Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_14190 and IAI47_14195 are separated by 167 nucleotides IAI47_14195 and IAI47_14200 are separated by 225 nucleotides IAI47_14200 and IAI47_14205 are separated by 182 nucleotides
IAI47_14190: IAI47_14190 - DUF2171 domain-containing protein, at 2,998,249 to 2,998,485
_14190
IAI47_14195: IAI47_14195 - D-alanyl-D-alanine dipeptidase, at 2,998,653 to 2,999,234
_14195
IAI47_14200: IAI47_14200 - hypothetical protein, at 2,999,460 to 2,999,951
_14200
IAI47_14205: IAI47_14205 - hypothetical protein, at 3,000,134 to 3,001,099
_14205
Position (kb)
2999
3000 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2998.479 kb on + strand at 2998.483 kb on + strand at 2998.515 kb on - strand at 2998.542 kb on - strand at 2998.593 kb on + strand at 2998.593 kb on + strand at 2998.629 kb on + strand at 2998.629 kb on + strand at 2998.630 kb on - strand at 2998.631 kb on + strand at 2998.632 kb on - strand at 2998.632 kb on - strand at 2998.644 kb on + strand at 2998.645 kb on - strand at 2998.646 kb on + strand at 2998.687 kb on + strand at 2998.688 kb on - strand at 2998.688 kb on - strand at 2998.688 kb on - strand at 2998.688 kb on - strand at 2998.717 kb on + strand, within IAI47_14195 at 2998.718 kb on - strand, within IAI47_14195 at 2998.718 kb on - strand, within IAI47_14195 at 2998.718 kb on - strand, within IAI47_14195 at 2998.718 kb on - strand, within IAI47_14195 at 2998.764 kb on - strand, within IAI47_14195 at 2998.796 kb on + strand, within IAI47_14195 at 2998.796 kb on + strand, within IAI47_14195 at 2998.796 kb on + strand, within IAI47_14195 at 2998.796 kb on + strand, within IAI47_14195 at 2998.797 kb on - strand, within IAI47_14195 at 2998.797 kb on - strand, within IAI47_14195 at 2998.797 kb on - strand, within IAI47_14195 at 2998.843 kb on + strand, within IAI47_14195 at 2998.843 kb on + strand, within IAI47_14195 at 2998.843 kb on + strand, within IAI47_14195 at 2998.843 kb on + strand, within IAI47_14195 at 2998.844 kb on - strand, within IAI47_14195 at 2998.844 kb on - strand, within IAI47_14195 at 2998.844 kb on - strand, within IAI47_14195 at 2998.855 kb on + strand, within IAI47_14195 at 2998.855 kb on + strand, within IAI47_14195 at 2998.855 kb on + strand, within IAI47_14195 at 2998.856 kb on - strand, within IAI47_14195 at 2998.856 kb on - strand, within IAI47_14195 at 2998.856 kb on - strand, within IAI47_14195 at 2998.887 kb on - strand, within IAI47_14195 at 2998.926 kb on + strand, within IAI47_14195 at 2998.926 kb on + strand, within IAI47_14195 at 2998.975 kb on + strand, within IAI47_14195 at 2998.975 kb on + strand, within IAI47_14195 at 2998.975 kb on + strand, within IAI47_14195 at 2998.975 kb on + strand, within IAI47_14195 at 2998.976 kb on - strand, within IAI47_14195 at 2998.976 kb on - strand, within IAI47_14195 at 2998.976 kb on - strand, within IAI47_14195 at 2998.976 kb on - strand, within IAI47_14195 at 2998.976 kb on - strand, within IAI47_14195 at 2998.976 kb on - strand, within IAI47_14195 at 2998.976 kb on - strand, within IAI47_14195 at 2998.976 kb on - strand, within IAI47_14195 at 2999.051 kb on + strand, within IAI47_14195 at 2999.051 kb on + strand, within IAI47_14195 at 2999.051 kb on + strand, within IAI47_14195 at 2999.051 kb on + strand, within IAI47_14195 at 2999.051 kb on + strand, within IAI47_14195 at 2999.051 kb on + strand, within IAI47_14195 at 2999.051 kb on + strand, within IAI47_14195 at 2999.052 kb on - strand, within IAI47_14195 at 2999.052 kb on - strand, within IAI47_14195 at 2999.052 kb on - strand, within IAI47_14195 at 2999.052 kb on - strand, within IAI47_14195 at 2999.053 kb on + strand, within IAI47_14195 at 2999.072 kb on + strand, within IAI47_14195 at 2999.078 kb on + strand, within IAI47_14195 at 2999.078 kb on + strand, within IAI47_14195 at 2999.079 kb on - strand, within IAI47_14195 at 2999.079 kb on - strand, within IAI47_14195 at 2999.134 kb on + strand, within IAI47_14195 at 2999.145 kb on - strand, within IAI47_14195 at 2999.170 kb on + strand, within IAI47_14195 at 2999.170 kb on + strand, within IAI47_14195 at 2999.210 kb on + strand at 2999.211 kb on - strand at 2999.211 kb on - strand at 2999.213 kb on + strand at 2999.263 kb on + strand at 2999.275 kb on - strand at 2999.304 kb on - strand at 2999.315 kb on + strand at 2999.338 kb on + strand at 2999.390 kb on + strand at 2999.523 kb on - strand, within IAI47_14200 at 2999.634 kb on + strand, within IAI47_14200 at 2999.664 kb on - strand, within IAI47_14200 at 2999.666 kb on + strand, within IAI47_14200 at 2999.829 kb on + strand, within IAI47_14200 at 2999.829 kb on + strand, within IAI47_14200 at 2999.830 kb on - strand, within IAI47_14200 at 2999.909 kb on + strand at 3000.030 kb on - strand at 3000.212 kb on - strand at 3000.214 kb on - strand at 3000.228 kb on + strand at 3000.232 kb on + strand, within IAI47_14205 at 3000.232 kb on + strand, within IAI47_14205 at 3000.302 kb on + strand, within IAI47_14205 at 3000.361 kb on + strand, within IAI47_14205 at 3000.441 kb on + strand, within IAI47_14205 at 3000.441 kb on + strand, within IAI47_14205 at 3000.442 kb on - strand, within IAI47_14205 at 3000.448 kb on + strand, within IAI47_14205 at 3000.474 kb on - strand, within IAI47_14205 at 3000.583 kb on + strand, within IAI47_14205 at 3000.623 kb on + strand, within IAI47_14205 at 3000.719 kb on - strand, within IAI47_14205 at 3000.727 kb on + strand, within IAI47_14205 at 3000.727 kb on + strand, within IAI47_14205 at 3000.784 kb on + strand, within IAI47_14205 at 3000.789 kb on + strand, within IAI47_14205 at 3000.790 kb on - strand, within IAI47_14205 at 3000.793 kb on + strand, within IAI47_14205 at 3000.802 kb on + strand, within IAI47_14205 at 3000.803 kb on - strand, within IAI47_14205 at 3000.808 kb on - strand, within IAI47_14205 at 3000.808 kb on - strand, within IAI47_14205 at 3000.815 kb on + strand, within IAI47_14205 at 3000.815 kb on + strand, within IAI47_14205 at 3000.815 kb on + strand, within IAI47_14205 at 3000.816 kb on - strand, within IAI47_14205
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,998,479 + -0.4 2,998,483 + -1.0 2,998,515 - -0.1 2,998,542 - -1.2 2,998,593 + -0.7 2,998,593 + -0.0 2,998,629 + -1.0 2,998,629 + +1.0 2,998,630 - +0.1 2,998,631 + +0.7 2,998,632 - +0.4 2,998,632 - +2.1 2,998,644 + +2.9 2,998,645 - -1.3 2,998,646 + +0.3 2,998,687 + -0.4 2,998,688 - +2.1 2,998,688 - +0.5 2,998,688 - +3.0 2,998,688 - -1.3 2,998,717 + IAI47_14195 0.11 -2.8 2,998,718 - IAI47_14195 0.11 -0.7 2,998,718 - IAI47_14195 0.11 -0.8 2,998,718 - IAI47_14195 0.11 -0.1 2,998,718 - IAI47_14195 0.11 +0.3 2,998,764 - IAI47_14195 0.19 +0.2 2,998,796 + IAI47_14195 0.25 -1.0 2,998,796 + IAI47_14195 0.25 +1.3 2,998,796 + IAI47_14195 0.25 -2.6 2,998,796 + IAI47_14195 0.25 -0.7 2,998,797 - IAI47_14195 0.25 +1.7 2,998,797 - IAI47_14195 0.25 -0.3 2,998,797 - IAI47_14195 0.25 +1.4 2,998,843 + IAI47_14195 0.33 +0.9 2,998,843 + IAI47_14195 0.33 -0.6 2,998,843 + IAI47_14195 0.33 -0.3 2,998,843 + IAI47_14195 0.33 +0.6 2,998,844 - IAI47_14195 0.33 -0.9 2,998,844 - IAI47_14195 0.33 -1.5 2,998,844 - IAI47_14195 0.33 -1.4 2,998,855 + IAI47_14195 0.35 -1.8 2,998,855 + IAI47_14195 0.35 +2.0 2,998,855 + IAI47_14195 0.35 -0.2 2,998,856 - IAI47_14195 0.35 +1.5 2,998,856 - IAI47_14195 0.35 +0.4 2,998,856 - IAI47_14195 0.35 +0.4 2,998,887 - IAI47_14195 0.40 -0.7 2,998,926 + IAI47_14195 0.47 +0.3 2,998,926 + IAI47_14195 0.47 +2.9 2,998,975 + IAI47_14195 0.55 +2.0 2,998,975 + IAI47_14195 0.55 -3.4 2,998,975 + IAI47_14195 0.55 +0.9 2,998,975 + IAI47_14195 0.55 +1.0 2,998,976 - IAI47_14195 0.55 +1.8 2,998,976 - IAI47_14195 0.55 -2.3 2,998,976 - IAI47_14195 0.55 -1.9 2,998,976 - IAI47_14195 0.55 +0.4 2,998,976 - IAI47_14195 0.55 -1.7 2,998,976 - IAI47_14195 0.55 +0.8 2,998,976 - IAI47_14195 0.55 -0.4 2,998,976 - IAI47_14195 0.55 -2.0 2,999,051 + IAI47_14195 0.68 +1.3 2,999,051 + IAI47_14195 0.68 +1.0 2,999,051 + IAI47_14195 0.68 -0.7 2,999,051 + IAI47_14195 0.68 +0.8 2,999,051 + IAI47_14195 0.68 +0.4 2,999,051 + IAI47_14195 0.68 -1.1 2,999,051 + IAI47_14195 0.68 -0.8 2,999,052 - IAI47_14195 0.69 -0.5 2,999,052 - IAI47_14195 0.69 +0.2 2,999,052 - IAI47_14195 0.69 +0.4 2,999,052 - IAI47_14195 0.69 +0.9 2,999,053 + IAI47_14195 0.69 -0.4 2,999,072 + IAI47_14195 0.72 +0.2 2,999,078 + IAI47_14195 0.73 -0.0 2,999,078 + IAI47_14195 0.73 -1.5 2,999,079 - IAI47_14195 0.73 -2.2 2,999,079 - IAI47_14195 0.73 +1.2 2,999,134 + IAI47_14195 0.83 +0.2 2,999,145 - IAI47_14195 0.85 +3.6 2,999,170 + IAI47_14195 0.89 -0.5 2,999,170 + IAI47_14195 0.89 +1.2 2,999,210 + +2.7 2,999,211 - -0.7 2,999,211 - +1.5 2,999,213 + -2.3 2,999,263 + -1.5 2,999,275 - -0.2 2,999,304 - -0.3 2,999,315 + -0.8 2,999,338 + -0.1 2,999,390 + +1.4 2,999,523 - IAI47_14200 0.13 +2.3 2,999,634 + IAI47_14200 0.35 -2.2 2,999,664 - IAI47_14200 0.41 +3.5 2,999,666 + IAI47_14200 0.42 -1.7 2,999,829 + IAI47_14200 0.75 +0.1 2,999,829 + IAI47_14200 0.75 +0.2 2,999,830 - IAI47_14200 0.75 -2.4 2,999,909 + -2.3 3,000,030 - -0.2 3,000,212 - +2.1 3,000,214 - +0.0 3,000,228 + +3.5 3,000,232 + IAI47_14205 0.10 -0.3 3,000,232 + IAI47_14205 0.10 -2.4 3,000,302 + IAI47_14205 0.17 +2.3 3,000,361 + IAI47_14205 0.23 -2.6 3,000,441 + IAI47_14205 0.32 -1.4 3,000,441 + IAI47_14205 0.32 +0.3 3,000,442 - IAI47_14205 0.32 -2.9 3,000,448 + IAI47_14205 0.33 +0.5 3,000,474 - IAI47_14205 0.35 +0.2 3,000,583 + IAI47_14205 0.46 +2.1 3,000,623 + IAI47_14205 0.51 -1.2 3,000,719 - IAI47_14205 0.61 -2.8 3,000,727 + IAI47_14205 0.61 -2.2 3,000,727 + IAI47_14205 0.61 -0.7 3,000,784 + IAI47_14205 0.67 -1.7 3,000,789 + IAI47_14205 0.68 +0.9 3,000,790 - IAI47_14205 0.68 +0.6 3,000,793 + IAI47_14205 0.68 +1.4 3,000,802 + IAI47_14205 0.69 +0.9 3,000,803 - IAI47_14205 0.69 -2.5 3,000,808 - IAI47_14205 0.70 -0.8 3,000,808 - IAI47_14205 0.70 +2.1 3,000,815 + IAI47_14205 0.70 +1.7 3,000,815 + IAI47_14205 0.70 +0.9 3,000,815 + IAI47_14205 0.70 +0.1 3,000,816 - IAI47_14205 0.71 -0.1
Or see this region's nucleotide sequence