI47_21230 0.15 -1.2 504,349 + IAI47_21230 0.22 -1.4 504,349 + IAI47_21230 0.22 -0.3 504,349 + IAI47_21230 0.22 -0.7 504,349 + IAI47_21230 0.22 +0.8 504,349 + IAI47_21230 0.22 -0.6 504,349 + IAI47_21230 0.22 +0.3 504,350 - IAI47_21230 0.23 -1.1 504,350 - IAI47_21230 0.23 +0.5 504,350 - IAI47_21230 0.23 -1.5 504,350 - IAI47_21230 0.23 +1.5 504,350 - IAI47_21230 0.23 -1.6 504,360 - IAI47_21230 0.24 -0.3 504,360 - IAI47_21230 0.24 -1.1 504,378 + IAI47_21230 0.28 -1.1 504,378 + IAI47_21230 0.28 -1.4 504,378 + IAI47_21230 0.28 -2.2 504,378 + IAI47_21230 0.28 +0.9 504,378 + IAI47_21230 0.28 -0.5 504,379 - IAI47_21230 0.28 +0.8 504,379 - IAI47_21230 0.28 -0.5 504,433 + IAI47_21230 0.37 -1.0 504,433 + IAI47_21230 0.37 +0.4 504,434 - IAI47_21230 0.37 -0.9 504,434 - IAI47_21230 0.37 +0.4 504,434 - IAI47_21230 0.37 -0.8 504,439 + IAI47_21230 0.38 +1.6 504,439 + IAI47_21230 0.38 -1.3 504,440 - IAI47_21230 0.38 -1.6 504,440 - IAI47_21230 0.38 -1.5 504,440 - IAI47_21230 0.38 +0.3 504,440 - IAI47_21230 0.38 -0.1 504,454 + IAI47_21230 0.41 +1.5 504,454 + IAI47_21230 0.41 -1.5 504,454 + IAI47_21230 0.41 +0.3 504,455 - IAI47_21230 0.41 -2.1 504,455 - IAI47_21230 0.41 +0.3 504,458 + IAI47_21230 0.42 -1.5 504,458 + IAI47_21230 0.42 -2.2 504,458 + IAI47_21230 0.42 +0.1 504,459 - IAI47_21230 0.42 +1.3 504,459 - IAI47_21230 0.42 -2.4 504,478 + IAI47_21230 0.45 -2.3 504,544 + IAI47_21230 0.57 -1.1 504,602 - IAI47_21230 0.67 -0.8 504,662 - IAI47_21230 0.77 +0.1 504,662 - IAI47_21230 0.77 +0.0 504,662 - IAI47_21230 0.77 -0.2 504,676 + IAI47_21230 0.80 +0.8 504,676 + IAI47_21230 0.80 +1.8 504,676 + IAI47_21230 0.80 -1.6 504,676 + IAI47_21230 0.80 -1.8 504,677 - IAI47_21230 0.80 +0.9 504,752 + +2.2 504,753 - -0.3 504,770 - -0.8 504,771 + -0.9 504,778 + +0.2 504,809 - +1.7 504,809 - +2.0 504,847 + +2.1 504,855 + +2.2 504,856 - -2.4 504,865 - +0.9 504,894 + -0.2 504,894 + +0.8 504,927 + IAI47_21235 0.15 -0.8 504,928 - IAI47_21235 0.16 -0.6 504,944 - IAI47_21235 0.26 +2.2 505,034 + IAI47_21235 0.82 -0.6 505,068 + +1.4 505,087 + +0.2 505,087 + +0.8 505,088 - -0.8 505,088 - -1.4 505,088 - -0.5 505,089 + +1.6 505,089 + -1.1 505,090 - -0.7 505,090 - -1.0 505,090 - -1.1 505,090 - -2.9 505,090 - -1.6 505,101 - +1.0 505,103 + -0.5 505,103 + -0.0 505,112 + -0.3 505,115 + +0.7 505,137 + -1.9 505,137 + -2.2 505,138 - +0.0 505,139 + -0.6 505,140 - +1.0 505,140 - +1.5 505,160 - -1.2 505,191 - -0.1 505,205 - +0.4 505,209 - +0.1 505,332 + -1.8 505,354 + -1.4 505,355 - +0.3 505,387 + +0.3 505,387 + -0.8 505,387 + +1.5 505,388 - +3.2 505,399 + +0.8 505,424 + -1.8 505,428 - +1.0 505,428 - -0.3 505,430 + -0.2 505,441 - -0.1 505,441 - -1.8 505,485 + +4.7 505,485 + +0.9 505,485 + -0.3 505,486 - -2.9 505,488 - +1.1 505,488 - -0.8 505,563 + IAI47_21240 0.23 -1.2 505,563 + IAI47_21240 0.23 -0.1 505,563 + IAI47_21240 0.23 -1.2 505,564 - IAI47_21240 0.24 -2.0 505,566 + IAI47_21240 0.24 +0.6 505,567 - IAI47_21240 0.24 +0.4 505,581 + IAI47_21240 0.27 +0.2 505,581 + IAI47_21240 0.27 +0.5 505,584 + IAI47_21240 0.27 -0.7 505,585 - IAI47_21240 0.28 +0.1 505,656 + IAI47_21240 0.42 +0.2 505,657 - IAI47_21240 0.42 -1.3 505,692 + IAI47_21240 0.49 +0.3 505,693 - IAI47_21240 0.49 +0.2 505,693 - IAI47_21240 0.49 -0.3 505,693 - IAI47_21240 0.49 -1.0 505,693 - IAI47_21240 0.49 +0.2 505,699 + IAI47_21240 0.50 +0.2 505,700 - IAI47_21240 0.50 -2.1 505,713 + IAI47_21240 0.53 +1.4 505,713 + IAI47_21240 0.53 -0.0 505,729 + IAI47_21240 0.56 -0.2 505,767 - IAI47_21240 0.63 -0.3
Or see this region's nucleotide sequence