Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_10460 and IAI47_10465 are separated by 3 nucleotides IAI47_10465 and IAI47_10470 overlap by 8 nucleotides IAI47_10470 and IAI47_10475 overlap by 4 nucleotides IAI47_10475 and IAI47_10480 are separated by 205 nucleotides
IAI47_10460: IAI47_10460 - electron transport complex subunit RsxD, at 2,217,844 to 2,218,902
_10460
IAI47_10465: IAI47_10465 - electron transport complex subunit RsxG, at 2,218,906 to 2,219,538
_10465
IAI47_10470: IAI47_10470 - electron transport complex subunit E, at 2,219,531 to 2,220,235
_10470
IAI47_10475: IAI47_10475 - endonuclease III, at 2,220,232 to 2,220,864
_10475
IAI47_10480: IAI47_10480 - HPP family protein, at 2,221,070 to 2,221,789
_10480
Position (kb)
2219
2220
2221 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2218.861 kb on + strand at 2218.861 kb on + strand at 2218.861 kb on + strand at 2218.861 kb on + strand at 2218.861 kb on + strand at 2218.861 kb on + strand at 2218.861 kb on + strand at 2220.136 kb on + strand, within IAI47_10470 at 2220.140 kb on + strand, within IAI47_10470 at 2220.140 kb on + strand, within IAI47_10470 at 2220.243 kb on + strand at 2220.243 kb on + strand at 2220.244 kb on - strand at 2220.408 kb on + strand, within IAI47_10475 at 2220.408 kb on + strand, within IAI47_10475 at 2220.408 kb on + strand, within IAI47_10475 at 2220.409 kb on - strand, within IAI47_10475 at 2220.454 kb on + strand, within IAI47_10475 at 2220.454 kb on + strand, within IAI47_10475 at 2220.454 kb on + strand, within IAI47_10475 at 2220.454 kb on + strand at 2220.454 kb on + strand, within IAI47_10475 at 2220.454 kb on + strand, within IAI47_10475 at 2220.454 kb on + strand, within IAI47_10475 at 2220.455 kb on - strand, within IAI47_10475 at 2220.455 kb on - strand, within IAI47_10475 at 2220.455 kb on - strand, within IAI47_10475 at 2220.455 kb on - strand, within IAI47_10475 at 2220.501 kb on + strand, within IAI47_10475 at 2220.501 kb on + strand, within IAI47_10475 at 2220.501 kb on + strand, within IAI47_10475 at 2220.502 kb on - strand, within IAI47_10475 at 2220.502 kb on - strand, within IAI47_10475 at 2220.502 kb on - strand, within IAI47_10475 at 2220.502 kb on - strand, within IAI47_10475 at 2220.502 kb on - strand, within IAI47_10475 at 2220.502 kb on - strand, within IAI47_10475 at 2220.589 kb on - strand, within IAI47_10475 at 2220.613 kb on - strand, within IAI47_10475 at 2220.613 kb on - strand, within IAI47_10475 at 2220.645 kb on + strand, within IAI47_10475 at 2220.646 kb on - strand, within IAI47_10475 at 2220.646 kb on - strand, within IAI47_10475 at 2220.646 kb on - strand, within IAI47_10475 at 2220.646 kb on - strand, within IAI47_10475 at 2220.742 kb on + strand, within IAI47_10475 at 2220.742 kb on + strand, within IAI47_10475 at 2220.742 kb on + strand, within IAI47_10475 at 2220.743 kb on - strand, within IAI47_10475 at 2220.744 kb on + strand, within IAI47_10475 at 2220.744 kb on + strand, within IAI47_10475 at 2220.744 kb on + strand, within IAI47_10475 at 2220.845 kb on - strand at 2220.875 kb on + strand at 2220.919 kb on + strand at 2220.974 kb on + strand at 2220.974 kb on + strand at 2220.975 kb on - strand at 2220.975 kb on - strand at 2220.975 kb on - strand at 2220.998 kb on + strand at 2220.998 kb on + strand at 2220.998 kb on + strand at 2220.998 kb on + strand at 2220.998 kb on + strand at 2220.999 kb on - strand at 2220.999 kb on - strand at 2221.016 kb on - strand at 2221.039 kb on - strand at 2221.049 kb on + strand at 2221.050 kb on - strand at 2221.063 kb on + strand at 2221.068 kb on + strand at 2221.069 kb on - strand at 2221.147 kb on - strand, within IAI47_10480 at 2221.147 kb on - strand, within IAI47_10480 at 2221.150 kb on + strand, within IAI47_10480 at 2221.150 kb on + strand, within IAI47_10480 at 2221.198 kb on - strand, within IAI47_10480
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,218,861 + -1.7 2,218,861 + +0.2 2,218,861 + -1.6 2,218,861 + -1.6 2,218,861 + -1.2 2,218,861 + +4.0 2,218,861 + -0.1 2,220,136 + IAI47_10470 0.86 -0.9 2,220,140 + IAI47_10470 0.86 -1.1 2,220,140 + IAI47_10470 0.86 -2.1 2,220,243 + -0.3 2,220,243 + -1.3 2,220,244 - -0.9 2,220,408 + IAI47_10475 0.28 +0.5 2,220,408 + IAI47_10475 0.28 +3.1 2,220,408 + IAI47_10475 0.28 -0.4 2,220,409 - IAI47_10475 0.28 +1.2 2,220,454 + IAI47_10475 0.35 +1.4 2,220,454 + IAI47_10475 0.35 +0.4 2,220,454 + IAI47_10475 0.35 -1.4 2,220,454 + +0.4 2,220,454 + IAI47_10475 0.35 +0.4 2,220,454 + IAI47_10475 0.35 -2.0 2,220,454 + IAI47_10475 0.35 -0.1 2,220,455 - IAI47_10475 0.35 -0.4 2,220,455 - IAI47_10475 0.35 -1.8 2,220,455 - IAI47_10475 0.35 -2.7 2,220,455 - IAI47_10475 0.35 -0.8 2,220,501 + IAI47_10475 0.42 +1.0 2,220,501 + IAI47_10475 0.42 +0.2 2,220,501 + IAI47_10475 0.42 +1.4 2,220,502 - IAI47_10475 0.43 +0.8 2,220,502 - IAI47_10475 0.43 +1.1 2,220,502 - IAI47_10475 0.43 -0.9 2,220,502 - IAI47_10475 0.43 +0.8 2,220,502 - IAI47_10475 0.43 -0.8 2,220,502 - IAI47_10475 0.43 -0.4 2,220,589 - IAI47_10475 0.56 -2.7 2,220,613 - IAI47_10475 0.60 -2.1 2,220,613 - IAI47_10475 0.60 -1.5 2,220,645 + IAI47_10475 0.65 -2.5 2,220,646 - IAI47_10475 0.65 -2.5 2,220,646 - IAI47_10475 0.65 -1.8 2,220,646 - IAI47_10475 0.65 -0.8 2,220,646 - IAI47_10475 0.65 -0.4 2,220,742 + IAI47_10475 0.81 -0.6 2,220,742 + IAI47_10475 0.81 +0.1 2,220,742 + IAI47_10475 0.81 -1.7 2,220,743 - IAI47_10475 0.81 -0.2 2,220,744 + IAI47_10475 0.81 -1.7 2,220,744 + IAI47_10475 0.81 +1.0 2,220,744 + IAI47_10475 0.81 +1.8 2,220,845 - +1.2 2,220,875 + +0.2 2,220,919 + -0.6 2,220,974 + -2.3 2,220,974 + +0.1 2,220,975 - +1.0 2,220,975 - -1.3 2,220,975 - -0.8 2,220,998 + +0.4 2,220,998 + -0.5 2,220,998 + +3.3 2,220,998 + +0.3 2,220,998 + -0.6 2,220,999 - -0.3 2,220,999 - -2.0 2,221,016 - -0.9 2,221,039 - -0.1 2,221,049 + -2.1 2,221,050 - -1.1 2,221,063 + -0.9 2,221,068 + +1.8 2,221,069 - -1.7 2,221,147 - IAI47_10480 0.11 +0.9 2,221,147 - IAI47_10480 0.11 -0.2 2,221,150 + IAI47_10480 0.11 +1.3 2,221,150 + IAI47_10480 0.11 +1.8 2,221,198 - IAI47_10480 0.18 +0.6
Or see this region's nucleotide sequence