Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_04035 and IAI47_04040 are separated by 24 nucleotides IAI47_04040 and IAI47_04045 are separated by 34 nucleotides IAI47_04045 and IAI47_04050 are separated by 169 nucleotides
IAI47_04035: IAI47_04035 - 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM, at 843,333 to 844,433
_04035
IAI47_04040: IAI47_04040 - flap endonuclease Xni, at 844,458 to 845,213
_04040
IAI47_04045: IAI47_04045 - LOG family protein, at 845,248 to 846,612
_04045
IAI47_04050: IAI47_04050 - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 846,782 to 847,627
_04050
Position (kb) 845
846
847 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 844.248 kb on - strand, within IAI47_04035 at 844.248 kb on - strand, within IAI47_04035 at 844.248 kb on - strand, within IAI47_04035 at 844.248 kb on - strand, within IAI47_04035 at 844.248 kb on - strand, within IAI47_04035 at 844.248 kb on - strand, within IAI47_04035 at 844.269 kb on + strand, within IAI47_04035 at 844.269 kb on + strand, within IAI47_04035 at 844.270 kb on - strand, within IAI47_04035 at 844.270 kb on - strand, within IAI47_04035 at 844.356 kb on + strand at 844.356 kb on + strand at 844.356 kb on + strand at 844.357 kb on - strand at 844.357 kb on - strand at 844.357 kb on - strand at 844.357 kb on - strand at 844.394 kb on + strand at 844.394 kb on + strand at 844.394 kb on + strand at 844.395 kb on - strand at 844.395 kb on - strand at 844.395 kb on - strand at 844.395 kb on - strand at 844.468 kb on + strand at 844.532 kb on + strand at 844.532 kb on + strand at 844.532 kb on + strand at 844.532 kb on + strand at 844.532 kb on + strand at 844.533 kb on - strand at 844.544 kb on + strand, within IAI47_04040 at 844.544 kb on + strand, within IAI47_04040 at 844.544 kb on + strand, within IAI47_04040 at 844.544 kb on + strand, within IAI47_04040 at 844.544 kb on + strand, within IAI47_04040 at 844.544 kb on + strand, within IAI47_04040 at 844.545 kb on - strand, within IAI47_04040 at 844.545 kb on - strand, within IAI47_04040 at 844.545 kb on - strand, within IAI47_04040 at 844.545 kb on - strand, within IAI47_04040 at 844.545 kb on - strand, within IAI47_04040 at 844.545 kb on - strand, within IAI47_04040 at 844.545 kb on - strand, within IAI47_04040 at 844.545 kb on - strand, within IAI47_04040 at 844.545 kb on - strand, within IAI47_04040 at 844.603 kb on + strand, within IAI47_04040 at 844.603 kb on + strand, within IAI47_04040 at 844.604 kb on - strand, within IAI47_04040 at 844.604 kb on - strand, within IAI47_04040 at 844.604 kb on - strand, within IAI47_04040 at 844.604 kb on - strand, within IAI47_04040 at 844.632 kb on + strand, within IAI47_04040 at 844.632 kb on + strand, within IAI47_04040 at 844.652 kb on + strand, within IAI47_04040 at 844.701 kb on + strand, within IAI47_04040 at 844.701 kb on + strand, within IAI47_04040 at 844.702 kb on - strand, within IAI47_04040 at 844.702 kb on - strand, within IAI47_04040 at 844.702 kb on - strand, within IAI47_04040 at 844.711 kb on + strand, within IAI47_04040 at 844.712 kb on - strand, within IAI47_04040 at 844.712 kb on - strand, within IAI47_04040 at 844.754 kb on + strand, within IAI47_04040 at 844.754 kb on + strand, within IAI47_04040 at 844.786 kb on + strand, within IAI47_04040 at 844.787 kb on - strand, within IAI47_04040 at 844.787 kb on - strand, within IAI47_04040 at 844.787 kb on - strand, within IAI47_04040 at 844.826 kb on - strand, within IAI47_04040 at 844.877 kb on + strand, within IAI47_04040 at 844.878 kb on - strand, within IAI47_04040 at 844.896 kb on + strand, within IAI47_04040 at 844.935 kb on + strand, within IAI47_04040 at 844.935 kb on + strand, within IAI47_04040 at 844.935 kb on + strand, within IAI47_04040 at 844.935 kb on + strand, within IAI47_04040 at 844.936 kb on - strand, within IAI47_04040 at 844.936 kb on - strand, within IAI47_04040 at 845.017 kb on + strand, within IAI47_04040 at 845.070 kb on + strand, within IAI47_04040 at 845.071 kb on - strand, within IAI47_04040 at 845.107 kb on - strand, within IAI47_04040 at 845.154 kb on + strand at 845.154 kb on + strand at 845.154 kb on + strand at 845.154 kb on + strand at 845.155 kb on - strand at 845.155 kb on - strand at 845.195 kb on + strand at 845.195 kb on + strand at 845.196 kb on - strand at 845.196 kb on - strand at 845.196 kb on - strand at 845.220 kb on - strand at 845.249 kb on + strand at 845.249 kb on + strand at 845.249 kb on + strand at 845.250 kb on - strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.276 kb on + strand at 845.277 kb on + strand at 845.277 kb on - strand at 845.277 kb on - strand at 845.277 kb on - strand at 845.277 kb on - strand at 845.277 kb on - strand at 845.277 kb on - strand at 845.277 kb on - strand at 845.277 kb on - strand at 845.277 kb on - strand at 845.277 kb on - strand at 845.277 kb on - strand at 845.277 kb on - strand at 845.277 kb on - strand at 845.277 kb on - strand at 845.277 kb on - strand at 845.371 kb on - strand at 845.418 kb on + strand, within IAI47_04045 at 845.500 kb on - strand, within IAI47_04045 at 845.505 kb on + strand, within IAI47_04045 at 845.505 kb on + strand, within IAI47_04045 at 845.506 kb on - strand, within IAI47_04045 at 845.565 kb on + strand, within IAI47_04045 at 845.565 kb on + strand, within IAI47_04045 at 845.565 kb on + strand, within IAI47_04045 at 845.566 kb on - strand, within IAI47_04045 at 845.566 kb on - strand, within IAI47_04045 at 845.566 kb on - strand, within IAI47_04045 at 845.566 kb on - strand, within IAI47_04045 at 845.573 kb on + strand, within IAI47_04045 at 845.573 kb on + strand, within IAI47_04045 at 845.658 kb on + strand, within IAI47_04045 at 845.659 kb on - strand, within IAI47_04045 at 845.659 kb on - strand, within IAI47_04045 at 845.661 kb on + strand, within IAI47_04045 at 845.661 kb on + strand, within IAI47_04045 at 845.661 kb on + strand, within IAI47_04045 at 845.662 kb on - strand, within IAI47_04045 at 845.662 kb on - strand, within IAI47_04045 at 845.662 kb on - strand, within IAI47_04045 at 845.669 kb on + strand, within IAI47_04045 at 845.670 kb on - strand, within IAI47_04045 at 845.676 kb on + strand, within IAI47_04045 at 845.730 kb on - strand, within IAI47_04045 at 845.730 kb on - strand, within IAI47_04045 at 845.730 kb on - strand, within IAI47_04045 at 845.771 kb on - strand, within IAI47_04045 at 845.771 kb on - strand, within IAI47_04045 at 845.813 kb on + strand, within IAI47_04045 at 845.813 kb on + strand, within IAI47_04045 at 845.814 kb on - strand, within IAI47_04045 at 845.814 kb on - strand, within IAI47_04045 at 845.853 kb on + strand, within IAI47_04045 at 845.853 kb on + strand, within IAI47_04045 at 845.853 kb on + strand, within IAI47_04045 at 845.853 kb on + strand, within IAI47_04045 at 845.854 kb on - strand, within IAI47_04045 at 845.854 kb on - strand, within IAI47_04045 at 845.980 kb on - strand, within IAI47_04045 at 846.072 kb on + strand, within IAI47_04045 at 846.072 kb on + strand, within IAI47_04045 at 846.072 kb on + strand, within IAI47_04045 at 846.072 kb on + strand, within IAI47_04045 at 846.072 kb on + strand, within IAI47_04045 at 846.072 kb on + strand, within IAI47_04045 at 846.072 kb on + strand, within IAI47_04045 at 846.073 kb on - strand, within IAI47_04045 at 846.073 kb on - strand, within IAI47_04045 at 846.073 kb on - strand, within IAI47_04045 at 846.073 kb on - strand, within IAI47_04045 at 846.073 kb on - strand, within IAI47_04045 at 846.073 kb on - strand, within IAI47_04045 at 846.116 kb on + strand, within IAI47_04045 at 846.116 kb on + strand, within IAI47_04045 at 846.117 kb on - strand, within IAI47_04045 at 846.117 kb on - strand, within IAI47_04045 at 846.117 kb on - strand, within IAI47_04045 at 846.122 kb on + strand, within IAI47_04045 at 846.123 kb on - strand, within IAI47_04045 at 846.160 kb on + strand, within IAI47_04045 at 846.160 kb on + strand, within IAI47_04045 at 846.160 kb on + strand, within IAI47_04045 at 846.161 kb on - strand, within IAI47_04045 at 846.161 kb on - strand, within IAI47_04045 at 846.161 kb on - strand, within IAI47_04045 at 846.161 kb on - strand, within IAI47_04045 at 846.201 kb on + strand, within IAI47_04045 at 846.202 kb on - strand, within IAI47_04045 at 846.521 kb on + strand at 846.521 kb on + strand at 846.522 kb on - strand at 846.553 kb on - strand at 846.600 kb on + strand at 846.600 kb on + strand at 846.600 kb on + strand at 846.624 kb on + strand at 846.625 kb on - strand at 846.625 kb on - strand at 846.711 kb on + strand at 846.711 kb on + strand at 846.711 kb on + strand at 846.712 kb on - strand at 846.712 kb on - strand at 846.750 kb on + strand at 846.751 kb on - strand at 846.751 kb on - strand at 846.751 kb on - strand at 846.783 kb on + strand at 846.783 kb on + strand at 846.783 kb on + strand at 846.783 kb on + strand at 846.784 kb on - strand at 846.784 kb on - strand at 846.867 kb on + strand, within IAI47_04050 at 846.867 kb on + strand, within IAI47_04050 at 846.867 kb on + strand, within IAI47_04050 at 846.876 kb on + strand, within IAI47_04050 at 846.876 kb on + strand, within IAI47_04050 at 846.877 kb on - strand, within IAI47_04050 at 846.920 kb on - strand, within IAI47_04050 at 846.920 kb on - strand, within IAI47_04050 at 846.955 kb on + strand, within IAI47_04050 at 846.956 kb on - strand, within IAI47_04050 at 846.956 kb on - strand, within IAI47_04050 at 846.956 kb on - strand, within IAI47_04050 at 846.978 kb on + strand, within IAI47_04050 at 846.979 kb on - strand, within IAI47_04050 at 847.131 kb on + strand, within IAI47_04050 at 847.131 kb on + strand, within IAI47_04050 at 847.131 kb on + strand, within IAI47_04050 at 847.132 kb on - strand, within IAI47_04050 at 847.132 kb on - strand, within IAI47_04050 at 847.160 kb on - strand, within IAI47_04050 at 847.166 kb on - strand, within IAI47_04050 at 847.180 kb on + strand, within IAI47_04050 at 847.180 kb on + strand, within IAI47_04050 at 847.181 kb on - strand, within IAI47_04050 at 847.181 kb on - strand, within IAI47_04050 at 847.181 kb on - strand, within IAI47_04050 at 847.181 kb on - strand, within IAI47_04050 at 847.181 kb on - strand, within IAI47_04050 at 847.225 kb on + strand, within IAI47_04050 at 847.341 kb on + strand, within IAI47_04050 at 847.341 kb on + strand, within IAI47_04050 at 847.341 kb on + strand, within IAI47_04050 at 847.341 kb on + strand, within IAI47_04050 at 847.341 kb on + strand, within IAI47_04050 at 847.342 kb on - strand, within IAI47_04050 at 847.342 kb on - strand, within IAI47_04050 at 847.342 kb on - strand, within IAI47_04050 at 847.342 kb on - strand, within IAI47_04050 at 847.342 kb on - strand, within IAI47_04050 at 847.348 kb on + strand, within IAI47_04050 at 847.349 kb on - strand, within IAI47_04050 at 847.418 kb on + strand, within IAI47_04050 at 847.426 kb on + strand, within IAI47_04050 at 847.426 kb on + strand, within IAI47_04050 at 847.426 kb on + strand, within IAI47_04050 at 847.426 kb on + strand, within IAI47_04050 at 847.427 kb on - strand, within IAI47_04050 at 847.427 kb on - strand, within IAI47_04050 at 847.427 kb on - strand, within IAI47_04050 at 847.449 kb on + strand, within IAI47_04050 at 847.449 kb on + strand, within IAI47_04050 at 847.450 kb on - strand, within IAI47_04050 at 847.450 kb on - strand, within IAI47_04050 at 847.450 kb on - strand, within IAI47_04050 at 847.541 kb on + strand, within IAI47_04050 at 847.541 kb on + strand, within IAI47_04050 at 847.541 kb on + strand, within IAI47_04050 at 847.541 kb on + strand, within IAI47_04050 at 847.542 kb on - strand, within IAI47_04050 at 847.542 kb on - strand, within IAI47_04050 at 847.542 kb on - strand, within IAI47_04050 at 847.554 kb on + strand at 847.555 kb on - strand at 847.555 kb on - strand at 847.555 kb on - strand at 847.566 kb on + strand at 847.566 kb on + strand at 847.567 kb on - strand at 847.567 kb on - strand at 847.567 kb on - strand at 847.594 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 844,248 - IAI47_04035 0.83 +3.6 844,248 - IAI47_04035 0.83 -1.3 844,248 - IAI47_04035 0.83 +0.4 844,248 - IAI47_04035 0.83 -0.2 844,248 - IAI47_04035 0.83 -1.1 844,248 - IAI47_04035 0.83 -0.5 844,269 + IAI47_04035 0.85 -1.0 844,269 + IAI47_04035 0.85 -0.2 844,270 - IAI47_04035 0.85 +0.8 844,270 - IAI47_04035 0.85 -0.8 844,356 + +0.9 844,356 + +1.1 844,356 + -0.2 844,357 - +0.4 844,357 - +1.1 844,357 - -0.7 844,357 - -1.2 844,394 + -1.3 844,394 + -0.3 844,394 + +1.5 844,395 - -2.8 844,395 - +1.2 844,395 - -1.0 844,395 - -1.1 844,468 + +1.5 844,532 + -1.6 844,532 + -0.3 844,532 + +0.4 844,532 + +1.3 844,532 + -0.3 844,533 - +0.2 844,544 + IAI47_04040 0.11 -1.4 844,544 + IAI47_04040 0.11 -0.7 844,544 + IAI47_04040 0.11 -2.1 844,544 + IAI47_04040 0.11 -1.1 844,544 + IAI47_04040 0.11 +2.4 844,544 + IAI47_04040 0.11 -0.5 844,545 - IAI47_04040 0.12 +1.9 844,545 - IAI47_04040 0.12 -0.0 844,545 - IAI47_04040 0.12 +0.9 844,545 - IAI47_04040 0.12 -0.9 844,545 - IAI47_04040 0.12 -1.1 844,545 - IAI47_04040 0.12 +0.2 844,545 - IAI47_04040 0.12 +1.2 844,545 - IAI47_04040 0.12 +0.5 844,545 - IAI47_04040 0.12 -1.0 844,603 + IAI47_04040 0.19 -0.3 844,603 + IAI47_04040 0.19 -1.7 844,604 - IAI47_04040 0.19 -0.7 844,604 - IAI47_04040 0.19 +0.7 844,604 - IAI47_04040 0.19 -0.8 844,604 - IAI47_04040 0.19 -0.3 844,632 + IAI47_04040 0.23 +0.4 844,632 + IAI47_04040 0.23 -1.5 844,652 + IAI47_04040 0.26 -0.1 844,701 + IAI47_04040 0.32 +0.9 844,701 + IAI47_04040 0.32 -2.9 844,702 - IAI47_04040 0.32 -0.9 844,702 - IAI47_04040 0.32 -0.7 844,702 - IAI47_04040 0.32 -1.8 844,711 + IAI47_04040 0.33 +0.9 844,712 - IAI47_04040 0.34 -1.5 844,712 - IAI47_04040 0.34 -2.6 844,754 + IAI47_04040 0.39 +0.2 844,754 + IAI47_04040 0.39 +0.2 844,786 + IAI47_04040 0.43 -2.1 844,787 - IAI47_04040 0.44 -1.7 844,787 - IAI47_04040 0.44 +0.4 844,787 - IAI47_04040 0.44 +2.8 844,826 - IAI47_04040 0.49 +0.8 844,877 + IAI47_04040 0.55 +1.5 844,878 - IAI47_04040 0.56 +0.8 844,896 + IAI47_04040 0.58 +1.5 844,935 + IAI47_04040 0.63 +0.3 844,935 + IAI47_04040 0.63 +1.4 844,935 + IAI47_04040 0.63 -1.3 844,935 + IAI47_04040 0.63 +2.1 844,936 - IAI47_04040 0.63 +1.9 844,936 - IAI47_04040 0.63 +0.7 845,017 + IAI47_04040 0.74 +1.2 845,070 + IAI47_04040 0.81 -1.1 845,071 - IAI47_04040 0.81 +0.4 845,107 - IAI47_04040 0.86 -1.1 845,154 + -1.5 845,154 + +0.8 845,154 + +2.2 845,154 + -2.2 845,155 - -0.3 845,155 - +0.4 845,195 + -1.3 845,195 + -0.1 845,196 - +0.9 845,196 - +0.3 845,196 - +0.1 845,220 - -1.3 845,249 + +0.6 845,249 + -0.3 845,249 + +1.5 845,250 - +1.2 845,276 + -0.2 845,276 + +1.7 845,276 + +2.4 845,276 + -0.1 845,276 + -0.7 845,276 + -0.1 845,276 + -1.0 845,276 + +1.5 845,276 + +2.1 845,276 + -1.2 845,276 + -0.6 845,276 + -0.9 845,276 + +2.0 845,276 + +1.9 845,276 + -1.5 845,276 + +0.3 845,276 + -1.7 845,276 + +0.1 845,276 + +0.9 845,277 + -0.5 845,277 - -2.0 845,277 - +0.2 845,277 - +0.4 845,277 - +0.4 845,277 - +1.1 845,277 - +0.6 845,277 - -1.5 845,277 - +1.2 845,277 - +0.7 845,277 - -0.2 845,277 - +0.3 845,277 - -0.9 845,277 - -2.0 845,277 - -2.9 845,277 - -0.1 845,371 - +0.1 845,418 + IAI47_04045 0.12 -1.8 845,500 - IAI47_04045 0.18 +2.1 845,505 + IAI47_04045 0.19 -1.7 845,505 + IAI47_04045 0.19 -1.2 845,506 - IAI47_04045 0.19 +1.9 845,565 + IAI47_04045 0.23 +1.2 845,565 + IAI47_04045 0.23 +0.2 845,565 + IAI47_04045 0.23 -1.5 845,566 - IAI47_04045 0.23 +1.5 845,566 - IAI47_04045 0.23 +1.1 845,566 - IAI47_04045 0.23 -2.8 845,566 - IAI47_04045 0.23 +1.1 845,573 + IAI47_04045 0.24 -0.0 845,573 + IAI47_04045 0.24 -1.5 845,658 + IAI47_04045 0.30 +0.3 845,659 - IAI47_04045 0.30 -0.7 845,659 - IAI47_04045 0.30 +0.1 845,661 + IAI47_04045 0.30 -2.0 845,661 + IAI47_04045 0.30 -2.7 845,661 + IAI47_04045 0.30 -0.3 845,662 - IAI47_04045 0.30 -0.1 845,662 - IAI47_04045 0.30 -0.2 845,662 - IAI47_04045 0.30 +2.4 845,669 + IAI47_04045 0.31 -0.6 845,670 - IAI47_04045 0.31 -0.3 845,676 + IAI47_04045 0.31 -0.9 845,730 - IAI47_04045 0.35 +0.5 845,730 - IAI47_04045 0.35 -2.9 845,730 - IAI47_04045 0.35 +2.2 845,771 - IAI47_04045 0.38 +1.4 845,771 - IAI47_04045 0.38 +0.3 845,813 + IAI47_04045 0.41 -0.1 845,813 + IAI47_04045 0.41 +2.4 845,814 - IAI47_04045 0.41 +0.2 845,814 - IAI47_04045 0.41 -1.1 845,853 + IAI47_04045 0.44 -0.5 845,853 + IAI47_04045 0.44 +1.4 845,853 + IAI47_04045 0.44 +0.7 845,853 + IAI47_04045 0.44 +2.2 845,854 - IAI47_04045 0.44 -0.3 845,854 - IAI47_04045 0.44 +2.2 845,980 - IAI47_04045 0.54 +0.2 846,072 + IAI47_04045 0.60 +1.3 846,072 + IAI47_04045 0.60 -0.7 846,072 + IAI47_04045 0.60 +0.9 846,072 + IAI47_04045 0.60 +1.0 846,072 + IAI47_04045 0.60 -0.1 846,072 + IAI47_04045 0.60 -2.1 846,072 + IAI47_04045 0.60 +1.9 846,073 - IAI47_04045 0.60 +1.6 846,073 - IAI47_04045 0.60 -0.3 846,073 - IAI47_04045 0.60 +0.7 846,073 - IAI47_04045 0.60 +1.5 846,073 - IAI47_04045 0.60 +0.6 846,073 - IAI47_04045 0.60 -2.3 846,116 + IAI47_04045 0.64 +1.1 846,116 + IAI47_04045 0.64 +0.5 846,117 - IAI47_04045 0.64 +0.5 846,117 - 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IAI47_04050 0.67 -0.2 847,418 + IAI47_04050 0.75 +1.2 847,426 + IAI47_04050 0.76 -0.3 847,426 + IAI47_04050 0.76 +0.6 847,426 + IAI47_04050 0.76 +0.3 847,426 + IAI47_04050 0.76 +1.4 847,427 - IAI47_04050 0.76 -1.8 847,427 - IAI47_04050 0.76 +1.5 847,427 - IAI47_04050 0.76 +1.7 847,449 + IAI47_04050 0.79 +1.3 847,449 + IAI47_04050 0.79 +1.0 847,450 - IAI47_04050 0.79 -1.2 847,450 - IAI47_04050 0.79 -1.1 847,450 - IAI47_04050 0.79 -1.1 847,541 + IAI47_04050 0.90 +1.4 847,541 + IAI47_04050 0.90 -2.1 847,541 + IAI47_04050 0.90 +1.2 847,541 + IAI47_04050 0.90 +1.4 847,542 - IAI47_04050 0.90 +0.4 847,542 - IAI47_04050 0.90 +3.0 847,542 - IAI47_04050 0.90 +1.2 847,554 + -1.7 847,555 - +2.3 847,555 - +3.2 847,555 - -1.1 847,566 + +0.4 847,566 + +0.7 847,567 - +0.2 847,567 - +0.7 847,567 - -1.0 847,594 + +1.2
Or see this region's nucleotide sequence