Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_04135 and IAI47_04140 are separated by 149 nucleotides IAI47_04140 and IAI47_04145 are separated by 94 nucleotides IAI47_04145 and IAI47_04150 are separated by 298 nucleotides
IAI47_04135: IAI47_04135 - phosphopyruvate hydratase, at 869,057 to 870,352
_04135
IAI47_04140: IAI47_04140 - 7-carboxy-7-deazaguanine synthase QueE, at 870,502 to 871,173
_04140
IAI47_04145: IAI47_04145 - 6-carboxytetrahydropterin synthase QueD, at 871,268 to 871,627
_04145
IAI47_04150: IAI47_04150 - NADPH-dependent assimilatory sulfite reductase flavoprotein subunit, at 871,926 to 873,731
_04150
Position (kb) 871
872 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 870.310 kb on + strand at 870.339 kb on + strand at 870.340 kb on + strand at 870.340 kb on + strand at 870.351 kb on - strand at 870.351 kb on - strand at 870.351 kb on - strand at 870.389 kb on + strand at 870.390 kb on - strand at 870.390 kb on - strand at 870.467 kb on + strand at 870.467 kb on + strand at 870.467 kb on + strand at 870.467 kb on + strand at 870.467 kb on + strand at 870.467 kb on + strand at 870.468 kb on - strand at 870.468 kb on - strand at 870.468 kb on - strand at 870.468 kb on - strand at 870.468 kb on - strand at 870.550 kb on + strand at 870.550 kb on + strand at 870.551 kb on - strand at 870.551 kb on - strand at 870.551 kb on - strand at 870.553 kb on + strand at 870.553 kb on + strand at 870.553 kb on + strand at 870.553 kb on + strand at 870.553 kb on + strand at 870.554 kb on - strand at 870.554 kb on - strand at 870.554 kb on - strand at 870.554 kb on - strand at 870.554 kb on - strand at 870.554 kb on - strand at 870.554 kb on - strand at 870.554 kb on - strand at 870.554 kb on - strand at 870.554 kb on - strand at 870.584 kb on + strand, within IAI47_04140 at 870.584 kb on + strand, within IAI47_04140 at 870.584 kb on + strand, within IAI47_04140 at 870.585 kb on - strand, within IAI47_04140 at 870.606 kb on + strand, within IAI47_04140 at 870.606 kb on + strand, within IAI47_04140 at 870.606 kb on + strand, within IAI47_04140 at 870.606 kb on + strand, within IAI47_04140 at 870.606 kb on + strand, within IAI47_04140 at 870.606 kb on + strand, within IAI47_04140 at 870.606 kb on + strand, within IAI47_04140 at 870.607 kb on - strand, within IAI47_04140 at 870.607 kb on - strand, within IAI47_04140 at 870.607 kb on - strand, within IAI47_04140 at 870.607 kb on - strand, within IAI47_04140 at 870.669 kb on + strand, within IAI47_04140 at 870.669 kb on + strand, within IAI47_04140 at 870.774 kb on + strand, within IAI47_04140 at 870.797 kb on - strand, within IAI47_04140 at 870.935 kb on - strand, within IAI47_04140 at 870.935 kb on - strand, within IAI47_04140 at 870.935 kb on - strand, within IAI47_04140 at 871.034 kb on + strand, within IAI47_04140 at 871.034 kb on + strand, within IAI47_04140 at 871.034 kb on + strand, within IAI47_04140 at 871.034 kb on + strand, within IAI47_04140 at 871.034 kb on + strand, within IAI47_04140 at 871.035 kb on - strand, within IAI47_04140 at 871.035 kb on - strand, within IAI47_04140 at 871.044 kb on + strand, within IAI47_04140 at 871.044 kb on + strand, within IAI47_04140 at 871.044 kb on + strand, within IAI47_04140 at 871.044 kb on + strand, within IAI47_04140 at 871.045 kb on - strand, within IAI47_04140 at 871.045 kb on - strand, within IAI47_04140 at 871.064 kb on + strand, within IAI47_04140 at 871.064 kb on + strand, within IAI47_04140 at 871.064 kb on + strand, within IAI47_04140 at 871.064 kb on + strand, within IAI47_04140 at 871.126 kb on - strand at 871.126 kb on - strand at 871.152 kb on + strand at 871.153 kb on - strand at 871.153 kb on - strand at 871.156 kb on + strand at 871.156 kb on + strand at 871.156 kb on + strand at 871.156 kb on + strand at 871.157 kb on - strand at 871.164 kb on + strand at 871.176 kb on - strand at 871.181 kb on + strand at 871.181 kb on + strand at 871.182 kb on - strand at 871.182 kb on - strand at 871.193 kb on - strand at 871.236 kb on - strand at 871.236 kb on - strand at 871.269 kb on + strand at 871.322 kb on + strand, within IAI47_04145 at 871.322 kb on + strand, within IAI47_04145 at 871.323 kb on - strand, within IAI47_04145 at 871.402 kb on + strand, within IAI47_04145 at 871.402 kb on + strand, within IAI47_04145 at 871.403 kb on - strand, within IAI47_04145 at 871.403 kb on - strand, within IAI47_04145 at 871.403 kb on - strand, within IAI47_04145 at 871.403 kb on - strand, within IAI47_04145 at 871.403 kb on - strand, within IAI47_04145 at 871.403 kb on - strand, within IAI47_04145 at 871.413 kb on + strand, within IAI47_04145 at 871.414 kb on - strand, within IAI47_04145 at 871.414 kb on - strand, within IAI47_04145 at 871.414 kb on - strand, within IAI47_04145 at 871.414 kb on - strand, within IAI47_04145 at 871.414 kb on - strand, within IAI47_04145 at 871.416 kb on + strand, within IAI47_04145 at 871.417 kb on - strand, within IAI47_04145 at 871.417 kb on - strand, within IAI47_04145 at 871.417 kb on - strand, within IAI47_04145 at 871.432 kb on + strand, within IAI47_04145 at 871.433 kb on - strand, within IAI47_04145 at 871.433 kb on - strand, within IAI47_04145 at 871.434 kb on + strand, within IAI47_04145 at 871.434 kb on + strand, within IAI47_04145 at 871.434 kb on + strand, within IAI47_04145 at 871.434 kb on + strand, within IAI47_04145 at 871.434 kb on + strand, within IAI47_04145 at 871.434 kb on + strand, within IAI47_04145 at 871.435 kb on - strand, within IAI47_04145 at 871.435 kb on - strand, within IAI47_04145 at 871.435 kb on - strand, within IAI47_04145 at 871.445 kb on - strand, within IAI47_04145 at 871.487 kb on - strand, within IAI47_04145 at 871.508 kb on - strand, within IAI47_04145 at 871.508 kb on - strand, within IAI47_04145 at 871.577 kb on + strand, within IAI47_04145 at 871.578 kb on - strand, within IAI47_04145 at 871.578 kb on - strand, within IAI47_04145 at 871.578 kb on - strand, within IAI47_04145 at 871.609 kb on + strand at 871.609 kb on + strand at 871.610 kb on - strand at 871.610 kb on - strand at 871.621 kb on + strand at 871.621 kb on + strand at 871.621 kb on + strand at 871.621 kb on + strand at 871.622 kb on - strand at 871.627 kb on + strand at 871.627 kb on + strand at 871.627 kb on + strand at 871.627 kb on + strand at 871.627 kb on + strand at 871.627 kb on + strand at 871.627 kb on + strand at 871.627 kb on + strand at 871.627 kb on + strand at 871.628 kb on - strand at 871.628 kb on - strand at 871.628 kb on - strand at 871.628 kb on - strand at 871.628 kb on - strand at 871.628 kb on - strand at 871.663 kb on - strand at 871.670 kb on + strand at 871.722 kb on + strand at 871.722 kb on + strand at 871.723 kb on - strand at 871.751 kb on + strand at 871.752 kb on - strand at 871.752 kb on - strand at 871.752 kb on - strand at 871.769 kb on - strand at 871.784 kb on + strand at 871.784 kb on + strand at 871.784 kb on + strand at 871.785 kb on - strand at 871.790 kb on + strand at 871.806 kb on + strand at 871.806 kb on + strand at 871.819 kb on + strand at 871.820 kb on - strand at 871.826 kb on + strand at 871.827 kb on - strand at 871.827 kb on - strand at 871.827 kb on - strand at 871.839 kb on + strand at 871.839 kb on + strand at 871.839 kb on + strand at 871.840 kb on - strand at 871.840 kb on - strand at 871.840 kb on - strand at 871.840 kb on - strand at 871.844 kb on - strand at 871.844 kb on - strand at 871.844 kb on - strand at 871.850 kb on + strand at 871.851 kb on - strand at 871.851 kb on - strand at 871.853 kb on + strand at 871.854 kb on - strand at 871.854 kb on - strand at 871.854 kb on - strand at 871.854 kb on - strand at 871.854 kb on - strand at 871.854 kb on - strand at 871.854 kb on - strand at 871.854 kb on - strand at 871.855 kb on + strand at 871.855 kb on + strand at 871.856 kb on - strand at 871.856 kb on - strand at 871.856 kb on - strand at 871.870 kb on + strand at 871.870 kb on + strand at 871.870 kb on + strand at 871.870 kb on + strand at 871.870 kb on + strand at 871.907 kb on + strand at 871.907 kb on + strand at 871.949 kb on + strand at 871.949 kb on + strand at 871.949 kb on + strand at 871.949 kb on + strand at 871.949 kb on + strand at 871.949 kb on + strand at 871.949 kb on + strand at 871.949 kb on + strand at 871.949 kb on + strand at 871.949 kb on + strand at 871.949 kb on + strand at 871.949 kb on + strand at 871.950 kb on - strand at 871.950 kb on - strand at 871.950 kb on - strand at 871.950 kb on - strand at 871.950 kb on - strand at 871.950 kb on - strand at 871.950 kb on - strand at 871.950 kb on - strand at 871.950 kb on - strand at 871.950 kb on - strand at 871.985 kb on - strand at 872.004 kb on + strand at 872.005 kb on - strand at 872.038 kb on - strand at 872.054 kb on - strand at 872.054 kb on - strand at 872.069 kb on + strand at 872.088 kb on - strand at 872.088 kb on - strand at 872.117 kb on + strand, within IAI47_04150 at 872.117 kb on + strand, within IAI47_04150 at 872.118 kb on - strand, within IAI47_04150 at 872.203 kb on + strand, within IAI47_04150 at 872.203 kb on + strand, within IAI47_04150 at 872.204 kb on - strand, within IAI47_04150 at 872.226 kb on + strand, within IAI47_04150 at 872.226 kb on + strand, within IAI47_04150 at 872.226 kb on + strand, within IAI47_04150 at 872.227 kb on - strand, within IAI47_04150 at 872.227 kb on - strand, within IAI47_04150 at 872.227 kb on - strand, within IAI47_04150 at 872.227 kb on - strand, within IAI47_04150 at 872.227 kb on - strand, within IAI47_04150 at 872.321 kb on + strand, within IAI47_04150 at 872.321 kb on + strand, within IAI47_04150 at 872.321 kb on + strand, within IAI47_04150 at 872.322 kb on - strand, within IAI47_04150 at 872.322 kb on - strand, within IAI47_04150 at 872.493 kb on + strand, within IAI47_04150 at 872.493 kb on + strand, within IAI47_04150 at 872.494 kb on - strand, within IAI47_04150 at 872.494 kb on - strand, within IAI47_04150 at 872.622 kb on + strand, within IAI47_04150 at 872.623 kb on - strand, within IAI47_04150 at 872.623 kb on - strand, within IAI47_04150
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 870,310 + -1.7 870,339 + -0.6 870,340 + -0.6 870,340 + -0.5 870,351 - +0.5 870,351 - +1.0 870,351 - -0.8 870,389 + -1.0 870,390 - -1.3 870,390 - -1.2 870,467 + +0.9 870,467 + -0.8 870,467 + +0.4 870,467 + +0.5 870,467 + -0.2 870,467 + -0.4 870,468 - -1.5 870,468 - +0.8 870,468 - -0.6 870,468 - +0.2 870,468 - -1.0 870,550 + +0.1 870,550 + -1.5 870,551 - -0.6 870,551 - -0.8 870,551 - +0.6 870,553 + -0.8 870,553 + +0.8 870,553 + -0.8 870,553 + +1.7 870,553 + -0.5 870,554 - +0.5 870,554 - +0.3 870,554 - -0.5 870,554 - +0.1 870,554 - -0.1 870,554 - -0.9 870,554 - +0.1 870,554 - -1.7 870,554 - -0.5 870,554 - -0.2 870,584 + IAI47_04140 0.12 +1.0 870,584 + IAI47_04140 0.12 +0.5 870,584 + IAI47_04140 0.12 -1.0 870,585 - IAI47_04140 0.12 -0.8 870,606 + IAI47_04140 0.15 -0.4 870,606 + IAI47_04140 0.15 +0.3 870,606 + IAI47_04140 0.15 +0.1 870,606 + IAI47_04140 0.15 +0.3 870,606 + IAI47_04140 0.15 +0.4 870,606 + IAI47_04140 0.15 +2.1 870,606 + IAI47_04140 0.15 -0.2 870,607 - IAI47_04140 0.16 +0.9 870,607 - IAI47_04140 0.16 +0.2 870,607 - IAI47_04140 0.16 -0.4 870,607 - IAI47_04140 0.16 +0.6 870,669 + IAI47_04140 0.25 +0.1 870,669 + IAI47_04140 0.25 -2.0 870,774 + IAI47_04140 0.40 +0.2 870,797 - IAI47_04140 0.44 -2.8 870,935 - IAI47_04140 0.64 +0.1 870,935 - IAI47_04140 0.64 +1.1 870,935 - IAI47_04140 0.64 -1.2 871,034 + IAI47_04140 0.79 +1.3 871,034 + IAI47_04140 0.79 +0.1 871,034 + IAI47_04140 0.79 +0.3 871,034 + IAI47_04140 0.79 +0.3 871,034 + IAI47_04140 0.79 -1.4 871,035 - IAI47_04140 0.79 +0.4 871,035 - IAI47_04140 0.79 -0.5 871,044 + IAI47_04140 0.81 +0.2 871,044 + IAI47_04140 0.81 -0.1 871,044 + IAI47_04140 0.81 -1.5 871,044 + IAI47_04140 0.81 +0.1 871,045 - IAI47_04140 0.81 -1.5 871,045 - IAI47_04140 0.81 -0.3 871,064 + IAI47_04140 0.84 -2.1 871,064 + IAI47_04140 0.84 -0.2 871,064 + IAI47_04140 0.84 -0.7 871,064 + IAI47_04140 0.84 +0.4 871,126 - -1.9 871,126 - +0.6 871,152 + -0.4 871,153 - -0.5 871,153 - +0.6 871,156 + -0.7 871,156 + -0.7 871,156 + -0.3 871,156 + +0.4 871,157 - +1.2 871,164 + -0.1 871,176 - -0.5 871,181 + +0.5 871,181 + +0.6 871,182 - +0.6 871,182 - +0.1 871,193 - +0.6 871,236 - +1.3 871,236 - +0.6 871,269 + -0.4 871,322 + IAI47_04145 0.15 -0.1 871,322 + IAI47_04145 0.15 -1.7 871,323 - IAI47_04145 0.15 -1.1 871,402 + IAI47_04145 0.37 +0.2 871,402 + IAI47_04145 0.37 -1.5 871,403 - IAI47_04145 0.38 +0.4 871,403 - IAI47_04145 0.38 +0.5 871,403 - IAI47_04145 0.38 +1.8 871,403 - IAI47_04145 0.38 -0.3 871,403 - IAI47_04145 0.38 -1.8 871,403 - IAI47_04145 0.38 +0.7 871,413 + IAI47_04145 0.40 +1.3 871,414 - IAI47_04145 0.41 +0.1 871,414 - IAI47_04145 0.41 +0.8 871,414 - IAI47_04145 0.41 -0.3 871,414 - IAI47_04145 0.41 -1.2 871,414 - IAI47_04145 0.41 +0.3 871,416 + IAI47_04145 0.41 -1.5 871,417 - IAI47_04145 0.41 -0.3 871,417 - IAI47_04145 0.41 -0.3 871,417 - IAI47_04145 0.41 -1.0 871,432 + IAI47_04145 0.46 +0.3 871,433 - IAI47_04145 0.46 +0.7 871,433 - IAI47_04145 0.46 +0.2 871,434 + IAI47_04145 0.46 -0.5 871,434 + IAI47_04145 0.46 -0.4 871,434 + IAI47_04145 0.46 -0.8 871,434 + IAI47_04145 0.46 +0.1 871,434 + IAI47_04145 0.46 -0.1 871,434 + IAI47_04145 0.46 +0.1 871,435 - IAI47_04145 0.46 +0.5 871,435 - IAI47_04145 0.46 -1.2 871,435 - IAI47_04145 0.46 -0.6 871,445 - IAI47_04145 0.49 -1.2 871,487 - IAI47_04145 0.61 +0.1 871,508 - IAI47_04145 0.67 +0.5 871,508 - IAI47_04145 0.67 -0.3 871,577 + IAI47_04145 0.86 +1.1 871,578 - IAI47_04145 0.86 +1.8 871,578 - IAI47_04145 0.86 -1.0 871,578 - IAI47_04145 0.86 -3.1 871,609 + -0.5 871,609 + +1.8 871,610 - -0.9 871,610 - +0.8 871,621 + +1.1 871,621 + +0.9 871,621 + -0.8 871,621 + -0.7 871,622 - +1.3 871,627 + +0.6 871,627 + -0.1 871,627 + -0.0 871,627 + -0.0 871,627 + -0.4 871,627 + -0.0 871,627 + -2.3 871,627 + +0.3 871,627 + +0.5 871,628 - +0.2 871,628 - +1.4 871,628 - +0.2 871,628 - +0.4 871,628 - -0.0 871,628 - -0.3 871,663 - +1.1 871,670 + -1.6 871,722 + -1.7 871,722 + -2.0 871,723 - +0.1 871,751 + +2.1 871,752 - +0.4 871,752 - +0.2 871,752 - +0.3 871,769 - -1.0 871,784 + -0.2 871,784 + -0.6 871,784 + -1.0 871,785 - +0.9 871,790 + +0.9 871,806 + +0.7 871,806 + +0.5 871,819 + -1.0 871,820 - +0.4 871,826 + +0.4 871,827 - -0.9 871,827 - +0.8 871,827 - +1.1 871,839 + -0.0 871,839 + -2.1 871,839 + -1.2 871,840 - +2.1 871,840 - -1.3 871,840 - -0.5 871,840 - -0.4 871,844 - -0.1 871,844 - -1.6 871,844 - -0.3 871,850 + +0.1 871,851 - +1.1 871,851 - +0.2 871,853 + -0.5 871,854 - +1.1 871,854 - -1.0 871,854 - -0.5 871,854 - +1.2 871,854 - -1.8 871,854 - +0.1 871,854 - -0.8 871,854 - +0.3 871,855 + +0.8 871,855 + -0.9 871,856 - +0.5 871,856 - +1.5 871,856 - +0.3 871,870 + +0.4 871,870 + -0.2 871,870 + +0.3 871,870 + -1.4 871,870 + +0.7 871,907 + +1.3 871,907 + -0.5 871,949 + +0.2 871,949 + -0.8 871,949 + -1.3 871,949 + +0.1 871,949 + +0.5 871,949 + +1.2 871,949 + +0.5 871,949 + +0.1 871,949 + -0.1 871,949 + +0.3 871,949 + -0.5 871,949 + -0.4 871,950 - +1.1 871,950 - +0.5 871,950 - -1.5 871,950 - -2.0 871,950 - +1.7 871,950 - -1.6 871,950 - -0.5 871,950 - -2.8 871,950 - +0.6 871,950 - +0.8 871,985 - +0.3 872,004 + -1.2 872,005 - +0.4 872,038 - +1.8 872,054 - -1.2 872,054 - +1.6 872,069 + +0.3 872,088 - -1.2 872,088 - -0.1 872,117 + IAI47_04150 0.11 +0.5 872,117 + IAI47_04150 0.11 -0.7 872,118 - IAI47_04150 0.11 +0.8 872,203 + IAI47_04150 0.15 -1.3 872,203 + IAI47_04150 0.15 +1.1 872,204 - IAI47_04150 0.15 -0.1 872,226 + IAI47_04150 0.17 +0.5 872,226 + IAI47_04150 0.17 -1.1 872,226 + IAI47_04150 0.17 +1.9 872,227 - IAI47_04150 0.17 -0.3 872,227 - IAI47_04150 0.17 +0.7 872,227 - IAI47_04150 0.17 +0.8 872,227 - IAI47_04150 0.17 -1.5 872,227 - IAI47_04150 0.17 -1.7 872,321 + IAI47_04150 0.22 -0.1 872,321 + IAI47_04150 0.22 +0.9 872,321 + IAI47_04150 0.22 +1.1 872,322 - IAI47_04150 0.22 -0.1 872,322 - IAI47_04150 0.22 -0.7 872,493 + IAI47_04150 0.31 -0.2 872,493 + IAI47_04150 0.31 -0.2 872,494 - IAI47_04150 0.31 +0.1 872,494 - IAI47_04150 0.31 +0.4 872,622 + IAI47_04150 0.39 -1.4 872,623 - IAI47_04150 0.39 -0.0 872,623 - IAI47_04150 0.39 -0.5
Or see this region's nucleotide sequence