Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_18395 and IAI47_18400 overlap by 4 nucleotides IAI47_18400 and IAI47_18405 overlap by 4 nucleotides IAI47_18405 and IAI47_18410 overlap by 4 nucleotides IAI47_18410 and IAI47_18415 are separated by 28 nucleotides
IAI47_18395: IAI47_18395 - 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YigB, at 3,916,045 to 3,916,761
_18395
IAI47_18400: IAI47_18400 - tyrosine recombinase XerC, at 3,916,758 to 3,917,663
_18400
IAI47_18405: IAI47_18405 - DUF484 domain-containing protein, at 3,917,660 to 3,918,364
_18405
IAI47_18410: IAI47_18410 - diaminopimelate epimerase, at 3,918,361 to 3,919,185
_18410
IAI47_18415: IAI47_18415 - lipoprotein, at 3,919,214 to 3,919,414
_18415
Position (kb)
3917
3918
3919 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3916.671 kb on + strand, within IAI47_18395 at 3916.671 kb on + strand, within IAI47_18395 at 3916.688 kb on + strand, within IAI47_18395 at 3916.688 kb on + strand at 3916.688 kb on + strand, within IAI47_18395 at 3916.689 kb on - strand, within IAI47_18395 at 3916.692 kb on + strand at 3916.692 kb on + strand at 3916.692 kb on + strand at 3916.692 kb on + strand at 3916.693 kb on - strand at 3916.693 kb on - strand at 3916.693 kb on - strand at 3916.697 kb on + strand at 3916.697 kb on + strand at 3916.698 kb on - strand at 3916.725 kb on + strand at 3916.725 kb on + strand at 3916.726 kb on - strand at 3916.726 kb on - strand at 3916.726 kb on - strand at 3916.726 kb on - strand at 3916.732 kb on + strand at 3916.732 kb on + strand at 3916.733 kb on - strand at 3916.733 kb on - strand at 3916.733 kb on - strand at 3916.752 kb on - strand at 3916.752 kb on - strand at 3916.838 kb on - strand at 3916.872 kb on + strand, within IAI47_18400 at 3917.002 kb on - strand, within IAI47_18400 at 3917.002 kb on - strand, within IAI47_18400 at 3917.234 kb on - strand, within IAI47_18400 at 3917.234 kb on - strand, within IAI47_18400 at 3917.234 kb on - strand, within IAI47_18400 at 3917.345 kb on + strand, within IAI47_18400 at 3917.346 kb on - strand, within IAI47_18400 at 3917.358 kb on + strand, within IAI47_18400 at 3917.575 kb on + strand at 3917.658 kb on - strand at 3917.658 kb on - strand at 3917.746 kb on - strand, within IAI47_18405 at 3917.870 kb on - strand, within IAI47_18405 at 3917.870 kb on - strand, within IAI47_18405 at 3917.870 kb on - strand, within IAI47_18405 at 3917.870 kb on - strand, within IAI47_18405 at 3917.870 kb on - strand, within IAI47_18405 at 3917.884 kb on - strand, within IAI47_18405 at 3917.912 kb on - strand, within IAI47_18405 at 3917.983 kb on + strand, within IAI47_18405 at 3917.984 kb on - strand, within IAI47_18405 at 3917.984 kb on - strand, within IAI47_18405 at 3917.984 kb on - strand, within IAI47_18405 at 3917.984 kb on - strand, within IAI47_18405 at 3917.989 kb on + strand, within IAI47_18405 at 3917.989 kb on + strand, within IAI47_18405 at 3917.990 kb on - strand, within IAI47_18405 at 3918.056 kb on - strand, within IAI47_18405 at 3918.056 kb on - strand, within IAI47_18405 at 3918.056 kb on - strand, within IAI47_18405 at 3918.056 kb on - strand, within IAI47_18405 at 3918.125 kb on - strand, within IAI47_18405 at 3918.301 kb on - strand at 3918.301 kb on - strand at 3918.301 kb on - strand at 3918.301 kb on - strand at 3918.356 kb on - strand at 3918.365 kb on - strand at 3918.365 kb on - strand at 3918.365 kb on - strand at 3918.365 kb on - strand at 3918.373 kb on - strand at 3918.373 kb on - strand at 3918.384 kb on - strand at 3918.409 kb on - strand at 3918.409 kb on - strand at 3918.409 kb on - strand at 3918.409 kb on - strand at 3918.411 kb on - strand at 3918.411 kb on - strand at 3918.411 kb on - strand at 3918.442 kb on - strand at 3918.442 kb on - strand at 3918.442 kb on - strand at 3918.442 kb on - strand at 3918.442 kb on - strand at 3918.442 kb on - strand at 3918.442 kb on - strand at 3918.478 kb on - strand, within IAI47_18410 at 3918.478 kb on - strand, within IAI47_18410 at 3918.478 kb on - strand, within IAI47_18410 at 3918.567 kb on - strand, within IAI47_18410 at 3918.606 kb on + strand, within IAI47_18410 at 3918.607 kb on - strand, within IAI47_18410 at 3918.607 kb on - strand, within IAI47_18410 at 3918.718 kb on - strand, within IAI47_18410 at 3918.770 kb on + strand, within IAI47_18410 at 3918.771 kb on - strand, within IAI47_18410 at 3918.771 kb on - strand, within IAI47_18410 at 3918.771 kb on - strand, within IAI47_18410 at 3918.892 kb on - strand, within IAI47_18410 at 3918.892 kb on - strand, within IAI47_18410 at 3919.146 kb on + strand at 3919.147 kb on - strand at 3919.147 kb on - strand at 3919.147 kb on - strand at 3919.216 kb on - strand at 3919.216 kb on - strand at 3919.216 kb on - strand at 3919.226 kb on + strand at 3919.227 kb on - strand at 3919.227 kb on - strand at 3919.227 kb on - strand at 3919.227 kb on - strand at 3919.336 kb on - strand, within IAI47_18415 at 3919.336 kb on - strand, within IAI47_18415
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 3,916,671 + IAI47_18395 0.87 +0.5 3,916,671 + IAI47_18395 0.87 -0.4 3,916,688 + IAI47_18395 0.90 +3.6 3,916,688 + +2.0 3,916,688 + IAI47_18395 0.90 -0.1 3,916,689 - IAI47_18395 0.90 +0.4 3,916,692 + +0.9 3,916,692 + +1.2 3,916,692 + -0.8 3,916,692 + +1.8 3,916,693 - -0.7 3,916,693 - -1.1 3,916,693 - -0.3 3,916,697 + +0.1 3,916,697 + +0.4 3,916,698 - -0.5 3,916,725 + -0.4 3,916,725 + -0.2 3,916,726 - -2.1 3,916,726 - +1.4 3,916,726 - +0.6 3,916,726 - +0.8 3,916,732 + +0.2 3,916,732 + -1.1 3,916,733 - -1.2 3,916,733 - -1.5 3,916,733 - +1.1 3,916,752 - -0.1 3,916,752 - +3.9 3,916,838 - +1.0 3,916,872 + IAI47_18400 0.13 -1.8 3,917,002 - IAI47_18400 0.27 -1.3 3,917,002 - IAI47_18400 0.27 -2.0 3,917,234 - IAI47_18400 0.53 +0.6 3,917,234 - IAI47_18400 0.53 -1.4 3,917,234 - IAI47_18400 0.53 -1.3 3,917,345 + IAI47_18400 0.65 -1.7 3,917,346 - IAI47_18400 0.65 -1.0 3,917,358 + IAI47_18400 0.66 +0.6 3,917,575 + +2.6 3,917,658 - -2.7 3,917,658 - +0.3 3,917,746 - IAI47_18405 0.12 -1.2 3,917,870 - IAI47_18405 0.30 -0.1 3,917,870 - IAI47_18405 0.30 +0.4 3,917,870 - IAI47_18405 0.30 +0.5 3,917,870 - IAI47_18405 0.30 +0.7 3,917,870 - IAI47_18405 0.30 +2.2 3,917,884 - IAI47_18405 0.32 -0.1 3,917,912 - IAI47_18405 0.36 +0.4 3,917,983 + IAI47_18405 0.46 -1.6 3,917,984 - IAI47_18405 0.46 -0.9 3,917,984 - IAI47_18405 0.46 +0.0 3,917,984 - IAI47_18405 0.46 +0.1 3,917,984 - IAI47_18405 0.46 +0.2 3,917,989 + IAI47_18405 0.47 -1.4 3,917,989 + IAI47_18405 0.47 -1.8 3,917,990 - IAI47_18405 0.47 -2.2 3,918,056 - IAI47_18405 0.56 -2.7 3,918,056 - IAI47_18405 0.56 -0.8 3,918,056 - IAI47_18405 0.56 -1.9 3,918,056 - IAI47_18405 0.56 +0.7 3,918,125 - IAI47_18405 0.66 -1.2 3,918,301 - -0.1 3,918,301 - -1.9 3,918,301 - +0.2 3,918,301 - +0.3 3,918,356 - +0.3 3,918,365 - -0.8 3,918,365 - +0.4 3,918,365 - +0.9 3,918,365 - +0.6 3,918,373 - -1.4 3,918,373 - -0.6 3,918,384 - -0.7 3,918,409 - +1.2 3,918,409 - -0.8 3,918,409 - +0.1 3,918,409 - -1.4 3,918,411 - -0.1 3,918,411 - -2.3 3,918,411 - +0.8 3,918,442 - -1.7 3,918,442 - -0.5 3,918,442 - -0.4 3,918,442 - +0.6 3,918,442 - -1.8 3,918,442 - -0.0 3,918,442 - -2.7 3,918,478 - IAI47_18410 0.14 -0.5 3,918,478 - IAI47_18410 0.14 -0.4 3,918,478 - IAI47_18410 0.14 +1.5 3,918,567 - IAI47_18410 0.25 -1.4 3,918,606 + IAI47_18410 0.30 -2.2 3,918,607 - IAI47_18410 0.30 -0.1 3,918,607 - IAI47_18410 0.30 -0.7 3,918,718 - IAI47_18410 0.43 -0.9 3,918,770 + IAI47_18410 0.50 -2.4 3,918,771 - IAI47_18410 0.50 -3.0 3,918,771 - IAI47_18410 0.50 -1.7 3,918,771 - IAI47_18410 0.50 -1.1 3,918,892 - IAI47_18410 0.64 +0.5 3,918,892 - IAI47_18410 0.64 -1.8 3,919,146 + +1.0 3,919,147 - -0.3 3,919,147 - +1.6 3,919,147 - -1.0 3,919,216 - +1.6 3,919,216 - -1.8 3,919,216 - -0.8 3,919,226 + -1.5 3,919,227 - +0.6 3,919,227 - +0.5 3,919,227 - -2.5 3,919,227 - +0.3 3,919,336 - IAI47_18415 0.61 -1.9 3,919,336 - IAI47_18415 0.61 +0.4
Or see this region's nucleotide sequence