Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_02775 and IAI47_02780 are separated by 13 nucleotides IAI47_02780 and IAI47_02785 are separated by 2 nucleotides IAI47_02785 and IAI47_02790 are separated by 127 nucleotides IAI47_02790 and IAI47_02795 are separated by 11 nucleotides IAI47_02795 and IAI47_02800 are separated by 43 nucleotides
IAI47_02775: IAI47_02775 - glycine dehydrogenase, at 575,720 to 576,055
_02775
IAI47_02780: IAI47_02780 - hypothetical protein, at 576,069 to 576,425
_02780
IAI47_02785: IAI47_02785 - DUF4312 family protein, at 576,428 to 576,727
_02785
IAI47_02790: IAI47_02790 - DUF4311 domain-containing protein, at 576,855 to 577,631
_02790
IAI47_02795: IAI47_02795 - DUF4310 family protein, at 577,643 to 578,284
_02795
IAI47_02800: IAI47_02800 - amidohydrolase/deacetylase family metallohydrolase, at 578,328 to 579,458
_02800
Position (kb) 576
577
578 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 575.938 kb on + strand, within IAI47_02775 at 575.938 kb on + strand, within IAI47_02775 at 575.939 kb on - strand, within IAI47_02775 at 575.939 kb on - strand, within IAI47_02775 at 575.939 kb on - strand, within IAI47_02775 at 576.028 kb on + strand at 576.029 kb on - strand at 576.029 kb on - strand at 576.053 kb on + strand at 576.054 kb on - strand at 576.054 kb on - strand at 576.054 kb on - strand at 576.054 kb on - strand at 576.073 kb on + strand at 576.073 kb on + strand at 576.073 kb on + strand at 576.073 kb on + strand at 576.073 kb on + strand at 576.073 kb on + strand at 576.074 kb on - strand at 576.079 kb on + strand at 576.079 kb on + strand at 576.079 kb on + strand at 576.079 kb on + strand at 576.080 kb on - strand at 576.080 kb on - strand at 576.080 kb on - strand at 576.080 kb on - strand at 576.080 kb on - strand at 576.080 kb on - strand at 576.097 kb on + strand at 576.101 kb on + strand at 576.101 kb on + strand at 576.101 kb on + strand at 576.102 kb on - strand at 576.102 kb on - strand at 576.120 kb on - strand, within IAI47_02780 at 576.120 kb on - strand, within IAI47_02780 at 576.176 kb on + strand, within IAI47_02780 at 576.177 kb on - strand, within IAI47_02780 at 576.177 kb on - strand, within IAI47_02780 at 576.222 kb on - strand, within IAI47_02780 at 576.273 kb on + strand, within IAI47_02780 at 576.273 kb on + strand, within IAI47_02780 at 576.273 kb on + strand, within IAI47_02780 at 576.273 kb on + strand, within IAI47_02780 at 576.273 kb on + strand, within IAI47_02780 at 576.274 kb on - strand, within IAI47_02780 at 576.274 kb on - strand, within IAI47_02780 at 576.274 kb on - strand, within IAI47_02780 at 576.274 kb on - strand, within IAI47_02780 at 576.274 kb on - strand, within IAI47_02780 at 576.274 kb on - strand, within IAI47_02780 at 576.274 kb on - strand, within IAI47_02780 at 576.274 kb on - strand, within IAI47_02780 at 576.274 kb on - strand, within IAI47_02780 at 576.274 kb on - strand, within IAI47_02780 at 576.274 kb on - strand, within IAI47_02780 at 576.279 kb on + strand, within IAI47_02780 at 576.279 kb on + strand, within IAI47_02780 at 576.280 kb on - strand, within IAI47_02780 at 576.296 kb on + strand, within IAI47_02780 at 576.296 kb on + strand, within IAI47_02780 at 576.296 kb on + strand, within IAI47_02780 at 576.296 kb on + strand, within IAI47_02780 at 576.296 kb on + strand, within IAI47_02780 at 576.297 kb on - strand, within IAI47_02780 at 576.297 kb on - strand, within IAI47_02780 at 576.297 kb on - strand, within IAI47_02780 at 576.297 kb on - strand, within IAI47_02780 at 576.329 kb on + strand, within IAI47_02780 at 576.329 kb on + strand, within IAI47_02780 at 576.330 kb on - strand, within IAI47_02780 at 576.347 kb on - strand, within IAI47_02780 at 576.380 kb on - strand, within IAI47_02780 at 576.380 kb on - strand, within IAI47_02780 at 576.427 kb on + strand at 576.428 kb on - strand at 576.428 kb on - strand at 576.440 kb on + strand at 576.440 kb on + strand at 576.440 kb on + strand at 576.440 kb on + strand at 576.440 kb on + strand at 576.440 kb on + strand at 576.440 kb on + strand at 576.441 kb on - strand at 576.441 kb on - strand at 576.441 kb on - strand at 576.441 kb on - strand at 576.441 kb on - strand at 576.441 kb on - strand at 576.446 kb on - strand at 576.446 kb on - strand at 576.475 kb on + strand, within IAI47_02785 at 576.712 kb on + strand at 576.713 kb on - strand at 576.725 kb on + strand at 576.726 kb on - strand at 576.726 kb on - strand at 576.741 kb on + strand at 576.742 kb on - strand at 576.793 kb on - strand at 576.829 kb on + strand at 576.829 kb on + strand at 576.830 kb on - strand at 576.846 kb on + strand at 576.846 kb on + strand at 576.847 kb on - strand at 576.847 kb on - strand at 576.847 kb on - strand at 576.848 kb on - strand at 576.974 kb on + strand, within IAI47_02790 at 577.148 kb on + strand, within IAI47_02790 at 577.149 kb on - strand, within IAI47_02790 at 577.327 kb on + strand, within IAI47_02790 at 577.405 kb on + strand, within IAI47_02790 at 577.405 kb on + strand, within IAI47_02790 at 577.405 kb on + strand, within IAI47_02790 at 577.405 kb on + strand, within IAI47_02790 at 577.405 kb on + strand, within IAI47_02790 at 577.405 kb on + strand, within IAI47_02790 at 577.405 kb on + strand, within IAI47_02790 at 577.405 kb on + strand, within IAI47_02790 at 577.405 kb on + strand, within IAI47_02790 at 577.405 kb on + strand, within IAI47_02790 at 577.406 kb on - strand, within IAI47_02790 at 577.406 kb on - strand, within IAI47_02790 at 577.406 kb on - strand, within IAI47_02790 at 577.406 kb on - strand, within IAI47_02790 at 577.406 kb on - strand, within IAI47_02790 at 577.406 kb on - strand, within IAI47_02790 at 577.442 kb on + strand, within IAI47_02790 at 577.443 kb on - strand, within IAI47_02790 at 577.487 kb on + strand, within IAI47_02790 at 577.487 kb on + strand, within IAI47_02790 at 577.554 kb on - strand at 577.629 kb on + strand at 577.629 kb on + strand at 577.630 kb on - strand at 577.654 kb on + strand at 577.655 kb on - strand at 577.655 kb on - strand at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.736 kb on + strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.737 kb on - strand, within IAI47_02795 at 577.739 kb on + strand, within IAI47_02795 at 577.739 kb on + strand, within IAI47_02795 at 577.739 kb on + strand, within IAI47_02795 at 577.739 kb on + strand, within IAI47_02795 at 577.739 kb on + strand, within IAI47_02795 at 577.739 kb on + strand, within IAI47_02795 at 577.739 kb on + strand, within IAI47_02795 at 577.739 kb on + strand, within IAI47_02795 at 577.740 kb on - strand, within IAI47_02795 at 577.740 kb on - strand, within IAI47_02795 at 577.740 kb on - strand, within IAI47_02795 at 577.740 kb on - strand, within IAI47_02795 at 577.745 kb on + strand, within IAI47_02795 at 577.814 kb on + strand, within IAI47_02795 at 577.814 kb on + strand, within IAI47_02795 at 577.861 kb on + strand, within IAI47_02795 at 577.862 kb on - strand, within IAI47_02795 at 578.024 kb on + strand, within IAI47_02795 at 578.025 kb on - strand, within IAI47_02795 at 578.030 kb on - strand, within IAI47_02795 at 578.032 kb on + strand, within IAI47_02795 at 578.033 kb on - strand, within IAI47_02795 at 578.044 kb on + strand, within IAI47_02795 at 578.076 kb on - strand, within IAI47_02795 at 578.137 kb on + strand, within IAI47_02795 at 578.204 kb on + strand, within IAI47_02795 at 578.204 kb on + strand, within IAI47_02795 at 578.205 kb on - strand, within IAI47_02795 at 578.205 kb on - strand, within IAI47_02795 at 578.205 kb on - strand, within IAI47_02795 at 578.331 kb on + strand at 578.331 kb on + strand at 578.331 kb on + strand at 578.332 kb on - strand at 578.332 kb on - strand at 578.332 kb on - strand at 578.332 kb on - strand at 578.467 kb on - strand, within IAI47_02800 at 578.505 kb on + strand, within IAI47_02800 at 578.505 kb on + strand, within IAI47_02800 at 578.523 kb on + strand, within IAI47_02800 at 578.523 kb on + strand, within IAI47_02800 at 578.523 kb on + strand, within IAI47_02800 at 578.523 kb on + strand, within IAI47_02800 at 578.523 kb on + strand, within IAI47_02800 at 578.523 kb on + strand, within IAI47_02800 at 578.524 kb on - strand, within IAI47_02800 at 578.524 kb on - strand, within IAI47_02800 at 578.524 kb on - strand, within IAI47_02800 at 578.524 kb on - strand, within IAI47_02800 at 578.524 kb on - strand, within IAI47_02800 at 578.551 kb on + strand, within IAI47_02800 at 578.551 kb on + strand, within IAI47_02800 at 578.552 kb on - strand, within IAI47_02800 at 578.616 kb on + strand, within IAI47_02800
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 575,938 + IAI47_02775 0.65 -2.4 575,938 + IAI47_02775 0.65 +0.1 575,939 - IAI47_02775 0.65 +0.5 575,939 - IAI47_02775 0.65 -2.6 575,939 - IAI47_02775 0.65 -0.4 576,028 + +1.0 576,029 - -0.1 576,029 - -2.8 576,053 + -0.1 576,054 - +0.5 576,054 - -0.0 576,054 - +1.8 576,054 - +1.6 576,073 + +0.0 576,073 + +0.5 576,073 + +1.3 576,073 + -0.2 576,073 + +0.0 576,073 + -0.1 576,074 - +1.6 576,079 + +0.8 576,079 + +0.3 576,079 + -0.0 576,079 + -1.6 576,080 - +0.1 576,080 - -2.7 576,080 - +1.1 576,080 - -1.6 576,080 - +2.7 576,080 - -1.0 576,097 + -1.7 576,101 + +0.1 576,101 + +0.5 576,101 + -1.7 576,102 - -0.3 576,102 - +0.9 576,120 - IAI47_02780 0.14 +2.2 576,120 - IAI47_02780 0.14 +1.6 576,176 + IAI47_02780 0.30 -2.1 576,177 - IAI47_02780 0.30 +0.3 576,177 - IAI47_02780 0.30 +1.7 576,222 - IAI47_02780 0.43 +0.7 576,273 + IAI47_02780 0.57 +1.2 576,273 + IAI47_02780 0.57 -0.8 576,273 + IAI47_02780 0.57 +0.1 576,273 + IAI47_02780 0.57 -2.5 576,273 + IAI47_02780 0.57 -0.5 576,274 - IAI47_02780 0.57 -0.9 576,274 - IAI47_02780 0.57 +0.0 576,274 - IAI47_02780 0.57 +1.7 576,274 - IAI47_02780 0.57 +0.9 576,274 - IAI47_02780 0.57 +1.5 576,274 - IAI47_02780 0.57 -1.5 576,274 - IAI47_02780 0.57 +1.2 576,274 - IAI47_02780 0.57 -0.9 576,274 - IAI47_02780 0.57 -0.1 576,274 - IAI47_02780 0.57 -2.7 576,274 - IAI47_02780 0.57 -0.9 576,279 + IAI47_02780 0.59 +0.3 576,279 + IAI47_02780 0.59 -0.6 576,280 - IAI47_02780 0.59 +1.3 576,296 + IAI47_02780 0.64 -0.7 576,296 + IAI47_02780 0.64 +2.3 576,296 + IAI47_02780 0.64 -0.4 576,296 + IAI47_02780 0.64 -2.1 576,296 + IAI47_02780 0.64 -2.0 576,297 - IAI47_02780 0.64 +0.9 576,297 - IAI47_02780 0.64 +1.8 576,297 - IAI47_02780 0.64 +0.5 576,297 - IAI47_02780 0.64 -0.6 576,329 + IAI47_02780 0.73 -1.7 576,329 + IAI47_02780 0.73 +2.7 576,330 - IAI47_02780 0.73 -0.7 576,347 - IAI47_02780 0.78 +0.6 576,380 - IAI47_02780 0.87 -1.8 576,380 - IAI47_02780 0.87 +0.5 576,427 + -0.8 576,428 - +0.7 576,428 - -2.7 576,440 + -1.2 576,440 + +0.3 576,440 + +0.7 576,440 + -0.4 576,440 + +0.7 576,440 + -0.2 576,440 + -1.8 576,441 - +0.2 576,441 - +3.6 576,441 - +1.5 576,441 - +2.1 576,441 - -1.7 576,441 - -0.4 576,446 - +0.6 576,446 - -1.3 576,475 + IAI47_02785 0.16 +1.8 576,712 + +0.2 576,713 - -1.5 576,725 + -1.3 576,726 - -0.7 576,726 - -1.2 576,741 + +1.1 576,742 - -1.3 576,793 - -0.5 576,829 + +0.9 576,829 + -1.5 576,830 - -0.7 576,846 + +2.6 576,846 + +1.0 576,847 - +1.7 576,847 - -1.4 576,847 - -0.0 576,848 - -0.3 576,974 + IAI47_02790 0.15 -0.3 577,148 + IAI47_02790 0.38 -0.0 577,149 - IAI47_02790 0.38 +0.9 577,327 + IAI47_02790 0.61 +0.5 577,405 + IAI47_02790 0.71 -2.0 577,405 + IAI47_02790 0.71 +0.1 577,405 + IAI47_02790 0.71 -0.0 577,405 + IAI47_02790 0.71 -1.5 577,405 + IAI47_02790 0.71 +0.2 577,405 + IAI47_02790 0.71 -1.1 577,405 + IAI47_02790 0.71 +0.7 577,405 + IAI47_02790 0.71 +0.4 577,405 + IAI47_02790 0.71 -0.5 577,405 + IAI47_02790 0.71 +1.5 577,406 - IAI47_02790 0.71 -2.0 577,406 - IAI47_02790 0.71 +1.6 577,406 - IAI47_02790 0.71 +1.6 577,406 - IAI47_02790 0.71 -0.6 577,406 - IAI47_02790 0.71 -0.0 577,406 - IAI47_02790 0.71 +1.2 577,442 + IAI47_02790 0.76 -2.1 577,443 - IAI47_02790 0.76 -2.1 577,487 + IAI47_02790 0.81 -1.3 577,487 + IAI47_02790 0.81 -1.0 577,554 - -2.7 577,629 + -0.2 577,629 + +1.7 577,630 - +0.7 577,654 + -1.2 577,655 - -2.2 577,655 - -0.5 577,736 + IAI47_02795 0.14 -0.6 577,736 + IAI47_02795 0.14 +1.7 577,736 + IAI47_02795 0.14 +1.0 577,736 + IAI47_02795 0.14 +1.1 577,736 + IAI47_02795 0.14 -0.0 577,736 + IAI47_02795 0.14 -1.8 577,736 + IAI47_02795 0.14 +0.0 577,736 + IAI47_02795 0.14 +1.6 577,736 + IAI47_02795 0.14 -1.9 577,736 + IAI47_02795 0.14 +1.0 577,736 + IAI47_02795 0.14 -1.9 577,736 + IAI47_02795 0.14 -0.2 577,736 + IAI47_02795 0.14 -0.5 577,736 + IAI47_02795 0.14 +0.8 577,736 + IAI47_02795 0.14 -0.7 577,736 + IAI47_02795 0.14 +0.6 577,736 + IAI47_02795 0.14 +0.5 577,736 + IAI47_02795 0.14 +0.6 577,737 - IAI47_02795 0.15 -0.3 577,737 - IAI47_02795 0.15 +0.7 577,737 - IAI47_02795 0.15 +0.4 577,737 - IAI47_02795 0.15 +0.5 577,737 - IAI47_02795 0.15 +1.9 577,737 - IAI47_02795 0.15 +0.1 577,737 - IAI47_02795 0.15 +1.0 577,737 - IAI47_02795 0.15 -1.4 577,737 - IAI47_02795 0.15 +1.0 577,737 - IAI47_02795 0.15 +3.2 577,737 - IAI47_02795 0.15 -3.2 577,737 - IAI47_02795 0.15 -2.6 577,737 - IAI47_02795 0.15 +0.0 577,737 - IAI47_02795 0.15 +0.8 577,737 - IAI47_02795 0.15 +0.1 577,737 - IAI47_02795 0.15 +0.9 577,737 - IAI47_02795 0.15 -1.7 577,737 - IAI47_02795 0.15 +1.0 577,737 - IAI47_02795 0.15 +1.5 577,737 - IAI47_02795 0.15 +0.4 577,737 - IAI47_02795 0.15 -0.8 577,737 - IAI47_02795 0.15 +1.3 577,737 - IAI47_02795 0.15 -0.0 577,737 - IAI47_02795 0.15 -1.4 577,737 - IAI47_02795 0.15 -1.0 577,737 - IAI47_02795 0.15 -1.0 577,737 - IAI47_02795 0.15 +1.1 577,739 + IAI47_02795 0.15 +1.5 577,739 + IAI47_02795 0.15 +0.4 577,739 + IAI47_02795 0.15 -0.4 577,739 + IAI47_02795 0.15 +0.4 577,739 + IAI47_02795 0.15 +0.4 577,739 + IAI47_02795 0.15 +0.4 577,739 + IAI47_02795 0.15 +0.3 577,739 + IAI47_02795 0.15 +1.2 577,740 - IAI47_02795 0.15 +1.0 577,740 - IAI47_02795 0.15 -0.8 577,740 - IAI47_02795 0.15 +1.9 577,740 - IAI47_02795 0.15 -1.3 577,745 + IAI47_02795 0.16 +0.5 577,814 + IAI47_02795 0.27 +1.8 577,814 + IAI47_02795 0.27 +0.5 577,861 + IAI47_02795 0.34 +3.5 577,862 - IAI47_02795 0.34 +2.0 578,024 + IAI47_02795 0.59 +1.4 578,025 - IAI47_02795 0.60 +1.5 578,030 - IAI47_02795 0.60 -1.7 578,032 + IAI47_02795 0.61 +0.4 578,033 - IAI47_02795 0.61 -0.0 578,044 + IAI47_02795 0.62 -0.3 578,076 - IAI47_02795 0.67 -0.5 578,137 + IAI47_02795 0.77 +1.4 578,204 + IAI47_02795 0.87 +0.7 578,204 + IAI47_02795 0.87 +1.0 578,205 - IAI47_02795 0.88 -0.9 578,205 - IAI47_02795 0.88 -2.3 578,205 - IAI47_02795 0.88 +0.3 578,331 + -0.5 578,331 + -0.8 578,331 + +0.1 578,332 - +0.8 578,332 - +0.8 578,332 - -1.0 578,332 - +2.5 578,467 - IAI47_02800 0.12 +0.7 578,505 + IAI47_02800 0.16 +1.0 578,505 + IAI47_02800 0.16 +0.3 578,523 + IAI47_02800 0.17 -0.3 578,523 + IAI47_02800 0.17 +0.2 578,523 + IAI47_02800 0.17 -1.9 578,523 + IAI47_02800 0.17 +0.9 578,523 + IAI47_02800 0.17 +0.5 578,523 + IAI47_02800 0.17 +1.3 578,524 - IAI47_02800 0.17 +0.0 578,524 - IAI47_02800 0.17 -1.5 578,524 - IAI47_02800 0.17 -0.5 578,524 - IAI47_02800 0.17 -0.2 578,524 - IAI47_02800 0.17 -1.2 578,551 + IAI47_02800 0.20 -0.2 578,551 + IAI47_02800 0.20 -1.3 578,552 - IAI47_02800 0.20 -2.5 578,616 + IAI47_02800 0.25 +0.2
Or see this region's nucleotide sequence