Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_00435 and IAI47_00440 are separated by 551 nucleotides IAI47_00440 and IAI47_00445 are separated by 233 nucleotides
IAI47_00435: IAI47_00435 - YihA family ribosome biogenesis GTP-binding protein, at 97,138 to 97,773
_00435
IAI47_00440: IAI47_00440 - Der GTPase-activating protein YihI, at 98,325 to 98,849
_00440
IAI47_00445: IAI47_00445 - oxygen-independent coproporphyrinogen III oxidase, at 99,083 to 100,456
_00445
Position (kb) 98
99 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 97.797 kb on - strand at 97.797 kb on - strand at 97.815 kb on - strand at 97.816 kb on + strand at 97.816 kb on + strand at 97.817 kb on - strand at 97.817 kb on - strand at 97.817 kb on - strand at 97.817 kb on - strand at 97.854 kb on + strand at 97.854 kb on + strand at 97.867 kb on - strand at 97.881 kb on + strand at 97.882 kb on - strand at 97.882 kb on - strand at 97.895 kb on - strand at 97.915 kb on + strand at 97.916 kb on - strand at 97.942 kb on - strand at 97.949 kb on + strand at 97.950 kb on - strand at 97.950 kb on - strand at 97.975 kb on + strand at 97.979 kb on + strand at 97.980 kb on - strand at 97.980 kb on - strand at 97.980 kb on - strand at 97.980 kb on - strand at 97.980 kb on - strand at 97.980 kb on - strand at 97.980 kb on - strand at 97.980 kb on - strand at 98.017 kb on + strand at 98.018 kb on - strand at 98.018 kb on - strand at 98.022 kb on + strand at 98.023 kb on - strand at 98.023 kb on - strand at 98.023 kb on - strand at 98.023 kb on - strand at 98.023 kb on - strand at 98.070 kb on - strand at 98.080 kb on + strand at 98.081 kb on - strand at 98.081 kb on - strand at 98.081 kb on - strand at 98.081 kb on - strand at 98.113 kb on - strand at 98.175 kb on + strand at 98.175 kb on + strand at 98.175 kb on + strand at 98.175 kb on + strand at 98.175 kb on + strand at 98.175 kb on + strand at 98.176 kb on - strand at 98.176 kb on - strand at 98.176 kb on - strand at 98.176 kb on - strand at 98.176 kb on - strand at 98.176 kb on - strand at 98.193 kb on + strand at 98.194 kb on - strand at 98.194 kb on - strand at 98.194 kb on - strand at 98.211 kb on + strand at 98.211 kb on + strand at 98.211 kb on + strand at 98.211 kb on + strand at 98.211 kb on + strand at 98.211 kb on + strand at 98.212 kb on - strand at 98.212 kb on - strand at 98.212 kb on - strand at 98.212 kb on - strand at 98.219 kb on + strand at 98.226 kb on + strand at 98.226 kb on + strand at 98.226 kb on + strand at 98.227 kb on - strand at 98.227 kb on - strand at 98.274 kb on + strand at 98.292 kb on + strand at 98.292 kb on + strand at 98.292 kb on + strand at 98.292 kb on + strand at 98.292 kb on + strand at 98.293 kb on - strand at 98.293 kb on - strand at 98.294 kb on + strand at 98.295 kb on - strand at 98.295 kb on - strand at 98.321 kb on + strand at 98.321 kb on + strand at 98.321 kb on + strand at 98.321 kb on + strand at 98.322 kb on - strand at 98.322 kb on - strand at 98.322 kb on - strand at 98.322 kb on - strand at 98.324 kb on + strand at 98.324 kb on + strand at 98.324 kb on + strand at 98.324 kb on + strand at 98.324 kb on + strand at 98.324 kb on + strand at 98.324 kb on + strand at 98.324 kb on + strand at 98.324 kb on + strand at 98.324 kb on + strand at 98.325 kb on - strand at 98.325 kb on - strand at 98.325 kb on - strand at 98.325 kb on - strand at 98.325 kb on - strand at 98.325 kb on - strand at 98.325 kb on - strand at 98.325 kb on - strand at 98.325 kb on - strand at 98.325 kb on - strand at 98.325 kb on - strand at 98.392 kb on - strand, within IAI47_00440 at 98.394 kb on - strand, within IAI47_00440 at 98.394 kb on - strand, within IAI47_00440 at 98.394 kb on - strand, within IAI47_00440 at 98.426 kb on + strand, within IAI47_00440 at 98.426 kb on + strand, within IAI47_00440 at 98.426 kb on + strand, within IAI47_00440 at 98.427 kb on - strand, within IAI47_00440 at 98.463 kb on - strand, within IAI47_00440 at 98.463 kb on - strand, within IAI47_00440 at 98.463 kb on - strand, within IAI47_00440 at 98.501 kb on + strand, within IAI47_00440 at 98.501 kb on + strand, within IAI47_00440 at 98.502 kb on - strand, within IAI47_00440 at 98.502 kb on - strand, within IAI47_00440 at 98.502 kb on - strand, within IAI47_00440 at 98.502 kb on - strand, within IAI47_00440 at 98.504 kb on + strand, within IAI47_00440 at 98.504 kb on + strand, within IAI47_00440 at 98.504 kb on + strand, within IAI47_00440 at 98.505 kb on - strand, within IAI47_00440 at 98.505 kb on - strand, within IAI47_00440 at 98.531 kb on + strand, within IAI47_00440 at 98.576 kb on + strand, within IAI47_00440 at 98.776 kb on + strand, within IAI47_00440 at 98.776 kb on + strand, within IAI47_00440 at 98.777 kb on - strand, within IAI47_00440 at 98.777 kb on - strand, within IAI47_00440 at 98.777 kb on - strand, within IAI47_00440 at 98.777 kb on - strand, within IAI47_00440 at 98.777 kb on - strand, within IAI47_00440 at 98.821 kb on + strand at 98.822 kb on + strand at 98.822 kb on + strand at 98.822 kb on + strand at 98.822 kb on + strand at 98.822 kb on + strand at 98.822 kb on + strand at 98.822 kb on + strand at 98.822 kb on + strand at 98.822 kb on + strand at 98.822 kb on + strand at 98.822 kb on + strand at 98.822 kb on + strand at 98.822 kb on + strand at 98.822 kb on + strand at 98.823 kb on - strand at 98.823 kb on - strand at 98.823 kb on - strand at 98.823 kb on - strand at 98.823 kb on - strand at 98.823 kb on - strand at 98.823 kb on - strand at 98.823 kb on - strand at 98.823 kb on - strand at 98.823 kb on - strand at 98.823 kb on - strand at 98.823 kb on - strand at 98.823 kb on - strand at 98.823 kb on - strand at 98.826 kb on + strand at 98.826 kb on + strand at 98.826 kb on + strand at 98.826 kb on + strand at 98.827 kb on - strand at 98.827 kb on - strand at 98.847 kb on + strand at 98.848 kb on - strand at 98.848 kb on - strand at 98.889 kb on - strand at 98.889 kb on - strand at 98.889 kb on - strand at 98.895 kb on + strand at 98.895 kb on + strand at 98.896 kb on - strand at 98.896 kb on - strand at 98.946 kb on + strand at 98.946 kb on + strand at 98.946 kb on + strand at 98.946 kb on + strand at 98.946 kb on + strand at 98.946 kb on + strand at 98.946 kb on + strand at 98.946 kb on + strand at 98.947 kb on - strand at 98.947 kb on - strand at 98.947 kb on - strand at 98.947 kb on - strand at 98.947 kb on - strand at 98.947 kb on - strand at 98.966 kb on + strand at 98.966 kb on + strand at 98.966 kb on + strand at 98.966 kb on + strand at 98.967 kb on - strand at 98.967 kb on - strand at 98.967 kb on - strand at 98.971 kb on + strand at 98.972 kb on - strand at 99.001 kb on + strand at 99.001 kb on + strand at 99.001 kb on + strand at 99.002 kb on - strand at 99.002 kb on - strand at 99.002 kb on - strand at 99.002 kb on - strand at 99.117 kb on + strand at 99.118 kb on - strand at 99.128 kb on + strand at 99.129 kb on - strand at 99.129 kb on - strand at 99.134 kb on + strand at 99.135 kb on - strand at 99.149 kb on + strand at 99.149 kb on + strand at 99.149 kb on + strand at 99.150 kb on - strand at 99.150 kb on - strand at 99.151 kb on + strand at 99.151 kb on + strand at 99.151 kb on + strand at 99.151 kb on + strand at 99.151 kb on + strand at 99.151 kb on + strand at 99.152 kb on - strand at 99.152 kb on - strand at 99.152 kb on - strand at 99.152 kb on - strand at 99.152 kb on - strand at 99.152 kb on - strand at 99.152 kb on - strand at 99.152 kb on - strand at 99.152 kb on - strand at 99.224 kb on + strand, within IAI47_00445 at 99.225 kb on - strand, within IAI47_00445 at 99.248 kb on + strand, within IAI47_00445 at 99.248 kb on + strand, within IAI47_00445 at 99.248 kb on + strand, within IAI47_00445 at 99.248 kb on + strand, within IAI47_00445 at 99.248 kb on + strand, within IAI47_00445 at 99.248 kb on + strand, within IAI47_00445 at 99.249 kb on - strand, within IAI47_00445 at 99.249 kb on - strand, within IAI47_00445 at 99.249 kb on - strand at 99.256 kb on + strand, within IAI47_00445 at 99.257 kb on - strand, within IAI47_00445 at 99.257 kb on - strand, within IAI47_00445 at 99.281 kb on + strand, within IAI47_00445 at 99.282 kb on - strand, within IAI47_00445 at 99.298 kb on + strand, within IAI47_00445 at 99.298 kb on + strand, within IAI47_00445 at 99.298 kb on + strand, within IAI47_00445 at 99.298 kb on + strand, within IAI47_00445 at 99.299 kb on - strand, within IAI47_00445 at 99.299 kb on - strand, within IAI47_00445 at 99.299 kb on - strand, within IAI47_00445 at 99.299 kb on - strand, within IAI47_00445 at 99.335 kb on + strand, within IAI47_00445 at 99.336 kb on - strand, within IAI47_00445 at 99.440 kb on - strand, within IAI47_00445 at 99.442 kb on + strand, within IAI47_00445 at 99.468 kb on + strand, within IAI47_00445 at 99.469 kb on - strand, within IAI47_00445 at 99.608 kb on - strand, within IAI47_00445 at 99.608 kb on - strand, within IAI47_00445 at 99.716 kb on + strand, within IAI47_00445 at 99.716 kb on + strand, within IAI47_00445 at 99.717 kb on - strand, within IAI47_00445 at 99.717 kb on - strand, within IAI47_00445 at 99.723 kb on + strand, within IAI47_00445 at 99.794 kb on - strand, within IAI47_00445 at 99.794 kb on - strand, within IAI47_00445 at 99.794 kb on - strand, within IAI47_00445 at 99.798 kb on + strand, within IAI47_00445 at 99.798 kb on + strand, within IAI47_00445 at 99.798 kb on + strand, within IAI47_00445 at 99.798 kb on + strand, within IAI47_00445 at 99.798 kb on + strand, within IAI47_00445 at 99.799 kb on - strand, within IAI47_00445 at 99.799 kb on - strand, within IAI47_00445 at 99.799 kb on - strand, within IAI47_00445 at 99.799 kb on - strand, within IAI47_00445 at 99.802 kb on + strand, within IAI47_00445 at 99.802 kb on + strand, within IAI47_00445 at 99.802 kb on + strand, within IAI47_00445 at 99.802 kb on + strand, within IAI47_00445 at 99.803 kb on - strand, within IAI47_00445 at 99.803 kb on - strand, within IAI47_00445 at 99.803 kb on - strand, within IAI47_00445 at 99.803 kb on - strand, within IAI47_00445 at 99.803 kb on - strand, within IAI47_00445 at 99.803 kb on - strand, within IAI47_00445 at 99.803 kb on - strand, within IAI47_00445 at 99.806 kb on + strand, within IAI47_00445 at 99.807 kb on - strand, within IAI47_00445 at 99.807 kb on - strand, within IAI47_00445 at 99.807 kb on - strand, within IAI47_00445
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 97,797 - +0.2 97,797 - +2.7 97,815 - -1.9 97,816 + -0.2 97,816 + -0.4 97,817 - +1.9 97,817 - -2.7 97,817 - +0.6 97,817 - +1.5 97,854 + +0.9 97,854 + -1.4 97,867 - +1.3 97,881 + -1.0 97,882 - -0.5 97,882 - +1.5 97,895 - +0.5 97,915 + -0.1 97,916 - -1.3 97,942 - -0.7 97,949 + +1.1 97,950 - -1.6 97,950 - +0.4 97,975 + +0.6 97,979 + +0.0 97,980 - +1.9 97,980 - -0.7 97,980 - -2.7 97,980 - -0.1 97,980 - +0.6 97,980 - +1.0 97,980 - -2.8 97,980 - +1.0 98,017 + -1.8 98,018 - +0.2 98,018 - +1.0 98,022 + -0.1 98,023 - +0.7 98,023 - +0.3 98,023 - -1.2 98,023 - +1.4 98,023 - -0.2 98,070 - +0.6 98,080 + -1.0 98,081 - +0.4 98,081 - +0.5 98,081 - +0.6 98,081 - +0.6 98,113 - +0.7 98,175 + +0.4 98,175 + +0.2 98,175 + +0.2 98,175 + +0.2 98,175 + +0.6 98,175 + -0.2 98,176 - -0.5 98,176 - -0.8 98,176 - +2.3 98,176 - +0.7 98,176 - -0.2 98,176 - -1.0 98,193 + -1.8 98,194 - -1.3 98,194 - +1.9 98,194 - +0.2 98,211 + -0.9 98,211 + +2.0 98,211 + -0.6 98,211 + +0.3 98,211 + -3.0 98,211 + -1.9 98,212 - +1.3 98,212 - -1.0 98,212 - +0.3 98,212 - -0.5 98,219 + -3.0 98,226 + +0.5 98,226 + +0.9 98,226 + +1.4 98,227 - +0.2 98,227 - +2.3 98,274 + +0.2 98,292 + +0.2 98,292 + +0.9 98,292 + -0.1 98,292 + -1.3 98,292 + +1.1 98,293 - +1.7 98,293 - -1.2 98,294 + +1.1 98,295 - +0.9 98,295 - -0.3 98,321 + +1.3 98,321 + -0.7 98,321 + -1.6 98,321 + -0.2 98,322 - -0.5 98,322 - +1.2 98,322 - -0.1 98,322 - -0.6 98,324 + +1.2 98,324 + -0.6 98,324 + -0.2 98,324 + -0.8 98,324 + +0.6 98,324 + -0.0 98,324 + +1.7 98,324 + -0.3 98,324 + -0.8 98,324 + -0.7 98,325 - -0.8 98,325 - +0.8 98,325 - -1.9 98,325 - -0.4 98,325 - +1.5 98,325 - +0.6 98,325 - -0.5 98,325 - +0.3 98,325 - -0.0 98,325 - +1.6 98,325 - +0.6 98,392 - IAI47_00440 0.13 +0.3 98,394 - IAI47_00440 0.13 -0.3 98,394 - IAI47_00440 0.13 +1.8 98,394 - IAI47_00440 0.13 +0.9 98,426 + IAI47_00440 0.19 -0.9 98,426 + IAI47_00440 0.19 +1.8 98,426 + IAI47_00440 0.19 +1.1 98,427 - IAI47_00440 0.19 -0.6 98,463 - IAI47_00440 0.26 -1.2 98,463 - IAI47_00440 0.26 +2.6 98,463 - IAI47_00440 0.26 +1.1 98,501 + IAI47_00440 0.34 +0.4 98,501 + IAI47_00440 0.34 +0.5 98,502 - IAI47_00440 0.34 +2.2 98,502 - IAI47_00440 0.34 -0.6 98,502 - IAI47_00440 0.34 +1.2 98,502 - IAI47_00440 0.34 -0.1 98,504 + IAI47_00440 0.34 -0.2 98,504 + IAI47_00440 0.34 -1.5 98,504 + IAI47_00440 0.34 +0.2 98,505 - IAI47_00440 0.34 +1.6 98,505 - IAI47_00440 0.34 +2.0 98,531 + IAI47_00440 0.39 +1.0 98,576 + IAI47_00440 0.48 +1.2 98,776 + IAI47_00440 0.86 +3.1 98,776 + IAI47_00440 0.86 -2.0 98,777 - IAI47_00440 0.86 +0.8 98,777 - IAI47_00440 0.86 +3.2 98,777 - IAI47_00440 0.86 +0.8 98,777 - IAI47_00440 0.86 +0.9 98,777 - IAI47_00440 0.86 +0.4 98,821 + -0.8 98,822 + +1.4 98,822 + +0.5 98,822 + -0.1 98,822 + +0.4 98,822 + +0.7 98,822 + +0.1 98,822 + +0.8 98,822 + +1.8 98,822 + -1.4 98,822 + -0.4 98,822 + +0.2 98,822 + -0.4 98,822 + +0.9 98,822 + +0.5 98,823 - -1.7 98,823 - -0.8 98,823 - -1.5 98,823 - +0.7 98,823 - -0.4 98,823 - +0.6 98,823 - +0.5 98,823 - -1.2 98,823 - +0.5 98,823 - -1.4 98,823 - +0.7 98,823 - +0.7 98,823 - -0.0 98,823 - +2.2 98,826 + -1.2 98,826 + -2.9 98,826 + +0.9 98,826 + -0.3 98,827 - +0.5 98,827 - -0.1 98,847 + +0.5 98,848 - -0.1 98,848 - +0.4 98,889 - -1.9 98,889 - -1.1 98,889 - -0.5 98,895 + +1.3 98,895 + +1.5 98,896 - +0.7 98,896 - -1.4 98,946 + +0.1 98,946 + -0.9 98,946 + +2.3 98,946 + +0.4 98,946 + +1.0 98,946 + -0.8 98,946 + -0.3 98,946 + -0.1 98,947 - -1.0 98,947 - +2.2 98,947 - +0.1 98,947 - -1.0 98,947 - -2.2 98,947 - +0.6 98,966 + -0.8 98,966 + -1.9 98,966 + -0.7 98,966 + -0.2 98,967 - +0.7 98,967 - -1.9 98,967 - -1.4 98,971 + -1.9 98,972 - -1.5 99,001 + -1.8 99,001 + -0.6 99,001 + +0.3 99,002 - -0.2 99,002 - -1.5 99,002 - -0.9 99,002 - -2.7 99,117 + -3.4 99,118 - -0.7 99,128 + -3.1 99,129 - -1.9 99,129 - -1.3 99,134 + -0.5 99,135 - -0.6 99,149 + -2.2 99,149 + +0.2 99,149 + -1.6 99,150 - -2.8 99,150 - -1.8 99,151 + -2.1 99,151 + -0.1 99,151 + -2.6 99,151 + -2.8 99,151 + +1.7 99,151 + -1.0 99,152 - -0.5 99,152 - -3.3 99,152 - -2.5 99,152 - -0.6 99,152 - -0.8 99,152 - -2.4 99,152 - -0.8 99,152 - -1.6 99,152 - -1.7 99,224 + IAI47_00445 0.10 +0.4 99,225 - IAI47_00445 0.10 -1.9 99,248 + IAI47_00445 0.12 -2.0 99,248 + IAI47_00445 0.12 -3.1 99,248 + IAI47_00445 0.12 -1.4 99,248 + IAI47_00445 0.12 -1.3 99,248 + IAI47_00445 0.12 -1.1 99,248 + IAI47_00445 0.12 -2.3 99,249 - IAI47_00445 0.12 -3.2 99,249 - IAI47_00445 0.12 +0.9 99,249 - -1.2 99,256 + IAI47_00445 0.13 +0.4 99,257 - IAI47_00445 0.13 -2.0 99,257 - IAI47_00445 0.13 -1.9 99,281 + IAI47_00445 0.14 -1.5 99,282 - IAI47_00445 0.14 +0.9 99,298 + IAI47_00445 0.16 -0.1 99,298 + IAI47_00445 0.16 -2.2 99,298 + IAI47_00445 0.16 -0.6 99,298 + IAI47_00445 0.16 -1.3 99,299 - IAI47_00445 0.16 -2.4 99,299 - IAI47_00445 0.16 -0.7 99,299 - IAI47_00445 0.16 -2.1 99,299 - IAI47_00445 0.16 -0.5 99,335 + IAI47_00445 0.18 -2.7 99,336 - IAI47_00445 0.18 -1.8 99,440 - IAI47_00445 0.26 -0.8 99,442 + IAI47_00445 0.26 -4.2 99,468 + IAI47_00445 0.28 -2.6 99,469 - IAI47_00445 0.28 -0.6 99,608 - IAI47_00445 0.38 -1.8 99,608 - IAI47_00445 0.38 -2.1 99,716 + IAI47_00445 0.46 -2.3 99,716 + IAI47_00445 0.46 -1.2 99,717 - IAI47_00445 0.46 -2.2 99,717 - IAI47_00445 0.46 -2.0 99,723 + IAI47_00445 0.47 +0.9 99,794 - IAI47_00445 0.52 -2.8 99,794 - IAI47_00445 0.52 +0.4 99,794 - IAI47_00445 0.52 -0.3 99,798 + IAI47_00445 0.52 -0.2 99,798 + IAI47_00445 0.52 -0.2 99,798 + IAI47_00445 0.52 -2.6 99,798 + IAI47_00445 0.52 -3.1 99,798 + IAI47_00445 0.52 -2.8 99,799 - IAI47_00445 0.52 -1.2 99,799 - IAI47_00445 0.52 -2.9 99,799 - IAI47_00445 0.52 -0.6 99,799 - IAI47_00445 0.52 -0.4 99,802 + IAI47_00445 0.52 +2.6 99,802 + IAI47_00445 0.52 -2.6 99,802 + IAI47_00445 0.52 -1.4 99,802 + IAI47_00445 0.52 -0.1 99,803 - IAI47_00445 0.52 -3.2 99,803 - IAI47_00445 0.52 -2.6 99,803 - IAI47_00445 0.52 -1.3 99,803 - IAI47_00445 0.52 -0.5 99,803 - IAI47_00445 0.52 -1.5 99,803 - IAI47_00445 0.52 -2.5 99,803 - IAI47_00445 0.52 -1.5 99,806 + IAI47_00445 0.53 -0.4 99,807 - IAI47_00445 0.53 -2.5 99,807 - IAI47_00445 0.53 -1.4 99,807 - IAI47_00445 0.53 +0.7
Or see this region's nucleotide sequence