Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_10150 and IAI47_10155 are separated by 49 nucleotides IAI47_10155 and IAI47_10160 overlap by 32 nucleotides IAI47_10160 and IAI47_10165 overlap by 4 nucleotides IAI47_10165 and IAI47_10170 are separated by 9 nucleotides IAI47_10170 and IAI47_10175 are separated by 94 nucleotides
IAI47_10150: IAI47_10150 - zinc-binding alcohol dehydrogenase family protein, at 2,147,882 to 2,148,817
_10150
IAI47_10155: IAI47_10155 - thiol-disulfide oxidoreductase DCC family protein, at 2,148,867 to 2,149,307
_10155
IAI47_10160: IAI47_10160 - AAA family ATPase, at 2,149,276 to 2,149,899
_10160
IAI47_10165: IAI47_10165 - PTS sugar transporter subunit IIA, at 2,149,896 to 2,150,333
_10165
IAI47_10170: IAI47_10170 - hypothetical protein, at 2,150,343 to 2,150,612
_10170
IAI47_10175: IAI47_10175 - class II fructose-bisphosphate aldolase, at 2,150,707 to 2,151,549
_10175
Position (kb)
2149
2150 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2148.387 kb on + strand, within IAI47_10150 at 2148.387 kb on + strand, within IAI47_10150 at 2148.388 kb on - strand, within IAI47_10150 at 2148.403 kb on + strand, within IAI47_10150 at 2148.403 kb on + strand, within IAI47_10150 at 2148.439 kb on + strand, within IAI47_10150 at 2148.439 kb on + strand, within IAI47_10150 at 2148.440 kb on - strand, within IAI47_10150 at 2148.494 kb on + strand, within IAI47_10150 at 2148.494 kb on + strand, within IAI47_10150 at 2148.494 kb on + strand, within IAI47_10150 at 2148.495 kb on - strand, within IAI47_10150 at 2148.495 kb on - strand, within IAI47_10150 at 2148.495 kb on - strand, within IAI47_10150 at 2148.495 kb on - strand, within IAI47_10150 at 2148.699 kb on + strand, within IAI47_10150 at 2148.699 kb on + strand, within IAI47_10150 at 2148.700 kb on - strand, within IAI47_10150 at 2148.702 kb on + strand, within IAI47_10150 at 2148.702 kb on + strand, within IAI47_10150 at 2148.702 kb on + strand, within IAI47_10150 at 2148.703 kb on - strand, within IAI47_10150 at 2148.703 kb on - strand, within IAI47_10150 at 2148.703 kb on - strand, within IAI47_10150 at 2148.766 kb on + strand at 2148.766 kb on + strand at 2148.766 kb on + strand at 2148.767 kb on - strand at 2148.808 kb on + strand at 2148.808 kb on + strand at 2148.809 kb on - strand at 2148.809 kb on - strand at 2148.815 kb on + strand at 2148.816 kb on - strand at 2148.816 kb on - strand at 2148.816 kb on - strand at 2148.935 kb on + strand, within IAI47_10155 at 2148.935 kb on + strand, within IAI47_10155 at 2148.937 kb on + strand, within IAI47_10155 at 2148.938 kb on - strand, within IAI47_10155 at 2148.953 kb on + strand, within IAI47_10155 at 2148.953 kb on + strand, within IAI47_10155 at 2148.953 kb on + strand, within IAI47_10155 at 2148.954 kb on - strand, within IAI47_10155 at 2148.954 kb on - strand, within IAI47_10155 at 2148.956 kb on - strand, within IAI47_10155 at 2148.979 kb on + strand, within IAI47_10155 at 2148.980 kb on - strand, within IAI47_10155 at 2148.989 kb on + strand, within IAI47_10155 at 2148.990 kb on - strand, within IAI47_10155 at 2149.056 kb on - strand, within IAI47_10155 at 2149.056 kb on - strand, within IAI47_10155 at 2149.095 kb on - strand, within IAI47_10155 at 2149.105 kb on + strand, within IAI47_10155 at 2149.105 kb on + strand, within IAI47_10155 at 2149.106 kb on - strand, within IAI47_10155 at 2149.106 kb on - strand, within IAI47_10155 at 2149.121 kb on + strand, within IAI47_10155 at 2149.121 kb on + strand, within IAI47_10155 at 2149.122 kb on - strand, within IAI47_10155 at 2149.122 kb on - strand, within IAI47_10155 at 2149.122 kb on - strand, within IAI47_10155 at 2149.122 kb on - strand, within IAI47_10155 at 2149.238 kb on + strand, within IAI47_10155 at 2149.247 kb on + strand, within IAI47_10155 at 2149.248 kb on - strand, within IAI47_10155 at 2149.248 kb on - strand, within IAI47_10155 at 2149.262 kb on - strand, within IAI47_10155 at 2149.292 kb on - strand at 2149.346 kb on + strand, within IAI47_10160 at 2149.482 kb on - strand, within IAI47_10160 at 2149.482 kb on - strand, within IAI47_10160 at 2149.529 kb on + strand, within IAI47_10160 at 2149.703 kb on + strand, within IAI47_10160 at 2149.704 kb on - strand, within IAI47_10160 at 2149.761 kb on + strand, within IAI47_10160 at 2149.761 kb on + strand, within IAI47_10160 at 2149.761 kb on + strand, within IAI47_10160 at 2149.761 kb on + strand, within IAI47_10160 at 2149.762 kb on - strand, within IAI47_10160 at 2149.762 kb on - strand, within IAI47_10160 at 2149.762 kb on - strand, within IAI47_10160 at 2149.888 kb on - strand at 2149.899 kb on + strand at 2149.899 kb on + strand at 2149.899 kb on + strand at 2149.899 kb on + strand at 2149.899 kb on + strand at 2149.900 kb on - strand at 2149.900 kb on - strand at 2150.030 kb on + strand, within IAI47_10165 at 2150.031 kb on - strand, within IAI47_10165 at 2150.048 kb on + strand, within IAI47_10165 at 2150.048 kb on + strand, within IAI47_10165 at 2150.049 kb on - strand, within IAI47_10165 at 2150.049 kb on - strand, within IAI47_10165 at 2150.049 kb on - strand, within IAI47_10165 at 2150.090 kb on + strand, within IAI47_10165 at 2150.091 kb on - strand, within IAI47_10165 at 2150.161 kb on + strand, within IAI47_10165 at 2150.161 kb on + strand, within IAI47_10165 at 2150.161 kb on + strand, within IAI47_10165 at 2150.161 kb on + strand, within IAI47_10165 at 2150.161 kb on + strand, within IAI47_10165 at 2150.161 kb on + strand, within IAI47_10165 at 2150.161 kb on + strand, within IAI47_10165 at 2150.161 kb on + strand, within IAI47_10165 at 2150.162 kb on - strand, within IAI47_10165 at 2150.162 kb on - strand, within IAI47_10165 at 2150.164 kb on + strand, within IAI47_10165 at 2150.164 kb on + strand, within IAI47_10165 at 2150.164 kb on + strand, within IAI47_10165 at 2150.164 kb on + strand, within IAI47_10165 at 2150.164 kb on + strand, within IAI47_10165 at 2150.165 kb on - strand, within IAI47_10165 at 2150.165 kb on - strand, within IAI47_10165 at 2150.165 kb on - strand, within IAI47_10165 at 2150.206 kb on + strand, within IAI47_10165 at 2150.221 kb on + strand, within IAI47_10165 at 2150.316 kb on + strand at 2150.317 kb on - strand at 2150.324 kb on + strand at 2150.325 kb on - strand at 2150.370 kb on + strand, within IAI47_10170 at 2150.370 kb on + strand, within IAI47_10170 at 2150.370 kb on + strand, within IAI47_10170 at 2150.371 kb on - strand, within IAI47_10170 at 2150.371 kb on - strand, within IAI47_10170 at 2150.408 kb on + strand, within IAI47_10170 at 2150.414 kb on - strand, within IAI47_10170 at 2150.433 kb on + strand, within IAI47_10170 at 2150.451 kb on - strand, within IAI47_10170 at 2150.451 kb on - strand, within IAI47_10170 at 2150.452 kb on + strand, within IAI47_10170 at 2150.453 kb on - strand, within IAI47_10170 at 2150.453 kb on - strand, within IAI47_10170 at 2150.453 kb on - strand, within IAI47_10170 at 2150.453 kb on - strand, within IAI47_10170 at 2150.462 kb on + strand, within IAI47_10170 at 2150.463 kb on + strand, within IAI47_10170 at 2150.463 kb on + strand, within IAI47_10170 at 2150.463 kb on + strand, within IAI47_10170 at 2150.464 kb on - strand, within IAI47_10170 at 2150.464 kb on - strand, within IAI47_10170 at 2150.464 kb on - strand, within IAI47_10170 at 2150.612 kb on + strand at 2150.612 kb on + strand at 2150.613 kb on - strand at 2150.613 kb on - strand at 2150.629 kb on + strand at 2150.629 kb on + strand at 2150.629 kb on + strand at 2150.630 kb on - strand at 2150.630 kb on - strand at 2150.630 kb on - strand at 2150.630 kb on - strand at 2150.630 kb on - strand at 2150.630 kb on - strand at 2150.631 kb on + strand at 2150.632 kb on - strand at 2150.632 kb on - strand at 2150.632 kb on - strand at 2150.632 kb on - strand at 2150.652 kb on - strand at 2150.652 kb on - strand at 2150.654 kb on + strand at 2150.664 kb on - strand at 2150.704 kb on + strand at 2150.705 kb on - strand at 2150.716 kb on + strand at 2150.717 kb on - strand at 2150.732 kb on - strand at 2150.732 kb on - strand at 2150.776 kb on - strand at 2150.882 kb on - strand, within IAI47_10175 at 2150.895 kb on + strand, within IAI47_10175 at 2150.896 kb on - strand, within IAI47_10175 at 2150.896 kb on - strand, within IAI47_10175
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,148,387 + IAI47_10150 0.54 +2.1 2,148,387 + IAI47_10150 0.54 +1.4 2,148,388 - IAI47_10150 0.54 +0.8 2,148,403 + IAI47_10150 0.56 +0.3 2,148,403 + IAI47_10150 0.56 +0.3 2,148,439 + IAI47_10150 0.60 +0.8 2,148,439 + IAI47_10150 0.60 -1.2 2,148,440 - IAI47_10150 0.60 -1.3 2,148,494 + IAI47_10150 0.65 +1.4 2,148,494 + IAI47_10150 0.65 +1.4 2,148,494 + IAI47_10150 0.65 +0.8 2,148,495 - IAI47_10150 0.65 +0.8 2,148,495 - IAI47_10150 0.65 -0.6 2,148,495 - IAI47_10150 0.65 +2.1 2,148,495 - IAI47_10150 0.65 -0.4 2,148,699 + IAI47_10150 0.87 +0.7 2,148,699 + IAI47_10150 0.87 -0.5 2,148,700 - IAI47_10150 0.87 -0.0 2,148,702 + IAI47_10150 0.88 +0.0 2,148,702 + IAI47_10150 0.88 +0.8 2,148,702 + IAI47_10150 0.88 -0.3 2,148,703 - IAI47_10150 0.88 +0.9 2,148,703 - IAI47_10150 0.88 -0.0 2,148,703 - IAI47_10150 0.88 -1.2 2,148,766 + +0.8 2,148,766 + -0.4 2,148,766 + -0.4 2,148,767 - -0.5 2,148,808 + +0.3 2,148,808 + +0.1 2,148,809 - +1.1 2,148,809 - +3.4 2,148,815 + -0.8 2,148,816 - -0.2 2,148,816 - +0.4 2,148,816 - +0.2 2,148,935 + IAI47_10155 0.15 -2.3 2,148,935 + IAI47_10155 0.15 -0.4 2,148,937 + IAI47_10155 0.16 +2.2 2,148,938 - IAI47_10155 0.16 -0.2 2,148,953 + IAI47_10155 0.20 +0.3 2,148,953 + IAI47_10155 0.20 -1.0 2,148,953 + IAI47_10155 0.20 -1.8 2,148,954 - IAI47_10155 0.20 +0.8 2,148,954 - IAI47_10155 0.20 +1.2 2,148,956 - IAI47_10155 0.20 +1.6 2,148,979 + IAI47_10155 0.25 -0.5 2,148,980 - IAI47_10155 0.26 -1.7 2,148,989 + IAI47_10155 0.28 -0.5 2,148,990 - IAI47_10155 0.28 +0.8 2,149,056 - IAI47_10155 0.43 -0.1 2,149,056 - IAI47_10155 0.43 -0.2 2,149,095 - IAI47_10155 0.52 +0.3 2,149,105 + IAI47_10155 0.54 +1.4 2,149,105 + IAI47_10155 0.54 +1.2 2,149,106 - IAI47_10155 0.54 +0.5 2,149,106 - IAI47_10155 0.54 -1.1 2,149,121 + IAI47_10155 0.58 +0.4 2,149,121 + IAI47_10155 0.58 -2.2 2,149,122 - IAI47_10155 0.58 +1.4 2,149,122 - IAI47_10155 0.58 +0.3 2,149,122 - IAI47_10155 0.58 -0.5 2,149,122 - IAI47_10155 0.58 +1.2 2,149,238 + IAI47_10155 0.84 -0.0 2,149,247 + IAI47_10155 0.86 -1.1 2,149,248 - IAI47_10155 0.86 +1.0 2,149,248 - IAI47_10155 0.86 -0.2 2,149,262 - IAI47_10155 0.90 +0.8 2,149,292 - -1.5 2,149,346 + IAI47_10160 0.11 -0.0 2,149,482 - IAI47_10160 0.33 +2.3 2,149,482 - IAI47_10160 0.33 +0.9 2,149,529 + IAI47_10160 0.41 -0.2 2,149,703 + IAI47_10160 0.68 +1.2 2,149,704 - IAI47_10160 0.69 -0.2 2,149,761 + IAI47_10160 0.78 +1.6 2,149,761 + IAI47_10160 0.78 +2.0 2,149,761 + IAI47_10160 0.78 +0.8 2,149,761 + IAI47_10160 0.78 +0.9 2,149,762 - IAI47_10160 0.78 -0.1 2,149,762 - IAI47_10160 0.78 +0.9 2,149,762 - IAI47_10160 0.78 -1.0 2,149,888 - -1.2 2,149,899 + -0.8 2,149,899 + +1.9 2,149,899 + -0.4 2,149,899 + -0.1 2,149,899 + +0.6 2,149,900 - +0.6 2,149,900 - +1.0 2,150,030 + IAI47_10165 0.31 -0.3 2,150,031 - IAI47_10165 0.31 -1.0 2,150,048 + IAI47_10165 0.35 +0.8 2,150,048 + IAI47_10165 0.35 -0.5 2,150,049 - IAI47_10165 0.35 +0.3 2,150,049 - IAI47_10165 0.35 -0.8 2,150,049 - IAI47_10165 0.35 -1.0 2,150,090 + IAI47_10165 0.44 +0.2 2,150,091 - IAI47_10165 0.45 -0.5 2,150,161 + IAI47_10165 0.61 +0.5 2,150,161 + IAI47_10165 0.61 +1.1 2,150,161 + IAI47_10165 0.61 -1.2 2,150,161 + IAI47_10165 0.61 +0.2 2,150,161 + IAI47_10165 0.61 -1.2 2,150,161 + IAI47_10165 0.61 +0.4 2,150,161 + IAI47_10165 0.61 +0.2 2,150,161 + IAI47_10165 0.61 -0.3 2,150,162 - IAI47_10165 0.61 -1.3 2,150,162 - IAI47_10165 0.61 -1.3 2,150,164 + IAI47_10165 0.61 -0.3 2,150,164 + IAI47_10165 0.61 -0.9 2,150,164 + IAI47_10165 0.61 +1.2 2,150,164 + IAI47_10165 0.61 -0.7 2,150,164 + IAI47_10165 0.61 -1.8 2,150,165 - IAI47_10165 0.61 +0.4 2,150,165 - IAI47_10165 0.61 -0.7 2,150,165 - IAI47_10165 0.61 +1.2 2,150,206 + IAI47_10165 0.71 -0.4 2,150,221 + IAI47_10165 0.74 +0.3 2,150,316 + -0.4 2,150,317 - +0.2 2,150,324 + -0.4 2,150,325 - -0.2 2,150,370 + IAI47_10170 0.10 -0.4 2,150,370 + IAI47_10170 0.10 -0.4 2,150,370 + IAI47_10170 0.10 +1.5 2,150,371 - IAI47_10170 0.10 -0.8 2,150,371 - IAI47_10170 0.10 +2.4 2,150,408 + IAI47_10170 0.24 -0.6 2,150,414 - IAI47_10170 0.26 -0.3 2,150,433 + IAI47_10170 0.33 -1.0 2,150,451 - IAI47_10170 0.40 +1.0 2,150,451 - IAI47_10170 0.40 -0.3 2,150,452 + IAI47_10170 0.40 -1.1 2,150,453 - IAI47_10170 0.41 -1.3 2,150,453 - IAI47_10170 0.41 +0.2 2,150,453 - IAI47_10170 0.41 -0.5 2,150,453 - IAI47_10170 0.41 +0.3 2,150,462 + IAI47_10170 0.44 -0.0 2,150,463 + IAI47_10170 0.44 -0.7 2,150,463 + IAI47_10170 0.44 -1.1 2,150,463 + IAI47_10170 0.44 -0.8 2,150,464 - IAI47_10170 0.45 -1.2 2,150,464 - IAI47_10170 0.45 +0.2 2,150,464 - IAI47_10170 0.45 +0.1 2,150,612 + +1.0 2,150,612 + +0.1 2,150,613 - -0.9 2,150,613 - -0.5 2,150,629 + -0.6 2,150,629 + +1.4 2,150,629 + +0.4 2,150,630 - +1.0 2,150,630 - +1.0 2,150,630 - +0.2 2,150,630 - +0.3 2,150,630 - +0.7 2,150,630 - -0.2 2,150,631 + -0.4 2,150,632 - -0.7 2,150,632 - -0.1 2,150,632 - +0.2 2,150,632 - +0.1 2,150,652 - +0.2 2,150,652 - -0.1 2,150,654 + +1.3 2,150,664 - -1.0 2,150,704 + -0.6 2,150,705 - -2.3 2,150,716 + -2.6 2,150,717 - -1.8 2,150,732 - -1.5 2,150,732 - -0.9 2,150,776 - +0.7 2,150,882 - IAI47_10175 0.21 +2.4 2,150,895 + IAI47_10175 0.22 -0.7 2,150,896 - IAI47_10175 0.22 -1.0 2,150,896 - IAI47_10175 0.22 +0.6
Or see this region's nucleotide sequence