Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_05285 and IAI47_05290 are separated by 97 nucleotides IAI47_05290 and IAI47_05295 are separated by 31 nucleotides IAI47_05295 and IAI47_05300 are separated by 22 nucleotides
IAI47_05285: IAI47_05285 - tRNA(Met) cytidine acetyltransferase, at 1,116,507 to 1,118,459
_05285
IAI47_05290: IAI47_05290 - esterase, at 1,118,557 to 1,119,237
_05290
IAI47_05295: IAI47_05295 - YpfN family protein, at 1,119,269 to 1,119,457
_05295
IAI47_05300: IAI47_05300 - succinyl-diaminopimelate desuccinylase, at 1,119,480 to 1,120,607
_05300
Position (kb)
1118
1119
1120 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1117.617 kb on + strand, within IAI47_05285 at 1117.664 kb on + strand, within IAI47_05285 at 1117.700 kb on - strand, within IAI47_05285 at 1117.700 kb on - strand, within IAI47_05285 at 1117.700 kb on - strand, within IAI47_05285 at 1117.700 kb on - strand, within IAI47_05285 at 1117.950 kb on + strand, within IAI47_05285 at 1117.950 kb on + strand, within IAI47_05285 at 1117.950 kb on + strand, within IAI47_05285 at 1117.950 kb on + strand, within IAI47_05285 at 1117.950 kb on + strand, within IAI47_05285 at 1117.951 kb on - strand, within IAI47_05285 at 1117.951 kb on - strand, within IAI47_05285 at 1117.951 kb on - strand, within IAI47_05285 at 1117.951 kb on - strand, within IAI47_05285 at 1117.951 kb on - strand, within IAI47_05285 at 1117.971 kb on + strand, within IAI47_05285 at 1118.012 kb on + strand, within IAI47_05285 at 1118.012 kb on + strand, within IAI47_05285 at 1118.026 kb on + strand, within IAI47_05285 at 1118.026 kb on + strand, within IAI47_05285 at 1118.027 kb on - strand, within IAI47_05285 at 1118.058 kb on + strand, within IAI47_05285 at 1118.058 kb on + strand, within IAI47_05285 at 1118.059 kb on - strand, within IAI47_05285 at 1118.059 kb on - strand, within IAI47_05285 at 1118.073 kb on - strand, within IAI47_05285 at 1118.073 kb on - strand, within IAI47_05285 at 1118.104 kb on + strand, within IAI47_05285 at 1118.159 kb on + strand, within IAI47_05285 at 1118.160 kb on - strand, within IAI47_05285 at 1118.160 kb on - strand, within IAI47_05285 at 1118.160 kb on - strand, within IAI47_05285 at 1118.160 kb on - strand, within IAI47_05285 at 1118.195 kb on + strand, within IAI47_05285 at 1118.195 kb on + strand, within IAI47_05285 at 1118.203 kb on + strand, within IAI47_05285 at 1118.203 kb on + strand, within IAI47_05285 at 1118.204 kb on - strand, within IAI47_05285 at 1118.204 kb on - strand, within IAI47_05285 at 1118.216 kb on + strand, within IAI47_05285 at 1118.216 kb on + strand, within IAI47_05285 at 1118.217 kb on - strand, within IAI47_05285 at 1118.217 kb on - strand, within IAI47_05285 at 1118.217 kb on - strand, within IAI47_05285 at 1118.217 kb on - strand, within IAI47_05285 at 1118.217 kb on - strand, within IAI47_05285 at 1118.318 kb on - strand at 1118.350 kb on + strand at 1118.387 kb on + strand at 1118.407 kb on + strand at 1118.407 kb on + strand at 1118.407 kb on + strand at 1118.408 kb on - strand at 1118.408 kb on - strand at 1118.408 kb on - strand at 1118.502 kb on - strand at 1118.532 kb on - strand at 1118.532 kb on - strand at 1118.569 kb on + strand at 1118.569 kb on + strand at 1118.569 kb on + strand at 1118.570 kb on - strand at 1118.570 kb on - strand at 1118.570 kb on - strand at 1118.570 kb on - strand at 1118.573 kb on + strand at 1118.573 kb on + strand at 1118.574 kb on - strand at 1118.574 kb on - strand at 1118.574 kb on - strand at 1118.593 kb on - strand at 1118.593 kb on - strand at 1118.593 kb on - strand at 1118.617 kb on + strand at 1118.617 kb on + strand at 1118.617 kb on + strand at 1118.763 kb on + strand, within IAI47_05290 at 1118.763 kb on + strand, within IAI47_05290 at 1118.763 kb on + strand, within IAI47_05290 at 1118.764 kb on - strand, within IAI47_05290 at 1118.858 kb on + strand, within IAI47_05290 at 1118.858 kb on + strand, within IAI47_05290 at 1118.858 kb on + strand, within IAI47_05290 at 1118.859 kb on - strand, within IAI47_05290 at 1118.883 kb on + strand, within IAI47_05290 at 1118.883 kb on + strand, within IAI47_05290 at 1118.884 kb on - strand, within IAI47_05290 at 1118.884 kb on - strand, within IAI47_05290 at 1118.884 kb on - strand, within IAI47_05290 at 1118.884 kb on - strand, within IAI47_05290 at 1118.952 kb on + strand, within IAI47_05290 at 1118.952 kb on + strand, within IAI47_05290 at 1119.010 kb on + strand, within IAI47_05290 at 1119.010 kb on + strand, within IAI47_05290 at 1119.010 kb on + strand, within IAI47_05290 at 1119.011 kb on - strand, within IAI47_05290 at 1119.011 kb on - strand, within IAI47_05290 at 1119.011 kb on - strand, within IAI47_05290 at 1119.159 kb on + strand, within IAI47_05290 at 1119.159 kb on + strand, within IAI47_05290 at 1119.159 kb on + strand, within IAI47_05290 at 1119.159 kb on + strand, within IAI47_05290 at 1119.159 kb on + strand, within IAI47_05290 at 1119.160 kb on - strand, within IAI47_05290 at 1119.160 kb on - strand, within IAI47_05290 at 1119.160 kb on - strand, within IAI47_05290 at 1119.160 kb on - strand, within IAI47_05290 at 1119.160 kb on - strand, within IAI47_05290 at 1119.160 kb on - strand, within IAI47_05290 at 1119.160 kb on - strand, within IAI47_05290 at 1119.160 kb on - strand, within IAI47_05290 at 1119.235 kb on + strand at 1119.235 kb on + strand at 1119.236 kb on - strand at 1119.271 kb on - strand at 1119.271 kb on - strand at 1119.271 kb on - strand at 1119.290 kb on - strand, within IAI47_05295 at 1119.307 kb on + strand, within IAI47_05295 at 1119.307 kb on + strand, within IAI47_05295 at 1119.307 kb on + strand, within IAI47_05295 at 1119.308 kb on - strand, within IAI47_05295 at 1119.308 kb on - strand, within IAI47_05295 at 1119.347 kb on - strand, within IAI47_05295 at 1119.349 kb on - strand, within IAI47_05295 at 1119.386 kb on - strand, within IAI47_05295 at 1119.387 kb on + strand, within IAI47_05295 at 1119.388 kb on - strand, within IAI47_05295 at 1119.388 kb on - strand, within IAI47_05295 at 1119.438 kb on + strand, within IAI47_05295 at 1119.439 kb on - strand at 1119.439 kb on - strand at 1119.449 kb on - strand at 1119.469 kb on + strand at 1119.470 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,117,617 + IAI47_05285 0.57 +2.2 1,117,664 + IAI47_05285 0.59 -0.4 1,117,700 - IAI47_05285 0.61 +2.9 1,117,700 - IAI47_05285 0.61 -0.9 1,117,700 - IAI47_05285 0.61 -1.4 1,117,700 - IAI47_05285 0.61 +0.4 1,117,950 + IAI47_05285 0.74 +1.2 1,117,950 + IAI47_05285 0.74 +1.4 1,117,950 + IAI47_05285 0.74 -1.8 1,117,950 + IAI47_05285 0.74 -0.9 1,117,950 + IAI47_05285 0.74 +1.1 1,117,951 - IAI47_05285 0.74 +0.7 1,117,951 - IAI47_05285 0.74 +0.2 1,117,951 - IAI47_05285 0.74 +0.9 1,117,951 - IAI47_05285 0.74 +1.8 1,117,951 - IAI47_05285 0.74 +0.7 1,117,971 + IAI47_05285 0.75 +0.2 1,118,012 + IAI47_05285 0.77 +0.4 1,118,012 + IAI47_05285 0.77 -3.1 1,118,026 + IAI47_05285 0.78 -1.9 1,118,026 + IAI47_05285 0.78 +3.6 1,118,027 - IAI47_05285 0.78 +0.5 1,118,058 + IAI47_05285 0.79 +1.6 1,118,058 + IAI47_05285 0.79 -0.6 1,118,059 - IAI47_05285 0.79 +0.2 1,118,059 - IAI47_05285 0.79 -0.3 1,118,073 - IAI47_05285 0.80 -1.2 1,118,073 - IAI47_05285 0.80 +0.7 1,118,104 + IAI47_05285 0.82 -1.3 1,118,159 + IAI47_05285 0.85 -0.2 1,118,160 - IAI47_05285 0.85 +0.4 1,118,160 - IAI47_05285 0.85 +0.5 1,118,160 - IAI47_05285 0.85 +0.1 1,118,160 - IAI47_05285 0.85 +0.5 1,118,195 + IAI47_05285 0.86 -1.6 1,118,195 + IAI47_05285 0.86 -0.2 1,118,203 + IAI47_05285 0.87 +1.4 1,118,203 + IAI47_05285 0.87 +1.3 1,118,204 - IAI47_05285 0.87 -0.5 1,118,204 - IAI47_05285 0.87 +0.4 1,118,216 + IAI47_05285 0.88 +1.8 1,118,216 + IAI47_05285 0.88 -1.4 1,118,217 - IAI47_05285 0.88 +0.3 1,118,217 - IAI47_05285 0.88 -2.0 1,118,217 - IAI47_05285 0.88 +0.3 1,118,217 - IAI47_05285 0.88 -1.7 1,118,217 - IAI47_05285 0.88 -2.9 1,118,318 - -2.4 1,118,350 + -1.6 1,118,387 + +1.4 1,118,407 + -0.3 1,118,407 + -0.7 1,118,407 + -1.5 1,118,408 - +0.8 1,118,408 - +0.0 1,118,408 - +1.6 1,118,502 - -0.4 1,118,532 - +0.5 1,118,532 - -1.0 1,118,569 + -1.4 1,118,569 + +0.2 1,118,569 + -0.1 1,118,570 - +1.1 1,118,570 - +0.4 1,118,570 - -1.3 1,118,570 - -2.6 1,118,573 + -1.7 1,118,573 + -0.7 1,118,574 - +0.8 1,118,574 - -2.2 1,118,574 - +0.6 1,118,593 - +0.9 1,118,593 - -2.4 1,118,593 - -0.8 1,118,617 + -0.2 1,118,617 + -0.8 1,118,617 + -0.8 1,118,763 + IAI47_05290 0.30 +2.2 1,118,763 + IAI47_05290 0.30 +1.6 1,118,763 + IAI47_05290 0.30 -1.4 1,118,764 - IAI47_05290 0.30 -0.5 1,118,858 + IAI47_05290 0.44 -1.6 1,118,858 + IAI47_05290 0.44 +0.2 1,118,858 + IAI47_05290 0.44 +0.3 1,118,859 - IAI47_05290 0.44 -1.3 1,118,883 + IAI47_05290 0.48 -1.9 1,118,883 + IAI47_05290 0.48 +1.5 1,118,884 - IAI47_05290 0.48 -0.2 1,118,884 - IAI47_05290 0.48 -1.7 1,118,884 - IAI47_05290 0.48 -0.3 1,118,884 - IAI47_05290 0.48 +2.3 1,118,952 + IAI47_05290 0.58 -0.1 1,118,952 + IAI47_05290 0.58 -2.3 1,119,010 + IAI47_05290 0.67 -2.9 1,119,010 + IAI47_05290 0.67 -0.1 1,119,010 + IAI47_05290 0.67 +0.5 1,119,011 - IAI47_05290 0.67 -0.0 1,119,011 - IAI47_05290 0.67 -2.7 1,119,011 - IAI47_05290 0.67 +0.8 1,119,159 + IAI47_05290 0.88 -0.3 1,119,159 + IAI47_05290 0.88 +0.4 1,119,159 + IAI47_05290 0.88 -1.7 1,119,159 + IAI47_05290 0.88 -1.9 1,119,159 + IAI47_05290 0.88 -0.4 1,119,160 - IAI47_05290 0.89 +1.4 1,119,160 - IAI47_05290 0.89 -1.8 1,119,160 - IAI47_05290 0.89 +1.7 1,119,160 - IAI47_05290 0.89 -2.1 1,119,160 - IAI47_05290 0.89 -0.2 1,119,160 - IAI47_05290 0.89 -1.0 1,119,160 - IAI47_05290 0.89 +0.9 1,119,160 - IAI47_05290 0.89 +0.5 1,119,235 + +0.7 1,119,235 + +0.3 1,119,236 - -1.5 1,119,271 - +0.4 1,119,271 - +0.7 1,119,271 - +0.6 1,119,290 - IAI47_05295 0.11 +0.6 1,119,307 + IAI47_05295 0.20 -0.1 1,119,307 + IAI47_05295 0.20 +0.3 1,119,307 + IAI47_05295 0.20 +1.0 1,119,308 - IAI47_05295 0.21 -1.0 1,119,308 - IAI47_05295 0.21 -0.1 1,119,347 - IAI47_05295 0.41 +2.5 1,119,349 - IAI47_05295 0.42 -1.6 1,119,386 - IAI47_05295 0.62 +2.3 1,119,387 + IAI47_05295 0.62 +0.8 1,119,388 - IAI47_05295 0.63 +0.3 1,119,388 - IAI47_05295 0.63 +0.7 1,119,438 + IAI47_05295 0.89 -1.1 1,119,439 - +1.6 1,119,439 - -0.1 1,119,449 - -1.7 1,119,469 + -0.7 1,119,470 - -1.6
Or see this region's nucleotide sequence