Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_13690 and IAI47_13695 are separated by 27 nucleotides IAI47_13695 and IAI47_13700 are separated by 13 nucleotides IAI47_13700 and IAI47_13705 overlap by 4 nucleotides IAI47_13705 and IAI47_13710 are separated by 178 nucleotides IAI47_13710 and IAI47_13715 overlap by 1 nucleotides IAI47_13715 and IAI47_13720 are separated by 15 nucleotides
IAI47_13690: IAI47_13690 - cell envelope integrity protein TolA, at 2,899,658 to 2,900,992
_13690
IAI47_13695: IAI47_13695 - colicin uptake protein TolR, at 2,901,020 to 2,901,448
_13695
IAI47_13700: IAI47_13700 - Tol-Pal system protein TolQ, at 2,901,462 to 2,902,148
_13700
IAI47_13705: IAI47_13705 - tol-pal system-associated acyl-CoA thioesterase, at 2,902,145 to 2,902,549
_13705
IAI47_13710: IAI47_13710 - cyd operon protein YbgE, at 2,902,728 to 2,903,021
_13710
IAI47_13715: IAI47_13715 - cytochrome bd-I oxidase subunit CydX, at 2,903,021 to 2,903,125
_13715
IAI47_13720: IAI47_13720 - cytochrome d ubiquinol oxidase subunit II, at 2,903,141 to 2,904,280
_13720
Position (kb)
2901
2902
2903 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2901.022 kb on - strand at 2901.022 kb on - strand at 2901.386 kb on + strand, within IAI47_13695 at 2901.460 kb on - strand at 2901.460 kb on - strand at 2901.460 kb on - strand at 2901.464 kb on - strand at 2901.464 kb on - strand at 2901.464 kb on - strand at 2901.468 kb on - strand at 2901.468 kb on - strand at 2901.468 kb on - strand at 2901.474 kb on - strand at 2901.574 kb on + strand, within IAI47_13700 at 2901.575 kb on - strand, within IAI47_13700 at 2901.575 kb on - strand, within IAI47_13700 at 2901.690 kb on - strand, within IAI47_13700 at 2901.734 kb on - strand, within IAI47_13700 at 2901.734 kb on - strand, within IAI47_13700 at 2901.837 kb on - strand, within IAI47_13700 at 2901.887 kb on + strand, within IAI47_13700 at 2901.953 kb on - strand, within IAI47_13700 at 2901.953 kb on - strand, within IAI47_13700 at 2902.029 kb on - strand, within IAI47_13700 at 2902.047 kb on - strand, within IAI47_13700 at 2902.047 kb on - strand, within IAI47_13700 at 2902.092 kb on - strand at 2902.092 kb on - strand at 2902.092 kb on - strand at 2902.092 kb on - strand at 2902.168 kb on + strand at 2902.169 kb on - strand at 2902.169 kb on - strand at 2902.169 kb on - strand at 2902.169 kb on - strand at 2902.196 kb on + strand, within IAI47_13705 at 2902.197 kb on - strand, within IAI47_13705 at 2902.197 kb on - strand, within IAI47_13705 at 2902.197 kb on - strand, within IAI47_13705 at 2902.197 kb on - strand, within IAI47_13705 at 2902.208 kb on - strand, within IAI47_13705 at 2902.208 kb on - strand, within IAI47_13705 at 2902.208 kb on - strand, within IAI47_13705 at 2902.208 kb on - strand, within IAI47_13705 at 2902.256 kb on - strand, within IAI47_13705 at 2902.354 kb on - strand, within IAI47_13705 at 2902.354 kb on - strand, within IAI47_13705 at 2902.354 kb on - strand, within IAI47_13705 at 2902.354 kb on - strand, within IAI47_13705 at 2902.397 kb on + strand, within IAI47_13705 at 2902.398 kb on - strand, within IAI47_13705 at 2902.442 kb on - strand, within IAI47_13705 at 2902.467 kb on + strand, within IAI47_13705 at 2902.468 kb on - strand, within IAI47_13705 at 2902.468 kb on - strand, within IAI47_13705 at 2902.468 kb on - strand, within IAI47_13705 at 2902.468 kb on - strand, within IAI47_13705 at 2902.468 kb on - strand, within IAI47_13705 at 2902.501 kb on + strand, within IAI47_13705 at 2902.502 kb on - strand, within IAI47_13705 at 2902.502 kb on - strand, within IAI47_13705 at 2902.502 kb on - strand, within IAI47_13705 at 2902.509 kb on + strand at 2902.510 kb on - strand at 2902.544 kb on - strand at 2902.544 kb on - strand at 2902.552 kb on + strand at 2902.553 kb on - strand at 2902.553 kb on - strand at 2902.553 kb on - strand at 2902.553 kb on - strand at 2902.553 kb on - strand at 2902.571 kb on - strand at 2902.580 kb on + strand at 2902.581 kb on - strand at 2902.581 kb on - strand at 2902.581 kb on - strand at 2902.581 kb on - strand at 2902.581 kb on - strand at 2902.583 kb on + strand at 2902.583 kb on + strand at 2902.584 kb on - strand at 2902.584 kb on - strand at 2902.584 kb on - strand at 2902.584 kb on - strand at 2902.584 kb on - strand at 2902.585 kb on + strand at 2902.585 kb on + strand at 2902.585 kb on + strand at 2902.586 kb on - strand at 2902.586 kb on - strand at 2902.634 kb on + strand at 2902.635 kb on - strand at 2902.635 kb on - strand at 2902.635 kb on - strand at 2902.675 kb on + strand at 2902.676 kb on - strand at 2902.697 kb on + strand at 2902.697 kb on + strand at 2902.697 kb on + strand at 2902.698 kb on - strand at 2902.698 kb on - strand at 2902.698 kb on - strand at 2902.729 kb on + strand at 2902.729 kb on + strand at 2902.729 kb on + strand at 2902.729 kb on + strand at 2902.729 kb on + strand at 2902.729 kb on + strand at 2902.730 kb on - strand at 2902.730 kb on - strand at 2902.730 kb on - strand at 2902.730 kb on - strand at 2902.730 kb on - strand at 2902.743 kb on + strand at 2902.755 kb on - strand at 2902.758 kb on + strand, within IAI47_13710 at 2902.759 kb on - strand, within IAI47_13710 at 2902.759 kb on - strand, within IAI47_13710 at 2902.759 kb on - strand, within IAI47_13710 at 2902.759 kb on - strand, within IAI47_13710 at 2902.759 kb on - strand, within IAI47_13710 at 2902.784 kb on + strand, within IAI47_13710 at 2902.826 kb on + strand, within IAI47_13710 at 2902.827 kb on - strand, within IAI47_13710 at 2902.972 kb on - strand, within IAI47_13710 at 2902.996 kb on + strand at 2902.996 kb on + strand at 2902.996 kb on + strand at 2902.996 kb on + strand at 2902.996 kb on + strand at 2902.996 kb on + strand at 2902.997 kb on - strand at 2902.997 kb on - strand at 2902.997 kb on - strand at 2903.015 kb on + strand at 2903.015 kb on + strand at 2903.016 kb on - strand at 2903.016 kb on - strand at 2903.016 kb on - strand at 2903.074 kb on + strand, within IAI47_13715 at 2903.139 kb on - strand at 2903.139 kb on - strand at 2903.143 kb on - strand at 2903.143 kb on - strand at 2903.143 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,901,022 - -1.2 2,901,022 - +0.2 2,901,386 + IAI47_13695 0.85 -2.2 2,901,460 - +0.1 2,901,460 - +1.5 2,901,460 - +0.8 2,901,464 - -0.9 2,901,464 - +0.8 2,901,464 - -1.6 2,901,468 - +1.1 2,901,468 - -1.2 2,901,468 - -0.1 2,901,474 - +1.8 2,901,574 + IAI47_13700 0.16 -0.2 2,901,575 - IAI47_13700 0.16 -1.2 2,901,575 - IAI47_13700 0.16 +0.1 2,901,690 - IAI47_13700 0.33 -1.0 2,901,734 - IAI47_13700 0.40 -2.3 2,901,734 - IAI47_13700 0.40 -0.2 2,901,837 - IAI47_13700 0.55 -1.5 2,901,887 + IAI47_13700 0.62 -3.2 2,901,953 - IAI47_13700 0.71 -3.2 2,901,953 - IAI47_13700 0.71 -2.2 2,902,029 - IAI47_13700 0.83 -1.0 2,902,047 - IAI47_13700 0.85 -2.0 2,902,047 - IAI47_13700 0.85 -1.7 2,902,092 - -2.2 2,902,092 - +1.1 2,902,092 - -0.9 2,902,092 - -2.1 2,902,168 + -1.9 2,902,169 - +0.3 2,902,169 - +0.3 2,902,169 - +0.1 2,902,169 - -1.5 2,902,196 + IAI47_13705 0.13 -2.7 2,902,197 - IAI47_13705 0.13 -1.7 2,902,197 - IAI47_13705 0.13 +0.3 2,902,197 - IAI47_13705 0.13 -2.1 2,902,197 - IAI47_13705 0.13 -0.4 2,902,208 - IAI47_13705 0.16 -0.9 2,902,208 - IAI47_13705 0.16 -1.7 2,902,208 - IAI47_13705 0.16 -0.1 2,902,208 - IAI47_13705 0.16 -0.3 2,902,256 - IAI47_13705 0.27 +0.7 2,902,354 - IAI47_13705 0.52 +0.5 2,902,354 - IAI47_13705 0.52 -1.7 2,902,354 - IAI47_13705 0.52 -2.1 2,902,354 - IAI47_13705 0.52 -1.9 2,902,397 + IAI47_13705 0.62 -3.1 2,902,398 - IAI47_13705 0.62 +0.8 2,902,442 - IAI47_13705 0.73 +0.1 2,902,467 + IAI47_13705 0.80 -1.5 2,902,468 - IAI47_13705 0.80 -0.1 2,902,468 - IAI47_13705 0.80 +1.3 2,902,468 - IAI47_13705 0.80 +0.9 2,902,468 - IAI47_13705 0.80 +0.6 2,902,468 - IAI47_13705 0.80 -0.6 2,902,501 + IAI47_13705 0.88 -2.9 2,902,502 - IAI47_13705 0.88 -0.9 2,902,502 - IAI47_13705 0.88 +1.2 2,902,502 - IAI47_13705 0.88 +0.5 2,902,509 + -0.8 2,902,510 - +0.8 2,902,544 - +1.5 2,902,544 - +0.0 2,902,552 + -2.3 2,902,553 - +0.3 2,902,553 - -1.8 2,902,553 - +0.1 2,902,553 - +2.2 2,902,553 - -0.3 2,902,571 - -0.0 2,902,580 + -1.1 2,902,581 - +1.9 2,902,581 - -0.9 2,902,581 - +0.3 2,902,581 - -0.5 2,902,581 - +0.1 2,902,583 + -2.5 2,902,583 + -2.2 2,902,584 - -1.0 2,902,584 - -0.5 2,902,584 - +1.2 2,902,584 - -1.1 2,902,584 - +0.3 2,902,585 + -1.5 2,902,585 + -2.2 2,902,585 + -1.0 2,902,586 - -0.5 2,902,586 - +0.2 2,902,634 + +1.0 2,902,635 - -0.7 2,902,635 - +0.2 2,902,635 - -1.4 2,902,675 + -1.8 2,902,676 - -0.3 2,902,697 + -0.2 2,902,697 + +1.1 2,902,697 + +1.0 2,902,698 - +0.7 2,902,698 - -0.5 2,902,698 - -1.0 2,902,729 + +0.1 2,902,729 + +1.7 2,902,729 + +0.1 2,902,729 + +1.4 2,902,729 + +1.3 2,902,729 + -1.9 2,902,730 - -1.7 2,902,730 - -0.5 2,902,730 - +0.6 2,902,730 - +0.8 2,902,730 - +1.4 2,902,743 + -2.4 2,902,755 - +2.4 2,902,758 + IAI47_13710 0.10 -1.6 2,902,759 - IAI47_13710 0.11 +2.9 2,902,759 - IAI47_13710 0.11 -1.1 2,902,759 - IAI47_13710 0.11 -1.3 2,902,759 - IAI47_13710 0.11 -1.7 2,902,759 - IAI47_13710 0.11 -0.4 2,902,784 + IAI47_13710 0.19 +1.5 2,902,826 + IAI47_13710 0.33 -0.1 2,902,827 - IAI47_13710 0.34 -0.1 2,902,972 - IAI47_13710 0.83 -0.2 2,902,996 + -0.7 2,902,996 + -1.7 2,902,996 + -1.2 2,902,996 + +0.0 2,902,996 + +0.0 2,902,996 + +0.1 2,902,997 - -1.4 2,902,997 - +0.3 2,902,997 - +1.8 2,903,015 + +1.0 2,903,015 + -1.5 2,903,016 - +0.6 2,903,016 - -2.2 2,903,016 - -1.3 2,903,074 + IAI47_13715 0.50 -1.7 2,903,139 - -2.4 2,903,139 - -2.5 2,903,143 - -0.1 2,903,143 - +1.4 2,903,143 - -0.7
Or see this region's nucleotide sequence