Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_08495 and IAI47_08500 are separated by 214 nucleotides IAI47_08500 and IAI47_08505 are separated by 34 nucleotides
IAI47_08495: IAI47_08495 - glucose-6-phosphate dehydrogenase, at 1,798,855 to 1,800,330
_08495
IAI47_08500: IAI47_08500 - bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase, at 1,800,545 to 1,801,183
_08500
IAI47_08505: IAI47_08505 - formate-dependent phosphoribosylglycinamide formyltransferase, at 1,801,218 to 1,802,396
_08505
Position (kb)
1800
1801
1802 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1800.329 kb on - strand at 1800.332 kb on + strand at 1800.332 kb on + strand at 1800.332 kb on + strand at 1800.332 kb on + strand at 1800.332 kb on + strand at 1800.332 kb on + strand at 1800.332 kb on + strand at 1800.333 kb on - strand at 1800.333 kb on - strand at 1800.333 kb on - strand at 1800.333 kb on - strand at 1800.333 kb on - strand at 1800.333 kb on - strand at 1800.343 kb on + strand at 1800.343 kb on + strand at 1800.343 kb on + strand at 1800.344 kb on - strand at 1800.344 kb on - strand at 1800.376 kb on + strand at 1800.400 kb on - strand at 1800.437 kb on + strand at 1800.461 kb on + strand at 1800.461 kb on + strand at 1800.461 kb on + strand at 1800.461 kb on + strand at 1800.461 kb on + strand at 1800.461 kb on + strand at 1800.461 kb on + strand at 1800.461 kb on + strand at 1800.462 kb on - strand at 1800.462 kb on - strand at 1800.462 kb on - strand at 1800.462 kb on - strand at 1800.462 kb on - strand at 1800.462 kb on - strand at 1800.462 kb on - strand at 1800.467 kb on - strand at 1800.479 kb on + strand at 1800.514 kb on + strand at 1800.515 kb on - strand at 1800.526 kb on + strand at 1800.527 kb on - strand at 1800.765 kb on + strand, within IAI47_08500 at 1800.765 kb on + strand, within IAI47_08500 at 1800.765 kb on + strand, within IAI47_08500 at 1800.765 kb on + strand, within IAI47_08500 at 1800.769 kb on + strand, within IAI47_08500 at 1800.783 kb on + strand, within IAI47_08500 at 1800.818 kb on - strand, within IAI47_08500 at 1800.906 kb on + strand, within IAI47_08500 at 1800.923 kb on + strand, within IAI47_08500 at 1800.923 kb on + strand, within IAI47_08500 at 1800.924 kb on - strand, within IAI47_08500 at 1800.924 kb on - strand, within IAI47_08500 at 1800.924 kb on - strand, within IAI47_08500 at 1801.049 kb on + strand, within IAI47_08500 at 1801.049 kb on + strand, within IAI47_08500 at 1801.049 kb on + strand, within IAI47_08500 at 1801.049 kb on + strand, within IAI47_08500 at 1801.050 kb on - strand, within IAI47_08500 at 1801.050 kb on - strand, within IAI47_08500 at 1801.050 kb on - strand, within IAI47_08500 at 1801.050 kb on - strand, within IAI47_08500 at 1801.050 kb on - strand, within IAI47_08500 at 1801.050 kb on - strand, within IAI47_08500 at 1801.050 kb on - strand, within IAI47_08500 at 1801.050 kb on - strand, within IAI47_08500 at 1801.059 kb on - strand, within IAI47_08500 at 1801.059 kb on - strand, within IAI47_08500 at 1801.181 kb on + strand at 1801.181 kb on + strand at 1801.181 kb on + strand at 1801.181 kb on + strand at 1801.181 kb on + strand at 1801.181 kb on + strand at 1801.181 kb on + strand at 1801.181 kb on + strand at 1801.182 kb on - strand at 1801.182 kb on - strand at 1801.182 kb on - strand at 1801.182 kb on - strand at 1801.182 kb on - strand at 1801.182 kb on - strand at 1801.185 kb on + strand at 1801.185 kb on + strand at 1801.185 kb on + strand at 1801.185 kb on + strand at 1801.185 kb on + strand at 1801.186 kb on - strand at 1801.186 kb on - strand at 1801.186 kb on - strand at 1801.186 kb on - strand at 1801.213 kb on + strand at 1801.214 kb on - strand at 1801.280 kb on + strand at 1801.393 kb on - strand, within IAI47_08505 at 1801.444 kb on + strand, within IAI47_08505 at 1801.444 kb on + strand, within IAI47_08505 at 1801.444 kb on + strand, within IAI47_08505 at 1801.444 kb on + strand, within IAI47_08505 at 1801.444 kb on + strand, within IAI47_08505 at 1801.444 kb on + strand, within IAI47_08505 at 1801.444 kb on + strand, within IAI47_08505 at 1801.444 kb on + strand, within IAI47_08505 at 1801.445 kb on - strand, within IAI47_08505 at 1801.445 kb on - strand, within IAI47_08505 at 1801.557 kb on + strand, within IAI47_08505 at 1801.557 kb on + strand, within IAI47_08505 at 1801.557 kb on + strand, within IAI47_08505 at 1801.558 kb on - strand, within IAI47_08505 at 1801.616 kb on - strand, within IAI47_08505 at 1801.616 kb on - strand, within IAI47_08505 at 1801.632 kb on - strand, within IAI47_08505 at 1801.772 kb on + strand, within IAI47_08505 at 1801.772 kb on + strand, within IAI47_08505 at 1801.772 kb on + strand, within IAI47_08505 at 1801.773 kb on - strand, within IAI47_08505 at 1801.773 kb on - strand, within IAI47_08505 at 1801.858 kb on + strand, within IAI47_08505 at 1801.858 kb on + strand, within IAI47_08505 at 1801.858 kb on + strand, within IAI47_08505 at 1801.859 kb on - strand, within IAI47_08505 at 1801.910 kb on + strand, within IAI47_08505
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,800,329 - +1.2 1,800,332 + -0.9 1,800,332 + +2.5 1,800,332 + +1.0 1,800,332 + -1.2 1,800,332 + +1.8 1,800,332 + +1.4 1,800,332 + -1.0 1,800,333 - -0.9 1,800,333 - +0.1 1,800,333 - +0.0 1,800,333 - +1.1 1,800,333 - +1.1 1,800,333 - -1.5 1,800,343 + -0.9 1,800,343 + +1.3 1,800,343 + +0.3 1,800,344 - -2.1 1,800,344 - -1.2 1,800,376 + +0.1 1,800,400 - -1.4 1,800,437 + +0.7 1,800,461 + +3.1 1,800,461 + -0.3 1,800,461 + +0.3 1,800,461 + -1.2 1,800,461 + +0.6 1,800,461 + +0.2 1,800,461 + +0.5 1,800,461 + -0.7 1,800,462 - -1.4 1,800,462 - +0.5 1,800,462 - +0.8 1,800,462 - -2.3 1,800,462 - -2.3 1,800,462 - +0.7 1,800,462 - +1.3 1,800,467 - -2.0 1,800,479 + +0.6 1,800,514 + +0.9 1,800,515 - +0.0 1,800,526 + +0.8 1,800,527 - +2.4 1,800,765 + IAI47_08500 0.34 +0.5 1,800,765 + IAI47_08500 0.34 -2.0 1,800,765 + IAI47_08500 0.34 -0.2 1,800,765 + IAI47_08500 0.34 -1.2 1,800,769 + IAI47_08500 0.35 -0.5 1,800,783 + IAI47_08500 0.37 -1.7 1,800,818 - IAI47_08500 0.43 +0.5 1,800,906 + IAI47_08500 0.56 -0.2 1,800,923 + IAI47_08500 0.59 +1.6 1,800,923 + IAI47_08500 0.59 -0.6 1,800,924 - IAI47_08500 0.59 -1.0 1,800,924 - IAI47_08500 0.59 -1.6 1,800,924 - IAI47_08500 0.59 +0.4 1,801,049 + IAI47_08500 0.79 +0.5 1,801,049 + IAI47_08500 0.79 -0.3 1,801,049 + IAI47_08500 0.79 -1.7 1,801,049 + IAI47_08500 0.79 -0.6 1,801,050 - IAI47_08500 0.79 +0.7 1,801,050 - IAI47_08500 0.79 -0.6 1,801,050 - IAI47_08500 0.79 +1.3 1,801,050 - IAI47_08500 0.79 -1.4 1,801,050 - IAI47_08500 0.79 -1.1 1,801,050 - IAI47_08500 0.79 -0.0 1,801,050 - IAI47_08500 0.79 +0.1 1,801,050 - IAI47_08500 0.79 +0.7 1,801,059 - IAI47_08500 0.80 +1.5 1,801,059 - IAI47_08500 0.80 +0.5 1,801,181 + +0.0 1,801,181 + -1.8 1,801,181 + -1.0 1,801,181 + +0.9 1,801,181 + -0.0 1,801,181 + -1.0 1,801,181 + -0.1 1,801,181 + -1.8 1,801,182 - +0.0 1,801,182 - -2.2 1,801,182 - -1.9 1,801,182 - -0.8 1,801,182 - -1.5 1,801,182 - -1.6 1,801,185 + +2.5 1,801,185 + -0.6 1,801,185 + -1.1 1,801,185 + +1.7 1,801,185 + +1.0 1,801,186 - -0.8 1,801,186 - +0.5 1,801,186 - -1.1 1,801,186 - +0.9 1,801,213 + -1.2 1,801,214 - +0.0 1,801,280 + -0.4 1,801,393 - IAI47_08505 0.15 +0.2 1,801,444 + IAI47_08505 0.19 -1.8 1,801,444 + IAI47_08505 0.19 -0.7 1,801,444 + IAI47_08505 0.19 +1.0 1,801,444 + IAI47_08505 0.19 -1.6 1,801,444 + IAI47_08505 0.19 +0.1 1,801,444 + IAI47_08505 0.19 -0.7 1,801,444 + IAI47_08505 0.19 -1.1 1,801,444 + IAI47_08505 0.19 -1.9 1,801,445 - IAI47_08505 0.19 -0.9 1,801,445 - IAI47_08505 0.19 +0.7 1,801,557 + IAI47_08505 0.29 -0.7 1,801,557 + IAI47_08505 0.29 -0.6 1,801,557 + IAI47_08505 0.29 +0.1 1,801,558 - IAI47_08505 0.29 +0.2 1,801,616 - IAI47_08505 0.34 +0.3 1,801,616 - IAI47_08505 0.34 +1.1 1,801,632 - IAI47_08505 0.35 +1.1 1,801,772 + IAI47_08505 0.47 +0.3 1,801,772 + IAI47_08505 0.47 +0.3 1,801,772 + IAI47_08505 0.47 +0.2 1,801,773 - IAI47_08505 0.47 -1.4 1,801,773 - IAI47_08505 0.47 -0.0 1,801,858 + IAI47_08505 0.54 -1.0 1,801,858 + IAI47_08505 0.54 +0.1 1,801,858 + IAI47_08505 0.54 -0.1 1,801,859 - IAI47_08505 0.54 +0.2 1,801,910 + IAI47_08505 0.59 -0.7
Or see this region's nucleotide sequence