Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20775 and IAI47_20780 are separated by 89 nucleotides IAI47_20780 and IAI47_20785 are separated by 60 nucleotides IAI47_20785 and IAI47_20790 are separated by 158 nucleotides
IAI47_20775: IAI47_20775 - hydroxyacid dehydrogenase, at 400,189 to 401,139
_20775
IAI47_20780: IAI47_20780 - cupin domain-containing protein, at 401,229 to 401,576
_20780
IAI47_20785: IAI47_20785 - GNAT family N-acetyltransferase, at 401,637 to 402,125
_20785
IAI47_20790: IAI47_20790 - dienelactone hydrolase, at 402,284 to 403,453
_20790
Position (kb) 401
402 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 400.276 kb on + strand at 400.276 kb on + strand at 400.276 kb on + strand at 400.276 kb on + strand at 400.307 kb on + strand, within IAI47_20775 at 400.307 kb on + strand, within IAI47_20775 at 400.359 kb on + strand, within IAI47_20775 at 400.360 kb on - strand, within IAI47_20775 at 400.360 kb on - strand, within IAI47_20775 at 400.434 kb on + strand, within IAI47_20775 at 400.435 kb on - strand, within IAI47_20775 at 400.435 kb on - strand, within IAI47_20775 at 400.435 kb on - strand, within IAI47_20775 at 400.435 kb on - strand, within IAI47_20775 at 400.465 kb on - strand, within IAI47_20775 at 400.515 kb on + strand, within IAI47_20775 at 400.516 kb on - strand, within IAI47_20775 at 400.553 kb on + strand, within IAI47_20775 at 400.554 kb on - strand, within IAI47_20775 at 400.554 kb on - strand, within IAI47_20775 at 400.569 kb on + strand, within IAI47_20775 at 400.569 kb on + strand, within IAI47_20775 at 400.569 kb on + strand, within IAI47_20775 at 400.582 kb on + strand, within IAI47_20775 at 400.590 kb on + strand, within IAI47_20775 at 400.590 kb on + strand, within IAI47_20775 at 400.590 kb on + strand, within IAI47_20775 at 400.591 kb on - strand, within IAI47_20775 at 400.602 kb on + strand, within IAI47_20775 at 400.602 kb on + strand, within IAI47_20775 at 400.603 kb on - strand, within IAI47_20775 at 400.603 kb on - strand, within IAI47_20775 at 400.603 kb on - strand, within IAI47_20775 at 400.708 kb on + strand, within IAI47_20775 at 400.708 kb on + strand, within IAI47_20775 at 400.709 kb on - strand, within IAI47_20775 at 400.855 kb on - strand, within IAI47_20775 at 400.992 kb on + strand, within IAI47_20775 at 400.992 kb on + strand, within IAI47_20775 at 400.992 kb on + strand, within IAI47_20775 at 400.993 kb on - strand, within IAI47_20775 at 400.993 kb on - strand, within IAI47_20775 at 400.993 kb on - strand, within IAI47_20775 at 400.993 kb on - strand, within IAI47_20775 at 400.993 kb on - strand, within IAI47_20775 at 400.993 kb on - strand, within IAI47_20775 at 401.015 kb on + strand, within IAI47_20775 at 401.015 kb on + strand, within IAI47_20775 at 401.015 kb on + strand, within IAI47_20775 at 401.015 kb on + strand, within IAI47_20775 at 401.015 kb on + strand, within IAI47_20775 at 401.016 kb on - strand, within IAI47_20775 at 401.016 kb on - strand, within IAI47_20775 at 401.049 kb on + strand at 401.050 kb on - strand at 401.050 kb on - strand at 401.050 kb on - strand at 401.056 kb on + strand at 401.056 kb on + strand at 401.057 kb on - strand at 401.057 kb on - strand at 401.057 kb on - strand at 401.057 kb on - strand at 401.085 kb on + strand at 401.085 kb on + strand at 401.085 kb on + strand at 401.085 kb on + strand at 401.086 kb on - strand at 401.086 kb on - strand at 401.115 kb on + strand at 401.116 kb on - strand at 401.137 kb on + strand at 401.138 kb on - strand at 401.157 kb on + strand at 401.158 kb on - strand at 401.180 kb on + strand at 401.180 kb on + strand at 401.180 kb on + strand at 401.180 kb on + strand at 401.180 kb on + strand at 401.181 kb on - strand at 401.181 kb on - strand at 401.203 kb on + strand at 401.204 kb on - strand at 401.204 kb on - strand at 401.204 kb on - strand at 401.204 kb on - strand at 401.204 kb on - strand at 401.204 kb on - strand at 401.213 kb on + strand at 401.214 kb on - strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.256 kb on + strand at 401.257 kb on - strand at 401.257 kb on - strand at 401.257 kb on - strand at 401.257 kb on - strand at 401.257 kb on - strand at 401.257 kb on - strand at 401.257 kb on - strand at 401.257 kb on - strand at 401.257 kb on - strand at 401.260 kb on + strand at 401.260 kb on + strand at 401.260 kb on + strand at 401.260 kb on + strand at 401.260 kb on + strand at 401.260 kb on + strand at 401.260 kb on + strand at 401.260 kb on + strand at 401.260 kb on + strand at 401.260 kb on + strand at 401.260 kb on + strand at 401.260 kb on + strand at 401.260 kb on + strand at 401.261 kb on - strand at 401.261 kb on - strand at 401.261 kb on - strand at 401.261 kb on - strand at 401.261 kb on - strand at 401.261 kb on - strand at 401.261 kb on - strand at 401.286 kb on + strand, within IAI47_20780 at 401.389 kb on + strand, within IAI47_20780 at 401.389 kb on + strand, within IAI47_20780 at 401.389 kb on + strand, within IAI47_20780 at 401.389 kb on + strand, within IAI47_20780 at 401.390 kb on - strand, within IAI47_20780 at 401.390 kb on - strand, within IAI47_20780 at 401.390 kb on - strand, within IAI47_20780 at 401.405 kb on + strand, within IAI47_20780 at 401.405 kb on + strand, within IAI47_20780 at 401.405 kb on + strand, within IAI47_20780 at 401.405 kb on + strand, within IAI47_20780 at 401.406 kb on - strand, within IAI47_20780 at 401.406 kb on - strand, within IAI47_20780 at 401.406 kb on - strand, within IAI47_20780 at 401.406 kb on - strand, within IAI47_20780 at 401.602 kb on + strand at 401.602 kb on + strand at 401.602 kb on + strand at 401.603 kb on - strand at 401.648 kb on + strand at 401.683 kb on + strand at 401.683 kb on + strand at 401.683 kb on + strand at 401.684 kb on - strand at 401.684 kb on - strand at 401.684 kb on - strand at 401.789 kb on + strand, within IAI47_20785 at 401.790 kb on - strand, within IAI47_20785 at 401.790 kb on - strand, within IAI47_20785 at 401.807 kb on + strand, within IAI47_20785 at 401.808 kb on - strand, within IAI47_20785 at 401.808 kb on - strand, within IAI47_20785 at 401.825 kb on + strand, within IAI47_20785 at 401.826 kb on - strand, within IAI47_20785 at 401.826 kb on - strand, within IAI47_20785 at 401.826 kb on - strand, within IAI47_20785 at 401.888 kb on + strand, within IAI47_20785 at 401.888 kb on + strand, within IAI47_20785 at 401.889 kb on - strand, within IAI47_20785 at 401.890 kb on + strand, within IAI47_20785 at 401.906 kb on - strand, within IAI47_20785 at 401.913 kb on - strand, within IAI47_20785 at 401.913 kb on - strand, within IAI47_20785 at 401.997 kb on - strand, within IAI47_20785 at 401.997 kb on - strand, within IAI47_20785 at 402.004 kb on + strand, within IAI47_20785 at 402.005 kb on - strand, within IAI47_20785 at 402.029 kb on + strand, within IAI47_20785 at 402.030 kb on - strand, within IAI47_20785 at 402.039 kb on - strand, within IAI47_20785 at 402.043 kb on + strand, within IAI47_20785 at 402.046 kb on + strand, within IAI47_20785 at 402.046 kb on + strand, within IAI47_20785 at 402.046 kb on + strand, within IAI47_20785 at 402.046 kb on + strand, within IAI47_20785 at 402.046 kb on + strand, within IAI47_20785 at 402.047 kb on - strand, within IAI47_20785 at 402.047 kb on - strand, within IAI47_20785 at 402.049 kb on + strand, within IAI47_20785 at 402.049 kb on + strand, within IAI47_20785 at 402.050 kb on - strand, within IAI47_20785 at 402.050 kb on - strand, within IAI47_20785 at 402.050 kb on - strand, within IAI47_20785 at 402.050 kb on - strand, within IAI47_20785 at 402.050 kb on - strand, within IAI47_20785 at 402.050 kb on - strand, within IAI47_20785 at 402.117 kb on + strand at 402.117 kb on + strand at 402.118 kb on - strand at 402.129 kb on + strand at 402.129 kb on + strand at 402.145 kb on - strand at 402.147 kb on + strand at 402.148 kb on - strand at 402.148 kb on - strand at 402.148 kb on - strand at 402.176 kb on + strand at 402.177 kb on - strand at 402.179 kb on + strand at 402.179 kb on + strand at 402.180 kb on - strand at 402.180 kb on - strand at 402.180 kb on - strand at 402.186 kb on + strand at 402.186 kb on + strand at 402.209 kb on + strand at 402.210 kb on - strand at 402.218 kb on + strand at 402.228 kb on + strand at 402.229 kb on - strand at 402.229 kb on - strand at 402.264 kb on + strand at 402.277 kb on - strand at 402.282 kb on + strand at 402.301 kb on + strand at 402.301 kb on + strand at 402.302 kb on - strand at 402.302 kb on - strand at 402.312 kb on + strand at 402.313 kb on - strand at 402.313 kb on - strand at 402.361 kb on + strand at 402.361 kb on + strand at 402.362 kb on - strand at 402.362 kb on - strand at 402.403 kb on - strand, within IAI47_20790 at 402.403 kb on - strand, within IAI47_20790 at 402.451 kb on - strand, within IAI47_20790 at 402.468 kb on + strand, within IAI47_20790 at 402.468 kb on + strand, within IAI47_20790 at 402.482 kb on + strand, within IAI47_20790 at 402.482 kb on + strand, within IAI47_20790 at 402.483 kb on - strand, within IAI47_20790 at 402.555 kb on + strand, within IAI47_20790 at 402.555 kb on + strand, within IAI47_20790
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 400,276 + +0.5 400,276 + -1.0 400,276 + -0.3 400,276 + +1.0 400,307 + IAI47_20775 0.12 +0.7 400,307 + IAI47_20775 0.12 -0.2 400,359 + IAI47_20775 0.18 +1.9 400,360 - IAI47_20775 0.18 +0.2 400,360 - IAI47_20775 0.18 +2.4 400,434 + IAI47_20775 0.26 -1.7 400,435 - IAI47_20775 0.26 +0.1 400,435 - IAI47_20775 0.26 -0.3 400,435 - IAI47_20775 0.26 -0.7 400,435 - IAI47_20775 0.26 +0.7 400,465 - IAI47_20775 0.29 +0.3 400,515 + IAI47_20775 0.34 +2.4 400,516 - IAI47_20775 0.34 -0.2 400,553 + IAI47_20775 0.38 -0.4 400,554 - IAI47_20775 0.38 +1.6 400,554 - IAI47_20775 0.38 -0.3 400,569 + IAI47_20775 0.40 -0.6 400,569 + IAI47_20775 0.40 +0.1 400,569 + IAI47_20775 0.40 +0.6 400,582 + IAI47_20775 0.41 -1.2 400,590 + IAI47_20775 0.42 +2.1 400,590 + IAI47_20775 0.42 +0.4 400,590 + IAI47_20775 0.42 -2.7 400,591 - IAI47_20775 0.42 -1.1 400,602 + IAI47_20775 0.43 +1.1 400,602 + IAI47_20775 0.43 -1.5 400,603 - IAI47_20775 0.44 -0.5 400,603 - IAI47_20775 0.44 +0.7 400,603 - IAI47_20775 0.44 +0.9 400,708 + IAI47_20775 0.55 +0.2 400,708 + IAI47_20775 0.55 -1.9 400,709 - IAI47_20775 0.55 -1.4 400,855 - IAI47_20775 0.70 -0.1 400,992 + IAI47_20775 0.84 +0.5 400,992 + IAI47_20775 0.84 -0.3 400,992 + IAI47_20775 0.84 -0.5 400,993 - IAI47_20775 0.85 +2.0 400,993 - IAI47_20775 0.85 +1.7 400,993 - IAI47_20775 0.85 -0.4 400,993 - IAI47_20775 0.85 -1.9 400,993 - IAI47_20775 0.85 +1.3 400,993 - IAI47_20775 0.85 +0.7 401,015 + IAI47_20775 0.87 +0.4 401,015 + IAI47_20775 0.87 -1.1 401,015 + IAI47_20775 0.87 +0.4 401,015 + IAI47_20775 0.87 -0.1 401,015 + IAI47_20775 0.87 +1.2 401,016 - IAI47_20775 0.87 -1.4 401,016 - IAI47_20775 0.87 +0.2 401,049 + +1.0 401,050 - -0.2 401,050 - +2.3 401,050 - +0.7 401,056 + +0.6 401,056 + +0.8 401,057 - +1.1 401,057 - +0.3 401,057 - +0.1 401,057 - -0.3 401,085 + -1.6 401,085 + -0.5 401,085 + -1.3 401,085 + +0.4 401,086 - +1.6 401,086 - -0.6 401,115 + +0.1 401,116 - -0.2 401,137 + +0.5 401,138 - -0.3 401,157 + +1.1 401,158 - +0.3 401,180 + +1.0 401,180 + +1.4 401,180 + +0.5 401,180 + +2.3 401,180 + +0.4 401,181 - +0.3 401,181 - -0.4 401,203 + -0.0 401,204 - -0.5 401,204 - +1.8 401,204 - +0.1 401,204 - -2.7 401,204 - -0.3 401,204 - +0.8 401,213 + +0.7 401,214 - -0.6 401,256 + -0.8 401,256 + -2.1 401,256 + -0.5 401,256 + -0.0 401,256 + +0.8 401,256 + +1.9 401,256 + -0.2 401,256 + -0.7 401,256 + +0.6 401,256 + +0.3 401,256 + +1.2 401,256 + -0.9 401,256 + -0.0 401,256 + +0.1 401,256 + +0.2 401,256 + -0.2 401,256 + +0.6 401,257 - +0.6 401,257 - -0.1 401,257 - -1.2 401,257 - -0.3 401,257 - -0.0 401,257 - +1.3 401,257 - -0.7 401,257 - -0.3 401,257 - +1.9 401,260 + -0.6 401,260 + +1.1 401,260 + -0.6 401,260 + -0.2 401,260 + -0.9 401,260 + -0.2 401,260 + +0.1 401,260 + +0.1 401,260 + +0.3 401,260 + +0.9 401,260 + -0.9 401,260 + +1.1 401,260 + -0.8 401,261 - -0.0 401,261 - -0.7 401,261 - -1.6 401,261 - +2.0 401,261 - +0.2 401,261 - +0.4 401,261 - +0.2 401,286 + IAI47_20780 0.16 -0.2 401,389 + IAI47_20780 0.46 -0.9 401,389 + IAI47_20780 0.46 +0.3 401,389 + IAI47_20780 0.46 +0.3 401,389 + IAI47_20780 0.46 +1.8 401,390 - IAI47_20780 0.46 +0.3 401,390 - IAI47_20780 0.46 +0.3 401,390 - IAI47_20780 0.46 +0.4 401,405 + IAI47_20780 0.51 +1.7 401,405 + IAI47_20780 0.51 -0.5 401,405 + IAI47_20780 0.51 +0.2 401,405 + IAI47_20780 0.51 +0.4 401,406 - IAI47_20780 0.51 -0.2 401,406 - IAI47_20780 0.51 +0.9 401,406 - IAI47_20780 0.51 +1.2 401,406 - IAI47_20780 0.51 +0.4 401,602 + +0.0 401,602 + -0.9 401,602 + +0.2 401,603 - +1.5 401,648 + +1.3 401,683 + +2.7 401,683 + -0.9 401,683 + -1.6 401,684 - +1.0 401,684 - +1.1 401,684 - -0.3 401,789 + IAI47_20785 0.31 -1.4 401,790 - IAI47_20785 0.31 -0.2 401,790 - IAI47_20785 0.31 +0.2 401,807 + IAI47_20785 0.35 -0.0 401,808 - IAI47_20785 0.35 +0.3 401,808 - IAI47_20785 0.35 +0.6 401,825 + IAI47_20785 0.38 +2.3 401,826 - IAI47_20785 0.39 +0.6 401,826 - IAI47_20785 0.39 -0.4 401,826 - IAI47_20785 0.39 +0.3 401,888 + IAI47_20785 0.51 -0.4 401,888 + IAI47_20785 0.51 +1.4 401,889 - IAI47_20785 0.52 +0.7 401,890 + IAI47_20785 0.52 -0.1 401,906 - IAI47_20785 0.55 -0.2 401,913 - IAI47_20785 0.56 -0.2 401,913 - IAI47_20785 0.56 -0.0 401,997 - IAI47_20785 0.74 +0.1 401,997 - IAI47_20785 0.74 +0.7 402,004 + IAI47_20785 0.75 -1.1 402,005 - IAI47_20785 0.75 -0.0 402,029 + IAI47_20785 0.80 +0.7 402,030 - IAI47_20785 0.80 +0.8 402,039 - IAI47_20785 0.82 -0.6 402,043 + IAI47_20785 0.83 +0.1 402,046 + IAI47_20785 0.84 -1.3 402,046 + IAI47_20785 0.84 +0.1 402,046 + IAI47_20785 0.84 +2.3 402,046 + IAI47_20785 0.84 -2.2 402,046 + IAI47_20785 0.84 -1.3 402,047 - IAI47_20785 0.84 +0.2 402,047 - IAI47_20785 0.84 -1.4 402,049 + IAI47_20785 0.84 -1.3 402,049 + IAI47_20785 0.84 +0.5 402,050 - IAI47_20785 0.84 -0.0 402,050 - IAI47_20785 0.84 +0.9 402,050 - IAI47_20785 0.84 +0.4 402,050 - IAI47_20785 0.84 +0.6 402,050 - IAI47_20785 0.84 +0.5 402,050 - IAI47_20785 0.84 +1.1 402,117 + -1.8 402,117 + +1.2 402,118 - +0.2 402,129 + -0.0 402,129 + +0.4 402,145 - -2.6 402,147 + +0.2 402,148 - +0.3 402,148 - +0.3 402,148 - +1.3 402,176 + +0.6 402,177 - -0.8 402,179 + +2.3 402,179 + +1.2 402,180 - -0.7 402,180 - -0.0 402,180 - -0.3 402,186 + +0.1 402,186 + +0.9 402,209 + -1.8 402,210 - +0.2 402,218 + +1.2 402,228 + +0.0 402,229 - -1.9 402,229 - +1.5 402,264 + +3.3 402,277 - +1.0 402,282 + -1.2 402,301 + +1.2 402,301 + +1.0 402,302 - +0.7 402,302 - +0.7 402,312 + +0.2 402,313 - +1.4 402,313 - +1.5 402,361 + +0.4 402,361 + +0.1 402,362 - -2.6 402,362 - -0.2 402,403 - IAI47_20790 0.10 +0.5 402,403 - IAI47_20790 0.10 +0.6 402,451 - IAI47_20790 0.14 -0.2 402,468 + IAI47_20790 0.16 +2.0 402,468 + IAI47_20790 0.16 +0.3 402,482 + IAI47_20790 0.17 -0.6 402,482 + IAI47_20790 0.17 +0.6 402,483 - IAI47_20790 0.17 -0.8 402,555 + IAI47_20790 0.23 -0.4 402,555 + IAI47_20790 0.23 +1.2
Or see this region's nucleotide sequence