Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_07025 and IAI47_07030 are separated by 7 nucleotides IAI47_07030 and IAI47_07035 are separated by 97 nucleotides
IAI47_07025: IAI47_07025 - glycosyltransferase family 4 protein, at 1,482,495 to 1,483,685
_07025
IAI47_07030: IAI47_07030 - HAD family hydrolase, at 1,483,693 to 1,485,726
_07030
IAI47_07035: IAI47_07035 - NADP-dependent phosphogluconate dehydrogenase, at 1,485,824 to 1,487,233
_07035
Position (kb)
1483
1484
1485
1486 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1482.786 kb on + strand, within IAI47_07025 at 1482.787 kb on - strand, within IAI47_07025 at 1482.877 kb on + strand, within IAI47_07025 at 1482.903 kb on - strand, within IAI47_07025 at 1482.903 kb on - strand, within IAI47_07025 at 1482.966 kb on + strand, within IAI47_07025 at 1483.003 kb on + strand, within IAI47_07025 at 1483.004 kb on - strand, within IAI47_07025 at 1483.025 kb on + strand, within IAI47_07025 at 1483.079 kb on + strand, within IAI47_07025 at 1483.205 kb on - strand, within IAI47_07025 at 1483.217 kb on + strand, within IAI47_07025 at 1483.327 kb on + strand, within IAI47_07025 at 1483.328 kb on - strand, within IAI47_07025 at 1483.334 kb on + strand, within IAI47_07025 at 1483.368 kb on - strand, within IAI47_07025 at 1483.397 kb on + strand, within IAI47_07025 at 1483.398 kb on - strand, within IAI47_07025 at 1483.398 kb on - strand, within IAI47_07025 at 1483.420 kb on + strand, within IAI47_07025 at 1483.501 kb on + strand, within IAI47_07025 at 1483.530 kb on + strand, within IAI47_07025 at 1483.531 kb on - strand, within IAI47_07025 at 1483.571 kb on + strand at 1483.572 kb on - strand at 1483.589 kb on + strand at 1483.589 kb on + strand at 1483.591 kb on + strand at 1483.591 kb on + strand at 1483.591 kb on + strand at 1483.592 kb on - strand at 1483.592 kb on - strand at 1483.594 kb on + strand at 1483.594 kb on + strand at 1483.595 kb on - strand at 1483.676 kb on + strand at 1484.068 kb on - strand, within IAI47_07030 at 1484.188 kb on - strand, within IAI47_07030 at 1484.224 kb on + strand, within IAI47_07030 at 1484.269 kb on - strand, within IAI47_07030 at 1484.277 kb on + strand, within IAI47_07030 at 1484.294 kb on + strand, within IAI47_07030 at 1484.294 kb on + strand, within IAI47_07030 at 1484.495 kb on + strand, within IAI47_07030 at 1484.692 kb on - strand, within IAI47_07030 at 1484.709 kb on + strand, within IAI47_07030 at 1484.824 kb on + strand, within IAI47_07030 at 1484.827 kb on - strand, within IAI47_07030 at 1484.827 kb on - strand, within IAI47_07030 at 1484.831 kb on - strand, within IAI47_07030 at 1484.855 kb on + strand, within IAI47_07030 at 1484.856 kb on - strand, within IAI47_07030 at 1484.860 kb on + strand, within IAI47_07030 at 1484.962 kb on + strand, within IAI47_07030 at 1485.043 kb on - strand, within IAI47_07030 at 1485.053 kb on + strand, within IAI47_07030 at 1485.053 kb on + strand, within IAI47_07030 at 1485.085 kb on + strand, within IAI47_07030 at 1485.086 kb on - strand, within IAI47_07030 at 1485.123 kb on + strand, within IAI47_07030 at 1485.159 kb on + strand, within IAI47_07030 at 1485.236 kb on + strand, within IAI47_07030 at 1485.236 kb on + strand, within IAI47_07030 at 1485.237 kb on - strand, within IAI47_07030 at 1485.240 kb on - strand, within IAI47_07030 at 1485.394 kb on - strand, within IAI47_07030 at 1485.394 kb on - strand, within IAI47_07030 at 1485.465 kb on + strand, within IAI47_07030 at 1485.514 kb on + strand, within IAI47_07030 at 1485.515 kb on - strand, within IAI47_07030 at 1485.601 kb on - strand at 1485.604 kb on - strand at 1485.630 kb on + strand at 1485.630 kb on + strand at 1485.655 kb on - strand at 1485.680 kb on + strand at 1485.714 kb on + strand at 1485.724 kb on + strand at 1485.724 kb on + strand at 1485.724 kb on + strand at 1485.724 kb on + strand at 1485.725 kb on - strand at 1485.725 kb on - strand at 1485.728 kb on + strand at 1485.734 kb on - strand at 1485.792 kb on + strand at 1486.561 kb on + strand, within IAI47_07035
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,482,786 + IAI47_07025 0.24 -1.5 1,482,787 - IAI47_07025 0.25 -3.1 1,482,877 + IAI47_07025 0.32 -2.2 1,482,903 - IAI47_07025 0.34 -2.9 1,482,903 - IAI47_07025 0.34 -1.0 1,482,966 + IAI47_07025 0.40 -3.2 1,483,003 + IAI47_07025 0.43 +0.6 1,483,004 - IAI47_07025 0.43 -0.7 1,483,025 + IAI47_07025 0.45 -2.9 1,483,079 + IAI47_07025 0.49 -2.9 1,483,205 - IAI47_07025 0.60 -0.1 1,483,217 + IAI47_07025 0.61 -1.8 1,483,327 + IAI47_07025 0.70 -1.6 1,483,328 - IAI47_07025 0.70 -2.6 1,483,334 + IAI47_07025 0.70 -1.1 1,483,368 - IAI47_07025 0.73 -1.5 1,483,397 + IAI47_07025 0.76 -3.2 1,483,398 - IAI47_07025 0.76 -0.1 1,483,398 - IAI47_07025 0.76 -3.0 1,483,420 + IAI47_07025 0.78 -0.4 1,483,501 + IAI47_07025 0.84 -1.7 1,483,530 + IAI47_07025 0.87 -1.9 1,483,531 - IAI47_07025 0.87 -1.1 1,483,571 + -1.7 1,483,572 - -2.0 1,483,589 + -1.2 1,483,589 + -3.7 1,483,591 + -2.4 1,483,591 + -1.4 1,483,591 + +0.2 1,483,592 - -2.8 1,483,592 - -3.0 1,483,594 + -1.6 1,483,594 + +1.2 1,483,595 - -0.6 1,483,676 + -0.7 1,484,068 - IAI47_07030 0.18 -0.6 1,484,188 - IAI47_07030 0.24 -2.5 1,484,224 + IAI47_07030 0.26 -1.2 1,484,269 - IAI47_07030 0.28 -3.7 1,484,277 + IAI47_07030 0.29 -3.9 1,484,294 + IAI47_07030 0.30 -2.0 1,484,294 + IAI47_07030 0.30 -2.8 1,484,495 + IAI47_07030 0.39 -3.7 1,484,692 - IAI47_07030 0.49 -3.5 1,484,709 + IAI47_07030 0.50 -3.0 1,484,824 + IAI47_07030 0.56 -1.5 1,484,827 - IAI47_07030 0.56 -3.8 1,484,827 - IAI47_07030 0.56 -2.2 1,484,831 - IAI47_07030 0.56 -3.7 1,484,855 + IAI47_07030 0.57 -2.8 1,484,856 - IAI47_07030 0.57 -3.0 1,484,860 + IAI47_07030 0.57 -1.1 1,484,962 + IAI47_07030 0.62 +0.4 1,485,043 - IAI47_07030 0.66 -1.5 1,485,053 + IAI47_07030 0.67 -3.1 1,485,053 + IAI47_07030 0.67 -1.6 1,485,085 + IAI47_07030 0.68 -3.0 1,485,086 - IAI47_07030 0.68 -1.9 1,485,123 + IAI47_07030 0.70 +0.7 1,485,159 + IAI47_07030 0.72 -0.6 1,485,236 + IAI47_07030 0.76 -3.3 1,485,236 + IAI47_07030 0.76 -3.6 1,485,237 - IAI47_07030 0.76 +0.3 1,485,240 - IAI47_07030 0.76 -2.2 1,485,394 - IAI47_07030 0.84 +0.3 1,485,394 - IAI47_07030 0.84 -2.9 1,485,465 + IAI47_07030 0.87 -4.6 1,485,514 + IAI47_07030 0.90 -1.8 1,485,515 - IAI47_07030 0.90 -3.9 1,485,601 - -3.5 1,485,604 - -1.8 1,485,630 + -1.8 1,485,630 + -2.5 1,485,655 - +1.4 1,485,680 + -2.5 1,485,714 + -0.2 1,485,724 + -1.2 1,485,724 + +2.5 1,485,724 + -0.7 1,485,724 + +0.0 1,485,725 - +0.5 1,485,725 - -0.2 1,485,728 + -0.8 1,485,734 - -1.3 1,485,792 + -1.6 1,486,561 + IAI47_07035 0.52 -1.1
Or see this region's nucleotide sequence