Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19775 and IAI47_19780 are separated by 177 nucleotides IAI47_19780 and IAI47_19785 are separated by 114 nucleotides IAI47_19785 and IAI47_19790 overlap by 20 nucleotides
IAI47_19775: IAI47_19775 - MFS transporter, at 180,058 to 181,266
_19775
IAI47_19780: IAI47_19780 - TetR/AcrR family transcriptional regulator, at 181,444 to 182,061
_19780
IAI47_19785: IAI47_19785 - DUF1232 domain-containing protein, at 182,176 to 182,562
_19785
IAI47_19790: IAI47_19790 - sigma-54-dependent Fis family transcriptional regulator, at 182,543 to 184,504
_19790
Position (kb) 181
182
183 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 180.448 kb on + strand, within IAI47_19775 at 180.448 kb on + strand, within IAI47_19775 at 180.448 kb on + strand, within IAI47_19775 at 180.449 kb on - strand, within IAI47_19775 at 180.507 kb on + strand, within IAI47_19775 at 180.507 kb on + strand, within IAI47_19775 at 180.507 kb on + strand, within IAI47_19775 at 180.508 kb on - strand, within IAI47_19775 at 180.508 kb on - strand, within IAI47_19775 at 180.561 kb on + strand, within IAI47_19775 at 180.561 kb on + strand, within IAI47_19775 at 180.561 kb on + strand, within IAI47_19775 at 180.561 kb on + strand, within IAI47_19775 at 180.561 kb on + strand, within IAI47_19775 at 180.561 kb on + strand, within IAI47_19775 at 180.562 kb on - strand, within IAI47_19775 at 180.562 kb on - strand, within IAI47_19775 at 180.562 kb on - strand, within IAI47_19775 at 180.562 kb on - strand, within IAI47_19775 at 180.562 kb on - strand, within IAI47_19775 at 180.562 kb on - strand, within IAI47_19775 at 180.563 kb on + strand, within IAI47_19775 at 180.564 kb on - strand, within IAI47_19775 at 180.564 kb on - strand, within IAI47_19775 at 180.564 kb on - strand, within IAI47_19775 at 180.564 kb on - strand, within IAI47_19775 at 180.564 kb on - strand, within IAI47_19775 at 180.598 kb on - strand, within IAI47_19775 at 180.628 kb on - strand, within IAI47_19775 at 180.628 kb on - strand, within IAI47_19775 at 180.706 kb on - strand, within IAI47_19775 at 180.706 kb on - strand, within IAI47_19775 at 180.706 kb on - strand, within IAI47_19775 at 180.714 kb on + strand, within IAI47_19775 at 180.714 kb on + strand, within IAI47_19775 at 180.715 kb on - strand, within IAI47_19775 at 180.762 kb on + strand, within IAI47_19775 at 180.762 kb on + strand, within IAI47_19775 at 180.762 kb on + strand, within IAI47_19775 at 180.763 kb on - strand, within IAI47_19775 at 180.763 kb on - strand, within IAI47_19775 at 180.763 kb on - strand, within IAI47_19775 at 180.763 kb on - strand, within IAI47_19775 at 180.812 kb on - strand, within IAI47_19775 at 180.829 kb on - strand, within IAI47_19775 at 180.829 kb on - strand, within IAI47_19775 at 180.847 kb on + strand, within IAI47_19775 at 180.848 kb on - strand, within IAI47_19775 at 180.875 kb on + strand, within IAI47_19775 at 180.875 kb on + strand, within IAI47_19775 at 180.947 kb on + strand, within IAI47_19775 at 180.948 kb on - strand, within IAI47_19775 at 180.948 kb on - strand, within IAI47_19775 at 181.079 kb on + strand, within IAI47_19775 at 181.079 kb on + strand, within IAI47_19775 at 181.079 kb on + strand, within IAI47_19775 at 181.079 kb on + strand, within IAI47_19775 at 181.079 kb on + strand, within IAI47_19775 at 181.079 kb on + strand, within IAI47_19775 at 181.079 kb on + strand, within IAI47_19775 at 181.080 kb on - strand, within IAI47_19775 at 181.080 kb on - strand, within IAI47_19775 at 181.080 kb on - strand, within IAI47_19775 at 181.080 kb on - strand, within IAI47_19775 at 181.083 kb on + strand, within IAI47_19775 at 181.083 kb on + strand, within IAI47_19775 at 181.083 kb on + strand, within IAI47_19775 at 181.083 kb on + strand, within IAI47_19775 at 181.083 kb on + strand, within IAI47_19775 at 181.083 kb on + strand, within IAI47_19775 at 181.083 kb on + strand, within IAI47_19775 at 181.084 kb on - strand, within IAI47_19775 at 181.084 kb on - strand, within IAI47_19775 at 181.084 kb on - strand, within IAI47_19775 at 181.084 kb on - strand, within IAI47_19775 at 181.084 kb on - strand, within IAI47_19775 at 181.084 kb on - strand, within IAI47_19775 at 181.105 kb on + strand, within IAI47_19775 at 181.105 kb on + strand, within IAI47_19775 at 181.282 kb on - strand at 181.289 kb on + strand at 181.289 kb on + strand at 181.290 kb on - strand at 181.296 kb on + strand at 181.297 kb on - strand at 181.300 kb on + strand at 181.300 kb on + strand at 181.300 kb on + strand at 181.301 kb on - strand at 181.320 kb on + strand at 181.321 kb on - strand at 181.321 kb on - strand at 181.336 kb on - strand at 181.351 kb on + strand at 181.367 kb on + strand at 181.368 kb on - strand at 181.369 kb on + strand at 181.370 kb on - strand at 181.395 kb on + strand at 181.398 kb on - strand at 181.444 kb on - strand at 181.572 kb on + strand, within IAI47_19780 at 181.573 kb on - strand, within IAI47_19780 at 181.591 kb on + strand, within IAI47_19780 at 181.609 kb on - strand, within IAI47_19780 at 181.609 kb on - strand, within IAI47_19780 at 181.609 kb on - strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.644 kb on + strand, within IAI47_19780 at 181.644 kb on + strand, within IAI47_19780 at 181.644 kb on + strand, within IAI47_19780 at 181.644 kb on + strand, within IAI47_19780 at 181.645 kb on - strand, within IAI47_19780 at 181.645 kb on - strand, within IAI47_19780 at 181.654 kb on + strand, within IAI47_19780 at 181.654 kb on + strand, within IAI47_19780 at 181.654 kb on + strand, within IAI47_19780 at 181.788 kb on + strand, within IAI47_19780 at 181.791 kb on + strand, within IAI47_19780 at 181.791 kb on + strand, within IAI47_19780 at 181.791 kb on + strand, within IAI47_19780 at 181.845 kb on + strand, within IAI47_19780 at 181.845 kb on + strand, within IAI47_19780 at 181.846 kb on - strand, within IAI47_19780 at 181.992 kb on + strand, within IAI47_19780 at 182.041 kb on + strand at 182.041 kb on + strand at 182.041 kb on + strand at 182.041 kb on + strand at 182.042 kb on - strand at 182.049 kb on + strand at 182.049 kb on + strand at 182.050 kb on - strand at 182.050 kb on - strand at 182.115 kb on + strand at 182.115 kb on + strand at 182.115 kb on + strand at 182.116 kb on - strand at 182.116 kb on - strand at 182.128 kb on + strand at 182.128 kb on + strand at 182.144 kb on + strand at 182.144 kb on + strand at 182.175 kb on + strand at 182.175 kb on + strand at 182.175 kb on + strand at 182.175 kb on + strand at 182.175 kb on + strand at 182.176 kb on - strand at 182.176 kb on - strand at 182.229 kb on - strand, within IAI47_19785 at 182.253 kb on + strand, within IAI47_19785 at 182.253 kb on + strand, within IAI47_19785 at 182.254 kb on - strand, within IAI47_19785 at 182.297 kb on - strand, within IAI47_19785 at 182.347 kb on + strand, within IAI47_19785 at 182.369 kb on + strand, within IAI47_19785 at 182.370 kb on - strand, within IAI47_19785 at 182.400 kb on + strand, within IAI47_19785 at 182.400 kb on + strand, within IAI47_19785 at 182.401 kb on - strand, within IAI47_19785 at 182.401 kb on - strand, within IAI47_19785 at 182.401 kb on - strand, within IAI47_19785 at 182.401 kb on - strand, within IAI47_19785 at 182.451 kb on + strand, within IAI47_19785 at 182.452 kb on - strand, within IAI47_19785 at 182.452 kb on - strand, within IAI47_19785 at 182.571 kb on + strand at 182.571 kb on + strand at 182.599 kb on + strand at 182.599 kb on + strand at 182.599 kb on + strand at 182.599 kb on + strand at 182.599 kb on + strand at 182.600 kb on - strand at 182.600 kb on - strand at 182.600 kb on - strand at 182.600 kb on - strand at 182.600 kb on - strand at 182.600 kb on - strand at 182.600 kb on - strand at 182.601 kb on + strand at 182.601 kb on + strand at 182.601 kb on + strand at 182.601 kb on + strand at 182.601 kb on + strand at 182.601 kb on + strand at 182.601 kb on + strand at 182.601 kb on + strand at 182.602 kb on - strand at 182.602 kb on - strand at 182.602 kb on - strand at 182.602 kb on - strand at 182.602 kb on - strand at 182.603 kb on + strand at 182.603 kb on + strand at 182.603 kb on + strand at 182.603 kb on + strand at 182.603 kb on + strand at 182.603 kb on + strand at 182.604 kb on - strand at 182.604 kb on - strand at 182.604 kb on - strand at 182.676 kb on - strand at 182.676 kb on - strand at 182.680 kb on + strand at 182.680 kb on + strand at 182.681 kb on - strand at 182.681 kb on - strand at 182.834 kb on + strand, within IAI47_19790 at 182.899 kb on + strand, within IAI47_19790 at 182.900 kb on - strand, within IAI47_19790 at 182.900 kb on - strand, within IAI47_19790 at 182.900 kb on - strand, within IAI47_19790 at 182.936 kb on - strand, within IAI47_19790 at 182.952 kb on - strand, within IAI47_19790 at 182.997 kb on - strand, within IAI47_19790 at 183.009 kb on + strand, within IAI47_19790 at 183.012 kb on + strand, within IAI47_19790 at 183.012 kb on + strand, within IAI47_19790 at 183.012 kb on + strand, within IAI47_19790 at 183.012 kb on + strand, within IAI47_19790 at 183.013 kb on - strand, within IAI47_19790 at 183.013 kb on - strand, within IAI47_19790 at 183.013 kb on - strand, within IAI47_19790
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 180,448 + IAI47_19775 0.32 -0.5 180,448 + IAI47_19775 0.32 +3.1 180,448 + IAI47_19775 0.32 +0.1 180,449 - IAI47_19775 0.32 -0.7 180,507 + IAI47_19775 0.37 -1.5 180,507 + IAI47_19775 0.37 +1.0 180,507 + IAI47_19775 0.37 -1.5 180,508 - IAI47_19775 0.37 +1.9 180,508 - IAI47_19775 0.37 -1.0 180,561 + IAI47_19775 0.42 -0.0 180,561 + IAI47_19775 0.42 -1.2 180,561 + IAI47_19775 0.42 -2.1 180,561 + IAI47_19775 0.42 +2.7 180,561 + IAI47_19775 0.42 -1.1 180,561 + IAI47_19775 0.42 -0.7 180,562 - IAI47_19775 0.42 +0.5 180,562 - IAI47_19775 0.42 +0.1 180,562 - IAI47_19775 0.42 -0.9 180,562 - IAI47_19775 0.42 -0.1 180,562 - IAI47_19775 0.42 -0.4 180,562 - IAI47_19775 0.42 -2.9 180,563 + IAI47_19775 0.42 -1.5 180,564 - IAI47_19775 0.42 +0.7 180,564 - IAI47_19775 0.42 -1.9 180,564 - IAI47_19775 0.42 +2.5 180,564 - IAI47_19775 0.42 +2.2 180,564 - IAI47_19775 0.42 +0.3 180,598 - IAI47_19775 0.45 -1.5 180,628 - IAI47_19775 0.47 -0.5 180,628 - IAI47_19775 0.47 +0.6 180,706 - IAI47_19775 0.54 +1.1 180,706 - IAI47_19775 0.54 -1.6 180,706 - IAI47_19775 0.54 +0.5 180,714 + IAI47_19775 0.54 -0.1 180,714 + IAI47_19775 0.54 -0.1 180,715 - IAI47_19775 0.54 +0.3 180,762 + IAI47_19775 0.58 -1.5 180,762 + IAI47_19775 0.58 -0.3 180,762 + IAI47_19775 0.58 +0.7 180,763 - IAI47_19775 0.58 -1.5 180,763 - IAI47_19775 0.58 -1.3 180,763 - IAI47_19775 0.58 -0.9 180,763 - IAI47_19775 0.58 -1.8 180,812 - IAI47_19775 0.62 -0.1 180,829 - IAI47_19775 0.64 +1.1 180,829 - IAI47_19775 0.64 +0.6 180,847 + IAI47_19775 0.65 -2.5 180,848 - IAI47_19775 0.65 +0.6 180,875 + IAI47_19775 0.68 +2.9 180,875 + IAI47_19775 0.68 +0.2 180,947 + IAI47_19775 0.74 +1.1 180,948 - IAI47_19775 0.74 +0.5 180,948 - IAI47_19775 0.74 -1.3 181,079 + IAI47_19775 0.84 +0.7 181,079 + IAI47_19775 0.84 -1.8 181,079 + IAI47_19775 0.84 -1.8 181,079 + IAI47_19775 0.84 +1.1 181,079 + IAI47_19775 0.84 +0.5 181,079 + IAI47_19775 0.84 +1.9 181,079 + IAI47_19775 0.84 -1.5 181,080 - IAI47_19775 0.85 -0.1 181,080 - IAI47_19775 0.85 +2.3 181,080 - IAI47_19775 0.85 -0.4 181,080 - IAI47_19775 0.85 +0.5 181,083 + IAI47_19775 0.85 +0.9 181,083 + IAI47_19775 0.85 -0.4 181,083 + IAI47_19775 0.85 +0.5 181,083 + IAI47_19775 0.85 +1.0 181,083 + IAI47_19775 0.85 +1.2 181,083 + IAI47_19775 0.85 +0.4 181,083 + IAI47_19775 0.85 -0.9 181,084 - IAI47_19775 0.85 -0.2 181,084 - IAI47_19775 0.85 +0.1 181,084 - IAI47_19775 0.85 +0.5 181,084 - IAI47_19775 0.85 -0.2 181,084 - IAI47_19775 0.85 +0.1 181,084 - IAI47_19775 0.85 -2.4 181,105 + IAI47_19775 0.87 -0.4 181,105 + IAI47_19775 0.87 -1.6 181,282 - +1.3 181,289 + +1.0 181,289 + +0.8 181,290 - -2.1 181,296 + +0.6 181,297 - -0.4 181,300 + -1.5 181,300 + +1.1 181,300 + -0.0 181,301 - -0.4 181,320 + +1.1 181,321 - +1.0 181,321 - +0.4 181,336 - -1.7 181,351 + -0.0 181,367 + +1.0 181,368 - -0.4 181,369 + +2.7 181,370 - +0.5 181,395 + +1.9 181,398 - +0.9 181,444 - -0.5 181,572 + IAI47_19780 0.21 -0.9 181,573 - IAI47_19780 0.21 -0.8 181,591 + IAI47_19780 0.24 -2.4 181,609 - IAI47_19780 0.27 -2.0 181,609 - IAI47_19780 0.27 +1.1 181,609 - IAI47_19780 0.27 -1.2 181,642 + IAI47_19780 0.32 -1.0 181,642 + IAI47_19780 0.32 -1.4 181,642 + IAI47_19780 0.32 -2.6 181,642 + IAI47_19780 0.32 -1.9 181,642 + IAI47_19780 0.32 -1.7 181,642 + IAI47_19780 0.32 +1.5 181,642 + IAI47_19780 0.32 +1.4 181,642 + IAI47_19780 0.32 -1.0 181,644 + IAI47_19780 0.32 -1.6 181,644 + IAI47_19780 0.32 +0.9 181,644 + IAI47_19780 0.32 -0.3 181,644 + IAI47_19780 0.32 -0.2 181,645 - IAI47_19780 0.33 +1.3 181,645 - IAI47_19780 0.33 +0.8 181,654 + IAI47_19780 0.34 -1.8 181,654 + IAI47_19780 0.34 -0.5 181,654 + IAI47_19780 0.34 +0.5 181,788 + IAI47_19780 0.56 -1.2 181,791 + IAI47_19780 0.56 -0.9 181,791 + IAI47_19780 0.56 -1.9 181,791 + IAI47_19780 0.56 -1.5 181,845 + IAI47_19780 0.65 -0.9 181,845 + IAI47_19780 0.65 +1.0 181,846 - IAI47_19780 0.65 -0.1 181,992 + IAI47_19780 0.89 +2.5 182,041 + +0.1 182,041 + +1.7 182,041 + -1.0 182,041 + -0.5 182,042 - -0.1 182,049 + +2.3 182,049 + -2.5 182,050 - +5.7 182,050 - +0.3 182,115 + +0.2 182,115 + -1.2 182,115 + +0.5 182,116 - +0.5 182,116 - -0.6 182,128 + -0.3 182,128 + +0.7 182,144 + -2.2 182,144 + +2.4 182,175 + +0.0 182,175 + -0.4 182,175 + -0.1 182,175 + +0.2 182,175 + +0.5 182,176 - -1.5 182,176 - +0.5 182,229 - IAI47_19785 0.14 +0.6 182,253 + IAI47_19785 0.20 -1.9 182,253 + IAI47_19785 0.20 -0.1 182,254 - IAI47_19785 0.20 -0.7 182,297 - IAI47_19785 0.31 -1.1 182,347 + IAI47_19785 0.44 -0.4 182,369 + IAI47_19785 0.50 -0.7 182,370 - IAI47_19785 0.50 -0.4 182,400 + IAI47_19785 0.58 +0.5 182,400 + IAI47_19785 0.58 -0.1 182,401 - IAI47_19785 0.58 -0.5 182,401 - IAI47_19785 0.58 -0.1 182,401 - IAI47_19785 0.58 -2.4 182,401 - IAI47_19785 0.58 +1.2 182,451 + IAI47_19785 0.71 -1.0 182,452 - IAI47_19785 0.71 +1.9 182,452 - IAI47_19785 0.71 -0.9 182,571 + -1.4 182,571 + -0.5 182,599 + -1.4 182,599 + -0.4 182,599 + +0.3 182,599 + +1.3 182,599 + +0.9 182,600 - -2.5 182,600 - -1.1 182,600 - +1.3 182,600 - +0.5 182,600 - -1.2 182,600 - +0.7 182,600 - -1.0 182,601 + -0.5 182,601 + +0.5 182,601 + +0.1 182,601 + +0.5 182,601 + -0.4 182,601 + -1.5 182,601 + +1.9 182,601 + -1.9 182,602 - +0.7 182,602 - -0.5 182,602 - +1.5 182,602 - +0.6 182,602 - +1.2 182,603 + -0.2 182,603 + +0.7 182,603 + -1.2 182,603 + -1.2 182,603 + +2.1 182,603 + +1.6 182,604 - +2.2 182,604 - +1.0 182,604 - +1.0 182,676 - -0.5 182,676 - -0.2 182,680 + -1.2 182,680 + -0.7 182,681 - +1.9 182,681 - +0.0 182,834 + IAI47_19790 0.15 +3.1 182,899 + IAI47_19790 0.18 -0.3 182,900 - IAI47_19790 0.18 +0.9 182,900 - IAI47_19790 0.18 -0.4 182,900 - IAI47_19790 0.18 -0.1 182,936 - IAI47_19790 0.20 -0.4 182,952 - IAI47_19790 0.21 -0.9 182,997 - IAI47_19790 0.23 +0.0 183,009 + IAI47_19790 0.24 -0.5 183,012 + IAI47_19790 0.24 -0.5 183,012 + IAI47_19790 0.24 -1.5 183,012 + IAI47_19790 0.24 +0.9 183,012 + IAI47_19790 0.24 -0.3 183,013 - IAI47_19790 0.24 -2.0 183,013 - IAI47_19790 0.24 -0.7 183,013 - IAI47_19790 0.24 +0.4
Or see this region's nucleotide sequence