Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20125 and IAI47_20130 are separated by 44 nucleotides IAI47_20130 and IAI47_20135 are separated by 51 nucleotides IAI47_20135 and IAI47_20140 are separated by 388 nucleotides
IAI47_20125: IAI47_20125 - hypothetical protein, at 258,873 to 259,268
_20125
IAI47_20130: IAI47_20130 - nuclear transport factor 2 family protein, at 259,313 to 259,786
_20130
IAI47_20135: IAI47_20135 - 6,7-dimethyl-8-ribityllumazine synthase, at 259,838 to 260,272
_20135
IAI47_20140: IAI47_20140 - phage antirepressor KilAC domain-containing protein, at 260,661 to 261,368
_20140
Position (kb) 259
260
261 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 258.887 kb on + strand at 258.888 kb on - strand at 258.888 kb on - strand at 258.916 kb on + strand, within IAI47_20125 at 258.916 kb on + strand, within IAI47_20125 at 258.916 kb on + strand, within IAI47_20125 at 259.042 kb on + strand, within IAI47_20125 at 259.042 kb on + strand, within IAI47_20125 at 259.042 kb on + strand, within IAI47_20125 at 259.042 kb on + strand, within IAI47_20125 at 259.043 kb on - strand, within IAI47_20125 at 259.043 kb on - strand, within IAI47_20125 at 259.066 kb on + strand, within IAI47_20125 at 259.067 kb on - strand, within IAI47_20125 at 259.067 kb on - strand, within IAI47_20125 at 259.067 kb on - strand, within IAI47_20125 at 259.067 kb on - strand, within IAI47_20125 at 259.067 kb on - strand, within IAI47_20125 at 259.070 kb on + strand, within IAI47_20125 at 259.070 kb on + strand, within IAI47_20125 at 259.070 kb on + strand, within IAI47_20125 at 259.071 kb on - strand, within IAI47_20125 at 259.125 kb on + strand, within IAI47_20125 at 259.125 kb on + strand, within IAI47_20125 at 259.145 kb on + strand, within IAI47_20125 at 259.146 kb on - strand, within IAI47_20125 at 259.146 kb on - strand, within IAI47_20125 at 259.146 kb on - strand, within IAI47_20125 at 259.171 kb on + strand, within IAI47_20125 at 259.171 kb on + strand, within IAI47_20125 at 259.171 kb on + strand, within IAI47_20125 at 259.172 kb on - strand, within IAI47_20125 at 259.172 kb on - strand, within IAI47_20125 at 259.212 kb on + strand, within IAI47_20125 at 259.212 kb on + strand, within IAI47_20125 at 259.213 kb on - strand, within IAI47_20125 at 259.221 kb on - strand, within IAI47_20125 at 259.250 kb on + strand at 259.250 kb on + strand at 259.251 kb on - strand at 259.251 kb on - strand at 259.314 kb on + strand at 259.315 kb on - strand at 259.315 kb on - strand at 259.316 kb on + strand at 259.316 kb on + strand at 259.317 kb on - strand at 259.317 kb on - strand at 259.400 kb on - strand, within IAI47_20130 at 259.433 kb on + strand, within IAI47_20130 at 259.433 kb on + strand, within IAI47_20130 at 259.434 kb on - strand, within IAI47_20130 at 259.470 kb on + strand, within IAI47_20130 at 259.470 kb on + strand, within IAI47_20130 at 259.471 kb on - strand, within IAI47_20130 at 259.471 kb on - strand, within IAI47_20130 at 259.471 kb on - strand, within IAI47_20130 at 259.471 kb on - strand, within IAI47_20130 at 259.480 kb on - strand, within IAI47_20130 at 259.509 kb on + strand, within IAI47_20130 at 259.510 kb on - strand, within IAI47_20130 at 259.510 kb on - strand, within IAI47_20130 at 259.558 kb on + strand, within IAI47_20130 at 259.558 kb on + strand, within IAI47_20130 at 259.559 kb on - strand, within IAI47_20130 at 259.559 kb on - strand, within IAI47_20130 at 259.646 kb on - strand, within IAI47_20130 at 259.668 kb on + strand, within IAI47_20130 at 259.669 kb on - strand, within IAI47_20130 at 259.801 kb on - strand at 259.801 kb on - strand at 259.909 kb on + strand, within IAI47_20135 at 259.909 kb on + strand, within IAI47_20135 at 259.909 kb on + strand, within IAI47_20135 at 259.909 kb on + strand, within IAI47_20135 at 259.910 kb on - strand, within IAI47_20135 at 259.910 kb on - strand, within IAI47_20135 at 259.911 kb on + strand, within IAI47_20135 at 259.911 kb on + strand, within IAI47_20135 at 259.911 kb on + strand, within IAI47_20135 at 259.911 kb on + strand, within IAI47_20135 at 259.911 kb on + strand, within IAI47_20135 at 259.911 kb on + strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.945 kb on + strand, within IAI47_20135 at 259.946 kb on - strand, within IAI47_20135 at 260.043 kb on + strand, within IAI47_20135 at 260.043 kb on + strand, within IAI47_20135 at 260.043 kb on + strand, within IAI47_20135 at 260.043 kb on + strand, within IAI47_20135 at 260.043 kb on + strand, within IAI47_20135 at 260.043 kb on + strand, within IAI47_20135 at 260.043 kb on + strand, within IAI47_20135 at 260.044 kb on - strand, within IAI47_20135 at 260.044 kb on - strand, within IAI47_20135 at 260.044 kb on - strand, within IAI47_20135 at 260.044 kb on - strand, within IAI47_20135 at 260.057 kb on + strand, within IAI47_20135 at 260.057 kb on + strand, within IAI47_20135 at 260.057 kb on + strand, within IAI47_20135 at 260.057 kb on + strand, within IAI47_20135 at 260.058 kb on - strand, within IAI47_20135 at 260.058 kb on - strand, within IAI47_20135 at 260.058 kb on - strand, within IAI47_20135 at 260.058 kb on - strand, within IAI47_20135 at 260.120 kb on + strand, within IAI47_20135 at 260.166 kb on + strand, within IAI47_20135 at 260.166 kb on + strand, within IAI47_20135 at 260.166 kb on + strand, within IAI47_20135 at 260.167 kb on - strand, within IAI47_20135 at 260.167 kb on - strand, within IAI47_20135 at 260.245 kb on + strand at 260.245 kb on + strand at 260.246 kb on - strand at 260.248 kb on + strand at 260.248 kb on + strand at 260.249 kb on - strand at 260.249 kb on - strand at 260.286 kb on + strand at 260.286 kb on + strand at 260.286 kb on + strand at 260.286 kb on + strand at 260.287 kb on - strand at 260.288 kb on + strand at 260.288 kb on + strand at 260.289 kb on - strand at 260.289 kb on - strand at 260.289 kb on - strand at 260.384 kb on + strand at 260.384 kb on + strand at 260.384 kb on + strand at 260.384 kb on + strand at 260.384 kb on + strand at 260.384 kb on + strand at 260.384 kb on + strand at 260.385 kb on - strand at 260.385 kb on - strand at 260.385 kb on - strand at 260.385 kb on - strand at 260.386 kb on + strand at 260.386 kb on + strand at 260.386 kb on + strand at 260.386 kb on + strand at 260.392 kb on - strand at 260.392 kb on - strand at 260.397 kb on - strand at 260.423 kb on + strand at 260.423 kb on + strand at 260.423 kb on + strand at 260.424 kb on - strand at 260.424 kb on - strand at 260.425 kb on + strand at 260.429 kb on + strand at 260.429 kb on + strand at 260.429 kb on + strand at 260.429 kb on + strand at 260.429 kb on + strand at 260.430 kb on - strand at 260.430 kb on - strand at 260.430 kb on - strand at 260.457 kb on + strand at 260.457 kb on + strand at 260.457 kb on + strand at 260.457 kb on + strand at 260.457 kb on + strand at 260.457 kb on + strand at 260.457 kb on + strand at 260.458 kb on - strand at 260.458 kb on - strand at 260.458 kb on - strand at 260.458 kb on - strand at 260.458 kb on - strand at 260.458 kb on - strand at 260.458 kb on - strand at 260.509 kb on + strand at 260.509 kb on + strand at 260.510 kb on - strand at 260.531 kb on + strand at 260.531 kb on + strand at 260.532 kb on - strand at 260.548 kb on - strand at 260.562 kb on - strand at 260.602 kb on - strand at 260.613 kb on - strand at 260.613 kb on - strand at 260.668 kb on + strand at 260.669 kb on - strand at 260.810 kb on + strand, within IAI47_20140 at 260.810 kb on + strand, within IAI47_20140 at 260.810 kb on + strand, within IAI47_20140 at 260.810 kb on + strand, within IAI47_20140 at 260.810 kb on + strand, within IAI47_20140 at 260.811 kb on - strand, within IAI47_20140 at 260.811 kb on - strand, within IAI47_20140 at 260.823 kb on + strand, within IAI47_20140 at 260.824 kb on - strand, within IAI47_20140 at 260.824 kb on - strand, within IAI47_20140 at 260.824 kb on - strand, within IAI47_20140 at 260.853 kb on + strand, within IAI47_20140 at 261.074 kb on + strand, within IAI47_20140 at 261.074 kb on + strand, within IAI47_20140 at 261.180 kb on + strand, within IAI47_20140 at 261.180 kb on + strand, within IAI47_20140 at 261.180 kb on + strand, within IAI47_20140 at 261.180 kb on + strand, within IAI47_20140 at 261.180 kb on + strand, within IAI47_20140 at 261.180 kb on + strand, within IAI47_20140 at 261.181 kb on - strand, within IAI47_20140 at 261.181 kb on - strand, within IAI47_20140 at 261.181 kb on - strand, within IAI47_20140 at 261.181 kb on - strand, within IAI47_20140 at 261.181 kb on - strand, within IAI47_20140 at 261.181 kb on - strand, within IAI47_20140 at 261.181 kb on - strand, within IAI47_20140 at 261.200 kb on + strand, within IAI47_20140 at 261.201 kb on - strand, within IAI47_20140 at 261.213 kb on + strand, within IAI47_20140 at 261.213 kb on + strand, within IAI47_20140 at 261.213 kb on + strand, within IAI47_20140 at 261.214 kb on - strand, within IAI47_20140 at 261.214 kb on - strand, within IAI47_20140 at 261.214 kb on - strand, within IAI47_20140 at 261.214 kb on - strand, within IAI47_20140 at 261.231 kb on + strand, within IAI47_20140 at 261.232 kb on - strand, within IAI47_20140 at 261.236 kb on + strand, within IAI47_20140
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 258,887 + +1.7 258,888 - +1.7 258,888 - -1.2 258,916 + IAI47_20125 0.11 +0.1 258,916 + IAI47_20125 0.11 -1.7 258,916 + IAI47_20125 0.11 -2.4 259,042 + IAI47_20125 0.43 +0.1 259,042 + IAI47_20125 0.43 +2.3 259,042 + IAI47_20125 0.43 -1.0 259,042 + IAI47_20125 0.43 +0.6 259,043 - IAI47_20125 0.43 -1.7 259,043 - IAI47_20125 0.43 +1.7 259,066 + IAI47_20125 0.49 -0.5 259,067 - IAI47_20125 0.49 +2.1 259,067 - IAI47_20125 0.49 -1.3 259,067 - IAI47_20125 0.49 +0.5 259,067 - IAI47_20125 0.49 -0.0 259,067 - IAI47_20125 0.49 -0.2 259,070 + IAI47_20125 0.50 +0.1 259,070 + IAI47_20125 0.50 +1.0 259,070 + IAI47_20125 0.50 -0.4 259,071 - IAI47_20125 0.50 -2.0 259,125 + IAI47_20125 0.64 +0.5 259,125 + IAI47_20125 0.64 -0.7 259,145 + IAI47_20125 0.69 -1.3 259,146 - IAI47_20125 0.69 +1.5 259,146 - IAI47_20125 0.69 -0.4 259,146 - IAI47_20125 0.69 +0.7 259,171 + IAI47_20125 0.75 -0.8 259,171 + IAI47_20125 0.75 +1.1 259,171 + IAI47_20125 0.75 +0.5 259,172 - IAI47_20125 0.76 -0.4 259,172 - IAI47_20125 0.76 +0.6 259,212 + IAI47_20125 0.86 +0.7 259,212 + IAI47_20125 0.86 -1.3 259,213 - IAI47_20125 0.86 +1.3 259,221 - IAI47_20125 0.88 +0.2 259,250 + +0.1 259,250 + +4.9 259,251 - +0.9 259,251 - +1.4 259,314 + -1.8 259,315 - -0.1 259,315 - +0.3 259,316 + -0.4 259,316 + -0.1 259,317 - +0.7 259,317 - +0.1 259,400 - IAI47_20130 0.18 -1.9 259,433 + IAI47_20130 0.25 +0.2 259,433 + IAI47_20130 0.25 +0.4 259,434 - IAI47_20130 0.26 -0.2 259,470 + IAI47_20130 0.33 -1.9 259,470 + IAI47_20130 0.33 -0.2 259,471 - IAI47_20130 0.33 -1.6 259,471 - IAI47_20130 0.33 -2.0 259,471 - IAI47_20130 0.33 +1.6 259,471 - IAI47_20130 0.33 +2.2 259,480 - IAI47_20130 0.35 -2.2 259,509 + IAI47_20130 0.41 +0.4 259,510 - IAI47_20130 0.42 +2.7 259,510 - IAI47_20130 0.42 -1.8 259,558 + IAI47_20130 0.52 -1.3 259,558 + IAI47_20130 0.52 -1.0 259,559 - IAI47_20130 0.52 +0.7 259,559 - IAI47_20130 0.52 +0.5 259,646 - IAI47_20130 0.70 +1.1 259,668 + IAI47_20130 0.75 +1.1 259,669 - IAI47_20130 0.75 +1.0 259,801 - -0.2 259,801 - +0.3 259,909 + IAI47_20135 0.16 -1.8 259,909 + IAI47_20135 0.16 +0.8 259,909 + IAI47_20135 0.16 -0.5 259,909 + IAI47_20135 0.16 +2.5 259,910 - IAI47_20135 0.17 +1.5 259,910 - IAI47_20135 0.17 +1.2 259,911 + IAI47_20135 0.17 +1.2 259,911 + IAI47_20135 0.17 +0.9 259,911 + IAI47_20135 0.17 +0.3 259,911 + IAI47_20135 0.17 -1.3 259,911 + IAI47_20135 0.17 +1.4 259,911 + IAI47_20135 0.17 -1.4 259,912 - IAI47_20135 0.17 -0.3 259,912 - IAI47_20135 0.17 +0.9 259,912 - IAI47_20135 0.17 -1.5 259,912 - IAI47_20135 0.17 +1.1 259,912 - IAI47_20135 0.17 +0.2 259,912 - IAI47_20135 0.17 -0.0 259,912 - IAI47_20135 0.17 +0.8 259,912 - IAI47_20135 0.17 +1.9 259,912 - IAI47_20135 0.17 +0.5 259,945 + IAI47_20135 0.25 +0.5 259,946 - IAI47_20135 0.25 +1.1 260,043 + IAI47_20135 0.47 -0.0 260,043 + IAI47_20135 0.47 -0.0 260,043 + IAI47_20135 0.47 +1.0 260,043 + IAI47_20135 0.47 +1.9 260,043 + IAI47_20135 0.47 +1.0 260,043 + IAI47_20135 0.47 +0.7 260,043 + IAI47_20135 0.47 -0.0 260,044 - IAI47_20135 0.47 +1.6 260,044 - IAI47_20135 0.47 +0.3 260,044 - IAI47_20135 0.47 +0.9 260,044 - IAI47_20135 0.47 +0.4 260,057 + IAI47_20135 0.50 +1.0 260,057 + IAI47_20135 0.50 -2.1 260,057 + IAI47_20135 0.50 +1.3 260,057 + IAI47_20135 0.50 +1.2 260,058 - IAI47_20135 0.51 +0.9 260,058 - IAI47_20135 0.51 -1.0 260,058 - IAI47_20135 0.51 +2.0 260,058 - IAI47_20135 0.51 +3.0 260,120 + IAI47_20135 0.65 +1.7 260,166 + IAI47_20135 0.75 -1.7 260,166 + IAI47_20135 0.75 -0.1 260,166 + IAI47_20135 0.75 -0.9 260,167 - IAI47_20135 0.76 +1.9 260,167 - IAI47_20135 0.76 -1.2 260,245 + +1.8 260,245 + +0.2 260,246 - +0.6 260,248 + -1.2 260,248 + -0.3 260,249 - +0.8 260,249 - +2.2 260,286 + -0.3 260,286 + -0.5 260,286 + +1.6 260,286 + +0.2 260,287 - -0.2 260,288 + +2.2 260,288 + +0.5 260,289 - +0.9 260,289 - -0.7 260,289 - -1.5 260,384 + +0.3 260,384 + -0.0 260,384 + -0.0 260,384 + +1.4 260,384 + -1.8 260,384 + +1.6 260,384 + +1.4 260,385 - +0.8 260,385 - +0.8 260,385 - -0.4 260,385 - -1.5 260,386 + +0.2 260,386 + +1.7 260,386 + +0.7 260,386 + -0.3 260,392 - -0.7 260,392 - +0.5 260,397 - +2.1 260,423 + +1.9 260,423 + -2.5 260,423 + -1.5 260,424 - -1.3 260,424 - +0.4 260,425 + -0.3 260,429 + +0.7 260,429 + -1.0 260,429 + +0.6 260,429 + +0.8 260,429 + +1.2 260,430 - +1.8 260,430 - +1.1 260,430 - -1.9 260,457 + +0.6 260,457 + -0.7 260,457 + +1.7 260,457 + -0.3 260,457 + +0.5 260,457 + +2.3 260,457 + +0.4 260,458 - -0.2 260,458 - -0.5 260,458 - +0.1 260,458 - -1.9 260,458 - -1.0 260,458 - -1.1 260,458 - -0.6 260,509 + +1.2 260,509 + -1.2 260,510 - -1.1 260,531 + +0.6 260,531 + +1.9 260,532 - -0.9 260,548 - -0.0 260,562 - -1.3 260,602 - +0.2 260,613 - -0.1 260,613 - +1.1 260,668 + +1.1 260,669 - +0.8 260,810 + IAI47_20140 0.21 -1.5 260,810 + IAI47_20140 0.21 +0.6 260,810 + IAI47_20140 0.21 -0.7 260,810 + IAI47_20140 0.21 +2.5 260,810 + IAI47_20140 0.21 -0.3 260,811 - IAI47_20140 0.21 +0.3 260,811 - IAI47_20140 0.21 -1.5 260,823 + IAI47_20140 0.23 +1.3 260,824 - IAI47_20140 0.23 -0.8 260,824 - IAI47_20140 0.23 +0.0 260,824 - IAI47_20140 0.23 +0.4 260,853 + IAI47_20140 0.27 -2.0 261,074 + IAI47_20140 0.58 +0.3 261,074 + IAI47_20140 0.58 -0.4 261,180 + IAI47_20140 0.73 +1.5 261,180 + IAI47_20140 0.73 +0.9 261,180 + IAI47_20140 0.73 +0.3 261,180 + IAI47_20140 0.73 +0.2 261,180 + IAI47_20140 0.73 +0.6 261,180 + IAI47_20140 0.73 -0.9 261,181 - IAI47_20140 0.73 -1.4 261,181 - IAI47_20140 0.73 -2.2 261,181 - IAI47_20140 0.73 +1.2 261,181 - IAI47_20140 0.73 +2.2 261,181 - IAI47_20140 0.73 +1.3 261,181 - IAI47_20140 0.73 -1.8 261,181 - IAI47_20140 0.73 -0.2 261,200 + IAI47_20140 0.76 +2.3 261,201 - IAI47_20140 0.76 +0.9 261,213 + IAI47_20140 0.78 -1.4 261,213 + IAI47_20140 0.78 +1.3 261,213 + IAI47_20140 0.78 +1.4 261,214 - IAI47_20140 0.78 -2.8 261,214 - IAI47_20140 0.78 +0.7 261,214 - IAI47_20140 0.78 +2.1 261,214 - IAI47_20140 0.78 -0.8 261,231 + IAI47_20140 0.81 +0.5 261,232 - IAI47_20140 0.81 -1.8 261,236 + IAI47_20140 0.81 -1.8
Or see this region's nucleotide sequence