Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01650 and IAI47_01655 are separated by 140 nucleotides IAI47_01655 and IAI47_01660 are separated by 2 nucleotides IAI47_01660 and IAI47_01665 are separated by 24 nucleotides IAI47_01665 and IAI47_01670 are separated by 333 nucleotides
IAI47_01650: IAI47_01650 - gluconate operon transcriptional repressor GntR, at 358,379 to 359,374
_01650
IAI47_01655: IAI47_01655 - gluconokinase, at 359,515 to 360,051
_01655
IAI47_01660: IAI47_01660 - gluconate transporter, at 360,054 to 361,394
_01660
IAI47_01665: IAI47_01665 - DUF805 domain-containing protein, at 361,419 to 361,742
_01665
IAI47_01670: IAI47_01670 - YhgN family NAAT transporter, at 362,076 to 362,669
_01670
Position (kb) 360
361
362 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 359.088 kb on - strand, within IAI47_01650 at 359.088 kb on - strand, within IAI47_01650 at 359.113 kb on + strand, within IAI47_01650 at 359.113 kb on + strand, within IAI47_01650 at 359.114 kb on - strand, within IAI47_01650 at 359.114 kb on - strand, within IAI47_01650 at 359.166 kb on + strand, within IAI47_01650 at 359.166 kb on + strand, within IAI47_01650 at 359.166 kb on + strand, within IAI47_01650 at 359.166 kb on + strand, within IAI47_01650 at 359.167 kb on - strand, within IAI47_01650 at 359.167 kb on - strand, within IAI47_01650 at 359.167 kb on - strand, within IAI47_01650 at 359.167 kb on - strand, within IAI47_01650 at 359.167 kb on - strand, within IAI47_01650 at 359.293 kb on - strand at 359.323 kb on + strand at 359.324 kb on - strand at 359.387 kb on + strand at 359.388 kb on - strand at 359.711 kb on + strand, within IAI47_01655 at 359.763 kb on + strand, within IAI47_01655 at 359.763 kb on + strand, within IAI47_01655 at 359.764 kb on - strand, within IAI47_01655 at 359.764 kb on - strand, within IAI47_01655 at 359.764 kb on - strand, within IAI47_01655 at 359.764 kb on - strand, within IAI47_01655 at 359.764 kb on - strand, within IAI47_01655 at 359.764 kb on - strand, within IAI47_01655 at 359.764 kb on - strand, within IAI47_01655 at 359.764 kb on - strand, within IAI47_01655 at 359.794 kb on + strand, within IAI47_01655 at 359.839 kb on + strand, within IAI47_01655 at 359.839 kb on + strand, within IAI47_01655 at 359.840 kb on - strand, within IAI47_01655 at 359.863 kb on - strand, within IAI47_01655 at 359.863 kb on - strand, within IAI47_01655 at 359.886 kb on + strand, within IAI47_01655 at 359.887 kb on - strand, within IAI47_01655 at 359.887 kb on - strand, within IAI47_01655 at 359.919 kb on - strand, within IAI47_01655 at 359.966 kb on + strand, within IAI47_01655 at 359.966 kb on + strand, within IAI47_01655 at 359.966 kb on + strand, within IAI47_01655 at 359.967 kb on - strand, within IAI47_01655 at 359.967 kb on - strand, within IAI47_01655 at 359.967 kb on - strand, within IAI47_01655 at 359.967 kb on - strand, within IAI47_01655 at 359.967 kb on - strand, within IAI47_01655 at 360.012 kb on + strand at 360.012 kb on + strand at 360.012 kb on + strand at 360.013 kb on - strand at 360.013 kb on - strand at 360.036 kb on + strand at 360.036 kb on + strand at 360.037 kb on - strand at 360.037 kb on - strand at 360.050 kb on - strand at 360.050 kb on - strand at 360.059 kb on + strand at 360.060 kb on - strand at 360.154 kb on + strand at 360.154 kb on + strand at 360.154 kb on + strand at 360.154 kb on + strand at 360.155 kb on - strand at 360.155 kb on - strand at 360.155 kb on - strand at 360.155 kb on - strand at 360.155 kb on - strand at 360.155 kb on - strand at 360.320 kb on + strand, within IAI47_01660 at 360.320 kb on + strand, within IAI47_01660 at 360.321 kb on - strand, within IAI47_01660 at 360.321 kb on - strand, within IAI47_01660 at 360.360 kb on + strand, within IAI47_01660 at 360.360 kb on + strand, within IAI47_01660 at 360.360 kb on + strand, within IAI47_01660 at 360.361 kb on - strand, within IAI47_01660 at 360.361 kb on - strand, within IAI47_01660 at 360.822 kb on + strand, within IAI47_01660 at 360.823 kb on - strand, within IAI47_01660 at 360.823 kb on - strand, within IAI47_01660 at 360.823 kb on - strand, within IAI47_01660 at 360.823 kb on - strand, within IAI47_01660 at 360.823 kb on - strand, within IAI47_01660 at 360.823 kb on - strand, within IAI47_01660 at 360.888 kb on + strand, within IAI47_01660 at 360.888 kb on + strand, within IAI47_01660 at 360.889 kb on - strand, within IAI47_01660 at 360.889 kb on - strand, within IAI47_01660 at 360.900 kb on + strand, within IAI47_01660 at 360.901 kb on - strand, within IAI47_01660 at 360.917 kb on + strand, within IAI47_01660 at 360.968 kb on + strand, within IAI47_01660 at 360.968 kb on + strand, within IAI47_01660 at 361.009 kb on + strand, within IAI47_01660 at 361.009 kb on + strand, within IAI47_01660 at 361.010 kb on - strand, within IAI47_01660 at 361.088 kb on + strand, within IAI47_01660 at 361.088 kb on + strand, within IAI47_01660 at 361.088 kb on + strand, within IAI47_01660 at 361.088 kb on + strand, within IAI47_01660 at 361.156 kb on + strand, within IAI47_01660 at 361.157 kb on - strand, within IAI47_01660 at 361.157 kb on - strand, within IAI47_01660 at 361.185 kb on + strand, within IAI47_01660 at 361.185 kb on + strand, within IAI47_01660 at 361.185 kb on + strand, within IAI47_01660 at 361.185 kb on + strand, within IAI47_01660 at 361.186 kb on - strand, within IAI47_01660 at 361.241 kb on + strand, within IAI47_01660 at 361.241 kb on + strand, within IAI47_01660 at 361.242 kb on - strand, within IAI47_01660 at 361.410 kb on - strand at 361.468 kb on + strand, within IAI47_01665 at 361.468 kb on + strand, within IAI47_01665 at 361.469 kb on - strand, within IAI47_01665 at 361.521 kb on + strand, within IAI47_01665 at 361.522 kb on - strand, within IAI47_01665 at 361.522 kb on - strand, within IAI47_01665 at 361.522 kb on - strand, within IAI47_01665 at 361.557 kb on - strand, within IAI47_01665 at 361.557 kb on - strand, within IAI47_01665 at 361.615 kb on + strand, within IAI47_01665 at 361.615 kb on + strand, within IAI47_01665 at 361.616 kb on - strand, within IAI47_01665 at 361.616 kb on - strand, within IAI47_01665 at 361.652 kb on + strand, within IAI47_01665 at 361.652 kb on + strand, within IAI47_01665 at 361.658 kb on - strand, within IAI47_01665 at 361.658 kb on - strand, within IAI47_01665 at 361.658 kb on - strand, within IAI47_01665 at 361.684 kb on + strand, within IAI47_01665 at 361.685 kb on - strand, within IAI47_01665 at 361.688 kb on + strand, within IAI47_01665 at 361.688 kb on + strand, within IAI47_01665 at 361.688 kb on + strand, within IAI47_01665 at 361.688 kb on + strand, within IAI47_01665 at 361.688 kb on + strand, within IAI47_01665 at 361.688 kb on + strand, within IAI47_01665 at 361.689 kb on - strand, within IAI47_01665 at 361.689 kb on - strand, within IAI47_01665 at 361.689 kb on - strand, within IAI47_01665 at 361.689 kb on - strand, within IAI47_01665 at 361.689 kb on - strand, within IAI47_01665 at 361.689 kb on - strand, within IAI47_01665 at 361.689 kb on - strand, within IAI47_01665 at 361.689 kb on - strand, within IAI47_01665 at 361.689 kb on - strand, within IAI47_01665 at 361.751 kb on + strand at 361.752 kb on - strand at 361.791 kb on + strand at 361.791 kb on + strand at 361.791 kb on + strand at 361.792 kb on - strand at 361.792 kb on - strand at 361.792 kb on - strand at 361.795 kb on + strand at 361.795 kb on + strand at 361.795 kb on + strand at 361.795 kb on + strand at 361.796 kb on - strand at 361.796 kb on - strand at 361.796 kb on - strand at 361.796 kb on - strand at 361.796 kb on - strand at 361.814 kb on + strand at 361.872 kb on + strand at 361.872 kb on + strand at 361.873 kb on - strand at 361.873 kb on - strand at 361.873 kb on - strand at 361.875 kb on + strand at 361.875 kb on + strand at 361.875 kb on + strand at 361.875 kb on + strand at 361.876 kb on - strand at 361.876 kb on - strand at 361.876 kb on - strand at 361.876 kb on - strand at 361.876 kb on - strand at 361.876 kb on - strand at 361.876 kb on - strand at 361.876 kb on - strand at 361.877 kb on - strand at 361.928 kb on + strand at 361.938 kb on + strand at 361.954 kb on + strand at 361.954 kb on + strand at 361.954 kb on + strand at 361.954 kb on + strand at 361.955 kb on - strand at 361.955 kb on - strand at 361.955 kb on - strand at 361.955 kb on - strand at 361.972 kb on + strand at 361.972 kb on + strand at 361.972 kb on + strand at 361.972 kb on + strand at 361.972 kb on + strand at 361.972 kb on + strand at 361.972 kb on + strand at 361.972 kb on + strand at 361.973 kb on - strand at 361.973 kb on - strand at 361.973 kb on - strand at 361.974 kb on + strand at 361.974 kb on + strand at 361.974 kb on + strand at 361.975 kb on - strand at 361.975 kb on - strand at 361.975 kb on - strand at 361.975 kb on - strand at 361.975 kb on - strand at 361.975 kb on - strand at 361.976 kb on + strand at 361.976 kb on + strand at 361.977 kb on - strand at 361.977 kb on - strand at 361.977 kb on - strand at 361.977 kb on - strand at 361.977 kb on - strand at 361.980 kb on + strand at 361.980 kb on + strand at 361.980 kb on + strand at 361.981 kb on - strand at 361.983 kb on + strand at 361.984 kb on - strand at 361.987 kb on - strand at 362.014 kb on + strand at 362.014 kb on + strand at 362.014 kb on + strand at 362.014 kb on + strand at 362.058 kb on - strand at 362.058 kb on - strand at 362.058 kb on - strand at 362.077 kb on + strand at 362.077 kb on + strand at 362.077 kb on + strand at 362.077 kb on + strand at 362.077 kb on + strand at 362.078 kb on - strand at 362.078 kb on - strand at 362.078 kb on - strand at 362.078 kb on - strand at 362.089 kb on + strand at 362.129 kb on + strand at 362.129 kb on + strand at 362.129 kb on + strand at 362.129 kb on + strand at 362.130 kb on - strand at 362.130 kb on - strand at 362.130 kb on - strand at 362.130 kb on - strand at 362.130 kb on - strand at 362.136 kb on + strand, within IAI47_01670 at 362.136 kb on + strand, within IAI47_01670 at 362.137 kb on - strand, within IAI47_01670 at 362.137 kb on - strand, within IAI47_01670 at 362.175 kb on - strand, within IAI47_01670 at 362.175 kb on - strand, within IAI47_01670 at 362.175 kb on - strand, within IAI47_01670 at 362.175 kb on - strand, within IAI47_01670 at 362.175 kb on - strand, within IAI47_01670 at 362.278 kb on + strand, within IAI47_01670 at 362.279 kb on - strand, within IAI47_01670 at 362.279 kb on - strand, within IAI47_01670 at 362.374 kb on - strand, within IAI47_01670
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 359,088 - IAI47_01650 0.71 -3.0 359,088 - IAI47_01650 0.71 -2.6 359,113 + IAI47_01650 0.74 -1.3 359,113 + IAI47_01650 0.74 -0.7 359,114 - IAI47_01650 0.74 -2.2 359,114 - IAI47_01650 0.74 -1.8 359,166 + IAI47_01650 0.79 -2.0 359,166 + IAI47_01650 0.79 -3.0 359,166 + IAI47_01650 0.79 -1.6 359,166 + IAI47_01650 0.79 -1.6 359,167 - IAI47_01650 0.79 -0.6 359,167 - IAI47_01650 0.79 -1.9 359,167 - IAI47_01650 0.79 -1.5 359,167 - IAI47_01650 0.79 -2.0 359,167 - IAI47_01650 0.79 -3.9 359,293 - -2.3 359,323 + -0.7 359,324 - -0.9 359,387 + -1.5 359,388 - +0.9 359,711 + IAI47_01655 0.36 -0.1 359,763 + IAI47_01655 0.46 +1.5 359,763 + IAI47_01655 0.46 -1.2 359,764 - IAI47_01655 0.46 -1.0 359,764 - IAI47_01655 0.46 -0.4 359,764 - IAI47_01655 0.46 -0.2 359,764 - IAI47_01655 0.46 +1.0 359,764 - IAI47_01655 0.46 +0.4 359,764 - IAI47_01655 0.46 +0.8 359,764 - IAI47_01655 0.46 -1.3 359,764 - IAI47_01655 0.46 +0.3 359,794 + IAI47_01655 0.52 -1.3 359,839 + IAI47_01655 0.60 +0.4 359,839 + IAI47_01655 0.60 -3.2 359,840 - IAI47_01655 0.61 -1.1 359,863 - IAI47_01655 0.65 -0.9 359,863 - IAI47_01655 0.65 -0.7 359,886 + IAI47_01655 0.69 +0.2 359,887 - IAI47_01655 0.69 -1.4 359,887 - IAI47_01655 0.69 -0.0 359,919 - IAI47_01655 0.75 -0.3 359,966 + IAI47_01655 0.84 +2.9 359,966 + IAI47_01655 0.84 -0.9 359,966 + IAI47_01655 0.84 -0.3 359,967 - IAI47_01655 0.84 +1.3 359,967 - IAI47_01655 0.84 -0.5 359,967 - IAI47_01655 0.84 +0.2 359,967 - IAI47_01655 0.84 -0.2 359,967 - IAI47_01655 0.84 +0.7 360,012 + -0.6 360,012 + +0.7 360,012 + -0.0 360,013 - +0.8 360,013 - -1.2 360,036 + -0.2 360,036 + +1.0 360,037 - +1.2 360,037 - +1.6 360,050 - -2.4 360,050 - +2.2 360,059 + +0.1 360,060 - -0.3 360,154 + +0.7 360,154 + +1.1 360,154 + +2.1 360,154 + +2.9 360,155 - -1.4 360,155 - +0.9 360,155 - -1.0 360,155 - +1.0 360,155 - -0.0 360,155 - -0.9 360,320 + IAI47_01660 0.20 -1.5 360,320 + IAI47_01660 0.20 +0.8 360,321 - IAI47_01660 0.20 +1.7 360,321 - IAI47_01660 0.20 +0.8 360,360 + IAI47_01660 0.23 -0.1 360,360 + IAI47_01660 0.23 +2.0 360,360 + IAI47_01660 0.23 +1.1 360,361 - IAI47_01660 0.23 -0.1 360,361 - IAI47_01660 0.23 -2.3 360,822 + IAI47_01660 0.57 +0.5 360,823 - IAI47_01660 0.57 +0.1 360,823 - IAI47_01660 0.57 -0.2 360,823 - IAI47_01660 0.57 -0.8 360,823 - IAI47_01660 0.57 +0.0 360,823 - IAI47_01660 0.57 +1.5 360,823 - IAI47_01660 0.57 +2.4 360,888 + IAI47_01660 0.62 +1.5 360,888 + IAI47_01660 0.62 +1.9 360,889 - IAI47_01660 0.62 +0.6 360,889 - IAI47_01660 0.62 +0.2 360,900 + IAI47_01660 0.63 -0.8 360,901 - IAI47_01660 0.63 +1.1 360,917 + IAI47_01660 0.64 -0.9 360,968 + IAI47_01660 0.68 +0.9 360,968 + IAI47_01660 0.68 +0.8 361,009 + IAI47_01660 0.71 -1.6 361,009 + IAI47_01660 0.71 +1.7 361,010 - IAI47_01660 0.71 -1.9 361,088 + IAI47_01660 0.77 +1.1 361,088 + IAI47_01660 0.77 +0.5 361,088 + IAI47_01660 0.77 +0.7 361,088 + IAI47_01660 0.77 +1.2 361,156 + IAI47_01660 0.82 -1.0 361,157 - IAI47_01660 0.82 +1.7 361,157 - IAI47_01660 0.82 +1.8 361,185 + IAI47_01660 0.84 +0.7 361,185 + IAI47_01660 0.84 +0.3 361,185 + IAI47_01660 0.84 +1.5 361,185 + IAI47_01660 0.84 +2.0 361,186 - IAI47_01660 0.84 -0.5 361,241 + IAI47_01660 0.89 -2.0 361,241 + IAI47_01660 0.89 +2.1 361,242 - IAI47_01660 0.89 +1.0 361,410 - +1.2 361,468 + IAI47_01665 0.15 -2.8 361,468 + IAI47_01665 0.15 +1.1 361,469 - IAI47_01665 0.15 +0.8 361,521 + IAI47_01665 0.31 -1.1 361,522 - IAI47_01665 0.32 -0.8 361,522 - IAI47_01665 0.32 -2.0 361,522 - IAI47_01665 0.32 +1.6 361,557 - IAI47_01665 0.43 +1.3 361,557 - IAI47_01665 0.43 +1.2 361,615 + IAI47_01665 0.60 +0.2 361,615 + IAI47_01665 0.60 -1.9 361,616 - IAI47_01665 0.61 +0.2 361,616 - IAI47_01665 0.61 -0.3 361,652 + IAI47_01665 0.72 +0.2 361,652 + IAI47_01665 0.72 +0.2 361,658 - IAI47_01665 0.74 +2.7 361,658 - IAI47_01665 0.74 +1.0 361,658 - IAI47_01665 0.74 +0.0 361,684 + IAI47_01665 0.82 -0.4 361,685 - IAI47_01665 0.82 -1.6 361,688 + IAI47_01665 0.83 +0.4 361,688 + IAI47_01665 0.83 +0.4 361,688 + IAI47_01665 0.83 +0.2 361,688 + IAI47_01665 0.83 +0.4 361,688 + IAI47_01665 0.83 -1.3 361,688 + IAI47_01665 0.83 -0.5 361,689 - IAI47_01665 0.83 +1.5 361,689 - IAI47_01665 0.83 -0.3 361,689 - IAI47_01665 0.83 +0.2 361,689 - IAI47_01665 0.83 -0.9 361,689 - IAI47_01665 0.83 +0.2 361,689 - IAI47_01665 0.83 +1.0 361,689 - IAI47_01665 0.83 -1.6 361,689 - IAI47_01665 0.83 -2.6 361,689 - IAI47_01665 0.83 -0.4 361,751 + -0.4 361,752 - -0.9 361,791 + -1.1 361,791 + -0.5 361,791 + +0.3 361,792 - +0.2 361,792 - -1.1 361,792 - -1.2 361,795 + -1.8 361,795 + -1.5 361,795 + +0.6 361,795 + -0.6 361,796 - -0.1 361,796 - -0.4 361,796 - +0.8 361,796 - -0.5 361,796 - +1.0 361,814 + +0.6 361,872 + -0.5 361,872 + +0.9 361,873 - -0.7 361,873 - +1.3 361,873 - -0.8 361,875 + -1.3 361,875 + +1.7 361,875 + +2.3 361,875 + +0.0 361,876 - +0.4 361,876 - -2.4 361,876 - -0.0 361,876 - -1.5 361,876 - -0.5 361,876 - -2.3 361,876 - -0.3 361,876 - +1.9 361,877 - -2.1 361,928 + -0.3 361,938 + +2.3 361,954 + -2.3 361,954 + +0.6 361,954 + -0.4 361,954 + -0.2 361,955 - +1.4 361,955 - +0.2 361,955 - -0.1 361,955 - +1.3 361,972 + +0.8 361,972 + -0.1 361,972 + -2.1 361,972 + -1.1 361,972 + +0.9 361,972 + +0.1 361,972 + +0.4 361,972 + +1.2 361,973 - +0.8 361,973 - +0.4 361,973 - +1.6 361,974 + -0.3 361,974 + -1.6 361,974 + +1.0 361,975 - +0.5 361,975 - +0.1 361,975 - -0.5 361,975 - +1.1 361,975 - -0.4 361,975 - +2.0 361,976 + +0.6 361,976 + -2.8 361,977 - +1.5 361,977 - +1.2 361,977 - -0.9 361,977 - +0.6 361,977 - -0.9 361,980 + +0.8 361,980 + -1.4 361,980 + -0.2 361,981 - +0.0 361,983 + +2.0 361,984 - -0.3 361,987 - -0.5 362,014 + +1.8 362,014 + +0.1 362,014 + -2.7 362,014 + +0.6 362,058 - +0.5 362,058 - -0.2 362,058 - -1.1 362,077 + -0.2 362,077 + -0.4 362,077 + -2.6 362,077 + +0.5 362,077 + +0.2 362,078 - +0.3 362,078 - -3.2 362,078 - -2.5 362,078 - +1.3 362,089 + +0.5 362,129 + +1.3 362,129 + -1.1 362,129 + +0.8 362,129 + +0.5 362,130 - -0.1 362,130 - -1.8 362,130 - -0.7 362,130 - +0.4 362,130 - +0.7 362,136 + IAI47_01670 0.10 -1.3 362,136 + IAI47_01670 0.10 -1.2 362,137 - IAI47_01670 0.10 -0.0 362,137 - IAI47_01670 0.10 -1.0 362,175 - IAI47_01670 0.17 -0.7 362,175 - IAI47_01670 0.17 +1.1 362,175 - IAI47_01670 0.17 -0.4 362,175 - IAI47_01670 0.17 +0.7 362,175 - IAI47_01670 0.17 -0.3 362,278 + IAI47_01670 0.34 +0.2 362,279 - IAI47_01670 0.34 -0.1 362,279 - IAI47_01670 0.34 -0.9 362,374 - IAI47_01670 0.50 -0.3
Or see this region's nucleotide sequence