Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_03185 and IAI47_03190 are separated by 45 nucleotides IAI47_03190 and IAI47_03195 are separated by 141 nucleotides IAI47_03195 and IAI47_03200 are separated by 99 nucleotides
IAI47_03185: IAI47_03185 - DNA topoisomerase IV subunit A, at 658,990 to 661,263
_03185
IAI47_03190: IAI47_03190 - 1-acylglycerol-3-phosphate O-acyltransferase, at 661,309 to 662,046
_03190
IAI47_03195: IAI47_03195 - cell division protein FtsP, at 662,188 to 663,618
_03195
IAI47_03200: IAI47_03200 - 2,5-didehydrogluconate reductase DkgA, at 663,718 to 664,542
_03200
Position (kb) 662
663
664 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 661.261 kb on + strand at 661.266 kb on + strand at 662.171 kb on - strand at 662.187 kb on + strand at 662.253 kb on - strand at 662.464 kb on - strand, within IAI47_03195 at 662.556 kb on + strand, within IAI47_03195 at 662.852 kb on + strand, within IAI47_03195 at 662.939 kb on + strand, within IAI47_03195 at 662.939 kb on + strand, within IAI47_03195 at 663.050 kb on + strand, within IAI47_03195 at 663.054 kb on + strand, within IAI47_03195 at 663.147 kb on + strand, within IAI47_03195 at 663.148 kb on - strand, within IAI47_03195 at 663.154 kb on - strand, within IAI47_03195 at 663.185 kb on + strand, within IAI47_03195 at 663.551 kb on - strand at 663.616 kb on + strand at 663.616 kb on + strand at 663.616 kb on + strand at 663.616 kb on + strand at 663.657 kb on - strand at 663.657 kb on - strand at 663.660 kb on + strand at 663.660 kb on + strand at 663.660 kb on + strand at 663.661 kb on - strand at 663.661 kb on - strand at 663.661 kb on - strand at 663.667 kb on + strand at 663.668 kb on - strand at 663.668 kb on - strand at 663.668 kb on - strand at 663.668 kb on - strand at 663.668 kb on - strand at 663.692 kb on + strand at 663.692 kb on + strand at 663.692 kb on + strand at 663.692 kb on + strand at 663.693 kb on - strand at 663.719 kb on + strand at 663.719 kb on + strand at 663.719 kb on + strand at 663.719 kb on + strand at 663.719 kb on + strand at 663.720 kb on - strand at 663.720 kb on - strand at 663.720 kb on - strand at 663.720 kb on - strand at 663.720 kb on - strand at 663.723 kb on + strand at 663.723 kb on + strand at 663.723 kb on + strand at 663.723 kb on + strand at 663.723 kb on + strand at 663.724 kb on - strand at 663.724 kb on - strand at 663.774 kb on + strand at 663.774 kb on + strand at 663.775 kb on - strand at 663.792 kb on + strand at 663.793 kb on - strand at 663.816 kb on + strand, within IAI47_03200 at 663.816 kb on + strand, within IAI47_03200 at 663.816 kb on + strand, within IAI47_03200 at 663.817 kb on - strand, within IAI47_03200 at 663.817 kb on - strand, within IAI47_03200 at 663.817 kb on - strand, within IAI47_03200 at 663.918 kb on - strand, within IAI47_03200 at 663.918 kb on - strand, within IAI47_03200 at 663.918 kb on - strand, within IAI47_03200 at 663.918 kb on - strand, within IAI47_03200 at 663.918 kb on - strand, within IAI47_03200 at 663.936 kb on + strand, within IAI47_03200 at 663.936 kb on + strand, within IAI47_03200 at 663.936 kb on + strand, within IAI47_03200 at 663.936 kb on + strand, within IAI47_03200 at 663.936 kb on + strand, within IAI47_03200 at 663.937 kb on - strand, within IAI47_03200 at 663.937 kb on - strand, within IAI47_03200 at 663.937 kb on - strand, within IAI47_03200 at 663.937 kb on - strand, within IAI47_03200 at 664.032 kb on + strand, within IAI47_03200 at 664.032 kb on + strand, within IAI47_03200 at 664.033 kb on - strand, within IAI47_03200 at 664.069 kb on + strand, within IAI47_03200 at 664.070 kb on - strand, within IAI47_03200 at 664.128 kb on + strand, within IAI47_03200 at 664.128 kb on + strand, within IAI47_03200 at 664.129 kb on - strand, within IAI47_03200 at 664.129 kb on - strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.232 kb on + strand, within IAI47_03200 at 664.232 kb on + strand, within IAI47_03200 at 664.232 kb on + strand, within IAI47_03200 at 664.233 kb on - strand, within IAI47_03200 at 664.391 kb on + strand, within IAI47_03200 at 664.392 kb on - strand, within IAI47_03200 at 664.392 kb on - strand, within IAI47_03200 at 664.404 kb on + strand, within IAI47_03200 at 664.419 kb on - strand, within IAI47_03200 at 664.477 kb on + strand at 664.478 kb on - strand at 664.478 kb on - strand at 664.503 kb on + strand at 664.503 kb on + strand at 664.503 kb on + strand at 664.503 kb on + strand at 664.503 kb on + strand at 664.504 kb on - strand at 664.504 kb on - strand at 664.504 kb on - strand at 664.504 kb on - strand at 664.504 kb on - strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.543 kb on - strand at 664.543 kb on - strand at 664.543 kb on - strand at 664.543 kb on - strand at 664.589 kb on + strand at 664.589 kb on + strand at 664.589 kb on + strand at 664.589 kb on + strand at 664.589 kb on + strand at 664.589 kb on + strand at 664.590 kb on - strand at 664.590 kb on - strand at 664.590 kb on - strand at 664.590 kb on - strand at 664.596 kb on - strand at 664.596 kb on - strand at 664.618 kb on + strand at 664.618 kb on + strand at 664.618 kb on + strand at 664.618 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 661,261 + -0.2 661,266 + -1.5 662,171 - -0.4 662,187 + -0.1 662,253 - -0.4 662,464 - IAI47_03195 0.19 -2.3 662,556 + IAI47_03195 0.26 +1.1 662,852 + IAI47_03195 0.46 -0.3 662,939 + IAI47_03195 0.52 -2.3 662,939 + IAI47_03195 0.52 +1.2 663,050 + IAI47_03195 0.60 -0.6 663,054 + IAI47_03195 0.61 -0.5 663,147 + IAI47_03195 0.67 +0.5 663,148 - IAI47_03195 0.67 +0.8 663,154 - IAI47_03195 0.68 -2.1 663,185 + IAI47_03195 0.70 -0.7 663,551 - +1.1 663,616 + -1.3 663,616 + +0.3 663,616 + +1.1 663,616 + -1.1 663,657 - +1.0 663,657 - +0.1 663,660 + -0.9 663,660 + +0.6 663,660 + -0.2 663,661 - +1.0 663,661 - +0.4 663,661 - +0.1 663,667 + +2.2 663,668 - +1.3 663,668 - -1.4 663,668 - +0.7 663,668 - -1.4 663,668 - +2.4 663,692 + +1.8 663,692 + +0.3 663,692 + -0.4 663,692 + +1.9 663,693 - -0.1 663,719 + -1.9 663,719 + -0.7 663,719 + -0.4 663,719 + -1.3 663,719 + -0.3 663,720 - +1.4 663,720 - -1.0 663,720 - +1.4 663,720 - -0.4 663,720 - -1.6 663,723 + -0.9 663,723 + +0.6 663,723 + -0.2 663,723 + +0.6 663,723 + +1.0 663,724 - +1.5 663,724 - -0.4 663,774 + +0.5 663,774 + -1.6 663,775 - +0.8 663,792 + +0.3 663,793 - +0.7 663,816 + IAI47_03200 0.12 -0.8 663,816 + IAI47_03200 0.12 -2.8 663,816 + IAI47_03200 0.12 -1.7 663,817 - IAI47_03200 0.12 -0.9 663,817 - IAI47_03200 0.12 -1.9 663,817 - IAI47_03200 0.12 -0.5 663,918 - IAI47_03200 0.24 +0.2 663,918 - IAI47_03200 0.24 +0.1 663,918 - IAI47_03200 0.24 +0.1 663,918 - IAI47_03200 0.24 -0.1 663,918 - IAI47_03200 0.24 -0.6 663,936 + IAI47_03200 0.26 +0.1 663,936 + IAI47_03200 0.26 +1.4 663,936 + IAI47_03200 0.26 -0.8 663,936 + IAI47_03200 0.26 -1.1 663,936 + IAI47_03200 0.26 -0.3 663,937 - IAI47_03200 0.27 +0.8 663,937 - IAI47_03200 0.27 -1.6 663,937 - IAI47_03200 0.27 +0.2 663,937 - IAI47_03200 0.27 +0.2 664,032 + IAI47_03200 0.38 +0.2 664,032 + IAI47_03200 0.38 +1.8 664,033 - IAI47_03200 0.38 +0.1 664,069 + IAI47_03200 0.43 -0.3 664,070 - IAI47_03200 0.43 -0.5 664,128 + IAI47_03200 0.50 -1.2 664,128 + IAI47_03200 0.50 -1.3 664,129 - IAI47_03200 0.50 +0.8 664,129 - IAI47_03200 0.50 -0.4 664,196 + IAI47_03200 0.58 -1.6 664,196 + IAI47_03200 0.58 -1.4 664,196 + IAI47_03200 0.58 +1.0 664,196 + IAI47_03200 0.58 +0.4 664,196 + IAI47_03200 0.58 -1.2 664,196 + IAI47_03200 0.58 -1.2 664,196 + IAI47_03200 0.58 +1.0 664,196 + IAI47_03200 0.58 -0.6 664,197 - IAI47_03200 0.58 +0.8 664,197 - IAI47_03200 0.58 -2.1 664,197 - IAI47_03200 0.58 -0.7 664,197 - IAI47_03200 0.58 -0.9 664,197 - IAI47_03200 0.58 +0.4 664,197 - IAI47_03200 0.58 +3.9 664,197 - IAI47_03200 0.58 +0.6 664,197 - IAI47_03200 0.58 +1.0 664,197 - IAI47_03200 0.58 -0.5 664,197 - IAI47_03200 0.58 +0.7 664,197 - IAI47_03200 0.58 -2.9 664,197 - IAI47_03200 0.58 -0.1 664,197 - IAI47_03200 0.58 -0.4 664,197 - IAI47_03200 0.58 +0.5 664,197 - IAI47_03200 0.58 +1.1 664,197 - IAI47_03200 0.58 +0.4 664,197 - IAI47_03200 0.58 -0.6 664,232 + IAI47_03200 0.62 +1.7 664,232 + IAI47_03200 0.62 +1.5 664,232 + IAI47_03200 0.62 -2.5 664,233 - IAI47_03200 0.62 -2.5 664,391 + IAI47_03200 0.82 +0.0 664,392 - IAI47_03200 0.82 -0.7 664,392 - IAI47_03200 0.82 +1.4 664,404 + IAI47_03200 0.83 +0.5 664,419 - IAI47_03200 0.85 +1.0 664,477 + -2.3 664,478 - -0.2 664,478 - -1.4 664,503 + -0.1 664,503 + -0.3 664,503 + +0.9 664,503 + +0.7 664,503 + +0.3 664,504 - +1.9 664,504 - +1.6 664,504 - -1.1 664,504 - -1.6 664,504 - -1.6 664,542 + -0.3 664,542 + +2.2 664,542 + -2.1 664,542 + +0.7 664,542 + -1.2 664,542 + -0.6 664,542 + -0.6 664,542 + +2.3 664,542 + -1.7 664,542 + -0.8 664,543 - +1.1 664,543 - +1.2 664,543 - -0.2 664,543 - +0.1 664,589 + +0.9 664,589 + -0.3 664,589 + +1.3 664,589 + -0.6 664,589 + +0.6 664,589 + +0.7 664,590 - -1.6 664,590 - -0.5 664,590 - +1.9 664,590 - -0.7 664,596 - -2.2 664,596 - +0.4 664,618 + +0.9 664,618 + +1.0 664,618 + -1.6 664,618 + +0.4
Or see this region's nucleotide sequence