Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_11995 and IAI47_12000 are separated by 39 nucleotides IAI47_12000 and IAI47_12005 are separated by 16 nucleotides IAI47_12005 and IAI47_12010 are separated by 11 nucleotides
IAI47_11995: IAI47_11995 - beta-N-acetylhexosaminidase, at 2,541,486 to 2,542,526
_11995
IAI47_12000: IAI47_12000 - thiamine kinase, at 2,542,566 to 2,543,348
_12000
IAI47_12005: IAI47_12005 - penicillin-binding protein activator LpoB, at 2,543,365 to 2,543,961
_12005
IAI47_12010: IAI47_12010 - YcfL family protein, at 2,543,973 to 2,544,341
_12010
Position (kb)
2542
2543
2544 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2541.730 kb on + strand, within IAI47_11995 at 2541.731 kb on - strand, within IAI47_11995 at 2541.731 kb on - strand, within IAI47_11995 at 2541.731 kb on - strand, within IAI47_11995 at 2541.731 kb on - strand, within IAI47_11995 at 2541.731 kb on - strand, within IAI47_11995 at 2541.826 kb on + strand, within IAI47_11995 at 2541.865 kb on + strand, within IAI47_11995 at 2541.865 kb on + strand, within IAI47_11995 at 2541.866 kb on - strand, within IAI47_11995 at 2541.866 kb on - strand, within IAI47_11995 at 2541.866 kb on - strand, within IAI47_11995 at 2541.866 kb on - strand, within IAI47_11995 at 2541.984 kb on - strand, within IAI47_11995 at 2542.025 kb on + strand, within IAI47_11995 at 2542.026 kb on - strand, within IAI47_11995 at 2542.065 kb on - strand, within IAI47_11995 at 2542.136 kb on + strand, within IAI47_11995 at 2542.136 kb on + strand, within IAI47_11995 at 2542.136 kb on + strand, within IAI47_11995 at 2542.136 kb on + strand, within IAI47_11995 at 2542.286 kb on + strand, within IAI47_11995 at 2542.287 kb on - strand, within IAI47_11995 at 2542.480 kb on + strand at 2542.481 kb on - strand at 2542.527 kb on - strand at 2542.527 kb on - strand at 2542.546 kb on + strand at 2542.546 kb on + strand at 2542.547 kb on - strand at 2542.547 kb on - strand at 2542.559 kb on - strand at 2542.568 kb on - strand at 2542.568 kb on - strand at 2542.602 kb on + strand at 2542.603 kb on - strand at 2542.603 kb on - strand at 2542.603 kb on - strand at 2542.603 kb on - strand at 2542.603 kb on - strand at 2542.729 kb on + strand, within IAI47_12000 at 2542.729 kb on + strand, within IAI47_12000 at 2542.729 kb on + strand, within IAI47_12000 at 2542.729 kb on + strand, within IAI47_12000 at 2542.730 kb on - strand, within IAI47_12000 at 2542.730 kb on - strand, within IAI47_12000 at 2542.814 kb on - strand, within IAI47_12000 at 2542.851 kb on + strand, within IAI47_12000 at 2542.851 kb on + strand, within IAI47_12000 at 2542.851 kb on + strand, within IAI47_12000 at 2542.851 kb on + strand, within IAI47_12000 at 2542.851 kb on + strand, within IAI47_12000 at 2542.851 kb on + strand, within IAI47_12000 at 2542.852 kb on - strand, within IAI47_12000 at 2542.852 kb on - strand, within IAI47_12000 at 2542.852 kb on - strand, within IAI47_12000 at 2542.852 kb on - strand, within IAI47_12000 at 2542.852 kb on - strand, within IAI47_12000 at 2542.852 kb on - strand, within IAI47_12000 at 2542.852 kb on - strand, within IAI47_12000 at 2542.852 kb on - strand, within IAI47_12000 at 2542.852 kb on - strand, within IAI47_12000 at 2542.967 kb on + strand, within IAI47_12000 at 2542.967 kb on + strand, within IAI47_12000 at 2542.967 kb on + strand, within IAI47_12000 at 2542.967 kb on + strand, within IAI47_12000 at 2542.967 kb on + strand, within IAI47_12000 at 2542.968 kb on - strand, within IAI47_12000 at 2542.968 kb on - strand, within IAI47_12000 at 2542.978 kb on + strand, within IAI47_12000 at 2542.989 kb on + strand, within IAI47_12000 at 2542.990 kb on - strand, within IAI47_12000 at 2542.990 kb on - strand, within IAI47_12000 at 2543.121 kb on - strand, within IAI47_12000 at 2543.121 kb on - strand, within IAI47_12000 at 2543.142 kb on + strand, within IAI47_12000 at 2543.142 kb on + strand, within IAI47_12000 at 2543.220 kb on - strand, within IAI47_12000 at 2543.220 kb on - strand, within IAI47_12000 at 2543.247 kb on - strand, within IAI47_12000 at 2543.307 kb on - strand at 2543.307 kb on - strand at 2543.366 kb on + strand at 2543.366 kb on + strand at 2543.366 kb on + strand at 2543.367 kb on - strand at 2543.448 kb on + strand, within IAI47_12005 at 2543.449 kb on - strand, within IAI47_12005 at 2543.467 kb on - strand, within IAI47_12005 at 2543.471 kb on + strand, within IAI47_12005 at 2543.471 kb on + strand, within IAI47_12005 at 2543.472 kb on - strand, within IAI47_12005 at 2543.472 kb on - strand, within IAI47_12005 at 2543.472 kb on - strand, within IAI47_12005 at 2543.472 kb on - strand, within IAI47_12005 at 2543.472 kb on - strand, within IAI47_12005 at 2543.480 kb on + strand, within IAI47_12005 at 2543.480 kb on + strand, within IAI47_12005 at 2543.480 kb on + strand, within IAI47_12005 at 2543.480 kb on + strand, within IAI47_12005 at 2543.480 kb on + strand, within IAI47_12005 at 2543.480 kb on + strand, within IAI47_12005 at 2543.480 kb on + strand, within IAI47_12005 at 2543.481 kb on - strand, within IAI47_12005 at 2543.481 kb on - strand, within IAI47_12005 at 2543.481 kb on - strand, within IAI47_12005 at 2543.481 kb on - strand, within IAI47_12005 at 2543.481 kb on - strand, within IAI47_12005 at 2543.481 kb on - strand, within IAI47_12005 at 2543.481 kb on - strand, within IAI47_12005 at 2543.481 kb on - strand, within IAI47_12005 at 2543.481 kb on - strand, within IAI47_12005 at 2543.518 kb on - strand, within IAI47_12005 at 2543.604 kb on + strand, within IAI47_12005 at 2543.611 kb on - strand, within IAI47_12005 at 2543.613 kb on + strand, within IAI47_12005 at 2543.613 kb on + strand, within IAI47_12005 at 2543.613 kb on + strand, within IAI47_12005 at 2543.613 kb on + strand, within IAI47_12005 at 2543.613 kb on + strand, within IAI47_12005 at 2543.614 kb on - strand, within IAI47_12005 at 2543.728 kb on + strand, within IAI47_12005 at 2543.728 kb on + strand, within IAI47_12005 at 2543.729 kb on - strand, within IAI47_12005 at 2543.729 kb on - strand, within IAI47_12005 at 2543.772 kb on + strand, within IAI47_12005 at 2543.772 kb on + strand, within IAI47_12005 at 2543.772 kb on + strand, within IAI47_12005 at 2543.772 kb on + strand, within IAI47_12005 at 2543.772 kb on + strand, within IAI47_12005 at 2543.941 kb on - strand at 2543.947 kb on - strand at 2543.947 kb on - strand at 2543.956 kb on + strand at 2543.957 kb on - strand at 2543.974 kb on + strand at 2543.980 kb on + strand at 2543.980 kb on + strand at 2543.980 kb on + strand at 2543.980 kb on + strand at 2543.981 kb on - strand at 2543.981 kb on - strand at 2543.986 kb on + strand at 2543.986 kb on + strand at 2543.999 kb on + strand at 2543.999 kb on + strand at 2544.000 kb on - strand at 2544.036 kb on + strand, within IAI47_12010 at 2544.036 kb on + strand, within IAI47_12010 at 2544.037 kb on - strand, within IAI47_12010 at 2544.109 kb on + strand, within IAI47_12010 at 2544.110 kb on - strand, within IAI47_12010 at 2544.115 kb on + strand, within IAI47_12010 at 2544.115 kb on + strand, within IAI47_12010 at 2544.118 kb on + strand, within IAI47_12010 at 2544.119 kb on - strand, within IAI47_12010 at 2544.124 kb on + strand, within IAI47_12010 at 2544.124 kb on + strand, within IAI47_12010 at 2544.155 kb on + strand, within IAI47_12010 at 2544.155 kb on + strand, within IAI47_12010 at 2544.156 kb on - strand, within IAI47_12010 at 2544.156 kb on - strand, within IAI47_12010 at 2544.157 kb on + strand, within IAI47_12010 at 2544.157 kb on + strand, within IAI47_12010 at 2544.157 kb on + strand, within IAI47_12010 at 2544.157 kb on + strand, within IAI47_12010 at 2544.157 kb on + strand, within IAI47_12010 at 2544.158 kb on - strand, within IAI47_12010 at 2544.158 kb on - strand, within IAI47_12010 at 2544.158 kb on - strand, within IAI47_12010 at 2544.215 kb on + strand, within IAI47_12010 at 2544.216 kb on - strand, within IAI47_12010 at 2544.216 kb on - strand, within IAI47_12010 at 2544.218 kb on + strand, within IAI47_12010 at 2544.218 kb on + strand, within IAI47_12010 at 2544.219 kb on - strand, within IAI47_12010 at 2544.266 kb on + strand, within IAI47_12010 at 2544.266 kb on + strand, within IAI47_12010 at 2544.266 kb on + strand, within IAI47_12010 at 2544.266 kb on + strand, within IAI47_12010 at 2544.266 kb on - strand, within IAI47_12010 at 2544.267 kb on - strand, within IAI47_12010 at 2544.267 kb on - strand, within IAI47_12010 at 2544.267 kb on - strand, within IAI47_12010 at 2544.267 kb on - strand, within IAI47_12010 at 2544.267 kb on - strand, within IAI47_12010 at 2544.267 kb on - strand, within IAI47_12010 at 2544.282 kb on - strand, within IAI47_12010 at 2544.282 kb on - strand, within IAI47_12010 at 2544.341 kb on + strand at 2544.341 kb on + strand at 2544.341 kb on + strand at 2544.341 kb on + strand at 2544.342 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,541,730 + IAI47_11995 0.23 -0.6 2,541,731 - IAI47_11995 0.24 +0.1 2,541,731 - IAI47_11995 0.24 -1.0 2,541,731 - IAI47_11995 0.24 -0.1 2,541,731 - IAI47_11995 0.24 -2.2 2,541,731 - IAI47_11995 0.24 +2.0 2,541,826 + IAI47_11995 0.33 -0.7 2,541,865 + IAI47_11995 0.36 -0.6 2,541,865 + IAI47_11995 0.36 -0.8 2,541,866 - IAI47_11995 0.37 -0.1 2,541,866 - IAI47_11995 0.37 +0.0 2,541,866 - IAI47_11995 0.37 -1.3 2,541,866 - IAI47_11995 0.37 +1.0 2,541,984 - IAI47_11995 0.48 -0.9 2,542,025 + IAI47_11995 0.52 -0.0 2,542,026 - IAI47_11995 0.52 +0.5 2,542,065 - IAI47_11995 0.56 -0.1 2,542,136 + IAI47_11995 0.62 +0.5 2,542,136 + IAI47_11995 0.62 +0.0 2,542,136 + IAI47_11995 0.62 -2.6 2,542,136 + IAI47_11995 0.62 -2.5 2,542,286 + IAI47_11995 0.77 -0.1 2,542,287 - IAI47_11995 0.77 +0.9 2,542,480 + -0.4 2,542,481 - +0.4 2,542,527 - +0.3 2,542,527 - +0.3 2,542,546 + -1.3 2,542,546 + -0.7 2,542,547 - +1.0 2,542,547 - +0.6 2,542,559 - -0.1 2,542,568 - -0.0 2,542,568 - -0.6 2,542,602 + -0.2 2,542,603 - +1.2 2,542,603 - -0.7 2,542,603 - +0.1 2,542,603 - -0.7 2,542,603 - -0.4 2,542,729 + IAI47_12000 0.21 -0.0 2,542,729 + IAI47_12000 0.21 -0.4 2,542,729 + IAI47_12000 0.21 -0.7 2,542,729 + IAI47_12000 0.21 +1.5 2,542,730 - IAI47_12000 0.21 +2.6 2,542,730 - IAI47_12000 0.21 -0.0 2,542,814 - IAI47_12000 0.32 +0.1 2,542,851 + IAI47_12000 0.36 +0.9 2,542,851 + IAI47_12000 0.36 +0.4 2,542,851 + IAI47_12000 0.36 +1.9 2,542,851 + IAI47_12000 0.36 +1.6 2,542,851 + IAI47_12000 0.36 +1.0 2,542,851 + IAI47_12000 0.36 +0.4 2,542,852 - IAI47_12000 0.37 +1.5 2,542,852 - IAI47_12000 0.37 +0.8 2,542,852 - IAI47_12000 0.37 -0.0 2,542,852 - IAI47_12000 0.37 -0.4 2,542,852 - IAI47_12000 0.37 -0.2 2,542,852 - IAI47_12000 0.37 +1.4 2,542,852 - IAI47_12000 0.37 -1.4 2,542,852 - IAI47_12000 0.37 +0.9 2,542,852 - IAI47_12000 0.37 +0.1 2,542,967 + IAI47_12000 0.51 +0.6 2,542,967 + IAI47_12000 0.51 +0.6 2,542,967 + IAI47_12000 0.51 +1.1 2,542,967 + IAI47_12000 0.51 +1.0 2,542,967 + IAI47_12000 0.51 -0.2 2,542,968 - IAI47_12000 0.51 +2.6 2,542,968 - IAI47_12000 0.51 +1.7 2,542,978 + IAI47_12000 0.53 +0.3 2,542,989 + IAI47_12000 0.54 +1.5 2,542,990 - IAI47_12000 0.54 -0.8 2,542,990 - IAI47_12000 0.54 +0.3 2,543,121 - IAI47_12000 0.71 -0.8 2,543,121 - IAI47_12000 0.71 +1.3 2,543,142 + IAI47_12000 0.74 -0.2 2,543,142 + IAI47_12000 0.74 -0.2 2,543,220 - IAI47_12000 0.84 +0.3 2,543,220 - IAI47_12000 0.84 +1.4 2,543,247 - IAI47_12000 0.87 +1.5 2,543,307 - -0.1 2,543,307 - +2.6 2,543,366 + +0.3 2,543,366 + +0.7 2,543,366 + +0.2 2,543,367 - -0.5 2,543,448 + IAI47_12005 0.14 +0.7 2,543,449 - IAI47_12005 0.14 +0.3 2,543,467 - IAI47_12005 0.17 -0.1 2,543,471 + IAI47_12005 0.18 +0.3 2,543,471 + IAI47_12005 0.18 +0.0 2,543,472 - IAI47_12005 0.18 +0.2 2,543,472 - IAI47_12005 0.18 +0.1 2,543,472 - IAI47_12005 0.18 -2.4 2,543,472 - IAI47_12005 0.18 -0.2 2,543,472 - IAI47_12005 0.18 -0.8 2,543,480 + IAI47_12005 0.19 +1.4 2,543,480 + IAI47_12005 0.19 +0.9 2,543,480 + IAI47_12005 0.19 -0.2 2,543,480 + IAI47_12005 0.19 +1.7 2,543,480 + IAI47_12005 0.19 +0.7 2,543,480 + IAI47_12005 0.19 +1.9 2,543,480 + IAI47_12005 0.19 +1.1 2,543,481 - IAI47_12005 0.19 -0.9 2,543,481 - IAI47_12005 0.19 -1.4 2,543,481 - IAI47_12005 0.19 -0.9 2,543,481 - IAI47_12005 0.19 -1.4 2,543,481 - IAI47_12005 0.19 -0.6 2,543,481 - IAI47_12005 0.19 -0.4 2,543,481 - IAI47_12005 0.19 -0.5 2,543,481 - IAI47_12005 0.19 -0.0 2,543,481 - IAI47_12005 0.19 -1.5 2,543,518 - IAI47_12005 0.26 -0.7 2,543,604 + IAI47_12005 0.40 +1.3 2,543,611 - IAI47_12005 0.41 +0.4 2,543,613 + IAI47_12005 0.42 -0.4 2,543,613 + IAI47_12005 0.42 +1.3 2,543,613 + IAI47_12005 0.42 +0.1 2,543,613 + IAI47_12005 0.42 +1.9 2,543,613 + IAI47_12005 0.42 +1.4 2,543,614 - IAI47_12005 0.42 -0.2 2,543,728 + IAI47_12005 0.61 -1.7 2,543,728 + IAI47_12005 0.61 -1.2 2,543,729 - IAI47_12005 0.61 +0.7 2,543,729 - IAI47_12005 0.61 +1.4 2,543,772 + IAI47_12005 0.68 -0.7 2,543,772 + IAI47_12005 0.68 -0.6 2,543,772 + IAI47_12005 0.68 +2.0 2,543,772 + IAI47_12005 0.68 +0.2 2,543,772 + IAI47_12005 0.68 +0.6 2,543,941 - +1.4 2,543,947 - +0.9 2,543,947 - -0.9 2,543,956 + +0.2 2,543,957 - -2.0 2,543,974 + +1.1 2,543,980 + -0.3 2,543,980 + +0.3 2,543,980 + +2.6 2,543,980 + +0.1 2,543,981 - -2.1 2,543,981 - +0.4 2,543,986 + -0.1 2,543,986 + +1.0 2,543,999 + +0.8 2,543,999 + +0.7 2,544,000 - -1.3 2,544,036 + IAI47_12010 0.17 +0.7 2,544,036 + IAI47_12010 0.17 +1.6 2,544,037 - IAI47_12010 0.17 -0.7 2,544,109 + IAI47_12010 0.37 +0.1 2,544,110 - IAI47_12010 0.37 -0.3 2,544,115 + IAI47_12010 0.38 -0.9 2,544,115 + IAI47_12010 0.38 +0.2 2,544,118 + IAI47_12010 0.39 -2.4 2,544,119 - IAI47_12010 0.40 +1.4 2,544,124 + IAI47_12010 0.41 +1.8 2,544,124 + IAI47_12010 0.41 +0.3 2,544,155 + IAI47_12010 0.49 +2.8 2,544,155 + IAI47_12010 0.49 +1.8 2,544,156 - IAI47_12010 0.50 +0.2 2,544,156 - IAI47_12010 0.50 +0.3 2,544,157 + IAI47_12010 0.50 -1.3 2,544,157 + IAI47_12010 0.50 -0.8 2,544,157 + IAI47_12010 0.50 +0.0 2,544,157 + IAI47_12010 0.50 -0.9 2,544,157 + IAI47_12010 0.50 +0.1 2,544,158 - IAI47_12010 0.50 +1.2 2,544,158 - IAI47_12010 0.50 -1.0 2,544,158 - IAI47_12010 0.50 +1.6 2,544,215 + IAI47_12010 0.66 +1.3 2,544,216 - IAI47_12010 0.66 +1.0 2,544,216 - IAI47_12010 0.66 +1.8 2,544,218 + IAI47_12010 0.66 +2.0 2,544,218 + IAI47_12010 0.66 -0.5 2,544,219 - IAI47_12010 0.67 -0.5 2,544,266 + IAI47_12010 0.79 +0.9 2,544,266 + IAI47_12010 0.79 +0.7 2,544,266 + IAI47_12010 0.79 -0.2 2,544,266 + IAI47_12010 0.79 -1.0 2,544,266 - IAI47_12010 0.79 +0.1 2,544,267 - IAI47_12010 0.80 -1.3 2,544,267 - IAI47_12010 0.80 +1.1 2,544,267 - IAI47_12010 0.80 +0.7 2,544,267 - IAI47_12010 0.80 -0.7 2,544,267 - IAI47_12010 0.80 +0.4 2,544,267 - IAI47_12010 0.80 +0.9 2,544,282 - IAI47_12010 0.84 -0.4 2,544,282 - IAI47_12010 0.84 -0.9 2,544,341 + -0.4 2,544,341 + +0.8 2,544,341 + +1.2 2,544,341 + -0.2 2,544,342 - +1.0
Or see this region's nucleotide sequence