Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_08755 and IAI47_08760 are separated by 91 nucleotides IAI47_08760 and IAI47_08765 are separated by 15 nucleotides IAI47_08765 and IAI47_08770 are separated by 63 nucleotides
IAI47_08755: IAI47_08755 - DUF986 domain-containing protein, at 1,850,171 to 1,850,629
_08755
IAI47_08760: IAI47_08760 - PTS mannose transporter subunit IID, at 1,850,721 to 1,851,566
_08760
IAI47_08765: IAI47_08765 - PTS mannose/fructose/sorbose transporter subunit IIC, at 1,851,582 to 1,852,382
_08765
IAI47_08770: IAI47_08770 - PTS mannose transporter subunit IIAB, at 1,852,446 to 1,853,408
_08770
Position (kb)
1851
1852
1853 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1850.596 kb on + strand at 1850.597 kb on - strand at 1850.597 kb on - strand at 1850.597 kb on - strand at 1850.597 kb on - strand at 1850.597 kb on - strand at 1850.621 kb on + strand at 1850.621 kb on + strand at 1850.636 kb on + strand at 1850.636 kb on + strand at 1850.637 kb on - strand at 1850.637 kb on - strand at 1850.686 kb on - strand at 1850.746 kb on + strand at 1850.757 kb on - strand at 1850.801 kb on + strand at 1850.801 kb on + strand at 1850.819 kb on + strand, within IAI47_08760 at 1850.819 kb on + strand, within IAI47_08760 at 1850.819 kb on + strand, within IAI47_08760 at 1850.819 kb on + strand, within IAI47_08760 at 1850.819 kb on + strand, within IAI47_08760 at 1850.819 kb on + strand, within IAI47_08760 at 1850.819 kb on + strand, within IAI47_08760 at 1850.819 kb on + strand, within IAI47_08760 at 1850.820 kb on - strand, within IAI47_08760 at 1850.820 kb on - strand, within IAI47_08760 at 1850.820 kb on - strand, within IAI47_08760 at 1850.820 kb on - strand, within IAI47_08760 at 1850.901 kb on - strand, within IAI47_08760 at 1850.943 kb on - strand, within IAI47_08760 at 1851.059 kb on + strand, within IAI47_08760 at 1851.059 kb on + strand, within IAI47_08760 at 1851.070 kb on + strand, within IAI47_08760 at 1851.071 kb on - strand, within IAI47_08760 at 1851.071 kb on - strand, within IAI47_08760 at 1851.071 kb on - strand, within IAI47_08760 at 1851.083 kb on - strand, within IAI47_08760 at 1851.083 kb on - strand, within IAI47_08760 at 1851.085 kb on + strand, within IAI47_08760 at 1851.111 kb on - strand, within IAI47_08760 at 1851.193 kb on - strand, within IAI47_08760 at 1851.193 kb on - strand, within IAI47_08760 at 1851.193 kb on - strand, within IAI47_08760 at 1851.343 kb on + strand, within IAI47_08760 at 1851.343 kb on + strand, within IAI47_08760 at 1851.343 kb on + strand, within IAI47_08760 at 1851.343 kb on + strand, within IAI47_08760 at 1851.344 kb on - strand, within IAI47_08760 at 1851.344 kb on - strand, within IAI47_08760 at 1851.344 kb on - strand, within IAI47_08760 at 1851.344 kb on - strand, within IAI47_08760 at 1851.344 kb on - strand, within IAI47_08760 at 1851.344 kb on - strand, within IAI47_08760 at 1851.344 kb on - strand, within IAI47_08760 at 1851.356 kb on + strand, within IAI47_08760 at 1851.378 kb on + strand, within IAI47_08760 at 1851.378 kb on + strand, within IAI47_08760 at 1851.379 kb on - strand, within IAI47_08760 at 1851.389 kb on + strand, within IAI47_08760 at 1851.389 kb on + strand, within IAI47_08760 at 1851.389 kb on + strand, within IAI47_08760 at 1851.389 kb on + strand, within IAI47_08760 at 1851.390 kb on - strand, within IAI47_08760 at 1851.437 kb on + strand, within IAI47_08760 at 1851.437 kb on + strand, within IAI47_08760 at 1851.437 kb on + strand, within IAI47_08760 at 1851.437 kb on + strand, within IAI47_08760 at 1851.438 kb on - strand, within IAI47_08760 at 1851.438 kb on - strand, within IAI47_08760 at 1851.438 kb on - strand, within IAI47_08760 at 1851.464 kb on + strand, within IAI47_08760 at 1851.464 kb on + strand, within IAI47_08760 at 1851.464 kb on + strand, within IAI47_08760 at 1851.464 kb on + strand, within IAI47_08760 at 1851.465 kb on - strand, within IAI47_08760 at 1851.465 kb on - strand, within IAI47_08760 at 1851.465 kb on - strand, within IAI47_08760 at 1851.465 kb on - strand, within IAI47_08760 at 1851.506 kb on + strand at 1851.554 kb on + strand at 1851.555 kb on - strand at 1851.555 kb on - strand at 1851.558 kb on - strand at 1851.558 kb on - strand at 1851.639 kb on + strand at 1851.639 kb on + strand at 1851.640 kb on - strand at 1851.649 kb on + strand at 1851.650 kb on - strand at 1851.670 kb on + strand, within IAI47_08765 at 1851.670 kb on + strand, within IAI47_08765 at 1851.670 kb on + strand, within IAI47_08765 at 1851.670 kb on + strand, within IAI47_08765 at 1851.670 kb on + strand, within IAI47_08765 at 1851.671 kb on - strand, within IAI47_08765 at 1851.696 kb on + strand, within IAI47_08765 at 1851.696 kb on + strand, within IAI47_08765 at 1851.697 kb on - strand, within IAI47_08765 at 1851.726 kb on - strand, within IAI47_08765 at 1851.752 kb on - strand, within IAI47_08765 at 1851.752 kb on - strand, within IAI47_08765 at 1851.769 kb on + strand, within IAI47_08765 at 1851.769 kb on + strand, within IAI47_08765 at 1851.833 kb on + strand, within IAI47_08765 at 1851.863 kb on + strand, within IAI47_08765 at 1851.864 kb on - strand, within IAI47_08765 at 1851.864 kb on - strand, within IAI47_08765 at 1851.926 kb on + strand, within IAI47_08765 at 1851.932 kb on + strand, within IAI47_08765 at 1851.933 kb on - strand, within IAI47_08765 at 1851.989 kb on + strand, within IAI47_08765 at 1851.990 kb on - strand, within IAI47_08765 at 1852.007 kb on + strand, within IAI47_08765 at 1852.145 kb on + strand, within IAI47_08765 at 1852.145 kb on + strand, within IAI47_08765 at 1852.232 kb on + strand, within IAI47_08765 at 1852.232 kb on + strand, within IAI47_08765 at 1852.232 kb on + strand, within IAI47_08765 at 1852.232 kb on + strand, within IAI47_08765 at 1852.247 kb on + strand, within IAI47_08765 at 1852.274 kb on + strand, within IAI47_08765 at 1852.274 kb on + strand, within IAI47_08765 at 1852.275 kb on - strand, within IAI47_08765 at 1852.275 kb on - strand, within IAI47_08765 at 1852.275 kb on - strand, within IAI47_08765 at 1852.275 kb on - strand, within IAI47_08765 at 1852.375 kb on - strand at 1852.385 kb on + strand at 1852.385 kb on + strand at 1852.386 kb on - strand at 1852.386 kb on - strand at 1852.386 kb on - strand at 1852.386 kb on - strand at 1852.386 kb on - strand at 1852.386 kb on - strand at 1852.454 kb on + strand at 1852.523 kb on + strand at 1852.524 kb on - strand at 1852.524 kb on - strand at 1852.556 kb on + strand, within IAI47_08770 at 1852.557 kb on - strand, within IAI47_08770 at 1852.583 kb on + strand, within IAI47_08770 at 1852.583 kb on + strand, within IAI47_08770 at 1852.583 kb on + strand, within IAI47_08770 at 1852.584 kb on - strand, within IAI47_08770 at 1852.584 kb on - strand, within IAI47_08770 at 1852.584 kb on - strand, within IAI47_08770 at 1852.638 kb on - strand, within IAI47_08770 at 1852.638 kb on - strand, within IAI47_08770 at 1852.640 kb on + strand, within IAI47_08770 at 1852.686 kb on - strand, within IAI47_08770 at 1852.686 kb on - strand, within IAI47_08770 at 1852.714 kb on + strand, within IAI47_08770 at 1852.714 kb on + strand, within IAI47_08770 at 1852.714 kb on + strand, within IAI47_08770 at 1852.714 kb on + strand, within IAI47_08770 at 1852.715 kb on - strand, within IAI47_08770 at 1852.715 kb on - strand, within IAI47_08770 at 1852.715 kb on - strand, within IAI47_08770 at 1852.715 kb on - strand, within IAI47_08770 at 1852.725 kb on - strand, within IAI47_08770 at 1852.745 kb on + strand, within IAI47_08770 at 1852.805 kb on + strand, within IAI47_08770 at 1852.805 kb on + strand, within IAI47_08770 at 1852.806 kb on - strand, within IAI47_08770 at 1852.806 kb on - strand, within IAI47_08770 at 1852.806 kb on - strand, within IAI47_08770 at 1852.845 kb on - strand, within IAI47_08770 at 1852.911 kb on + strand, within IAI47_08770 at 1852.911 kb on + strand, within IAI47_08770 at 1852.911 kb on + strand, within IAI47_08770 at 1852.911 kb on + strand, within IAI47_08770 at 1852.911 kb on + strand, within IAI47_08770 at 1852.911 kb on + strand, within IAI47_08770 at 1852.911 kb on + strand, within IAI47_08770 at 1852.912 kb on - strand, within IAI47_08770 at 1852.912 kb on - strand, within IAI47_08770 at 1852.912 kb on - strand, within IAI47_08770 at 1852.912 kb on - strand, within IAI47_08770 at 1852.912 kb on - strand, within IAI47_08770 at 1852.912 kb on - strand, within IAI47_08770 at 1852.986 kb on + strand, within IAI47_08770 at 1853.030 kb on + strand, within IAI47_08770 at 1853.031 kb on - strand, within IAI47_08770 at 1853.126 kb on + strand, within IAI47_08770 at 1853.149 kb on + strand, within IAI47_08770 at 1853.149 kb on + strand, within IAI47_08770 at 1853.149 kb on + strand, within IAI47_08770 at 1853.149 kb on + strand, within IAI47_08770 at 1853.150 kb on - strand, within IAI47_08770 at 1853.150 kb on - strand, within IAI47_08770 at 1853.160 kb on - strand, within IAI47_08770 at 1853.171 kb on + strand, within IAI47_08770 at 1853.187 kb on - strand, within IAI47_08770 at 1853.207 kb on + strand, within IAI47_08770 at 1853.208 kb on - strand, within IAI47_08770 at 1853.237 kb on + strand, within IAI47_08770 at 1853.237 kb on + strand, within IAI47_08770 at 1853.237 kb on + strand, within IAI47_08770 at 1853.261 kb on + strand, within IAI47_08770 at 1853.261 kb on + strand, within IAI47_08770 at 1853.261 kb on + strand, within IAI47_08770 at 1853.262 kb on - strand, within IAI47_08770 at 1853.262 kb on - strand, within IAI47_08770 at 1853.262 kb on - strand, within IAI47_08770 at 1853.262 kb on - strand, within IAI47_08770 at 1853.263 kb on + strand, within IAI47_08770 at 1853.264 kb on - strand, within IAI47_08770 at 1853.264 kb on - strand, within IAI47_08770
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,850,596 + -0.5 1,850,597 - +1.3 1,850,597 - +1.1 1,850,597 - -0.4 1,850,597 - +1.5 1,850,597 - -0.9 1,850,621 + -1.5 1,850,621 + -1.6 1,850,636 + -0.1 1,850,636 + +0.1 1,850,637 - +0.7 1,850,637 - -1.3 1,850,686 - -1.9 1,850,746 + +0.3 1,850,757 - -0.9 1,850,801 + +0.5 1,850,801 + -0.6 1,850,819 + IAI47_08760 0.12 +1.0 1,850,819 + IAI47_08760 0.12 +0.4 1,850,819 + IAI47_08760 0.12 +2.4 1,850,819 + IAI47_08760 0.12 -2.4 1,850,819 + IAI47_08760 0.12 +2.0 1,850,819 + IAI47_08760 0.12 +0.7 1,850,819 + IAI47_08760 0.12 -0.2 1,850,819 + IAI47_08760 0.12 -1.3 1,850,820 - IAI47_08760 0.12 -0.3 1,850,820 - IAI47_08760 0.12 +1.5 1,850,820 - IAI47_08760 0.12 -3.0 1,850,820 - IAI47_08760 0.12 +1.0 1,850,901 - IAI47_08760 0.21 -2.5 1,850,943 - IAI47_08760 0.26 -0.8 1,851,059 + IAI47_08760 0.40 -2.3 1,851,059 + IAI47_08760 0.40 +0.4 1,851,070 + IAI47_08760 0.41 -0.9 1,851,071 - IAI47_08760 0.41 +1.0 1,851,071 - IAI47_08760 0.41 -0.6 1,851,071 - IAI47_08760 0.41 +0.7 1,851,083 - IAI47_08760 0.43 -1.5 1,851,083 - IAI47_08760 0.43 +2.0 1,851,085 + IAI47_08760 0.43 +0.8 1,851,111 - IAI47_08760 0.46 +0.1 1,851,193 - IAI47_08760 0.56 -1.8 1,851,193 - IAI47_08760 0.56 +4.0 1,851,193 - IAI47_08760 0.56 -0.3 1,851,343 + IAI47_08760 0.74 -1.7 1,851,343 + IAI47_08760 0.74 -0.1 1,851,343 + IAI47_08760 0.74 +0.2 1,851,343 + IAI47_08760 0.74 +0.8 1,851,344 - IAI47_08760 0.74 +3.6 1,851,344 - IAI47_08760 0.74 -1.0 1,851,344 - IAI47_08760 0.74 +1.9 1,851,344 - IAI47_08760 0.74 -0.6 1,851,344 - IAI47_08760 0.74 -1.6 1,851,344 - IAI47_08760 0.74 +2.7 1,851,344 - IAI47_08760 0.74 -0.8 1,851,356 + IAI47_08760 0.75 +2.7 1,851,378 + IAI47_08760 0.78 +0.5 1,851,378 + IAI47_08760 0.78 +1.4 1,851,379 - IAI47_08760 0.78 -0.1 1,851,389 + IAI47_08760 0.79 +2.6 1,851,389 + IAI47_08760 0.79 +3.7 1,851,389 + IAI47_08760 0.79 -1.9 1,851,389 + IAI47_08760 0.79 +0.5 1,851,390 - IAI47_08760 0.79 -0.8 1,851,437 + IAI47_08760 0.85 -2.3 1,851,437 + IAI47_08760 0.85 +1.3 1,851,437 + IAI47_08760 0.85 -0.5 1,851,437 + IAI47_08760 0.85 +0.8 1,851,438 - IAI47_08760 0.85 +1.4 1,851,438 - IAI47_08760 0.85 +0.9 1,851,438 - IAI47_08760 0.85 +1.6 1,851,464 + IAI47_08760 0.88 -1.6 1,851,464 + IAI47_08760 0.88 +1.2 1,851,464 + IAI47_08760 0.88 +0.6 1,851,464 + IAI47_08760 0.88 -0.2 1,851,465 - IAI47_08760 0.88 -0.8 1,851,465 - IAI47_08760 0.88 +1.8 1,851,465 - IAI47_08760 0.88 +1.1 1,851,465 - IAI47_08760 0.88 +1.5 1,851,506 + -0.6 1,851,554 + +0.3 1,851,555 - -0.6 1,851,555 - -1.1 1,851,558 - -0.3 1,851,558 - -0.4 1,851,639 + +0.7 1,851,639 + -1.7 1,851,640 - +0.7 1,851,649 + -2.3 1,851,650 - +0.1 1,851,670 + IAI47_08765 0.11 -1.0 1,851,670 + IAI47_08765 0.11 +0.5 1,851,670 + IAI47_08765 0.11 -0.2 1,851,670 + IAI47_08765 0.11 -1.0 1,851,670 + IAI47_08765 0.11 +0.4 1,851,671 - IAI47_08765 0.11 +2.7 1,851,696 + IAI47_08765 0.14 +0.3 1,851,696 + IAI47_08765 0.14 +1.3 1,851,697 - IAI47_08765 0.14 +3.8 1,851,726 - IAI47_08765 0.18 +1.5 1,851,752 - IAI47_08765 0.21 +1.4 1,851,752 - IAI47_08765 0.21 -1.0 1,851,769 + IAI47_08765 0.23 +1.0 1,851,769 + IAI47_08765 0.23 -0.5 1,851,833 + IAI47_08765 0.31 -0.6 1,851,863 + IAI47_08765 0.35 +1.0 1,851,864 - IAI47_08765 0.35 +0.0 1,851,864 - IAI47_08765 0.35 +0.5 1,851,926 + IAI47_08765 0.43 -2.0 1,851,932 + IAI47_08765 0.44 -1.1 1,851,933 - IAI47_08765 0.44 -1.8 1,851,989 + IAI47_08765 0.51 -2.2 1,851,990 - IAI47_08765 0.51 +1.0 1,852,007 + IAI47_08765 0.53 +1.8 1,852,145 + IAI47_08765 0.70 +1.4 1,852,145 + IAI47_08765 0.70 +1.4 1,852,232 + IAI47_08765 0.81 -0.1 1,852,232 + IAI47_08765 0.81 +0.3 1,852,232 + IAI47_08765 0.81 +0.3 1,852,232 + IAI47_08765 0.81 +1.7 1,852,247 + IAI47_08765 0.83 +0.1 1,852,274 + IAI47_08765 0.86 +0.5 1,852,274 + IAI47_08765 0.86 -0.3 1,852,275 - IAI47_08765 0.87 +0.9 1,852,275 - IAI47_08765 0.87 -0.2 1,852,275 - IAI47_08765 0.87 -1.0 1,852,275 - IAI47_08765 0.87 -1.4 1,852,375 - +1.0 1,852,385 + +0.1 1,852,385 + +0.7 1,852,386 - +0.3 1,852,386 - +0.3 1,852,386 - +0.8 1,852,386 - -0.5 1,852,386 - +0.5 1,852,386 - -0.7 1,852,454 + -0.9 1,852,523 + +1.3 1,852,524 - -0.7 1,852,524 - +0.4 1,852,556 + IAI47_08770 0.11 +1.0 1,852,557 - IAI47_08770 0.12 -1.7 1,852,583 + IAI47_08770 0.14 -1.5 1,852,583 + IAI47_08770 0.14 +0.7 1,852,583 + IAI47_08770 0.14 -1.7 1,852,584 - IAI47_08770 0.14 -1.1 1,852,584 - IAI47_08770 0.14 -0.6 1,852,584 - IAI47_08770 0.14 +1.2 1,852,638 - IAI47_08770 0.20 -0.3 1,852,638 - IAI47_08770 0.20 -1.4 1,852,640 + IAI47_08770 0.20 -0.3 1,852,686 - IAI47_08770 0.25 +1.3 1,852,686 - IAI47_08770 0.25 +1.8 1,852,714 + IAI47_08770 0.28 -0.4 1,852,714 + IAI47_08770 0.28 +1.8 1,852,714 + IAI47_08770 0.28 +2.1 1,852,714 + IAI47_08770 0.28 -0.9 1,852,715 - IAI47_08770 0.28 +0.1 1,852,715 - IAI47_08770 0.28 -1.6 1,852,715 - IAI47_08770 0.28 +0.9 1,852,715 - IAI47_08770 0.28 -1.5 1,852,725 - IAI47_08770 0.29 +0.8 1,852,745 + IAI47_08770 0.31 +0.5 1,852,805 + IAI47_08770 0.37 +1.1 1,852,805 + IAI47_08770 0.37 -0.7 1,852,806 - IAI47_08770 0.37 +1.5 1,852,806 - IAI47_08770 0.37 -0.4 1,852,806 - IAI47_08770 0.37 -1.0 1,852,845 - IAI47_08770 0.41 -0.9 1,852,911 + IAI47_08770 0.48 -1.8 1,852,911 + IAI47_08770 0.48 +1.2 1,852,911 + IAI47_08770 0.48 +1.7 1,852,911 + IAI47_08770 0.48 +1.6 1,852,911 + IAI47_08770 0.48 +1.4 1,852,911 + IAI47_08770 0.48 +0.8 1,852,911 + IAI47_08770 0.48 +1.2 1,852,912 - IAI47_08770 0.48 +1.0 1,852,912 - IAI47_08770 0.48 +2.6 1,852,912 - IAI47_08770 0.48 -0.3 1,852,912 - IAI47_08770 0.48 +0.4 1,852,912 - IAI47_08770 0.48 -0.3 1,852,912 - IAI47_08770 0.48 +0.7 1,852,986 + IAI47_08770 0.56 +0.7 1,853,030 + IAI47_08770 0.61 +1.7 1,853,031 - IAI47_08770 0.61 +2.2 1,853,126 + IAI47_08770 0.71 +1.5 1,853,149 + IAI47_08770 0.73 -1.6 1,853,149 + IAI47_08770 0.73 -0.4 1,853,149 + IAI47_08770 0.73 -0.9 1,853,149 + IAI47_08770 0.73 -0.7 1,853,150 - IAI47_08770 0.73 +0.6 1,853,150 - IAI47_08770 0.73 -0.7 1,853,160 - IAI47_08770 0.74 +2.4 1,853,171 + IAI47_08770 0.75 -0.5 1,853,187 - IAI47_08770 0.77 +1.1 1,853,207 + IAI47_08770 0.79 +0.9 1,853,208 - IAI47_08770 0.79 +2.1 1,853,237 + IAI47_08770 0.82 -2.0 1,853,237 + IAI47_08770 0.82 -0.1 1,853,237 + IAI47_08770 0.82 +0.2 1,853,261 + IAI47_08770 0.85 +4.6 1,853,261 + IAI47_08770 0.85 +0.3 1,853,261 + IAI47_08770 0.85 -0.3 1,853,262 - IAI47_08770 0.85 -1.6 1,853,262 - IAI47_08770 0.85 +0.7 1,853,262 - IAI47_08770 0.85 +2.3 1,853,262 - IAI47_08770 0.85 +1.7 1,853,263 + IAI47_08770 0.85 +0.7 1,853,264 - IAI47_08770 0.85 +0.7 1,853,264 - IAI47_08770 0.85 +0.6
Or see this region's nucleotide sequence