Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_14195 and IAI47_14200 are separated by 225 nucleotides IAI47_14200 and IAI47_14205 are separated by 182 nucleotides IAI47_14205 and IAI47_14210 are separated by 94 nucleotides
IAI47_14195: IAI47_14195 - D-alanyl-D-alanine dipeptidase, at 2,998,653 to 2,999,234
_14195
IAI47_14200: IAI47_14200 - hypothetical protein, at 2,999,460 to 2,999,951
_14200
IAI47_14205: IAI47_14205 - hypothetical protein, at 3,000,134 to 3,001,099
_14205
IAI47_14210: IAI47_14210 - hypothetical protein, at 3,001,194 to 3,002,099
_14210
Position (kb)
3000
3001
3002 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2999.134 kb on + strand, within IAI47_14195 at 2999.145 kb on - strand, within IAI47_14195 at 2999.170 kb on + strand, within IAI47_14195 at 2999.170 kb on + strand, within IAI47_14195 at 2999.210 kb on + strand at 2999.211 kb on - strand at 2999.211 kb on - strand at 2999.213 kb on + strand at 2999.263 kb on + strand at 2999.275 kb on - strand at 2999.304 kb on - strand at 2999.315 kb on + strand at 2999.338 kb on + strand at 2999.390 kb on + strand at 2999.523 kb on - strand, within IAI47_14200 at 2999.634 kb on + strand, within IAI47_14200 at 2999.664 kb on - strand, within IAI47_14200 at 2999.666 kb on + strand, within IAI47_14200 at 2999.829 kb on + strand, within IAI47_14200 at 2999.829 kb on + strand, within IAI47_14200 at 2999.830 kb on - strand, within IAI47_14200 at 2999.909 kb on + strand at 3000.030 kb on - strand at 3000.212 kb on - strand at 3000.214 kb on - strand at 3000.228 kb on + strand at 3000.232 kb on + strand, within IAI47_14205 at 3000.232 kb on + strand, within IAI47_14205 at 3000.302 kb on + strand, within IAI47_14205 at 3000.361 kb on + strand, within IAI47_14205 at 3000.441 kb on + strand, within IAI47_14205 at 3000.441 kb on + strand, within IAI47_14205 at 3000.442 kb on - strand, within IAI47_14205 at 3000.448 kb on + strand, within IAI47_14205 at 3000.474 kb on - strand, within IAI47_14205 at 3000.583 kb on + strand, within IAI47_14205 at 3000.623 kb on + strand, within IAI47_14205 at 3000.719 kb on - strand, within IAI47_14205 at 3000.727 kb on + strand, within IAI47_14205 at 3000.727 kb on + strand, within IAI47_14205 at 3000.784 kb on + strand, within IAI47_14205 at 3000.789 kb on + strand, within IAI47_14205 at 3000.790 kb on - strand, within IAI47_14205 at 3000.793 kb on + strand, within IAI47_14205 at 3000.802 kb on + strand, within IAI47_14205 at 3000.803 kb on - strand, within IAI47_14205 at 3000.808 kb on - strand, within IAI47_14205 at 3000.808 kb on - strand, within IAI47_14205 at 3000.815 kb on + strand, within IAI47_14205 at 3000.815 kb on + strand, within IAI47_14205 at 3000.815 kb on + strand, within IAI47_14205 at 3000.816 kb on - strand, within IAI47_14205 at 3001.009 kb on - strand at 3001.028 kb on - strand at 3001.041 kb on - strand at 3001.059 kb on - strand at 3001.115 kb on - strand at 3001.119 kb on - strand at 3001.159 kb on + strand at 3001.160 kb on - strand at 3001.160 kb on - strand at 3001.160 kb on - strand at 3001.166 kb on - strand at 3001.166 kb on - strand at 3001.166 kb on - strand at 3001.199 kb on + strand at 3001.199 kb on + strand at 3001.200 kb on - strand at 3001.207 kb on + strand at 3001.207 kb on + strand at 3001.207 kb on + strand at 3001.207 kb on + strand at 3001.208 kb on - strand at 3001.208 kb on - strand at 3001.208 kb on - strand at 3001.272 kb on + strand at 3001.311 kb on - strand, within IAI47_14210 at 3001.313 kb on + strand, within IAI47_14210 at 3001.319 kb on + strand, within IAI47_14210 at 3001.320 kb on - strand, within IAI47_14210 at 3001.384 kb on + strand, within IAI47_14210 at 3001.385 kb on - strand, within IAI47_14210 at 3001.408 kb on - strand, within IAI47_14210 at 3001.474 kb on + strand, within IAI47_14210 at 3001.474 kb on + strand, within IAI47_14210 at 3001.474 kb on + strand, within IAI47_14210 at 3001.679 kb on + strand, within IAI47_14210 at 3001.794 kb on - strand, within IAI47_14210 at 3001.839 kb on - strand, within IAI47_14210 at 3001.839 kb on - strand, within IAI47_14210 at 3001.867 kb on - strand, within IAI47_14210 at 3001.998 kb on + strand, within IAI47_14210
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,999,134 + IAI47_14195 0.83 +0.2 2,999,145 - IAI47_14195 0.85 +3.6 2,999,170 + IAI47_14195 0.89 -0.5 2,999,170 + IAI47_14195 0.89 +1.2 2,999,210 + +2.7 2,999,211 - -0.7 2,999,211 - +1.5 2,999,213 + -2.3 2,999,263 + -1.5 2,999,275 - -0.2 2,999,304 - -0.3 2,999,315 + -0.8 2,999,338 + -0.1 2,999,390 + +1.4 2,999,523 - IAI47_14200 0.13 +2.3 2,999,634 + IAI47_14200 0.35 -2.2 2,999,664 - IAI47_14200 0.41 +3.5 2,999,666 + IAI47_14200 0.42 -1.7 2,999,829 + IAI47_14200 0.75 +0.1 2,999,829 + IAI47_14200 0.75 +0.2 2,999,830 - IAI47_14200 0.75 -2.4 2,999,909 + -2.3 3,000,030 - -0.2 3,000,212 - +2.1 3,000,214 - +0.0 3,000,228 + +3.5 3,000,232 + IAI47_14205 0.10 -0.3 3,000,232 + IAI47_14205 0.10 -2.4 3,000,302 + IAI47_14205 0.17 +2.3 3,000,361 + IAI47_14205 0.23 -2.6 3,000,441 + IAI47_14205 0.32 -1.4 3,000,441 + IAI47_14205 0.32 +0.3 3,000,442 - IAI47_14205 0.32 -2.9 3,000,448 + IAI47_14205 0.33 +0.5 3,000,474 - IAI47_14205 0.35 +0.2 3,000,583 + IAI47_14205 0.46 +2.1 3,000,623 + IAI47_14205 0.51 -1.2 3,000,719 - IAI47_14205 0.61 -2.8 3,000,727 + IAI47_14205 0.61 -2.2 3,000,727 + IAI47_14205 0.61 -0.7 3,000,784 + IAI47_14205 0.67 -1.7 3,000,789 + IAI47_14205 0.68 +0.9 3,000,790 - IAI47_14205 0.68 +0.6 3,000,793 + IAI47_14205 0.68 +1.4 3,000,802 + IAI47_14205 0.69 +0.9 3,000,803 - IAI47_14205 0.69 -2.5 3,000,808 - IAI47_14205 0.70 -0.8 3,000,808 - IAI47_14205 0.70 +2.1 3,000,815 + IAI47_14205 0.70 +1.7 3,000,815 + IAI47_14205 0.70 +0.9 3,000,815 + IAI47_14205 0.70 +0.1 3,000,816 - IAI47_14205 0.71 -0.1 3,001,009 - -2.1 3,001,028 - -2.2 3,001,041 - +2.2 3,001,059 - +0.7 3,001,115 - +1.5 3,001,119 - -0.3 3,001,159 + -0.3 3,001,160 - -0.9 3,001,160 - -1.0 3,001,160 - -0.5 3,001,166 - +0.5 3,001,166 - +0.5 3,001,166 - +0.0 3,001,199 + +1.2 3,001,199 + +0.2 3,001,200 - -1.2 3,001,207 + -1.3 3,001,207 + +0.9 3,001,207 + +0.5 3,001,207 + -1.3 3,001,208 - +2.3 3,001,208 - +0.7 3,001,208 - -0.1 3,001,272 + -2.6 3,001,311 - IAI47_14210 0.13 +2.2 3,001,313 + IAI47_14210 0.13 +0.9 3,001,319 + IAI47_14210 0.14 +1.4 3,001,320 - IAI47_14210 0.14 +3.4 3,001,384 + IAI47_14210 0.21 -1.7 3,001,385 - IAI47_14210 0.21 -0.7 3,001,408 - IAI47_14210 0.24 -0.4 3,001,474 + IAI47_14210 0.31 -2.0 3,001,474 + IAI47_14210 0.31 -0.4 3,001,474 + IAI47_14210 0.31 +0.7 3,001,679 + IAI47_14210 0.54 +1.1 3,001,794 - IAI47_14210 0.66 -0.5 3,001,839 - IAI47_14210 0.71 -1.5 3,001,839 - IAI47_14210 0.71 +0.9 3,001,867 - IAI47_14210 0.74 -1.2 3,001,998 + IAI47_14210 0.89 -0.8
Or see this region's nucleotide sequence