Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_03545 and IAI47_03550 are separated by 971 nucleotides IAI47_03550 and IAI47_03555 are separated by 54 nucleotides IAI47_03555 and IAI47_03560 overlap by 1 nucleotides
IAI47_03545: IAI47_03545 - ornithine decarboxylase, at 739,628 to 741,781
_03545
IAI47_03550: IAI47_03550 - membrane-bound lytic murein transglycosylase MltC, at 742,753 to 743,832
_03550
IAI47_03555: IAI47_03555 - oxidative damage protection protein, at 743,887 to 744,159
_03555
IAI47_03560: IAI47_03560 - A/G-specific adenine glycosylase, at 744,159 to 745,244
_03560
Position (kb) 742
743
744 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 741.763 kb on + strand at 741.763 kb on + strand at 741.763 kb on + strand at 741.763 kb on + strand at 741.764 kb on - strand at 741.764 kb on - strand at 741.764 kb on - strand at 741.764 kb on - strand at 741.768 kb on + strand at 741.769 kb on - strand at 741.769 kb on - strand at 741.922 kb on + strand at 741.922 kb on + strand at 741.922 kb on + strand at 741.922 kb on + strand at 741.923 kb on - strand at 741.923 kb on - strand at 741.994 kb on - strand at 742.003 kb on - strand at 742.003 kb on - strand at 742.015 kb on + strand at 742.015 kb on + strand at 742.190 kb on + strand at 742.308 kb on + strand at 742.308 kb on + strand at 742.308 kb on + strand at 742.308 kb on + strand at 742.308 kb on + strand at 742.308 kb on + strand at 742.309 kb on - strand at 742.605 kb on + strand at 742.605 kb on + strand at 742.605 kb on + strand at 742.606 kb on - strand at 742.708 kb on + strand at 742.709 kb on - strand at 742.766 kb on + strand at 742.791 kb on + strand at 742.791 kb on + strand at 742.792 kb on - strand at 742.792 kb on - strand at 742.792 kb on - strand at 742.792 kb on - strand at 742.792 kb on - strand at 742.792 kb on - strand at 742.792 kb on - strand at 742.792 kb on - strand at 742.793 kb on + strand at 742.793 kb on + strand at 742.793 kb on + strand at 742.793 kb on + strand at 742.793 kb on + strand at 742.794 kb on - strand at 742.794 kb on - strand at 742.794 kb on - strand at 742.794 kb on - strand at 742.794 kb on - strand at 742.794 kb on - strand at 742.800 kb on - strand at 742.863 kb on + strand, within IAI47_03550 at 742.863 kb on + strand, within IAI47_03550 at 742.863 kb on + strand, within IAI47_03550 at 742.863 kb on + strand, within IAI47_03550 at 742.864 kb on - strand, within IAI47_03550 at 742.864 kb on - strand, within IAI47_03550 at 742.864 kb on - strand, within IAI47_03550 at 742.864 kb on - strand, within IAI47_03550 at 742.937 kb on + strand, within IAI47_03550 at 742.991 kb on + strand, within IAI47_03550 at 743.015 kb on + strand, within IAI47_03550 at 743.015 kb on + strand, within IAI47_03550 at 743.016 kb on - strand, within IAI47_03550 at 743.016 kb on - strand, within IAI47_03550 at 743.057 kb on + strand, within IAI47_03550 at 743.082 kb on + strand, within IAI47_03550 at 743.083 kb on - strand, within IAI47_03550 at 743.083 kb on - strand, within IAI47_03550 at 743.083 kb on - strand, within IAI47_03550 at 743.083 kb on - strand, within IAI47_03550 at 743.194 kb on - strand, within IAI47_03550 at 743.225 kb on + strand, within IAI47_03550 at 743.226 kb on - strand, within IAI47_03550 at 743.226 kb on - strand, within IAI47_03550 at 743.226 kb on - strand, within IAI47_03550 at 743.226 kb on - strand, within IAI47_03550 at 743.242 kb on - strand, within IAI47_03550 at 743.259 kb on - strand, within IAI47_03550 at 743.313 kb on - strand, within IAI47_03550 at 743.313 kb on - strand, within IAI47_03550 at 743.349 kb on + strand, within IAI47_03550 at 743.349 kb on + strand, within IAI47_03550 at 743.350 kb on - strand, within IAI47_03550 at 743.350 kb on - strand, within IAI47_03550 at 743.350 kb on - strand, within IAI47_03550 at 743.350 kb on - strand, within IAI47_03550 at 743.350 kb on - strand, within IAI47_03550 at 743.363 kb on + strand, within IAI47_03550 at 743.363 kb on + strand, within IAI47_03550 at 743.363 kb on + strand, within IAI47_03550 at 743.363 kb on + strand, within IAI47_03550 at 743.363 kb on + strand, within IAI47_03550 at 743.363 kb on + strand, within IAI47_03550 at 743.364 kb on - strand, within IAI47_03550 at 743.432 kb on + strand, within IAI47_03550 at 743.432 kb on + strand, within IAI47_03550 at 743.433 kb on - strand, within IAI47_03550 at 743.433 kb on - strand, within IAI47_03550 at 743.433 kb on - strand, within IAI47_03550 at 743.448 kb on + strand, within IAI47_03550 at 743.477 kb on + strand, within IAI47_03550 at 743.477 kb on + strand, within IAI47_03550 at 743.478 kb on - strand, within IAI47_03550 at 743.584 kb on - strand, within IAI47_03550 at 743.584 kb on - strand, within IAI47_03550 at 743.615 kb on + strand, within IAI47_03550 at 743.615 kb on + strand, within IAI47_03550 at 743.616 kb on - strand, within IAI47_03550 at 743.616 kb on - strand, within IAI47_03550 at 743.618 kb on + strand, within IAI47_03550 at 743.618 kb on + strand, within IAI47_03550 at 743.618 kb on + strand, within IAI47_03550 at 743.618 kb on + strand, within IAI47_03550 at 743.618 kb on + strand, within IAI47_03550 at 743.618 kb on + strand, within IAI47_03550 at 743.618 kb on + strand, within IAI47_03550 at 743.618 kb on + strand, within IAI47_03550 at 743.618 kb on + strand, within IAI47_03550 at 743.618 kb on + strand, within IAI47_03550 at 743.619 kb on - strand, within IAI47_03550 at 743.619 kb on - strand, within IAI47_03550 at 743.619 kb on - strand, within IAI47_03550 at 743.630 kb on + strand, within IAI47_03550 at 743.630 kb on + strand, within IAI47_03550 at 743.639 kb on + strand, within IAI47_03550 at 743.683 kb on - strand, within IAI47_03550 at 743.692 kb on - strand, within IAI47_03550 at 743.692 kb on - strand, within IAI47_03550 at 743.694 kb on + strand, within IAI47_03550 at 743.694 kb on + strand, within IAI47_03550 at 743.694 kb on + strand, within IAI47_03550 at 743.695 kb on - strand, within IAI47_03550 at 743.695 kb on - strand, within IAI47_03550 at 743.695 kb on - strand, within IAI47_03550 at 743.695 kb on - strand, within IAI47_03550 at 743.740 kb on + strand at 743.800 kb on + strand at 743.800 kb on + strand at 743.801 kb on - strand at 743.801 kb on - strand at 743.801 kb on - strand at 743.801 kb on - strand at 743.889 kb on - strand at 743.889 kb on - strand at 743.904 kb on + strand at 743.904 kb on + strand at 743.904 kb on + strand at 743.904 kb on + strand at 743.904 kb on + strand at 743.904 kb on + strand at 743.904 kb on + strand at 743.904 kb on + strand at 743.904 kb on + strand at 743.905 kb on - strand at 743.905 kb on - strand at 743.905 kb on - strand at 743.905 kb on - strand at 743.905 kb on - strand at 743.905 kb on - strand at 743.905 kb on - strand at 743.905 kb on - strand at 743.905 kb on - strand at 743.905 kb on - strand at 743.906 kb on + strand at 743.907 kb on - strand at 743.907 kb on - strand at 743.907 kb on - strand at 743.916 kb on + strand, within IAI47_03555 at 743.916 kb on + strand, within IAI47_03555 at 743.917 kb on - strand, within IAI47_03555 at 743.917 kb on - strand, within IAI47_03555 at 743.917 kb on - strand, within IAI47_03555 at 743.928 kb on + strand, within IAI47_03555 at 743.928 kb on + strand, within IAI47_03555 at 743.928 kb on + strand, within IAI47_03555 at 743.928 kb on + strand, within IAI47_03555 at 743.928 kb on + strand, within IAI47_03555 at 743.929 kb on - strand, within IAI47_03555 at 743.929 kb on - strand, within IAI47_03555 at 743.929 kb on - strand, within IAI47_03555 at 743.929 kb on - strand, within IAI47_03555 at 743.970 kb on + strand, within IAI47_03555 at 743.970 kb on + strand, within IAI47_03555 at 743.970 kb on + strand, within IAI47_03555 at 743.970 kb on + strand, within IAI47_03555 at 743.971 kb on - strand, within IAI47_03555 at 743.971 kb on - strand, within IAI47_03555 at 743.971 kb on - strand, within IAI47_03555 at 744.059 kb on - strand, within IAI47_03555 at 744.068 kb on + strand, within IAI47_03555 at 744.068 kb on + strand, within IAI47_03555 at 744.068 kb on + strand, within IAI47_03555 at 744.068 kb on + strand, within IAI47_03555 at 744.069 kb on - strand, within IAI47_03555 at 744.069 kb on - strand, within IAI47_03555 at 744.069 kb on - strand, within IAI47_03555 at 744.072 kb on + strand, within IAI47_03555 at 744.072 kb on + strand, within IAI47_03555 at 744.073 kb on - strand, within IAI47_03555 at 744.073 kb on - strand, within IAI47_03555 at 744.073 kb on - strand, within IAI47_03555 at 744.078 kb on + strand, within IAI47_03555 at 744.160 kb on + strand at 744.160 kb on + strand at 744.160 kb on + strand at 744.160 kb on + strand at 744.161 kb on - strand at 744.173 kb on + strand at 744.174 kb on - strand at 744.222 kb on + strand at 744.222 kb on + strand at 744.223 kb on - strand at 744.223 kb on - strand at 744.247 kb on - strand at 744.247 kb on - strand at 744.270 kb on + strand, within IAI47_03560 at 744.271 kb on - strand, within IAI47_03560 at 744.276 kb on - strand, within IAI47_03560 at 744.276 kb on - strand, within IAI47_03560 at 744.277 kb on + strand, within IAI47_03560 at 744.278 kb on - strand, within IAI47_03560 at 744.278 kb on - strand, within IAI47_03560 at 744.278 kb on - strand, within IAI47_03560 at 744.363 kb on + strand, within IAI47_03560 at 744.569 kb on + strand, within IAI47_03560 at 744.569 kb on + strand, within IAI47_03560 at 744.570 kb on - strand, within IAI47_03560 at 744.570 kb on - strand, within IAI47_03560 at 744.586 kb on + strand, within IAI47_03560 at 744.610 kb on + strand, within IAI47_03560 at 744.611 kb on - strand, within IAI47_03560 at 744.611 kb on - strand, within IAI47_03560 at 744.629 kb on + strand, within IAI47_03560 at 744.630 kb on - strand, within IAI47_03560 at 744.630 kb on - strand, within IAI47_03560 at 744.630 kb on - strand, within IAI47_03560 at 744.630 kb on - strand, within IAI47_03560 at 744.701 kb on + strand, within IAI47_03560 at 744.702 kb on - strand, within IAI47_03560 at 744.797 kb on - strand, within IAI47_03560
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 741,763 + +2.3 741,763 + +0.6 741,763 + +0.7 741,763 + +1.7 741,764 - -2.4 741,764 - +2.1 741,764 - -0.3 741,764 - -2.5 741,768 + +1.8 741,769 - +1.1 741,769 - +1.2 741,922 + +0.2 741,922 + -0.1 741,922 + -1.0 741,922 + -1.4 741,923 - +0.2 741,923 - +0.9 741,994 - +0.6 742,003 - +1.1 742,003 - +1.5 742,015 + -0.6 742,015 + -2.5 742,190 + -0.2 742,308 + +0.7 742,308 + +1.5 742,308 + +0.8 742,308 + +1.2 742,308 + -1.0 742,308 + +1.1 742,309 - -1.2 742,605 + +0.0 742,605 + +0.5 742,605 + -0.5 742,606 - +0.6 742,708 + +0.2 742,709 - +0.4 742,766 + +1.2 742,791 + -0.7 742,791 + -2.2 742,792 - -2.0 742,792 - -0.9 742,792 - -1.2 742,792 - -0.8 742,792 - -0.0 742,792 - -2.2 742,792 - -2.3 742,792 - -2.3 742,793 + -0.7 742,793 + +1.6 742,793 + -0.9 742,793 + -0.8 742,793 + -2.1 742,794 - -2.5 742,794 - -1.3 742,794 - -1.8 742,794 - -0.4 742,794 - -2.5 742,794 - -1.8 742,800 - -3.2 742,863 + IAI47_03550 0.10 -2.1 742,863 + IAI47_03550 0.10 -2.1 742,863 + IAI47_03550 0.10 -1.9 742,863 + IAI47_03550 0.10 +0.1 742,864 - IAI47_03550 0.10 -2.9 742,864 - IAI47_03550 0.10 -1.5 742,864 - IAI47_03550 0.10 +0.2 742,864 - IAI47_03550 0.10 +0.7 742,937 + IAI47_03550 0.17 -2.1 742,991 + IAI47_03550 0.22 -1.7 743,015 + IAI47_03550 0.24 -0.5 743,015 + IAI47_03550 0.24 -2.8 743,016 - IAI47_03550 0.24 -2.3 743,016 - IAI47_03550 0.24 -2.0 743,057 + IAI47_03550 0.28 -0.0 743,082 + IAI47_03550 0.30 +0.2 743,083 - IAI47_03550 0.31 -1.2 743,083 - IAI47_03550 0.31 -1.7 743,083 - IAI47_03550 0.31 -1.5 743,083 - IAI47_03550 0.31 -2.0 743,194 - IAI47_03550 0.41 -3.9 743,225 + IAI47_03550 0.44 -2.6 743,226 - IAI47_03550 0.44 -2.0 743,226 - IAI47_03550 0.44 -1.0 743,226 - IAI47_03550 0.44 -1.6 743,226 - IAI47_03550 0.44 +0.3 743,242 - IAI47_03550 0.45 -0.7 743,259 - IAI47_03550 0.47 -3.4 743,313 - IAI47_03550 0.52 -2.3 743,313 - IAI47_03550 0.52 +2.8 743,349 + IAI47_03550 0.55 -3.1 743,349 + IAI47_03550 0.55 -2.5 743,350 - IAI47_03550 0.55 -2.4 743,350 - IAI47_03550 0.55 +0.2 743,350 - IAI47_03550 0.55 +1.4 743,350 - IAI47_03550 0.55 -1.0 743,350 - IAI47_03550 0.55 -2.5 743,363 + IAI47_03550 0.56 -1.9 743,363 + IAI47_03550 0.56 +0.2 743,363 + IAI47_03550 0.56 -2.1 743,363 + IAI47_03550 0.56 -2.8 743,363 + IAI47_03550 0.56 -1.6 743,363 + IAI47_03550 0.56 +0.7 743,364 - IAI47_03550 0.57 -0.9 743,432 + IAI47_03550 0.63 -0.9 743,432 + IAI47_03550 0.63 -2.0 743,433 - IAI47_03550 0.63 -1.8 743,433 - IAI47_03550 0.63 -1.6 743,433 - IAI47_03550 0.63 -0.7 743,448 + IAI47_03550 0.64 -0.1 743,477 + IAI47_03550 0.67 -1.3 743,477 + IAI47_03550 0.67 -1.8 743,478 - IAI47_03550 0.67 -2.0 743,584 - IAI47_03550 0.77 -1.4 743,584 - IAI47_03550 0.77 -2.5 743,615 + IAI47_03550 0.80 -1.7 743,615 + IAI47_03550 0.80 -2.9 743,616 - IAI47_03550 0.80 -1.8 743,616 - IAI47_03550 0.80 -2.3 743,618 + IAI47_03550 0.80 -3.6 743,618 + IAI47_03550 0.80 -0.7 743,618 + IAI47_03550 0.80 -2.4 743,618 + IAI47_03550 0.80 -1.3 743,618 + IAI47_03550 0.80 -2.4 743,618 + IAI47_03550 0.80 -2.2 743,618 + IAI47_03550 0.80 -1.0 743,618 + IAI47_03550 0.80 -2.8 743,618 + IAI47_03550 0.80 -1.7 743,618 + IAI47_03550 0.80 -1.3 743,619 - IAI47_03550 0.80 +1.6 743,619 - IAI47_03550 0.80 -2.5 743,619 - IAI47_03550 0.80 -1.7 743,630 + IAI47_03550 0.81 -1.2 743,630 + IAI47_03550 0.81 -2.2 743,639 + IAI47_03550 0.82 -2.2 743,683 - IAI47_03550 0.86 -3.0 743,692 - IAI47_03550 0.87 -0.7 743,692 - IAI47_03550 0.87 -2.4 743,694 + IAI47_03550 0.87 -2.3 743,694 + IAI47_03550 0.87 -0.7 743,694 + IAI47_03550 0.87 -2.2 743,695 - IAI47_03550 0.87 -1.8 743,695 - IAI47_03550 0.87 -2.7 743,695 - IAI47_03550 0.87 -0.9 743,695 - IAI47_03550 0.87 -3.2 743,740 + -2.2 743,800 + -2.7 743,800 + -2.1 743,801 - +1.2 743,801 - -2.2 743,801 - -4.6 743,801 - -1.1 743,889 - +0.8 743,889 - +1.0 743,904 + -2.6 743,904 + -1.7 743,904 + -0.6 743,904 + -0.2 743,904 + +1.3 743,904 + -1.0 743,904 + -2.1 743,904 + -1.9 743,904 + -2.1 743,905 - +0.3 743,905 - -0.2 743,905 - -2.1 743,905 - +1.8 743,905 - +0.0 743,905 - -0.9 743,905 - +1.5 743,905 - +1.2 743,905 - -0.2 743,905 - -2.1 743,906 + -1.1 743,907 - -1.1 743,907 - +1.4 743,907 - +0.7 743,916 + IAI47_03555 0.11 -2.2 743,916 + IAI47_03555 0.11 -2.2 743,917 - IAI47_03555 0.11 +0.5 743,917 - IAI47_03555 0.11 +1.6 743,917 - IAI47_03555 0.11 -0.0 743,928 + IAI47_03555 0.15 -0.0 743,928 + IAI47_03555 0.15 -2.2 743,928 + IAI47_03555 0.15 -1.7 743,928 + IAI47_03555 0.15 +0.7 743,928 + IAI47_03555 0.15 -3.0 743,929 - IAI47_03555 0.15 +1.1 743,929 - IAI47_03555 0.15 -0.0 743,929 - IAI47_03555 0.15 -0.3 743,929 - IAI47_03555 0.15 -1.2 743,970 + IAI47_03555 0.30 -0.2 743,970 + IAI47_03555 0.30 -1.8 743,970 + IAI47_03555 0.30 -1.5 743,970 + IAI47_03555 0.30 -0.8 743,971 - IAI47_03555 0.31 -2.2 743,971 - IAI47_03555 0.31 +1.1 743,971 - IAI47_03555 0.31 -0.3 744,059 - IAI47_03555 0.63 +0.5 744,068 + IAI47_03555 0.66 +1.8 744,068 + IAI47_03555 0.66 +0.1 744,068 + IAI47_03555 0.66 -0.9 744,068 + IAI47_03555 0.66 -0.8 744,069 - IAI47_03555 0.67 +1.3 744,069 - IAI47_03555 0.67 +1.1 744,069 - IAI47_03555 0.67 +2.4 744,072 + IAI47_03555 0.68 +1.2 744,072 + IAI47_03555 0.68 +1.3 744,073 - IAI47_03555 0.68 -0.7 744,073 - IAI47_03555 0.68 -0.4 744,073 - IAI47_03555 0.68 +1.8 744,078 + IAI47_03555 0.70 +2.3 744,160 + -1.8 744,160 + -0.9 744,160 + -0.6 744,160 + +1.8 744,161 - -1.0 744,173 + -1.6 744,174 - +2.5 744,222 + -2.1 744,222 + +2.9 744,223 - -2.2 744,223 - -2.4 744,247 - +0.2 744,247 - +1.1 744,270 + IAI47_03560 0.10 +1.9 744,271 - IAI47_03560 0.10 +1.6 744,276 - IAI47_03560 0.11 +2.1 744,276 - IAI47_03560 0.11 +0.4 744,277 + IAI47_03560 0.11 -1.5 744,278 - IAI47_03560 0.11 +0.3 744,278 - IAI47_03560 0.11 -0.3 744,278 - IAI47_03560 0.11 +1.3 744,363 + IAI47_03560 0.19 -2.1 744,569 + IAI47_03560 0.38 -1.7 744,569 + IAI47_03560 0.38 -1.2 744,570 - IAI47_03560 0.38 +0.2 744,570 - IAI47_03560 0.38 -0.6 744,586 + IAI47_03560 0.39 +0.2 744,610 + IAI47_03560 0.42 -3.0 744,611 - IAI47_03560 0.42 -2.7 744,611 - IAI47_03560 0.42 +0.3 744,629 + IAI47_03560 0.43 -0.1 744,630 - IAI47_03560 0.43 +0.7 744,630 - IAI47_03560 0.43 +0.3 744,630 - IAI47_03560 0.43 +0.6 744,630 - IAI47_03560 0.43 +0.5 744,701 + IAI47_03560 0.50 +0.1 744,702 - IAI47_03560 0.50 -3.3 744,797 - IAI47_03560 0.59 +0.2
Or see this region's nucleotide sequence