Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20685 and IAI47_20690 are separated by 225 nucleotides IAI47_20690 and IAI47_20695 are separated by 9 nucleotides IAI47_20695 and IAI47_20700 are separated by 9 nucleotides
IAI47_20685: IAI47_20685 - thioredoxin domain-containing protein, at 386,684 to 387,226
_20685
IAI47_20690: IAI47_20690 - ASCH domain-containing protein, at 387,452 to 387,865
_20690
IAI47_20695: IAI47_20695 - HesA/MoeB/ThiF family protein, at 387,875 to 388,843
_20695
IAI47_20700: IAI47_20700 - thiazole synthase, at 388,853 to 389,608
_20700
Position (kb) 387
388 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 386.458 kb on + strand at 386.458 kb on + strand at 386.459 kb on - strand at 386.459 kb on - strand at 386.459 kb on - strand at 386.462 kb on + strand at 386.462 kb on + strand at 386.462 kb on + strand at 386.462 kb on + strand at 386.462 kb on + strand at 386.462 kb on + strand at 386.463 kb on - strand at 386.463 kb on - strand at 386.463 kb on - strand at 386.463 kb on - strand at 386.463 kb on - strand at 386.463 kb on - strand at 386.463 kb on - strand at 386.463 kb on - strand at 386.463 kb on - strand at 386.489 kb on + strand at 386.502 kb on - strand at 386.502 kb on - strand at 386.502 kb on - strand at 386.513 kb on + strand at 386.513 kb on + strand at 386.514 kb on - strand at 386.514 kb on - strand at 386.514 kb on - strand at 386.514 kb on - strand at 386.522 kb on + strand at 386.559 kb on - strand at 386.626 kb on - strand at 386.644 kb on + strand at 386.645 kb on - strand at 386.645 kb on - strand at 386.653 kb on + strand at 386.653 kb on + strand at 386.654 kb on - strand at 386.665 kb on + strand at 386.679 kb on + strand at 386.680 kb on - strand at 386.687 kb on + strand at 386.687 kb on + strand at 386.687 kb on + strand at 386.687 kb on + strand at 386.687 kb on + strand at 386.687 kb on + strand at 386.688 kb on - strand at 386.688 kb on - strand at 386.688 kb on - strand at 386.688 kb on - strand at 386.688 kb on - strand at 386.688 kb on - strand at 386.688 kb on - strand at 386.688 kb on - strand at 386.688 kb on - strand at 386.752 kb on + strand, within IAI47_20685 at 386.752 kb on + strand, within IAI47_20685 at 386.752 kb on + strand, within IAI47_20685 at 386.752 kb on + strand, within IAI47_20685 at 386.752 kb on + strand, within IAI47_20685 at 386.753 kb on - strand, within IAI47_20685 at 386.753 kb on - strand, within IAI47_20685 at 386.753 kb on - strand, within IAI47_20685 at 386.782 kb on + strand, within IAI47_20685 at 386.782 kb on + strand, within IAI47_20685 at 386.783 kb on - strand, within IAI47_20685 at 386.788 kb on - strand, within IAI47_20685 at 386.818 kb on + strand, within IAI47_20685 at 386.818 kb on + strand, within IAI47_20685 at 386.818 kb on + strand, within IAI47_20685 at 386.819 kb on - strand, within IAI47_20685 at 386.819 kb on - strand, within IAI47_20685 at 386.864 kb on + strand, within IAI47_20685 at 386.865 kb on - strand, within IAI47_20685 at 386.865 kb on - strand, within IAI47_20685 at 386.885 kb on + strand, within IAI47_20685 at 386.886 kb on - strand, within IAI47_20685 at 386.886 kb on - strand, within IAI47_20685 at 386.886 kb on - strand, within IAI47_20685 at 386.886 kb on - strand, within IAI47_20685 at 386.886 kb on - strand, within IAI47_20685 at 386.887 kb on + strand, within IAI47_20685 at 386.887 kb on + strand, within IAI47_20685 at 386.887 kb on + strand, within IAI47_20685 at 386.888 kb on - strand, within IAI47_20685 at 386.888 kb on - strand, within IAI47_20685 at 386.888 kb on - strand, within IAI47_20685 at 386.888 kb on - strand, within IAI47_20685 at 386.912 kb on - strand, within IAI47_20685 at 387.031 kb on + strand, within IAI47_20685 at 387.032 kb on - strand, within IAI47_20685 at 387.038 kb on + strand, within IAI47_20685 at 387.038 kb on + strand, within IAI47_20685 at 387.039 kb on - strand, within IAI47_20685 at 387.039 kb on - strand, within IAI47_20685 at 387.039 kb on - strand, within IAI47_20685 at 387.048 kb on + strand, within IAI47_20685 at 387.048 kb on + strand, within IAI47_20685 at 387.048 kb on + strand, within IAI47_20685 at 387.049 kb on - strand, within IAI47_20685 at 387.049 kb on - strand, within IAI47_20685 at 387.049 kb on - strand, within IAI47_20685 at 387.094 kb on + strand, within IAI47_20685 at 387.095 kb on - strand, within IAI47_20685 at 387.110 kb on + strand, within IAI47_20685 at 387.110 kb on + strand, within IAI47_20685 at 387.110 kb on + strand, within IAI47_20685 at 387.110 kb on + strand, within IAI47_20685 at 387.111 kb on - strand, within IAI47_20685 at 387.111 kb on - strand, within IAI47_20685 at 387.111 kb on - strand, within IAI47_20685 at 387.111 kb on - strand, within IAI47_20685 at 387.111 kb on - strand, within IAI47_20685 at 387.111 kb on - strand, within IAI47_20685 at 387.111 kb on - strand, within IAI47_20685 at 387.111 kb on - strand, within IAI47_20685 at 387.111 kb on - strand, within IAI47_20685 at 387.111 kb on - strand, within IAI47_20685 at 387.111 kb on - strand, within IAI47_20685 at 387.128 kb on - strand, within IAI47_20685 at 387.148 kb on + strand, within IAI47_20685 at 387.148 kb on + strand, within IAI47_20685 at 387.149 kb on - strand, within IAI47_20685 at 387.165 kb on + strand, within IAI47_20685 at 387.166 kb on - strand, within IAI47_20685 at 387.166 kb on - strand, within IAI47_20685 at 387.225 kb on - strand at 387.230 kb on + strand at 387.230 kb on + strand at 387.231 kb on - strand at 387.263 kb on + strand at 387.264 kb on - strand at 387.311 kb on + strand at 387.311 kb on + strand at 387.312 kb on - strand at 387.315 kb on + strand at 387.315 kb on + strand at 387.315 kb on + strand at 387.315 kb on + strand at 387.315 kb on + strand at 387.315 kb on + strand at 387.315 kb on + strand at 387.315 kb on + strand at 387.316 kb on - strand at 387.316 kb on - strand at 387.316 kb on - strand at 387.316 kb on - strand at 387.316 kb on - strand at 387.316 kb on - strand at 387.316 kb on - strand at 387.357 kb on + strand at 387.358 kb on - strand at 387.358 kb on - strand at 387.360 kb on + strand at 387.360 kb on + strand at 387.360 kb on + strand at 387.381 kb on + strand at 387.381 kb on + strand at 387.382 kb on - strand at 387.382 kb on - strand at 387.391 kb on - strand at 387.414 kb on + strand at 387.414 kb on + strand at 387.414 kb on + strand at 387.415 kb on - strand at 387.415 kb on - strand at 387.431 kb on + strand at 387.432 kb on - strand at 387.474 kb on + strand at 387.474 kb on + strand at 387.475 kb on - strand at 387.475 kb on - strand at 387.475 kb on - strand at 387.499 kb on + strand, within IAI47_20690 at 387.500 kb on - strand, within IAI47_20690 at 387.500 kb on - strand, within IAI47_20690 at 387.529 kb on - strand, within IAI47_20690 at 387.575 kb on - strand, within IAI47_20690 at 387.691 kb on - strand, within IAI47_20690 at 387.691 kb on - strand, within IAI47_20690 at 387.765 kb on - strand, within IAI47_20690 at 387.788 kb on + strand, within IAI47_20690 at 387.788 kb on + strand, within IAI47_20690 at 387.788 kb on + strand, within IAI47_20690 at 387.788 kb on + strand, within IAI47_20690 at 387.789 kb on - strand, within IAI47_20690 at 387.806 kb on + strand, within IAI47_20690 at 387.806 kb on + strand, within IAI47_20690 at 387.807 kb on - strand, within IAI47_20690 at 387.863 kb on + strand at 387.877 kb on - strand at 387.877 kb on - strand at 387.884 kb on - strand at 387.884 kb on - strand at 387.884 kb on - strand at 387.884 kb on - strand at 388.010 kb on + strand, within IAI47_20695 at 388.010 kb on + strand, within IAI47_20695 at 388.010 kb on + strand, within IAI47_20695 at 388.010 kb on + strand, within IAI47_20695 at 388.040 kb on - strand, within IAI47_20695 at 388.073 kb on - strand, within IAI47_20695 at 388.073 kb on - strand, within IAI47_20695 at 388.073 kb on - strand, within IAI47_20695 at 388.073 kb on - strand, within IAI47_20695 at 388.073 kb on - strand, within IAI47_20695 at 388.120 kb on + strand, within IAI47_20695 at 388.120 kb on + strand, within IAI47_20695 at 388.239 kb on - strand, within IAI47_20695 at 388.239 kb on - strand, within IAI47_20695 at 388.239 kb on - strand, within IAI47_20695 at 388.241 kb on + strand, within IAI47_20695 at 388.241 kb on + strand, within IAI47_20695 at 388.241 kb on + strand, within IAI47_20695 at 388.241 kb on + strand, within IAI47_20695 at 388.241 kb on + strand, within IAI47_20695 at 388.242 kb on - strand, within IAI47_20695 at 388.242 kb on - strand, within IAI47_20695 at 388.242 kb on - strand, within IAI47_20695 at 388.242 kb on - strand, within IAI47_20695 at 388.242 kb on - strand, within IAI47_20695 at 388.242 kb on - strand, within IAI47_20695 at 388.298 kb on + strand, within IAI47_20695 at 388.298 kb on + strand, within IAI47_20695 at 388.298 kb on + strand, within IAI47_20695 at 388.319 kb on - strand, within IAI47_20695 at 388.389 kb on + strand, within IAI47_20695 at 388.389 kb on + strand, within IAI47_20695 at 388.389 kb on + strand, within IAI47_20695 at 388.389 kb on + strand, within IAI47_20695 at 388.390 kb on - strand, within IAI47_20695 at 388.390 kb on - strand, within IAI47_20695 at 388.390 kb on - strand, within IAI47_20695 at 388.396 kb on + strand, within IAI47_20695 at 388.396 kb on + strand, within IAI47_20695 at 388.397 kb on - strand, within IAI47_20695 at 388.461 kb on + strand, within IAI47_20695 at 388.462 kb on - strand, within IAI47_20695 at 388.462 kb on - strand, within IAI47_20695 at 388.462 kb on - strand, within IAI47_20695 at 388.462 kb on - strand, within IAI47_20695 at 388.462 kb on - strand, within IAI47_20695 at 388.465 kb on + strand, within IAI47_20695 at 388.465 kb on + strand, within IAI47_20695 at 388.465 kb on + strand, within IAI47_20695 at 388.465 kb on + strand, within IAI47_20695 at 388.466 kb on - strand, within IAI47_20695 at 388.466 kb on - strand, within IAI47_20695 at 388.466 kb on - strand, within IAI47_20695 at 388.466 kb on - strand, within IAI47_20695 at 388.466 kb on - strand, within IAI47_20695 at 388.523 kb on + strand, within IAI47_20695 at 388.612 kb on + strand, within IAI47_20695 at 388.687 kb on - strand, within IAI47_20695 at 388.697 kb on - strand, within IAI47_20695
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 386,458 + +0.0 386,458 + +1.9 386,459 - +1.1 386,459 - +2.3 386,459 - -1.4 386,462 + -1.4 386,462 + -1.9 386,462 + -0.5 386,462 + +1.5 386,462 + -1.1 386,462 + +0.3 386,463 - +0.9 386,463 - -0.0 386,463 - -1.7 386,463 - -1.3 386,463 - +0.1 386,463 - +0.0 386,463 - -0.0 386,463 - -0.9 386,463 - -0.4 386,489 + -0.7 386,502 - +1.3 386,502 - -1.0 386,502 - -2.1 386,513 + -0.5 386,513 + -0.3 386,514 - -1.5 386,514 - -2.7 386,514 - +0.5 386,514 - +0.2 386,522 + +1.2 386,559 - -0.3 386,626 - -1.3 386,644 + +0.7 386,645 - -0.5 386,645 - -0.8 386,653 + +0.1 386,653 + +0.8 386,654 - +0.6 386,665 + +0.2 386,679 + -1.5 386,680 - -0.3 386,687 + +0.4 386,687 + +0.0 386,687 + +0.2 386,687 + -0.5 386,687 + -1.1 386,687 + +1.8 386,688 - +0.7 386,688 - +0.1 386,688 - -0.3 386,688 - -2.8 386,688 - -0.1 386,688 - -1.6 386,688 - -0.5 386,688 - +0.3 386,688 - +0.3 386,752 + IAI47_20685 0.13 -0.5 386,752 + IAI47_20685 0.13 +0.6 386,752 + IAI47_20685 0.13 +1.9 386,752 + IAI47_20685 0.13 +0.6 386,752 + IAI47_20685 0.13 -1.8 386,753 - IAI47_20685 0.13 +0.4 386,753 - IAI47_20685 0.13 +0.8 386,753 - IAI47_20685 0.13 -0.2 386,782 + IAI47_20685 0.18 -2.8 386,782 + IAI47_20685 0.18 -0.9 386,783 - IAI47_20685 0.18 -0.3 386,788 - IAI47_20685 0.19 +0.3 386,818 + IAI47_20685 0.25 +1.1 386,818 + IAI47_20685 0.25 +0.4 386,818 + IAI47_20685 0.25 -0.1 386,819 - IAI47_20685 0.25 +2.3 386,819 - IAI47_20685 0.25 +0.3 386,864 + IAI47_20685 0.33 +0.0 386,865 - IAI47_20685 0.33 +0.6 386,865 - IAI47_20685 0.33 -0.1 386,885 + IAI47_20685 0.37 +0.7 386,886 - IAI47_20685 0.37 -1.3 386,886 - IAI47_20685 0.37 -3.1 386,886 - IAI47_20685 0.37 -0.4 386,886 - IAI47_20685 0.37 +0.7 386,886 - IAI47_20685 0.37 +1.3 386,887 + IAI47_20685 0.37 -0.5 386,887 + IAI47_20685 0.37 +0.2 386,887 + IAI47_20685 0.37 +0.2 386,888 - IAI47_20685 0.38 -0.7 386,888 - IAI47_20685 0.38 -0.4 386,888 - IAI47_20685 0.38 -0.5 386,888 - IAI47_20685 0.38 +1.7 386,912 - IAI47_20685 0.42 -0.5 387,031 + IAI47_20685 0.64 +2.3 387,032 - IAI47_20685 0.64 +0.0 387,038 + IAI47_20685 0.65 +0.6 387,038 + IAI47_20685 0.65 +0.0 387,039 - IAI47_20685 0.65 -1.3 387,039 - IAI47_20685 0.65 +1.5 387,039 - IAI47_20685 0.65 -1.5 387,048 + IAI47_20685 0.67 -1.0 387,048 + IAI47_20685 0.67 +0.2 387,048 + IAI47_20685 0.67 +0.8 387,049 - IAI47_20685 0.67 -0.4 387,049 - IAI47_20685 0.67 -1.6 387,049 - IAI47_20685 0.67 +2.0 387,094 + IAI47_20685 0.76 +1.2 387,095 - IAI47_20685 0.76 -1.5 387,110 + IAI47_20685 0.78 +0.9 387,110 + IAI47_20685 0.78 -0.1 387,110 + IAI47_20685 0.78 +1.4 387,110 + IAI47_20685 0.78 -0.1 387,111 - IAI47_20685 0.79 -1.0 387,111 - IAI47_20685 0.79 +1.5 387,111 - IAI47_20685 0.79 +0.5 387,111 - IAI47_20685 0.79 +0.8 387,111 - IAI47_20685 0.79 +0.3 387,111 - IAI47_20685 0.79 +1.3 387,111 - IAI47_20685 0.79 -1.8 387,111 - IAI47_20685 0.79 -0.4 387,111 - IAI47_20685 0.79 +1.0 387,111 - IAI47_20685 0.79 -0.1 387,111 - IAI47_20685 0.79 +3.3 387,128 - IAI47_20685 0.82 -1.3 387,148 + IAI47_20685 0.85 -2.3 387,148 + IAI47_20685 0.85 +1.6 387,149 - IAI47_20685 0.86 +0.4 387,165 + IAI47_20685 0.89 -0.1 387,166 - IAI47_20685 0.89 +0.7 387,166 - IAI47_20685 0.89 -2.8 387,225 - -0.2 387,230 + -1.7 387,230 + +0.6 387,231 - -0.5 387,263 + +0.4 387,264 - -1.5 387,311 + +0.1 387,311 + -1.8 387,312 - +0.3 387,315 + -1.0 387,315 + -0.2 387,315 + -0.6 387,315 + +0.6 387,315 + -1.7 387,315 + -1.5 387,315 + +0.1 387,315 + +0.0 387,316 - +0.3 387,316 - -1.4 387,316 - -2.1 387,316 - -1.0 387,316 - +2.0 387,316 - +1.3 387,316 - -0.5 387,357 + +0.2 387,358 - -1.1 387,358 - +0.0 387,360 + +0.7 387,360 + +1.5 387,360 + +0.7 387,381 + +0.4 387,381 + +3.3 387,382 - -0.8 387,382 - +1.8 387,391 - -0.6 387,414 + +0.3 387,414 + -0.2 387,414 + -1.1 387,415 - -0.8 387,415 - -0.3 387,431 + -0.9 387,432 - -0.5 387,474 + +1.3 387,474 + +1.3 387,475 - +0.4 387,475 - -0.5 387,475 - +0.6 387,499 + IAI47_20690 0.11 -0.1 387,500 - IAI47_20690 0.12 +0.7 387,500 - IAI47_20690 0.12 +1.3 387,529 - IAI47_20690 0.19 -2.3 387,575 - IAI47_20690 0.30 -0.3 387,691 - IAI47_20690 0.58 +1.2 387,691 - IAI47_20690 0.58 -0.6 387,765 - IAI47_20690 0.76 +0.7 387,788 + IAI47_20690 0.81 -1.6 387,788 + IAI47_20690 0.81 -0.1 387,788 + IAI47_20690 0.81 -0.4 387,788 + IAI47_20690 0.81 +0.5 387,789 - IAI47_20690 0.81 +3.2 387,806 + IAI47_20690 0.86 +1.3 387,806 + IAI47_20690 0.86 +2.1 387,807 - IAI47_20690 0.86 +2.2 387,863 + -0.8 387,877 - -0.6 387,877 - -0.7 387,884 - +0.6 387,884 - +0.3 387,884 - +0.4 387,884 - -0.1 388,010 + IAI47_20695 0.14 +4.1 388,010 + IAI47_20695 0.14 +2.1 388,010 + IAI47_20695 0.14 +1.4 388,010 + IAI47_20695 0.14 +0.4 388,040 - IAI47_20695 0.17 +0.9 388,073 - IAI47_20695 0.20 -0.4 388,073 - IAI47_20695 0.20 +0.5 388,073 - IAI47_20695 0.20 -0.3 388,073 - IAI47_20695 0.20 +1.9 388,073 - IAI47_20695 0.20 -1.8 388,120 + IAI47_20695 0.25 -0.3 388,120 + IAI47_20695 0.25 +1.5 388,239 - IAI47_20695 0.38 +0.6 388,239 - IAI47_20695 0.38 +0.1 388,239 - IAI47_20695 0.38 +0.6 388,241 + IAI47_20695 0.38 -0.0 388,241 + IAI47_20695 0.38 +0.6 388,241 + IAI47_20695 0.38 -0.3 388,241 + IAI47_20695 0.38 -2.0 388,241 + IAI47_20695 0.38 +1.1 388,242 - IAI47_20695 0.38 -0.8 388,242 - IAI47_20695 0.38 +0.8 388,242 - IAI47_20695 0.38 +0.4 388,242 - IAI47_20695 0.38 +0.9 388,242 - IAI47_20695 0.38 +0.4 388,242 - IAI47_20695 0.38 +0.0 388,298 + IAI47_20695 0.44 +1.0 388,298 + IAI47_20695 0.44 +0.2 388,298 + IAI47_20695 0.44 +0.7 388,319 - IAI47_20695 0.46 +0.4 388,389 + IAI47_20695 0.53 -1.8 388,389 + IAI47_20695 0.53 -2.4 388,389 + IAI47_20695 0.53 +1.0 388,389 + IAI47_20695 0.53 -0.6 388,390 - IAI47_20695 0.53 +1.0 388,390 - IAI47_20695 0.53 -1.3 388,390 - IAI47_20695 0.53 -0.7 388,396 + IAI47_20695 0.54 +0.2 388,396 + IAI47_20695 0.54 -0.2 388,397 - IAI47_20695 0.54 -1.5 388,461 + IAI47_20695 0.60 +0.5 388,462 - IAI47_20695 0.61 -0.7 388,462 - IAI47_20695 0.61 +0.5 388,462 - IAI47_20695 0.61 +1.1 388,462 - IAI47_20695 0.61 +0.1 388,462 - IAI47_20695 0.61 +0.3 388,465 + IAI47_20695 0.61 +0.2 388,465 + IAI47_20695 0.61 -0.4 388,465 + IAI47_20695 0.61 +0.5 388,465 + IAI47_20695 0.61 +1.6 388,466 - IAI47_20695 0.61 -0.6 388,466 - IAI47_20695 0.61 -1.6 388,466 - IAI47_20695 0.61 +0.9 388,466 - IAI47_20695 0.61 +0.7 388,466 - IAI47_20695 0.61 -2.7 388,523 + IAI47_20695 0.67 +0.5 388,612 + IAI47_20695 0.76 +1.1 388,687 - IAI47_20695 0.84 -0.3 388,697 - IAI47_20695 0.85 +0.2
Or see this region's nucleotide sequence