Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20840 and IAI47_20845 are separated by 435 nucleotides IAI47_20845 and IAI47_20850 are separated by 175 nucleotides IAI47_20850 and IAI47_20855 are separated by 128 nucleotides
IAI47_20840: IAI47_20840 - Lrp/AsnC family transcriptional regulator, at 412,057 to 412,530
_20840
IAI47_20845: IAI47_20845 - SDR family oxidoreductase, at 412,966 to 413,766
_20845
IAI47_20850: IAI47_20850 - TetR/AcrR family transcriptional regulator, at 413,942 to 414,490
_20850
IAI47_20855: IAI47_20855 - alkene reductase, at 414,619 to 415,710
_20855
Position (kb) 412
413
414 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 411.978 kb on - strand at 411.981 kb on + strand at 411.982 kb on - strand at 411.982 kb on - strand at 411.983 kb on + strand at 411.984 kb on - strand at 412.038 kb on + strand at 412.077 kb on + strand at 412.077 kb on + strand at 412.077 kb on + strand at 412.078 kb on - strand at 412.085 kb on + strand at 412.086 kb on - strand at 412.142 kb on + strand, within IAI47_20840 at 412.142 kb on + strand, within IAI47_20840 at 412.142 kb on + strand, within IAI47_20840 at 412.220 kb on + strand, within IAI47_20840 at 412.220 kb on + strand, within IAI47_20840 at 412.348 kb on + strand, within IAI47_20840 at 412.348 kb on + strand, within IAI47_20840 at 412.348 kb on + strand, within IAI47_20840 at 412.348 kb on + strand, within IAI47_20840 at 412.349 kb on - strand, within IAI47_20840 at 412.354 kb on - strand, within IAI47_20840 at 412.408 kb on + strand, within IAI47_20840 at 412.504 kb on + strand at 412.505 kb on - strand at 412.505 kb on - strand at 412.509 kb on + strand at 412.509 kb on + strand at 412.510 kb on - strand at 412.510 kb on - strand at 412.510 kb on - strand at 412.510 kb on - strand at 412.513 kb on - strand at 412.513 kb on - strand at 412.528 kb on + strand at 412.528 kb on + strand at 412.528 kb on + strand at 412.529 kb on - strand at 412.529 kb on - strand at 412.601 kb on + strand at 412.787 kb on + strand at 412.787 kb on + strand at 412.787 kb on + strand at 412.788 kb on - strand at 412.807 kb on - strand at 412.808 kb on + strand at 412.809 kb on - strand at 412.809 kb on - strand at 412.809 kb on - strand at 412.813 kb on + strand at 412.853 kb on - strand at 412.942 kb on + strand at 412.943 kb on - strand at 412.943 kb on - strand at 412.943 kb on - strand at 412.954 kb on + strand at 412.954 kb on + strand at 412.955 kb on - strand at 413.073 kb on + strand, within IAI47_20845 at 413.074 kb on - strand, within IAI47_20845 at 413.074 kb on - strand, within IAI47_20845 at 413.077 kb on + strand, within IAI47_20845 at 413.077 kb on + strand, within IAI47_20845 at 413.077 kb on + strand, within IAI47_20845 at 413.077 kb on + strand, within IAI47_20845 at 413.077 kb on + strand, within IAI47_20845 at 413.077 kb on + strand, within IAI47_20845 at 413.078 kb on - strand, within IAI47_20845 at 413.078 kb on - strand, within IAI47_20845 at 413.151 kb on + strand, within IAI47_20845 at 413.151 kb on + strand, within IAI47_20845 at 413.162 kb on + strand, within IAI47_20845 at 413.163 kb on - strand, within IAI47_20845 at 413.180 kb on + strand, within IAI47_20845 at 413.180 kb on + strand, within IAI47_20845 at 413.181 kb on - strand, within IAI47_20845 at 413.181 kb on - strand, within IAI47_20845 at 413.181 kb on - strand, within IAI47_20845 at 413.301 kb on + strand, within IAI47_20845 at 413.301 kb on + strand, within IAI47_20845 at 413.302 kb on - strand, within IAI47_20845 at 413.302 kb on - strand, within IAI47_20845 at 413.304 kb on + strand, within IAI47_20845 at 413.304 kb on + strand, within IAI47_20845 at 413.304 kb on + strand, within IAI47_20845 at 413.304 kb on + strand, within IAI47_20845 at 413.407 kb on - strand, within IAI47_20845 at 413.412 kb on + strand, within IAI47_20845 at 413.412 kb on + strand, within IAI47_20845 at 413.412 kb on + strand, within IAI47_20845 at 413.413 kb on - strand, within IAI47_20845 at 413.413 kb on - strand, within IAI47_20845 at 413.413 kb on - strand, within IAI47_20845 at 413.434 kb on - strand, within IAI47_20845 at 413.434 kb on - strand, within IAI47_20845 at 413.434 kb on - strand, within IAI47_20845 at 413.434 kb on - strand, within IAI47_20845 at 413.458 kb on + strand, within IAI47_20845 at 413.459 kb on - strand, within IAI47_20845 at 413.459 kb on - strand, within IAI47_20845 at 413.459 kb on - strand, within IAI47_20845 at 413.459 kb on - strand, within IAI47_20845 at 413.459 kb on - strand, within IAI47_20845 at 413.459 kb on - strand, within IAI47_20845 at 413.610 kb on - strand, within IAI47_20845 at 413.687 kb on + strand at 413.687 kb on + strand at 413.687 kb on + strand at 413.688 kb on - strand at 413.695 kb on + strand at 413.695 kb on + strand at 413.696 kb on - strand at 413.714 kb on + strand at 413.714 kb on + strand at 413.714 kb on + strand at 413.714 kb on + strand at 413.715 kb on - strand at 413.715 kb on - strand at 413.715 kb on - strand at 413.715 kb on - strand at 413.718 kb on + strand at 413.719 kb on - strand at 413.719 kb on - strand at 413.740 kb on + strand at 413.740 kb on + strand at 413.740 kb on + strand at 413.740 kb on + strand at 413.741 kb on - strand at 413.803 kb on + strand at 413.803 kb on + strand at 413.803 kb on + strand at 413.803 kb on + strand at 413.803 kb on + strand at 413.803 kb on + strand at 413.803 kb on + strand at 413.803 kb on + strand at 413.803 kb on + strand at 413.803 kb on + strand at 413.803 kb on + strand at 413.804 kb on - strand at 413.804 kb on - strand at 413.804 kb on - strand at 413.804 kb on - strand at 413.804 kb on - strand at 413.804 kb on - strand at 413.804 kb on - strand at 413.804 kb on - strand at 413.805 kb on + strand at 413.805 kb on + strand at 413.805 kb on + strand at 413.805 kb on + strand at 413.806 kb on - strand at 413.806 kb on - strand at 413.819 kb on + strand at 413.819 kb on + strand at 413.863 kb on + strand at 413.865 kb on + strand at 413.866 kb on - strand at 413.866 kb on - strand at 413.866 kb on - strand at 413.871 kb on - strand at 413.871 kb on - strand at 413.917 kb on - strand at 413.917 kb on - strand at 413.965 kb on + strand at 413.965 kb on + strand at 413.966 kb on - strand at 413.966 kb on - strand at 414.068 kb on - strand, within IAI47_20850 at 414.212 kb on - strand, within IAI47_20850 at 414.238 kb on - strand, within IAI47_20850 at 414.238 kb on - strand, within IAI47_20850 at 414.238 kb on - strand, within IAI47_20850 at 414.240 kb on + strand, within IAI47_20850 at 414.332 kb on - strand, within IAI47_20850 at 414.347 kb on - strand, within IAI47_20850 at 414.349 kb on - strand, within IAI47_20850 at 414.366 kb on - strand, within IAI47_20850 at 414.366 kb on - strand, within IAI47_20850 at 414.366 kb on - strand, within IAI47_20850 at 414.399 kb on - strand, within IAI47_20850 at 414.409 kb on - strand, within IAI47_20850 at 414.409 kb on - strand, within IAI47_20850 at 414.495 kb on + strand at 414.495 kb on + strand at 414.496 kb on - strand at 414.496 kb on - strand at 414.496 kb on - strand at 414.496 kb on - strand at 414.518 kb on + strand at 414.538 kb on - strand at 414.586 kb on + strand at 414.599 kb on + strand at 414.599 kb on + strand at 414.599 kb on + strand at 414.646 kb on + strand at 414.646 kb on + strand at 414.646 kb on + strand at 414.646 kb on + strand at 414.680 kb on + strand at 414.680 kb on + strand at 414.680 kb on + strand at 414.681 kb on - strand at 414.681 kb on - strand at 414.748 kb on + strand, within IAI47_20855 at 414.748 kb on + strand, within IAI47_20855 at 414.749 kb on - strand, within IAI47_20855 at 414.751 kb on + strand, within IAI47_20855 at 414.751 kb on + strand, within IAI47_20855 at 414.751 kb on + strand, within IAI47_20855 at 414.751 kb on + strand, within IAI47_20855
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 411,978 - -1.1 411,981 + -1.4 411,982 - +0.7 411,982 - -0.2 411,983 + +0.7 411,984 - +0.9 412,038 + +1.3 412,077 + -0.1 412,077 + +0.5 412,077 + -1.6 412,078 - +0.7 412,085 + +0.1 412,086 - -1.6 412,142 + IAI47_20840 0.18 +1.3 412,142 + IAI47_20840 0.18 +0.8 412,142 + IAI47_20840 0.18 +0.5 412,220 + IAI47_20840 0.34 -0.7 412,220 + IAI47_20840 0.34 -0.2 412,348 + IAI47_20840 0.61 +0.1 412,348 + IAI47_20840 0.61 -0.2 412,348 + IAI47_20840 0.61 -0.6 412,348 + IAI47_20840 0.61 -0.4 412,349 - IAI47_20840 0.62 +0.3 412,354 - IAI47_20840 0.63 -2.5 412,408 + IAI47_20840 0.74 -0.5 412,504 + -0.7 412,505 - +1.1 412,505 - -0.0 412,509 + -2.0 412,509 + +1.1 412,510 - +2.4 412,510 - -2.2 412,510 - +0.1 412,510 - +0.9 412,513 - -0.3 412,513 - -0.1 412,528 + -1.0 412,528 + +1.6 412,528 + -3.0 412,529 - -1.3 412,529 - +1.4 412,601 + -1.6 412,787 + +0.1 412,787 + +0.5 412,787 + +0.2 412,788 - +0.9 412,807 - +1.0 412,808 + -0.3 412,809 - +0.1 412,809 - +0.5 412,809 - -0.7 412,813 + -1.4 412,853 - +0.7 412,942 + -0.8 412,943 - -2.8 412,943 - +0.4 412,943 - +1.2 412,954 + +0.6 412,954 + -0.2 412,955 - +1.5 413,073 + IAI47_20845 0.13 +0.4 413,074 - IAI47_20845 0.13 -0.1 413,074 - IAI47_20845 0.13 -1.0 413,077 + IAI47_20845 0.14 -0.1 413,077 + IAI47_20845 0.14 +0.2 413,077 + IAI47_20845 0.14 +0.5 413,077 + IAI47_20845 0.14 -0.8 413,077 + IAI47_20845 0.14 +1.1 413,077 + IAI47_20845 0.14 -0.1 413,078 - IAI47_20845 0.14 -1.5 413,078 - IAI47_20845 0.14 +2.0 413,151 + IAI47_20845 0.23 -0.7 413,151 + IAI47_20845 0.23 +0.2 413,162 + IAI47_20845 0.24 -0.5 413,163 - IAI47_20845 0.25 -0.3 413,180 + IAI47_20845 0.27 +1.1 413,180 + IAI47_20845 0.27 -0.1 413,181 - IAI47_20845 0.27 -0.4 413,181 - IAI47_20845 0.27 +0.2 413,181 - IAI47_20845 0.27 +0.4 413,301 + IAI47_20845 0.42 +1.3 413,301 + IAI47_20845 0.42 -1.2 413,302 - IAI47_20845 0.42 -0.1 413,302 - IAI47_20845 0.42 -1.1 413,304 + IAI47_20845 0.42 +0.5 413,304 + IAI47_20845 0.42 -1.4 413,304 + IAI47_20845 0.42 -0.6 413,304 + IAI47_20845 0.42 -2.1 413,407 - IAI47_20845 0.55 -0.4 413,412 + IAI47_20845 0.56 +1.1 413,412 + IAI47_20845 0.56 +1.3 413,412 + IAI47_20845 0.56 -0.8 413,413 - IAI47_20845 0.56 -2.9 413,413 - IAI47_20845 0.56 -0.1 413,413 - IAI47_20845 0.56 +1.1 413,434 - IAI47_20845 0.58 -0.3 413,434 - IAI47_20845 0.58 +0.1 413,434 - IAI47_20845 0.58 -0.5 413,434 - IAI47_20845 0.58 -0.9 413,458 + IAI47_20845 0.61 +1.8 413,459 - IAI47_20845 0.62 +0.0 413,459 - IAI47_20845 0.62 +0.2 413,459 - IAI47_20845 0.62 -1.0 413,459 - IAI47_20845 0.62 +0.0 413,459 - IAI47_20845 0.62 -0.1 413,459 - IAI47_20845 0.62 +1.5 413,610 - IAI47_20845 0.80 -1.4 413,687 + +1.0 413,687 + -0.9 413,687 + -0.8 413,688 - -1.1 413,695 + -1.7 413,695 + -0.6 413,696 - -1.1 413,714 + +0.3 413,714 + -1.6 413,714 + +0.8 413,714 + -0.5 413,715 - +1.1 413,715 - -0.7 413,715 - -0.7 413,715 - +0.4 413,718 + +0.6 413,719 - -1.4 413,719 - -0.9 413,740 + +1.2 413,740 + -1.1 413,740 + -0.6 413,740 + -1.2 413,741 - +0.9 413,803 + +1.1 413,803 + -1.5 413,803 + +0.5 413,803 + +0.2 413,803 + +2.4 413,803 + +1.1 413,803 + -1.1 413,803 + +1.7 413,803 + -0.5 413,803 + -1.0 413,803 + +0.5 413,804 - -0.2 413,804 - -0.4 413,804 - -1.6 413,804 - +0.1 413,804 - -1.6 413,804 - -0.5 413,804 - -1.7 413,804 - -0.4 413,805 + -0.9 413,805 + +0.6 413,805 + +1.5 413,805 + +0.9 413,806 - -0.3 413,806 - +2.0 413,819 + -0.8 413,819 + +0.2 413,863 + +1.0 413,865 + -0.7 413,866 - +0.2 413,866 - -1.6 413,866 - +0.6 413,871 - -0.4 413,871 - -1.1 413,917 - -0.7 413,917 - +0.1 413,965 + +0.0 413,965 + +0.6 413,966 - +0.5 413,966 - -0.3 414,068 - IAI47_20850 0.23 -2.4 414,212 - IAI47_20850 0.49 +0.1 414,238 - IAI47_20850 0.54 +0.6 414,238 - IAI47_20850 0.54 +0.2 414,238 - IAI47_20850 0.54 -2.0 414,240 + IAI47_20850 0.54 +0.2 414,332 - IAI47_20850 0.71 +1.7 414,347 - IAI47_20850 0.74 +1.7 414,349 - IAI47_20850 0.74 -0.3 414,366 - IAI47_20850 0.77 +0.1 414,366 - IAI47_20850 0.77 +1.3 414,366 - IAI47_20850 0.77 -0.3 414,399 - IAI47_20850 0.83 -2.3 414,409 - IAI47_20850 0.85 +0.7 414,409 - IAI47_20850 0.85 -0.8 414,495 + -1.9 414,495 + -0.6 414,496 - -0.8 414,496 - +1.1 414,496 - +2.8 414,496 - +2.9 414,518 + -0.5 414,538 - -0.6 414,586 + +1.0 414,599 + +1.1 414,599 + +1.1 414,599 + -0.5 414,646 + +0.1 414,646 + +0.6 414,646 + +0.7 414,646 + +0.2 414,680 + -0.5 414,680 + -0.1 414,680 + +0.7 414,681 - -0.3 414,681 - +0.4 414,748 + IAI47_20855 0.12 -1.0 414,748 + IAI47_20855 0.12 +0.2 414,749 - IAI47_20855 0.12 +1.1 414,751 + IAI47_20855 0.12 +1.6 414,751 + IAI47_20855 0.12 +0.6 414,751 + IAI47_20855 0.12 -1.4 414,751 + IAI47_20855 0.12 -0.8
Or see this region's nucleotide sequence