Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_14755 and IAI47_14760 are separated by 55 nucleotides IAI47_14760 and IAI47_14765 are separated by 19 nucleotides IAI47_14765 and IAI47_14770 are separated by 90 nucleotides
IAI47_14755: IAI47_14755 - thiamine-phosphate kinase, at 3,113,044 to 3,114,021
_14755
IAI47_14760: IAI47_14760 - transcription antitermination factor NusB, at 3,114,077 to 3,114,496
_14760
IAI47_14765: IAI47_14765 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,114,516 to 3,114,986
_14765
IAI47_14770: IAI47_14770 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 3,115,077 to 3,116,177
_14770
Position (kb)
3114
3115 Strain fitness (log2 ratio)
-1
0
1 at 3114.022 kb on - strand at 3114.079 kb on - strand at 3115.036 kb on - strand at 3115.036 kb on - strand at 3115.036 kb on - strand at 3115.036 kb on - strand at 3115.041 kb on - strand at 3115.041 kb on - strand at 3115.078 kb on + strand at 3115.079 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 3,114,022 - +0.7 3,114,079 - +0.2 3,115,036 - -1.0 3,115,036 - -0.9 3,115,036 - -0.6 3,115,036 - +0.2 3,115,041 - +1.2 3,115,041 - -0.6 3,115,078 + +1.7 3,115,079 - +0.5
Or see this region's nucleotide sequence