Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_03820 and IAI47_03825 are separated by 21 nucleotides IAI47_03825 and IAI47_03830 are separated by 3 nucleotides IAI47_03830 and IAI47_03835 are separated by 231 nucleotides
IAI47_03820: IAI47_03820 - site-specific tyrosine recombinase XerD, at 796,083 to 796,976
_03820
IAI47_03825: IAI47_03825 - bifunctional protein-disulfide isomerase/oxidoreductase DsbC, at 796,998 to 797,711
_03825
IAI47_03830: IAI47_03830 - single-stranded-DNA-specific exonuclease RecJ, at 797,715 to 799,451
_03830
IAI47_03835: IAI47_03835 - peptide chain release factor 2, at 799,683 to 800,781
_03835
Position (kb) 797
798
799
800 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 796.768 kb on - strand, within IAI47_03820 at 796.813 kb on + strand, within IAI47_03820 at 796.813 kb on + strand, within IAI47_03820 at 796.814 kb on - strand, within IAI47_03820 at 796.814 kb on - strand, within IAI47_03820 at 796.889 kb on + strand at 796.889 kb on + strand at 796.890 kb on - strand at 796.890 kb on - strand at 796.987 kb on + strand at 796.987 kb on + strand at 796.988 kb on - strand at 796.988 kb on - strand at 796.988 kb on - strand at 796.994 kb on - strand at 797.088 kb on - strand, within IAI47_03825 at 797.172 kb on + strand, within IAI47_03825 at 797.172 kb on + strand, within IAI47_03825 at 797.172 kb on + strand, within IAI47_03825 at 797.172 kb on + strand, within IAI47_03825 at 797.173 kb on - strand, within IAI47_03825 at 797.173 kb on - strand, within IAI47_03825 at 797.173 kb on - strand, within IAI47_03825 at 797.173 kb on - strand, within IAI47_03825 at 797.173 kb on - strand, within IAI47_03825 at 797.173 kb on - strand, within IAI47_03825 at 797.173 kb on - strand, within IAI47_03825 at 797.173 kb on - strand, within IAI47_03825 at 797.173 kb on - strand, within IAI47_03825 at 797.177 kb on + strand, within IAI47_03825 at 797.195 kb on + strand, within IAI47_03825 at 797.195 kb on + strand, within IAI47_03825 at 797.195 kb on + strand, within IAI47_03825 at 797.195 kb on + strand, within IAI47_03825 at 797.195 kb on + strand, within IAI47_03825 at 797.195 kb on + strand, within IAI47_03825 at 797.196 kb on - strand, within IAI47_03825 at 797.196 kb on - strand, within IAI47_03825 at 797.196 kb on - strand, within IAI47_03825 at 797.196 kb on - strand, within IAI47_03825 at 797.196 kb on - strand, within IAI47_03825 at 797.196 kb on - strand, within IAI47_03825 at 797.196 kb on - strand, within IAI47_03825 at 797.196 kb on - strand, within IAI47_03825 at 797.196 kb on - strand, within IAI47_03825 at 797.214 kb on + strand, within IAI47_03825 at 797.214 kb on + strand, within IAI47_03825 at 797.214 kb on + strand, within IAI47_03825 at 797.214 kb on + strand, within IAI47_03825 at 797.215 kb on - strand, within IAI47_03825 at 797.215 kb on - strand, within IAI47_03825 at 797.301 kb on + strand, within IAI47_03825 at 797.301 kb on + strand, within IAI47_03825 at 797.301 kb on + strand, within IAI47_03825 at 797.302 kb on - strand, within IAI47_03825 at 797.358 kb on + strand, within IAI47_03825 at 797.358 kb on + strand, within IAI47_03825 at 797.358 kb on + strand, within IAI47_03825 at 797.391 kb on + strand, within IAI47_03825 at 797.391 kb on + strand, within IAI47_03825 at 797.391 kb on + strand, within IAI47_03825 at 797.391 kb on + strand, within IAI47_03825 at 797.391 kb on + strand, within IAI47_03825 at 797.392 kb on - strand, within IAI47_03825 at 797.408 kb on + strand, within IAI47_03825 at 797.408 kb on + strand, within IAI47_03825 at 797.409 kb on - strand, within IAI47_03825 at 797.418 kb on + strand, within IAI47_03825 at 797.498 kb on + strand, within IAI47_03825 at 797.498 kb on + strand, within IAI47_03825 at 797.499 kb on - strand, within IAI47_03825 at 797.499 kb on - strand, within IAI47_03825 at 797.499 kb on - strand, within IAI47_03825 at 797.567 kb on + strand, within IAI47_03825 at 797.580 kb on + strand, within IAI47_03825 at 797.581 kb on - strand, within IAI47_03825 at 797.581 kb on - strand, within IAI47_03825 at 797.581 kb on - strand, within IAI47_03825 at 797.655 kb on + strand at 797.655 kb on - strand at 797.656 kb on - strand at 797.719 kb on + strand at 797.719 kb on + strand at 797.720 kb on - strand at 797.805 kb on + strand at 797.805 kb on + strand at 797.806 kb on - strand at 797.806 kb on - strand at 797.868 kb on + strand at 797.868 kb on + strand at 797.869 kb on - strand at 797.939 kb on + strand, within IAI47_03830 at 797.939 kb on + strand, within IAI47_03830 at 797.939 kb on + strand, within IAI47_03830 at 797.940 kb on - strand, within IAI47_03830 at 797.940 kb on - strand, within IAI47_03830 at 797.940 kb on - strand, within IAI47_03830 at 797.978 kb on + strand, within IAI47_03830 at 798.030 kb on + strand, within IAI47_03830 at 798.030 kb on + strand, within IAI47_03830 at 798.030 kb on + strand, within IAI47_03830 at 798.030 kb on + strand, within IAI47_03830 at 798.030 kb on + strand, within IAI47_03830 at 798.030 kb on + strand, within IAI47_03830 at 798.306 kb on + strand, within IAI47_03830 at 798.306 kb on + strand, within IAI47_03830 at 798.306 kb on + strand, within IAI47_03830 at 798.307 kb on - strand, within IAI47_03830 at 798.307 kb on - strand, within IAI47_03830 at 798.307 kb on - strand, within IAI47_03830 at 798.527 kb on + strand, within IAI47_03830 at 798.527 kb on + strand, within IAI47_03830 at 798.611 kb on + strand, within IAI47_03830 at 798.611 kb on + strand, within IAI47_03830 at 798.611 kb on + strand, within IAI47_03830 at 798.612 kb on - strand, within IAI47_03830 at 798.612 kb on - strand, within IAI47_03830 at 798.612 kb on - strand, within IAI47_03830 at 798.612 kb on - strand, within IAI47_03830 at 798.634 kb on + strand, within IAI47_03830 at 798.635 kb on - strand, within IAI47_03830 at 798.795 kb on + strand, within IAI47_03830 at 798.795 kb on + strand, within IAI47_03830 at 798.795 kb on + strand, within IAI47_03830 at 798.795 kb on + strand, within IAI47_03830 at 798.795 kb on + strand, within IAI47_03830 at 798.795 kb on + strand, within IAI47_03830 at 798.795 kb on + strand, within IAI47_03830 at 798.796 kb on - strand, within IAI47_03830 at 798.796 kb on - strand, within IAI47_03830 at 798.796 kb on - strand, within IAI47_03830 at 798.796 kb on - strand, within IAI47_03830 at 798.796 kb on - strand, within IAI47_03830 at 798.796 kb on - strand, within IAI47_03830 at 798.796 kb on - strand, within IAI47_03830 at 798.796 kb on - strand, within IAI47_03830 at 798.846 kb on - strand, within IAI47_03830 at 798.977 kb on + strand, within IAI47_03830 at 798.977 kb on + strand, within IAI47_03830 at 798.977 kb on + strand, within IAI47_03830 at 798.978 kb on - strand, within IAI47_03830 at 798.978 kb on - strand, within IAI47_03830 at 798.986 kb on - strand, within IAI47_03830 at 798.986 kb on - strand, within IAI47_03830 at 798.986 kb on - strand, within IAI47_03830 at 799.019 kb on - strand, within IAI47_03830 at 799.019 kb on - strand, within IAI47_03830 at 799.019 kb on - strand, within IAI47_03830 at 799.227 kb on - strand, within IAI47_03830 at 799.265 kb on + strand, within IAI47_03830 at 799.265 kb on + strand, within IAI47_03830 at 799.265 kb on + strand, within IAI47_03830 at 799.265 kb on + strand, within IAI47_03830 at 799.265 kb on + strand, within IAI47_03830 at 799.265 kb on + strand, within IAI47_03830 at 799.265 kb on + strand, within IAI47_03830 at 799.266 kb on - strand, within IAI47_03830 at 799.266 kb on - strand, within IAI47_03830 at 799.266 kb on - strand, within IAI47_03830 at 799.266 kb on - strand, within IAI47_03830 at 799.266 kb on - strand, within IAI47_03830 at 799.266 kb on - strand, within IAI47_03830 at 799.319 kb on + strand at 799.319 kb on + strand at 799.319 kb on + strand at 799.320 kb on - strand at 799.320 kb on - strand at 799.320 kb on - strand at 799.320 kb on - strand at 799.320 kb on - strand at 799.320 kb on - strand at 799.328 kb on + strand at 799.328 kb on + strand at 799.329 kb on - strand at 799.329 kb on - strand at 799.374 kb on + strand at 799.374 kb on + strand at 799.374 kb on + strand at 799.374 kb on + strand at 799.375 kb on - strand at 799.375 kb on - strand at 799.376 kb on + strand at 799.376 kb on + strand at 799.376 kb on + strand at 799.376 kb on + strand at 799.376 kb on + strand at 799.376 kb on + strand at 799.377 kb on - strand at 799.377 kb on - strand at 799.377 kb on - strand at 799.377 kb on - strand at 799.377 kb on - strand at 799.377 kb on - strand at 799.377 kb on - strand at 799.385 kb on + strand at 799.386 kb on - strand at 799.395 kb on + strand at 799.396 kb on - strand at 799.396 kb on - strand at 799.433 kb on + strand at 799.433 kb on + strand at 799.433 kb on + strand at 799.434 kb on - strand at 799.434 kb on - strand at 799.434 kb on - strand at 799.434 kb on - strand at 799.434 kb on - strand at 799.434 kb on - strand at 799.434 kb on - strand at 799.434 kb on - strand at 799.454 kb on + strand at 799.517 kb on - strand at 799.605 kb on + strand at 799.606 kb on - strand at 799.606 kb on - strand at 799.664 kb on + strand at 799.664 kb on + strand at 799.664 kb on + strand at 799.664 kb on + strand at 799.664 kb on + strand at 799.664 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 796,768 - IAI47_03820 0.77 -0.3 796,813 + IAI47_03820 0.82 +0.8 796,813 + IAI47_03820 0.82 -0.4 796,814 - IAI47_03820 0.82 -1.6 796,814 - IAI47_03820 0.82 -0.2 796,889 + -0.7 796,889 + +1.0 796,890 - -0.7 796,890 - -1.2 796,987 + -1.6 796,987 + -2.4 796,988 - -1.0 796,988 - -0.9 796,988 - +0.6 796,994 - -0.8 797,088 - IAI47_03825 0.13 +3.6 797,172 + IAI47_03825 0.24 +0.8 797,172 + IAI47_03825 0.24 +0.2 797,172 + IAI47_03825 0.24 -0.4 797,172 + IAI47_03825 0.24 -0.7 797,173 - IAI47_03825 0.25 +2.8 797,173 - IAI47_03825 0.25 +0.9 797,173 - IAI47_03825 0.25 -2.4 797,173 - IAI47_03825 0.25 -1.5 797,173 - IAI47_03825 0.25 -1.1 797,173 - IAI47_03825 0.25 +0.6 797,173 - IAI47_03825 0.25 -0.9 797,173 - IAI47_03825 0.25 +1.5 797,173 - IAI47_03825 0.25 -0.3 797,177 + IAI47_03825 0.25 +0.5 797,195 + IAI47_03825 0.28 -0.0 797,195 + IAI47_03825 0.28 +0.8 797,195 + IAI47_03825 0.28 +1.5 797,195 + IAI47_03825 0.28 +3.0 797,195 + IAI47_03825 0.28 -1.6 797,195 + IAI47_03825 0.28 +0.6 797,196 - IAI47_03825 0.28 -0.0 797,196 - IAI47_03825 0.28 -1.5 797,196 - IAI47_03825 0.28 +0.6 797,196 - IAI47_03825 0.28 -1.2 797,196 - IAI47_03825 0.28 +1.3 797,196 - IAI47_03825 0.28 +2.1 797,196 - IAI47_03825 0.28 -0.5 797,196 - IAI47_03825 0.28 -1.6 797,196 - IAI47_03825 0.28 +0.6 797,214 + IAI47_03825 0.30 +1.4 797,214 + IAI47_03825 0.30 -1.2 797,214 + IAI47_03825 0.30 -0.8 797,214 + IAI47_03825 0.30 -0.2 797,215 - IAI47_03825 0.30 +2.0 797,215 - IAI47_03825 0.30 +0.5 797,301 + IAI47_03825 0.42 +1.2 797,301 + IAI47_03825 0.42 -0.9 797,301 + IAI47_03825 0.42 +0.5 797,302 - IAI47_03825 0.43 -0.4 797,358 + IAI47_03825 0.50 +0.6 797,358 + IAI47_03825 0.50 -0.0 797,358 + IAI47_03825 0.50 +1.7 797,391 + IAI47_03825 0.55 +1.2 797,391 + IAI47_03825 0.55 +4.3 797,391 + IAI47_03825 0.55 -0.9 797,391 + IAI47_03825 0.55 -1.6 797,391 + IAI47_03825 0.55 +1.0 797,392 - IAI47_03825 0.55 -1.6 797,408 + IAI47_03825 0.57 -1.4 797,408 + IAI47_03825 0.57 +2.6 797,409 - IAI47_03825 0.58 +0.6 797,418 + IAI47_03825 0.59 -1.2 797,498 + IAI47_03825 0.70 -0.4 797,498 + IAI47_03825 0.70 -1.5 797,499 - IAI47_03825 0.70 -0.4 797,499 - IAI47_03825 0.70 -2.2 797,499 - IAI47_03825 0.70 +0.2 797,567 + IAI47_03825 0.80 +0.8 797,580 + IAI47_03825 0.82 -0.1 797,581 - IAI47_03825 0.82 +1.9 797,581 - IAI47_03825 0.82 +1.0 797,581 - IAI47_03825 0.82 -2.0 797,655 + +2.0 797,655 - -1.7 797,656 - +0.2 797,719 + -0.7 797,719 + +2.0 797,720 - -0.6 797,805 + -1.6 797,805 + -0.8 797,806 - +2.9 797,806 - -2.3 797,868 + -0.9 797,868 + -1.4 797,869 - -2.0 797,939 + IAI47_03830 0.13 +0.8 797,939 + IAI47_03830 0.13 -1.6 797,939 + IAI47_03830 0.13 -0.7 797,940 - IAI47_03830 0.13 +0.6 797,940 - IAI47_03830 0.13 -0.6 797,940 - IAI47_03830 0.13 -0.2 797,978 + IAI47_03830 0.15 -1.6 798,030 + IAI47_03830 0.18 +0.0 798,030 + IAI47_03830 0.18 +0.2 798,030 + IAI47_03830 0.18 -1.2 798,030 + IAI47_03830 0.18 -0.8 798,030 + IAI47_03830 0.18 +0.3 798,030 + IAI47_03830 0.18 +1.4 798,306 + IAI47_03830 0.34 -0.5 798,306 + IAI47_03830 0.34 -0.4 798,306 + IAI47_03830 0.34 -0.2 798,307 - IAI47_03830 0.34 +0.4 798,307 - IAI47_03830 0.34 -0.5 798,307 - IAI47_03830 0.34 +2.3 798,527 + IAI47_03830 0.47 +1.4 798,527 + IAI47_03830 0.47 -0.9 798,611 + IAI47_03830 0.52 -1.5 798,611 + IAI47_03830 0.52 -1.2 798,611 + IAI47_03830 0.52 -0.8 798,612 - IAI47_03830 0.52 -1.7 798,612 - IAI47_03830 0.52 +0.6 798,612 - IAI47_03830 0.52 -1.1 798,612 - IAI47_03830 0.52 -0.9 798,634 + IAI47_03830 0.53 -0.4 798,635 - IAI47_03830 0.53 -1.6 798,795 + IAI47_03830 0.62 -0.5 798,795 + IAI47_03830 0.62 +0.2 798,795 + IAI47_03830 0.62 +0.4 798,795 + IAI47_03830 0.62 +1.6 798,795 + IAI47_03830 0.62 -0.5 798,795 + IAI47_03830 0.62 +0.3 798,795 + IAI47_03830 0.62 -0.3 798,796 - IAI47_03830 0.62 -0.9 798,796 - IAI47_03830 0.62 -0.2 798,796 - IAI47_03830 0.62 -2.1 798,796 - IAI47_03830 0.62 +2.6 798,796 - IAI47_03830 0.62 -0.7 798,796 - IAI47_03830 0.62 -1.9 798,796 - IAI47_03830 0.62 -2.8 798,796 - IAI47_03830 0.62 -0.2 798,846 - IAI47_03830 0.65 -1.2 798,977 + IAI47_03830 0.73 -0.7 798,977 + IAI47_03830 0.73 +2.9 798,977 + IAI47_03830 0.73 -3.1 798,978 - IAI47_03830 0.73 -2.4 798,978 - IAI47_03830 0.73 -0.2 798,986 - IAI47_03830 0.73 +0.7 798,986 - IAI47_03830 0.73 +0.2 798,986 - IAI47_03830 0.73 -1.2 799,019 - IAI47_03830 0.75 -0.5 799,019 - IAI47_03830 0.75 -0.2 799,019 - IAI47_03830 0.75 +1.7 799,227 - IAI47_03830 0.87 -2.7 799,265 + IAI47_03830 0.89 -1.8 799,265 + IAI47_03830 0.89 -0.3 799,265 + IAI47_03830 0.89 -1.4 799,265 + IAI47_03830 0.89 +0.6 799,265 + IAI47_03830 0.89 +1.7 799,265 + IAI47_03830 0.89 +0.6 799,265 + IAI47_03830 0.89 -0.7 799,266 - IAI47_03830 0.89 +1.1 799,266 - IAI47_03830 0.89 +0.4 799,266 - IAI47_03830 0.89 +0.2 799,266 - IAI47_03830 0.89 -0.6 799,266 - IAI47_03830 0.89 -2.6 799,266 - IAI47_03830 0.89 +1.5 799,319 + +0.4 799,319 + -1.6 799,319 + +0.9 799,320 - +1.1 799,320 - -1.7 799,320 - -1.1 799,320 - +0.6 799,320 - -0.6 799,320 - -0.3 799,328 + -1.2 799,328 + -0.7 799,329 - -1.8 799,329 - +0.4 799,374 + -0.1 799,374 + +1.2 799,374 + -0.8 799,374 + +1.8 799,375 - +0.4 799,375 - -1.1 799,376 + +1.6 799,376 + +1.4 799,376 + -1.6 799,376 + +1.3 799,376 + -1.2 799,376 + +0.2 799,377 - -0.2 799,377 - +0.2 799,377 - -1.3 799,377 - +0.2 799,377 - -0.9 799,377 - -1.2 799,377 - -1.5 799,385 + -0.9 799,386 - -0.3 799,395 + +2.9 799,396 - -1.1 799,396 - -0.8 799,433 + +0.4 799,433 + +1.0 799,433 + -0.4 799,434 - -1.1 799,434 - +0.2 799,434 - +1.2 799,434 - -1.2 799,434 - -0.5 799,434 - +1.2 799,434 - +1.0 799,434 - +0.6 799,454 + +0.6 799,517 - +1.8 799,605 + -1.2 799,606 - +0.6 799,606 - +0.4 799,664 + +0.8 799,664 + +2.6 799,664 + -0.8 799,664 + +1.2 799,664 + +0.4 799,664 + +3.6
Or see this region's nucleotide sequence