Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_00115 and IAI47_00120 are separated by 32 nucleotides IAI47_00120 and IAI47_00125 are separated by 9 nucleotides IAI47_00125 and IAI47_00130 are separated by 139 nucleotides
IAI47_00115: IAI47_00115 - MFS transporter, at 24,510 to 25,790
_00115
IAI47_00120: IAI47_00120 - sugar kinase, at 25,823 to 26,782
_00120
IAI47_00125: IAI47_00125 - sugar phosphate isomerase/epimerase, at 26,792 to 27,541
_00125
IAI47_00130: IAI47_00130 - LacI family DNA-binding transcriptional regulator, at 27,681 to 28,706
_00130
Position (kb) 25
26
27 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 24.830 kb on + strand, within IAI47_00115 at 24.830 kb on + strand, within IAI47_00115 at 24.830 kb on + strand, within IAI47_00115 at 24.831 kb on - strand, within IAI47_00115 at 24.831 kb on - strand, within IAI47_00115 at 24.831 kb on - strand, within IAI47_00115 at 24.831 kb on - strand, within IAI47_00115 at 24.844 kb on + strand, within IAI47_00115 at 24.845 kb on - strand, within IAI47_00115 at 24.845 kb on - strand, within IAI47_00115 at 24.845 kb on - strand, within IAI47_00115 at 24.845 kb on - strand, within IAI47_00115 at 24.845 kb on - strand, within IAI47_00115 at 24.961 kb on + strand, within IAI47_00115 at 24.961 kb on + strand, within IAI47_00115 at 24.961 kb on + strand, within IAI47_00115 at 25.048 kb on + strand, within IAI47_00115 at 25.048 kb on + strand, within IAI47_00115 at 25.048 kb on + strand, within IAI47_00115 at 25.049 kb on - strand, within IAI47_00115 at 25.072 kb on + strand, within IAI47_00115 at 25.072 kb on + strand, within IAI47_00115 at 25.073 kb on - strand, within IAI47_00115 at 25.120 kb on + strand, within IAI47_00115 at 25.120 kb on + strand, within IAI47_00115 at 25.121 kb on - strand, within IAI47_00115 at 25.121 kb on - strand, within IAI47_00115 at 25.121 kb on - strand, within IAI47_00115 at 25.121 kb on - strand, within IAI47_00115 at 25.121 kb on - strand, within IAI47_00115 at 25.121 kb on - strand, within IAI47_00115 at 25.277 kb on + strand, within IAI47_00115 at 25.277 kb on + strand, within IAI47_00115 at 25.278 kb on - strand, within IAI47_00115 at 25.278 kb on - strand, within IAI47_00115 at 25.278 kb on - strand, within IAI47_00115 at 25.278 kb on - strand, within IAI47_00115 at 25.278 kb on - strand, within IAI47_00115 at 25.312 kb on + strand, within IAI47_00115 at 25.312 kb on + strand, within IAI47_00115 at 25.313 kb on - strand, within IAI47_00115 at 25.357 kb on - strand, within IAI47_00115 at 25.398 kb on - strand, within IAI47_00115 at 25.412 kb on + strand, within IAI47_00115 at 25.413 kb on - strand, within IAI47_00115 at 25.413 kb on - strand, within IAI47_00115 at 25.414 kb on + strand, within IAI47_00115 at 25.414 kb on + strand, within IAI47_00115 at 25.414 kb on + strand, within IAI47_00115 at 25.414 kb on + strand, within IAI47_00115 at 25.414 kb on + strand, within IAI47_00115 at 25.414 kb on + strand, within IAI47_00115 at 25.414 kb on + strand, within IAI47_00115 at 25.415 kb on - strand, within IAI47_00115 at 25.415 kb on - strand, within IAI47_00115 at 25.415 kb on - strand, within IAI47_00115 at 25.576 kb on + strand, within IAI47_00115 at 25.576 kb on + strand, within IAI47_00115 at 25.606 kb on + strand, within IAI47_00115 at 25.606 kb on + strand, within IAI47_00115 at 25.606 kb on + strand, within IAI47_00115 at 25.607 kb on - strand, within IAI47_00115 at 25.607 kb on - strand, within IAI47_00115 at 25.607 kb on - strand, within IAI47_00115 at 25.607 kb on - strand, within IAI47_00115 at 25.670 kb on + strand at 25.670 kb on + strand at 25.671 kb on - strand at 25.671 kb on - strand at 25.671 kb on - strand at 25.671 kb on - strand at 25.671 kb on - strand at 25.714 kb on + strand at 25.714 kb on + strand at 25.715 kb on - strand at 25.726 kb on + strand at 25.726 kb on + strand at 25.726 kb on + strand at 25.726 kb on + strand at 25.727 kb on - strand at 25.727 kb on - strand at 25.727 kb on - strand at 25.727 kb on - strand at 25.727 kb on - strand at 25.734 kb on - strand at 25.809 kb on + strand at 25.809 kb on + strand at 25.810 kb on - strand at 25.810 kb on - strand at 25.810 kb on - strand at 25.824 kb on + strand at 25.824 kb on + strand at 25.824 kb on + strand at 25.824 kb on + strand at 25.825 kb on - strand at 25.955 kb on - strand, within IAI47_00120 at 25.991 kb on - strand, within IAI47_00120 at 26.012 kb on + strand, within IAI47_00120 at 26.050 kb on + strand, within IAI47_00120 at 26.050 kb on + strand, within IAI47_00120 at 26.053 kb on - strand, within IAI47_00120 at 26.053 kb on - strand, within IAI47_00120 at 26.078 kb on + strand, within IAI47_00120 at 26.109 kb on + strand, within IAI47_00120 at 26.110 kb on - strand, within IAI47_00120 at 26.191 kb on - strand, within IAI47_00120 at 26.215 kb on + strand, within IAI47_00120 at 26.215 kb on + strand, within IAI47_00120 at 26.215 kb on + strand, within IAI47_00120 at 26.216 kb on - strand, within IAI47_00120 at 26.216 kb on - strand, within IAI47_00120 at 26.216 kb on - strand, within IAI47_00120 at 26.216 kb on - strand, within IAI47_00120 at 26.216 kb on - strand, within IAI47_00120 at 26.257 kb on + strand, within IAI47_00120 at 26.272 kb on + strand, within IAI47_00120 at 26.272 kb on + strand, within IAI47_00120 at 26.273 kb on - strand, within IAI47_00120 at 26.273 kb on - strand, within IAI47_00120 at 26.279 kb on - strand, within IAI47_00120 at 26.385 kb on + strand, within IAI47_00120 at 26.385 kb on + strand, within IAI47_00120 at 26.386 kb on - strand, within IAI47_00120 at 26.435 kb on - strand, within IAI47_00120 at 26.503 kb on - strand, within IAI47_00120 at 26.510 kb on - strand, within IAI47_00120 at 26.563 kb on + strand, within IAI47_00120 at 26.590 kb on + strand, within IAI47_00120 at 26.669 kb on - strand, within IAI47_00120 at 26.713 kb on + strand at 26.770 kb on + strand at 26.770 kb on + strand at 26.770 kb on + strand at 26.770 kb on + strand at 26.770 kb on + strand at 26.771 kb on - strand at 26.771 kb on - strand at 26.771 kb on - strand at 26.771 kb on - strand at 26.782 kb on + strand at 26.782 kb on + strand at 26.782 kb on + strand at 26.782 kb on + strand at 26.782 kb on + strand at 26.782 kb on + strand at 26.782 kb on + strand at 26.782 kb on + strand at 26.782 kb on + strand at 26.782 kb on + strand at 26.783 kb on - strand at 26.783 kb on - strand at 26.783 kb on - strand at 26.783 kb on - strand at 26.783 kb on - strand at 26.793 kb on + strand at 26.793 kb on + strand at 26.793 kb on + strand at 26.794 kb on - strand at 26.794 kb on - strand at 26.794 kb on - strand at 26.794 kb on - strand at 26.801 kb on - strand at 26.817 kb on + strand at 26.817 kb on + strand at 26.817 kb on + strand at 26.817 kb on + strand at 26.818 kb on - strand at 26.818 kb on - strand at 26.818 kb on - strand at 26.818 kb on - strand at 26.867 kb on - strand, within IAI47_00125 at 26.950 kb on + strand, within IAI47_00125 at 26.950 kb on + strand, within IAI47_00125 at 26.951 kb on - strand, within IAI47_00125 at 26.951 kb on - strand, within IAI47_00125 at 26.951 kb on - strand, within IAI47_00125 at 26.951 kb on - strand, within IAI47_00125 at 26.985 kb on + strand, within IAI47_00125 at 26.985 kb on + strand, within IAI47_00125 at 26.985 kb on + strand, within IAI47_00125 at 26.985 kb on + strand, within IAI47_00125 at 26.985 kb on + strand, within IAI47_00125 at 26.986 kb on - strand, within IAI47_00125 at 26.986 kb on - strand, within IAI47_00125 at 26.986 kb on - strand, within IAI47_00125 at 26.986 kb on - strand, within IAI47_00125 at 26.986 kb on - strand, within IAI47_00125 at 27.049 kb on + strand, within IAI47_00125 at 27.049 kb on + strand, within IAI47_00125 at 27.049 kb on + strand, within IAI47_00125 at 27.050 kb on - strand, within IAI47_00125 at 27.050 kb on - strand, within IAI47_00125 at 27.050 kb on - strand, within IAI47_00125 at 27.055 kb on + strand, within IAI47_00125 at 27.055 kb on + strand, within IAI47_00125 at 27.217 kb on + strand, within IAI47_00125 at 27.218 kb on - strand, within IAI47_00125 at 27.276 kb on + strand, within IAI47_00125 at 27.277 kb on - strand, within IAI47_00125 at 27.277 kb on - strand, within IAI47_00125 at 27.336 kb on + strand, within IAI47_00125 at 27.336 kb on + strand, within IAI47_00125 at 27.336 kb on + strand, within IAI47_00125 at 27.337 kb on - strand, within IAI47_00125 at 27.337 kb on - strand, within IAI47_00125 at 27.337 kb on - strand, within IAI47_00125 at 27.391 kb on + strand, within IAI47_00125 at 27.391 kb on + strand, within IAI47_00125 at 27.392 kb on - strand, within IAI47_00125 at 27.455 kb on - strand, within IAI47_00125 at 27.507 kb on + strand at 27.507 kb on + strand at 27.508 kb on - strand at 27.547 kb on - strand at 27.547 kb on - strand at 27.547 kb on - strand at 27.630 kb on + strand at 27.657 kb on + strand at 27.657 kb on + strand at 27.658 kb on - strand at 27.669 kb on + strand at 27.669 kb on + strand at 27.670 kb on - strand at 27.670 kb on - strand at 27.670 kb on - strand at 27.764 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 24,830 + IAI47_00115 0.25 +0.1 24,830 + IAI47_00115 0.25 +0.4 24,830 + IAI47_00115 0.25 -0.3 24,831 - IAI47_00115 0.25 +2.7 24,831 - IAI47_00115 0.25 +1.3 24,831 - IAI47_00115 0.25 -0.1 24,831 - IAI47_00115 0.25 -0.2 24,844 + IAI47_00115 0.26 -0.5 24,845 - IAI47_00115 0.26 -0.6 24,845 - IAI47_00115 0.26 +0.0 24,845 - IAI47_00115 0.26 -1.0 24,845 - IAI47_00115 0.26 -0.1 24,845 - IAI47_00115 0.26 -0.8 24,961 + IAI47_00115 0.35 -1.5 24,961 + IAI47_00115 0.35 +1.4 24,961 + IAI47_00115 0.35 +0.1 25,048 + IAI47_00115 0.42 -0.2 25,048 + IAI47_00115 0.42 -1.7 25,048 + IAI47_00115 0.42 -2.3 25,049 - IAI47_00115 0.42 +1.6 25,072 + IAI47_00115 0.44 -1.9 25,072 + IAI47_00115 0.44 +0.2 25,073 - IAI47_00115 0.44 -0.9 25,120 + IAI47_00115 0.48 +0.2 25,120 + IAI47_00115 0.48 -1.3 25,121 - IAI47_00115 0.48 -1.5 25,121 - IAI47_00115 0.48 -1.5 25,121 - IAI47_00115 0.48 +0.6 25,121 - IAI47_00115 0.48 -0.7 25,121 - IAI47_00115 0.48 +0.8 25,121 - IAI47_00115 0.48 +0.1 25,277 + IAI47_00115 0.60 +0.8 25,277 + IAI47_00115 0.60 +0.3 25,278 - IAI47_00115 0.60 +0.5 25,278 - IAI47_00115 0.60 -2.0 25,278 - IAI47_00115 0.60 -1.8 25,278 - IAI47_00115 0.60 -0.1 25,278 - IAI47_00115 0.60 +0.0 25,312 + IAI47_00115 0.63 +0.1 25,312 + IAI47_00115 0.63 +0.0 25,313 - IAI47_00115 0.63 -0.2 25,357 - IAI47_00115 0.66 -0.3 25,398 - IAI47_00115 0.69 +2.4 25,412 + IAI47_00115 0.70 -0.1 25,413 - IAI47_00115 0.70 -0.8 25,413 - IAI47_00115 0.70 +0.7 25,414 + IAI47_00115 0.71 +0.3 25,414 + IAI47_00115 0.71 +1.0 25,414 + IAI47_00115 0.71 +1.4 25,414 + IAI47_00115 0.71 -0.8 25,414 + IAI47_00115 0.71 +0.1 25,414 + IAI47_00115 0.71 -0.3 25,414 + IAI47_00115 0.71 +2.4 25,415 - IAI47_00115 0.71 -0.2 25,415 - IAI47_00115 0.71 -0.1 25,415 - IAI47_00115 0.71 -0.1 25,576 + IAI47_00115 0.83 -0.5 25,576 + IAI47_00115 0.83 +0.8 25,606 + IAI47_00115 0.86 -0.7 25,606 + IAI47_00115 0.86 +0.0 25,606 + IAI47_00115 0.86 -0.8 25,607 - IAI47_00115 0.86 -1.9 25,607 - IAI47_00115 0.86 +0.4 25,607 - IAI47_00115 0.86 -0.1 25,607 - IAI47_00115 0.86 -0.4 25,670 + +0.9 25,670 + -0.0 25,671 - -0.1 25,671 - -1.3 25,671 - -2.7 25,671 - -4.2 25,671 - -0.6 25,714 + -0.7 25,714 + +0.9 25,715 - -3.4 25,726 + +0.2 25,726 + +0.1 25,726 + -0.2 25,726 + +1.2 25,727 - +1.2 25,727 - -2.7 25,727 - -1.6 25,727 - -1.6 25,727 - -1.7 25,734 - +1.0 25,809 + -1.0 25,809 + -1.6 25,810 - -0.4 25,810 - +0.2 25,810 - +1.7 25,824 + -0.5 25,824 + -1.2 25,824 + -1.2 25,824 + +1.4 25,825 - +0.8 25,955 - IAI47_00120 0.14 -0.9 25,991 - IAI47_00120 0.17 +0.3 26,012 + IAI47_00120 0.20 -0.1 26,050 + IAI47_00120 0.24 +1.5 26,050 + IAI47_00120 0.24 -1.9 26,053 - IAI47_00120 0.24 -1.0 26,053 - IAI47_00120 0.24 +0.5 26,078 + IAI47_00120 0.27 +1.4 26,109 + IAI47_00120 0.30 -0.3 26,110 - IAI47_00120 0.30 +0.9 26,191 - IAI47_00120 0.38 -0.0 26,215 + IAI47_00120 0.41 +2.1 26,215 + IAI47_00120 0.41 -0.7 26,215 + IAI47_00120 0.41 -0.0 26,216 - IAI47_00120 0.41 -1.3 26,216 - IAI47_00120 0.41 -1.9 26,216 - IAI47_00120 0.41 +2.6 26,216 - IAI47_00120 0.41 -2.8 26,216 - IAI47_00120 0.41 -0.8 26,257 + IAI47_00120 0.45 +0.9 26,272 + IAI47_00120 0.47 +0.8 26,272 + IAI47_00120 0.47 -1.3 26,273 - IAI47_00120 0.47 -0.1 26,273 - IAI47_00120 0.47 -1.4 26,279 - IAI47_00120 0.47 -0.1 26,385 + IAI47_00120 0.59 +1.7 26,385 + IAI47_00120 0.59 -1.3 26,386 - IAI47_00120 0.59 +1.9 26,435 - IAI47_00120 0.64 +0.7 26,503 - IAI47_00120 0.71 -2.6 26,510 - IAI47_00120 0.72 +1.6 26,563 + IAI47_00120 0.77 -2.0 26,590 + IAI47_00120 0.80 -0.9 26,669 - IAI47_00120 0.88 +0.7 26,713 + +0.1 26,770 + +1.0 26,770 + -1.6 26,770 + +0.3 26,770 + -1.2 26,770 + -0.4 26,771 - -0.1 26,771 - +0.7 26,771 - +1.8 26,771 - -1.8 26,782 + -1.2 26,782 + +1.3 26,782 + -0.6 26,782 + -0.4 26,782 + +0.2 26,782 + -1.1 26,782 + +0.5 26,782 + -0.5 26,782 + -0.8 26,782 + -1.2 26,783 - -2.6 26,783 - +1.0 26,783 - +0.4 26,783 - -0.8 26,783 - -0.3 26,793 + -0.3 26,793 + +0.5 26,793 + -1.7 26,794 - +0.4 26,794 - +0.0 26,794 - -0.7 26,794 - -1.0 26,801 - -0.2 26,817 + -1.5 26,817 + -2.1 26,817 + +2.4 26,817 + -0.5 26,818 - -0.5 26,818 - -0.7 26,818 - +0.5 26,818 - +1.4 26,867 - IAI47_00125 0.10 +0.4 26,950 + IAI47_00125 0.21 +1.7 26,950 + IAI47_00125 0.21 +0.1 26,951 - IAI47_00125 0.21 +3.3 26,951 - IAI47_00125 0.21 +1.4 26,951 - IAI47_00125 0.21 -1.0 26,951 - IAI47_00125 0.21 +0.9 26,985 + IAI47_00125 0.26 -2.6 26,985 + IAI47_00125 0.26 +1.8 26,985 + IAI47_00125 0.26 -0.2 26,985 + IAI47_00125 0.26 +0.6 26,985 + IAI47_00125 0.26 -3.3 26,986 - IAI47_00125 0.26 +0.8 26,986 - IAI47_00125 0.26 +1.4 26,986 - IAI47_00125 0.26 +0.3 26,986 - IAI47_00125 0.26 -0.4 26,986 - IAI47_00125 0.26 -0.9 27,049 + IAI47_00125 0.34 +0.4 27,049 + IAI47_00125 0.34 -2.2 27,049 + IAI47_00125 0.34 +0.4 27,050 - IAI47_00125 0.34 -1.2 27,050 - IAI47_00125 0.34 +4.3 27,050 - IAI47_00125 0.34 +0.5 27,055 + IAI47_00125 0.35 +0.4 27,055 + IAI47_00125 0.35 +0.3 27,217 + IAI47_00125 0.57 +0.9 27,218 - IAI47_00125 0.57 -1.5 27,276 + IAI47_00125 0.65 +0.8 27,277 - IAI47_00125 0.65 -0.9 27,277 - IAI47_00125 0.65 +0.7 27,336 + IAI47_00125 0.73 +2.5 27,336 + IAI47_00125 0.73 -0.1 27,336 + IAI47_00125 0.73 -2.3 27,337 - IAI47_00125 0.73 +1.0 27,337 - IAI47_00125 0.73 +0.1 27,337 - IAI47_00125 0.73 +1.2 27,391 + IAI47_00125 0.80 +3.9 27,391 + IAI47_00125 0.80 +0.6 27,392 - IAI47_00125 0.80 -1.3 27,455 - IAI47_00125 0.88 -1.6 27,507 + -1.0 27,507 + +0.5 27,508 - +0.3 27,547 - +0.8 27,547 - -1.9 27,547 - +3.8 27,630 + +0.0 27,657 + -1.4 27,657 + +0.4 27,658 - +2.4 27,669 + -0.6 27,669 + -0.9 27,670 - -1.1 27,670 - -1.1 27,670 - -1.2 27,764 + +1.1
Or see this region's nucleotide sequence