Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_04205 and IAI47_04210 overlap by 4 nucleotides IAI47_04210 and IAI47_04215 overlap by 4 nucleotides IAI47_04215 and IAI47_04220 are separated by 191 nucleotides IAI47_04220 and IAI47_04225 overlap by 7 nucleotides
IAI47_04205: IAI47_04205 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 883,159 to 883,875
_04205
IAI47_04210: IAI47_04210 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 883,872 to 884,357
_04210
IAI47_04215: IAI47_04215 - tRNA pseudouridine(13) synthase TruD, at 884,354 to 885,394
_04215
IAI47_04220: IAI47_04220 - 5'/3'-nucleotidase SurE, at 885,586 to 886,347
_04220
IAI47_04225: IAI47_04225 - protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 886,341 to 886,967
_04225
Position (kb)
884
885
886 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 883.859 kb on + strand at 883.859 kb on + strand at 883.859 kb on + strand at 884.366 kb on + strand at 884.366 kb on + strand at 884.366 kb on + strand at 884.366 kb on + strand at 884.366 kb on + strand at 884.366 kb on + strand at 884.489 kb on - strand, within IAI47_04215 at 884.530 kb on + strand, within IAI47_04215 at 884.530 kb on + strand, within IAI47_04215 at 884.531 kb on - strand, within IAI47_04215 at 884.531 kb on - strand, within IAI47_04215 at 884.531 kb on - strand, within IAI47_04215 at 884.534 kb on + strand, within IAI47_04215 at 884.534 kb on + strand, within IAI47_04215 at 884.534 kb on + strand, within IAI47_04215 at 884.534 kb on + strand, within IAI47_04215 at 884.534 kb on + strand, within IAI47_04215 at 884.534 kb on + strand, within IAI47_04215 at 884.534 kb on + strand, within IAI47_04215 at 884.535 kb on - strand, within IAI47_04215 at 884.535 kb on - strand, within IAI47_04215 at 884.535 kb on - strand, within IAI47_04215 at 884.535 kb on - strand, within IAI47_04215 at 884.535 kb on - strand, within IAI47_04215 at 884.542 kb on + strand, within IAI47_04215 at 884.543 kb on - strand, within IAI47_04215 at 884.564 kb on + strand, within IAI47_04215 at 884.564 kb on + strand, within IAI47_04215 at 884.565 kb on - strand, within IAI47_04215 at 884.565 kb on - strand, within IAI47_04215 at 884.653 kb on + strand, within IAI47_04215 at 884.698 kb on + strand, within IAI47_04215 at 884.698 kb on + strand, within IAI47_04215 at 884.698 kb on + strand, within IAI47_04215 at 884.698 kb on + strand, within IAI47_04215 at 884.698 kb on + strand, within IAI47_04215 at 884.698 kb on + strand, within IAI47_04215 at 884.699 kb on - strand, within IAI47_04215 at 884.699 kb on - strand, within IAI47_04215 at 884.816 kb on + strand, within IAI47_04215 at 884.816 kb on + strand, within IAI47_04215 at 884.816 kb on + strand, within IAI47_04215 at 884.816 kb on + strand, within IAI47_04215 at 884.816 kb on + strand, within IAI47_04215 at 884.816 kb on + strand, within IAI47_04215 at 884.817 kb on - strand, within IAI47_04215 at 884.817 kb on - strand, within IAI47_04215 at 884.817 kb on - strand, within IAI47_04215 at 884.851 kb on + strand, within IAI47_04215 at 884.852 kb on - strand, within IAI47_04215 at 884.950 kb on + strand, within IAI47_04215 at 884.950 kb on + strand, within IAI47_04215 at 884.951 kb on - strand, within IAI47_04215 at 884.951 kb on - strand, within IAI47_04215 at 885.191 kb on - strand, within IAI47_04215 at 885.191 kb on - strand, within IAI47_04215 at 885.266 kb on - strand, within IAI47_04215 at 885.392 kb on + strand at 885.392 kb on + strand at 885.392 kb on + strand at 885.393 kb on - strand at 885.393 kb on - strand at 885.393 kb on - strand at 885.410 kb on + strand at 885.411 kb on - strand at 885.425 kb on + strand at 885.425 kb on + strand at 885.425 kb on + strand at 885.459 kb on + strand at 885.459 kb on + strand at 885.459 kb on + strand at 885.459 kb on + strand at 885.459 kb on + strand at 885.460 kb on - strand at 885.460 kb on - strand at 885.460 kb on - strand at 885.460 kb on - strand at 885.460 kb on - strand at 885.460 kb on - strand at 885.465 kb on + strand at 885.466 kb on - strand at 885.466 kb on - strand at 885.488 kb on + strand at 885.488 kb on + strand at 885.488 kb on + strand at 885.489 kb on - strand at 885.489 kb on - strand at 885.491 kb on - strand at 885.534 kb on + strand at 885.534 kb on + strand at 885.593 kb on + strand at 885.631 kb on - strand at 885.711 kb on + strand, within IAI47_04220 at 885.711 kb on + strand, within IAI47_04220 at 885.711 kb on + strand, within IAI47_04220 at 885.742 kb on - strand, within IAI47_04220 at 885.747 kb on + strand, within IAI47_04220 at 885.748 kb on - strand, within IAI47_04220 at 885.802 kb on + strand, within IAI47_04220 at 885.802 kb on + strand, within IAI47_04220 at 885.803 kb on - strand, within IAI47_04220 at 885.859 kb on - strand, within IAI47_04220 at 885.859 kb on - strand, within IAI47_04220 at 885.892 kb on + strand, within IAI47_04220 at 885.892 kb on + strand, within IAI47_04220 at 885.892 kb on + strand, within IAI47_04220 at 885.892 kb on + strand, within IAI47_04220 at 885.893 kb on - strand, within IAI47_04220 at 885.901 kb on - strand, within IAI47_04220 at 885.976 kb on + strand, within IAI47_04220 at 886.056 kb on + strand, within IAI47_04220 at 886.167 kb on + strand, within IAI47_04220 at 886.168 kb on - strand, within IAI47_04220 at 886.168 kb on - strand, within IAI47_04220 at 886.175 kb on - strand, within IAI47_04220 at 886.175 kb on - strand, within IAI47_04220 at 886.175 kb on - strand, within IAI47_04220 at 886.181 kb on + strand, within IAI47_04220 at 886.182 kb on - strand, within IAI47_04220 at 886.182 kb on - strand, within IAI47_04220 at 886.182 kb on - strand, within IAI47_04220 at 886.246 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 883,859 + +1.4 883,859 + -1.9 883,859 + -0.6 884,366 + -0.1 884,366 + +3.3 884,366 + +0.4 884,366 + -0.3 884,366 + +1.0 884,366 + +2.0 884,489 - IAI47_04215 0.13 +0.4 884,530 + IAI47_04215 0.17 +1.9 884,530 + IAI47_04215 0.17 +2.2 884,531 - IAI47_04215 0.17 -0.3 884,531 - IAI47_04215 0.17 +0.4 884,531 - IAI47_04215 0.17 +1.1 884,534 + IAI47_04215 0.17 +0.8 884,534 + IAI47_04215 0.17 +1.0 884,534 + IAI47_04215 0.17 +1.8 884,534 + IAI47_04215 0.17 +2.8 884,534 + IAI47_04215 0.17 +0.3 884,534 + IAI47_04215 0.17 +0.7 884,534 + IAI47_04215 0.17 +1.2 884,535 - IAI47_04215 0.17 -0.0 884,535 - IAI47_04215 0.17 +1.8 884,535 - IAI47_04215 0.17 -1.2 884,535 - IAI47_04215 0.17 -0.5 884,535 - IAI47_04215 0.17 -0.8 884,542 + IAI47_04215 0.18 -1.9 884,543 - IAI47_04215 0.18 +0.3 884,564 + IAI47_04215 0.20 +1.3 884,564 + IAI47_04215 0.20 -1.4 884,565 - IAI47_04215 0.20 -0.9 884,565 - IAI47_04215 0.20 +0.0 884,653 + IAI47_04215 0.29 +0.1 884,698 + IAI47_04215 0.33 +0.1 884,698 + IAI47_04215 0.33 -2.5 884,698 + IAI47_04215 0.33 +1.2 884,698 + IAI47_04215 0.33 -0.3 884,698 + IAI47_04215 0.33 +2.2 884,698 + IAI47_04215 0.33 +1.0 884,699 - IAI47_04215 0.33 +0.6 884,699 - IAI47_04215 0.33 +0.4 884,816 + IAI47_04215 0.44 +2.1 884,816 + IAI47_04215 0.44 -1.4 884,816 + IAI47_04215 0.44 +0.9 884,816 + IAI47_04215 0.44 +3.1 884,816 + IAI47_04215 0.44 -0.4 884,816 + IAI47_04215 0.44 +1.5 884,817 - IAI47_04215 0.44 +0.8 884,817 - IAI47_04215 0.44 +2.1 884,817 - IAI47_04215 0.44 -2.1 884,851 + IAI47_04215 0.48 +1.0 884,852 - IAI47_04215 0.48 +1.0 884,950 + IAI47_04215 0.57 +0.1 884,950 + IAI47_04215 0.57 -0.0 884,951 - IAI47_04215 0.57 +0.2 884,951 - IAI47_04215 0.57 -1.8 885,191 - IAI47_04215 0.80 -1.9 885,191 - IAI47_04215 0.80 -0.4 885,266 - IAI47_04215 0.88 +0.7 885,392 + +2.5 885,392 + +2.2 885,392 + +0.1 885,393 - +1.1 885,393 - -0.7 885,393 - +2.2 885,410 + +0.1 885,411 - -0.4 885,425 + -0.7 885,425 + -0.2 885,425 + -0.9 885,459 + -1.1 885,459 + +0.6 885,459 + -0.6 885,459 + +1.0 885,459 + +1.0 885,460 - -1.5 885,460 - -0.1 885,460 - -0.7 885,460 - -0.8 885,460 - -0.2 885,460 - -2.9 885,465 + +3.2 885,466 - +0.3 885,466 - +0.9 885,488 + +1.6 885,488 + -3.3 885,488 + -0.6 885,489 - -0.7 885,489 - -0.4 885,491 - +0.4 885,534 + +0.4 885,534 + -1.8 885,593 + -1.1 885,631 - +0.4 885,711 + IAI47_04220 0.16 +1.8 885,711 + IAI47_04220 0.16 -1.2 885,711 + IAI47_04220 0.16 -0.8 885,742 - IAI47_04220 0.20 +2.2 885,747 + IAI47_04220 0.21 -1.5 885,748 - IAI47_04220 0.21 -0.3 885,802 + IAI47_04220 0.28 -0.4 885,802 + IAI47_04220 0.28 -0.8 885,803 - IAI47_04220 0.28 +0.8 885,859 - IAI47_04220 0.36 -2.7 885,859 - IAI47_04220 0.36 +0.1 885,892 + IAI47_04220 0.40 +1.3 885,892 + IAI47_04220 0.40 -0.6 885,892 + IAI47_04220 0.40 +1.0 885,892 + IAI47_04220 0.40 +0.7 885,893 - IAI47_04220 0.40 +0.2 885,901 - IAI47_04220 0.41 -0.9 885,976 + IAI47_04220 0.51 +0.3 886,056 + IAI47_04220 0.62 -0.1 886,167 + IAI47_04220 0.76 +0.1 886,168 - IAI47_04220 0.76 -0.4 886,168 - IAI47_04220 0.76 +0.5 886,175 - IAI47_04220 0.77 -1.4 886,175 - IAI47_04220 0.77 +1.3 886,175 - IAI47_04220 0.77 -2.2 886,181 + IAI47_04220 0.78 +1.5 886,182 - IAI47_04220 0.78 -1.5 886,182 - IAI47_04220 0.78 -0.5 886,182 - IAI47_04220 0.78 -1.4 886,246 + +1.1
Or see this region's nucleotide sequence