Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19325 and IAI47_19330 are separated by 506 nucleotides IAI47_19330 and IAI47_19335 are separated by 282 nucleotides
IAI47_19325: IAI47_19325 - transposase, at 89,969 to 90,253
_19325
IAI47_19330: IAI47_19330 - hypothetical protein, at 90,760 to 91,107
_19330
IAI47_19335: IAI47_19335 - succinyldiaminopimelate transaminase, at 91,390 to 92,592
_19335
Position (kb) 90
91
92 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 89.864 kb on + strand at 89.865 kb on - strand at 89.866 kb on + strand at 89.866 kb on + strand at 89.867 kb on - strand at 89.910 kb on + strand at 89.910 kb on + strand at 89.915 kb on - strand at 89.926 kb on - strand at 89.939 kb on + strand at 89.940 kb on - strand at 89.957 kb on + strand at 89.959 kb on + strand at 89.960 kb on - strand at 89.960 kb on - strand at 89.960 kb on - strand at 90.005 kb on + strand, within IAI47_19325 at 90.005 kb on + strand, within IAI47_19325 at 90.049 kb on + strand, within IAI47_19325 at 90.049 kb on + strand, within IAI47_19325 at 90.049 kb on + strand, within IAI47_19325 at 90.049 kb on + strand, within IAI47_19325 at 90.049 kb on + strand, within IAI47_19325 at 90.049 kb on + strand, within IAI47_19325 at 90.050 kb on - strand, within IAI47_19325 at 90.050 kb on - strand, within IAI47_19325 at 90.090 kb on - strand, within IAI47_19325 at 90.142 kb on + strand, within IAI47_19325 at 90.245 kb on + strand at 90.245 kb on + strand at 90.284 kb on + strand at 90.284 kb on + strand at 90.284 kb on + strand at 90.284 kb on + strand at 90.284 kb on + strand at 90.284 kb on + strand at 90.285 kb on - strand at 90.285 kb on - strand at 90.285 kb on - strand at 90.285 kb on - strand at 90.285 kb on - strand at 90.287 kb on + strand at 90.288 kb on - strand at 90.298 kb on - strand at 90.298 kb on - strand at 90.301 kb on + strand at 90.301 kb on + strand at 90.301 kb on + strand at 90.327 kb on + strand at 90.328 kb on - strand at 90.402 kb on + strand at 90.402 kb on + strand at 90.403 kb on - strand at 90.403 kb on - strand at 90.419 kb on + strand at 90.419 kb on + strand at 90.489 kb on + strand at 90.490 kb on - strand at 90.530 kb on - strand at 90.557 kb on + strand at 90.601 kb on + strand at 90.695 kb on + strand at 90.696 kb on - strand at 90.696 kb on - strand at 90.719 kb on + strand at 90.719 kb on + strand at 90.720 kb on - strand at 90.726 kb on + strand at 90.726 kb on + strand at 90.726 kb on + strand at 90.726 kb on + strand at 90.727 kb on - strand at 90.727 kb on - strand at 90.727 kb on - strand at 90.727 kb on - strand at 90.727 kb on - strand at 90.727 kb on - strand at 90.727 kb on - strand at 90.727 kb on - strand at 90.727 kb on - strand at 90.775 kb on - strand at 90.800 kb on + strand, within IAI47_19330 at 90.800 kb on + strand, within IAI47_19330 at 90.801 kb on - strand, within IAI47_19330 at 90.825 kb on + strand, within IAI47_19330 at 90.825 kb on + strand, within IAI47_19330 at 90.826 kb on - strand, within IAI47_19330 at 90.884 kb on + strand, within IAI47_19330 at 90.897 kb on + strand, within IAI47_19330 at 90.898 kb on - strand, within IAI47_19330 at 90.951 kb on + strand, within IAI47_19330 at 90.951 kb on + strand, within IAI47_19330 at 90.952 kb on - strand, within IAI47_19330 at 90.952 kb on - strand, within IAI47_19330 at 90.968 kb on + strand, within IAI47_19330 at 90.969 kb on - strand, within IAI47_19330 at 90.975 kb on + strand, within IAI47_19330 at 90.975 kb on + strand, within IAI47_19330 at 90.976 kb on - strand, within IAI47_19330 at 90.993 kb on + strand, within IAI47_19330 at 90.993 kb on + strand, within IAI47_19330 at 90.993 kb on + strand, within IAI47_19330 at 91.006 kb on + strand, within IAI47_19330 at 91.007 kb on - strand, within IAI47_19330 at 91.007 kb on - strand, within IAI47_19330 at 91.007 kb on - strand, within IAI47_19330 at 91.007 kb on - strand, within IAI47_19330 at 91.032 kb on + strand, within IAI47_19330 at 91.032 kb on + strand, within IAI47_19330 at 91.043 kb on + strand, within IAI47_19330 at 91.052 kb on - strand, within IAI47_19330 at 91.106 kb on - strand at 91.126 kb on + strand at 91.217 kb on - strand at 91.225 kb on + strand at 91.282 kb on - strand at 91.282 kb on - strand at 91.309 kb on + strand at 91.309 kb on + strand at 91.310 kb on - strand at 91.310 kb on - strand at 91.315 kb on + strand at 91.316 kb on - strand at 91.325 kb on + strand at 91.325 kb on + strand at 91.362 kb on + strand at 91.363 kb on - strand at 91.369 kb on + strand at 91.370 kb on - strand at 91.370 kb on - strand at 91.374 kb on + strand at 91.374 kb on + strand at 91.384 kb on - strand at 91.384 kb on - strand at 91.392 kb on - strand at 91.392 kb on - strand at 91.395 kb on + strand at 91.395 kb on + strand at 91.396 kb on - strand at 91.400 kb on + strand at 91.400 kb on + strand at 91.400 kb on + strand at 91.400 kb on + strand at 91.400 kb on + strand at 91.401 kb on - strand at 91.401 kb on - strand at 91.401 kb on - strand at 91.405 kb on - strand at 91.462 kb on + strand at 91.462 kb on + strand at 91.463 kb on - strand at 91.463 kb on - strand at 91.463 kb on - strand at 91.463 kb on - strand at 91.463 kb on - strand at 91.463 kb on - strand at 91.463 kb on - strand at 91.520 kb on + strand, within IAI47_19335 at 91.521 kb on - strand, within IAI47_19335 at 91.521 kb on - strand, within IAI47_19335 at 91.521 kb on - strand, within IAI47_19335 at 91.521 kb on - strand, within IAI47_19335 at 91.521 kb on - strand, within IAI47_19335 at 91.539 kb on + strand, within IAI47_19335 at 91.540 kb on - strand, within IAI47_19335 at 91.549 kb on - strand, within IAI47_19335 at 91.555 kb on - strand, within IAI47_19335 at 91.607 kb on + strand, within IAI47_19335 at 91.607 kb on + strand, within IAI47_19335 at 91.607 kb on + strand, within IAI47_19335 at 91.607 kb on + strand, within IAI47_19335 at 91.608 kb on - strand, within IAI47_19335 at 91.636 kb on - strand, within IAI47_19335 at 91.636 kb on - strand, within IAI47_19335 at 91.636 kb on - strand, within IAI47_19335 at 91.746 kb on + strand, within IAI47_19335 at 91.747 kb on - strand, within IAI47_19335 at 91.747 kb on - strand, within IAI47_19335 at 91.772 kb on + strand, within IAI47_19335 at 91.772 kb on + strand, within IAI47_19335 at 91.772 kb on + strand, within IAI47_19335 at 91.772 kb on + strand, within IAI47_19335 at 91.772 kb on + strand, within IAI47_19335 at 91.772 kb on + strand, within IAI47_19335 at 91.772 kb on + strand, within IAI47_19335 at 91.772 kb on + strand, within IAI47_19335 at 91.772 kb on + strand, within IAI47_19335 at 91.772 kb on + strand, within IAI47_19335 at 91.773 kb on - strand, within IAI47_19335 at 91.773 kb on - strand, within IAI47_19335 at 91.773 kb on - strand, within IAI47_19335 at 91.773 kb on - strand, within IAI47_19335 at 91.888 kb on - strand, within IAI47_19335 at 91.924 kb on + strand, within IAI47_19335 at 91.925 kb on - strand, within IAI47_19335 at 91.967 kb on + strand, within IAI47_19335 at 91.968 kb on - strand, within IAI47_19335 at 91.989 kb on + strand, within IAI47_19335 at 91.989 kb on + strand, within IAI47_19335 at 91.989 kb on + strand, within IAI47_19335 at 91.990 kb on - strand, within IAI47_19335 at 91.990 kb on - strand, within IAI47_19335 at 91.990 kb on - strand, within IAI47_19335 at 91.997 kb on + strand, within IAI47_19335 at 92.001 kb on + strand, within IAI47_19335 at 92.002 kb on - strand, within IAI47_19335 at 92.013 kb on + strand, within IAI47_19335 at 92.014 kb on - strand, within IAI47_19335 at 92.041 kb on + strand, within IAI47_19335 at 92.043 kb on + strand, within IAI47_19335 at 92.044 kb on - strand, within IAI47_19335 at 92.044 kb on - strand, within IAI47_19335 at 92.044 kb on - strand, within IAI47_19335 at 92.056 kb on - strand, within IAI47_19335 at 92.080 kb on + strand, within IAI47_19335 at 92.081 kb on - strand, within IAI47_19335 at 92.081 kb on - strand, within IAI47_19335 at 92.081 kb on - strand, within IAI47_19335 at 92.081 kb on - strand, within IAI47_19335 at 92.104 kb on - strand, within IAI47_19335 at 92.104 kb on - strand, within IAI47_19335
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 89,864 + -0.3 89,865 - -1.4 89,866 + +0.7 89,866 + +0.0 89,867 - +1.0 89,910 + -1.4 89,910 + -0.6 89,915 - -2.3 89,926 - -0.8 89,939 + -1.7 89,940 - +2.2 89,957 + -3.1 89,959 + -1.1 89,960 - -2.6 89,960 - -0.4 89,960 - -0.5 90,005 + IAI47_19325 0.13 +1.2 90,005 + IAI47_19325 0.13 -2.2 90,049 + IAI47_19325 0.28 -0.3 90,049 + IAI47_19325 0.28 -1.6 90,049 + IAI47_19325 0.28 -0.3 90,049 + IAI47_19325 0.28 +1.6 90,049 + IAI47_19325 0.28 -0.6 90,049 + IAI47_19325 0.28 -0.3 90,050 - IAI47_19325 0.28 +1.5 90,050 - IAI47_19325 0.28 +0.0 90,090 - IAI47_19325 0.42 +1.2 90,142 + IAI47_19325 0.61 +0.4 90,245 + -0.5 90,245 + +0.5 90,284 + +0.9 90,284 + +2.0 90,284 + -0.1 90,284 + +0.1 90,284 + -1.1 90,284 + +1.3 90,285 - +0.2 90,285 - -0.1 90,285 - -0.9 90,285 - +0.6 90,285 - +0.9 90,287 + +0.4 90,288 - -0.9 90,298 - +2.2 90,298 - -1.9 90,301 + +0.3 90,301 + +0.7 90,301 + +0.0 90,327 + +1.1 90,328 - -1.1 90,402 + +0.8 90,402 + +1.8 90,403 - +0.2 90,403 - +1.3 90,419 + +2.8 90,419 + -0.8 90,489 + +0.7 90,490 - +0.6 90,530 - -1.0 90,557 + -1.5 90,601 + -0.1 90,695 + -0.0 90,696 - -0.3 90,696 - -0.7 90,719 + +0.3 90,719 + -1.9 90,720 - +2.5 90,726 + +2.2 90,726 + +0.3 90,726 + +1.4 90,726 + +1.2 90,727 - -2.5 90,727 - +2.4 90,727 - +0.5 90,727 - +1.0 90,727 - -0.8 90,727 - -0.1 90,727 - +0.5 90,727 - +0.6 90,727 - +0.3 90,775 - -0.5 90,800 + IAI47_19330 0.11 +3.1 90,800 + IAI47_19330 0.11 +0.8 90,801 - IAI47_19330 0.12 +2.3 90,825 + IAI47_19330 0.19 -1.1 90,825 + IAI47_19330 0.19 +0.8 90,826 - IAI47_19330 0.19 +3.1 90,884 + IAI47_19330 0.36 +1.7 90,897 + IAI47_19330 0.39 +0.2 90,898 - IAI47_19330 0.40 +0.2 90,951 + IAI47_19330 0.55 +2.2 90,951 + IAI47_19330 0.55 +2.1 90,952 - IAI47_19330 0.55 -0.8 90,952 - IAI47_19330 0.55 -0.7 90,968 + IAI47_19330 0.60 +2.3 90,969 - IAI47_19330 0.60 +1.4 90,975 + IAI47_19330 0.62 -0.3 90,975 + IAI47_19330 0.62 +1.5 90,976 - IAI47_19330 0.62 +1.5 90,993 + IAI47_19330 0.67 -1.4 90,993 + IAI47_19330 0.67 -0.3 90,993 + IAI47_19330 0.67 -2.5 91,006 + IAI47_19330 0.71 -1.7 91,007 - IAI47_19330 0.71 -2.1 91,007 - IAI47_19330 0.71 +0.4 91,007 - IAI47_19330 0.71 -0.0 91,007 - IAI47_19330 0.71 +0.3 91,032 + IAI47_19330 0.78 +0.7 91,032 + IAI47_19330 0.78 +0.2 91,043 + IAI47_19330 0.81 +0.9 91,052 - IAI47_19330 0.84 +0.2 91,106 - -0.8 91,126 + +0.9 91,217 - -1.5 91,225 + -1.3 91,282 - -0.2 91,282 - +1.2 91,309 + -0.1 91,309 + +2.2 91,310 - +1.3 91,310 - +1.6 91,315 + +1.0 91,316 - +0.6 91,325 + +1.0 91,325 + +1.9 91,362 + +1.8 91,363 - +1.1 91,369 + -1.0 91,370 - -0.1 91,370 - +1.0 91,374 + -2.3 91,374 + -2.1 91,384 - +1.9 91,384 - +1.2 91,392 - +1.3 91,392 - +0.4 91,395 + +0.1 91,395 + -1.8 91,396 - +0.5 91,400 + -0.9 91,400 + +0.9 91,400 + +0.7 91,400 + -1.0 91,400 + +0.3 91,401 - +1.2 91,401 - -0.2 91,401 - -1.0 91,405 - -0.1 91,462 + -0.3 91,462 + +0.1 91,463 - +1.3 91,463 - -2.4 91,463 - -0.1 91,463 - +1.3 91,463 - +0.9 91,463 - +0.3 91,463 - -0.6 91,520 + IAI47_19335 0.11 -2.0 91,521 - IAI47_19335 0.11 +0.3 91,521 - IAI47_19335 0.11 +0.8 91,521 - IAI47_19335 0.11 -2.5 91,521 - IAI47_19335 0.11 -1.5 91,521 - IAI47_19335 0.11 +1.5 91,539 + IAI47_19335 0.12 +0.7 91,540 - IAI47_19335 0.12 -1.3 91,549 - IAI47_19335 0.13 -0.3 91,555 - IAI47_19335 0.14 +0.3 91,607 + IAI47_19335 0.18 +1.2 91,607 + IAI47_19335 0.18 -1.2 91,607 + IAI47_19335 0.18 -0.5 91,607 + IAI47_19335 0.18 +2.3 91,608 - IAI47_19335 0.18 +0.8 91,636 - IAI47_19335 0.20 +0.8 91,636 - IAI47_19335 0.20 -0.3 91,636 - IAI47_19335 0.20 -0.5 91,746 + IAI47_19335 0.30 -0.2 91,747 - IAI47_19335 0.30 +0.8 91,747 - IAI47_19335 0.30 +0.2 91,772 + IAI47_19335 0.32 +1.6 91,772 + IAI47_19335 0.32 +0.5 91,772 + IAI47_19335 0.32 +3.9 91,772 + IAI47_19335 0.32 +1.2 91,772 + IAI47_19335 0.32 +1.4 91,772 + IAI47_19335 0.32 +3.5 91,772 + IAI47_19335 0.32 +1.1 91,772 + IAI47_19335 0.32 -0.6 91,772 + IAI47_19335 0.32 -1.9 91,772 + IAI47_19335 0.32 +1.4 91,773 - IAI47_19335 0.32 -0.8 91,773 - IAI47_19335 0.32 -2.5 91,773 - IAI47_19335 0.32 -0.0 91,773 - IAI47_19335 0.32 +0.6 91,888 - IAI47_19335 0.41 +1.3 91,924 + IAI47_19335 0.44 +2.2 91,925 - IAI47_19335 0.44 -0.6 91,967 + IAI47_19335 0.48 -0.4 91,968 - IAI47_19335 0.48 +1.0 91,989 + IAI47_19335 0.50 -1.6 91,989 + IAI47_19335 0.50 -0.1 91,989 + IAI47_19335 0.50 +1.6 91,990 - IAI47_19335 0.50 -2.6 91,990 - IAI47_19335 0.50 +0.0 91,990 - IAI47_19335 0.50 -0.3 91,997 + IAI47_19335 0.50 -0.9 92,001 + IAI47_19335 0.51 +0.2 92,002 - IAI47_19335 0.51 +0.4 92,013 + IAI47_19335 0.52 +1.2 92,014 - IAI47_19335 0.52 -0.5 92,041 + IAI47_19335 0.54 +0.3 92,043 + IAI47_19335 0.54 -1.3 92,044 - IAI47_19335 0.54 +1.0 92,044 - IAI47_19335 0.54 +2.0 92,044 - IAI47_19335 0.54 +1.4 92,056 - IAI47_19335 0.55 -0.2 92,080 + IAI47_19335 0.57 -0.2 92,081 - IAI47_19335 0.57 -1.8 92,081 - IAI47_19335 0.57 -0.9 92,081 - IAI47_19335 0.57 -1.0 92,081 - IAI47_19335 0.57 +2.0 92,104 - IAI47_19335 0.59 +2.0 92,104 - IAI47_19335 0.59 +1.2
Or see this region's nucleotide sequence