Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_05180 and IAI47_05185 are separated by 229 nucleotides IAI47_05185 and IAI47_05190 are separated by 137 nucleotides IAI47_05190 and IAI47_05195 are separated by 101 nucleotides IAI47_05195 and IAI47_05200 overlap by 4 nucleotides
IAI47_05180: IAI47_05180 - phosphoribosylformylglycinamidine cyclo-ligase, at 1,095,911 to 1,096,951
_05180
IAI47_05185: IAI47_05185 - uracil phosphoribosyltransferase, at 1,097,181 to 1,097,807
_05185
IAI47_05190: IAI47_05190 - uracil permease, at 1,097,945 to 1,099,228
_05190
IAI47_05195: IAI47_05195 - DnaA inactivator Hda, at 1,099,330 to 1,100,037
_05195
IAI47_05200: IAI47_05200 - arsenate reductase (glutaredoxin), at 1,100,034 to 1,100,375
_05200
Position (kb)
1097
1098
1099
1100 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1097.006 kb on + strand at 1097.007 kb on - strand at 1097.007 kb on - strand at 1097.062 kb on - strand at 1097.069 kb on + strand at 1097.069 kb on + strand at 1097.069 kb on + strand at 1097.069 kb on + strand at 1097.069 kb on + strand at 1097.070 kb on - strand at 1097.070 kb on - strand at 1097.070 kb on - strand at 1097.070 kb on - strand at 1097.070 kb on - strand at 1097.070 kb on - strand at 1097.081 kb on + strand at 1097.081 kb on + strand at 1097.132 kb on + strand at 1097.135 kb on - strand at 1097.135 kb on - strand at 1097.219 kb on + strand at 1097.219 kb on + strand at 1097.219 kb on + strand at 1097.219 kb on + strand at 1097.220 kb on - strand at 1097.220 kb on - strand at 1097.220 kb on - strand at 1097.220 kb on - strand at 1097.220 kb on - strand at 1097.246 kb on + strand, within IAI47_05185 at 1097.247 kb on - strand, within IAI47_05185 at 1097.301 kb on + strand, within IAI47_05185 at 1097.301 kb on + strand, within IAI47_05185 at 1097.301 kb on + strand, within IAI47_05185 at 1097.301 kb on + strand, within IAI47_05185 at 1097.302 kb on - strand, within IAI47_05185 at 1097.302 kb on - strand, within IAI47_05185 at 1097.302 kb on - strand, within IAI47_05185 at 1097.362 kb on + strand, within IAI47_05185 at 1097.484 kb on + strand, within IAI47_05185 at 1097.485 kb on - strand, within IAI47_05185 at 1097.485 kb on - strand, within IAI47_05185 at 1097.517 kb on + strand, within IAI47_05185 at 1097.517 kb on + strand, within IAI47_05185 at 1097.517 kb on + strand, within IAI47_05185 at 1097.517 kb on + strand, within IAI47_05185 at 1097.518 kb on - strand, within IAI47_05185 at 1097.537 kb on + strand, within IAI47_05185 at 1097.537 kb on + strand, within IAI47_05185 at 1097.537 kb on + strand, within IAI47_05185 at 1097.538 kb on - strand, within IAI47_05185 at 1097.538 kb on - strand, within IAI47_05185 at 1097.716 kb on - strand, within IAI47_05185 at 1097.716 kb on - strand, within IAI47_05185 at 1097.754 kb on + strand at 1097.754 kb on + strand at 1097.754 kb on + strand at 1097.755 kb on - strand at 1097.755 kb on - strand at 1097.755 kb on - strand at 1097.755 kb on - strand at 1097.755 kb on - strand at 1097.805 kb on + strand at 1097.806 kb on - strand at 1097.806 kb on - strand at 1097.939 kb on + strand at 1097.939 kb on + strand at 1097.940 kb on - strand at 1097.940 kb on - strand at 1097.940 kb on - strand at 1097.940 kb on - strand at 1097.940 kb on - strand at 1098.114 kb on - strand, within IAI47_05190 at 1098.114 kb on - strand, within IAI47_05190 at 1098.114 kb on - strand, within IAI47_05190 at 1098.121 kb on + strand, within IAI47_05190 at 1098.121 kb on + strand, within IAI47_05190 at 1098.121 kb on + strand, within IAI47_05190 at 1098.121 kb on + strand, within IAI47_05190 at 1098.122 kb on - strand, within IAI47_05190 at 1098.127 kb on + strand, within IAI47_05190 at 1098.128 kb on - strand, within IAI47_05190 at 1098.147 kb on - strand, within IAI47_05190 at 1098.147 kb on - strand, within IAI47_05190 at 1098.169 kb on + strand, within IAI47_05190 at 1098.186 kb on + strand, within IAI47_05190 at 1098.186 kb on + strand, within IAI47_05190 at 1098.187 kb on - strand, within IAI47_05190 at 1098.187 kb on - strand, within IAI47_05190 at 1098.187 kb on - strand, within IAI47_05190 at 1098.187 kb on - strand, within IAI47_05190 at 1098.204 kb on - strand, within IAI47_05190 at 1098.226 kb on + strand, within IAI47_05190 at 1098.226 kb on + strand, within IAI47_05190 at 1098.226 kb on + strand, within IAI47_05190 at 1098.227 kb on - strand, within IAI47_05190 at 1098.370 kb on + strand, within IAI47_05190 at 1098.413 kb on - strand, within IAI47_05190 at 1098.448 kb on - strand, within IAI47_05190 at 1098.538 kb on + strand, within IAI47_05190 at 1098.538 kb on + strand, within IAI47_05190 at 1098.599 kb on + strand, within IAI47_05190 at 1098.599 kb on + strand, within IAI47_05190 at 1098.600 kb on - strand, within IAI47_05190 at 1098.600 kb on - strand, within IAI47_05190 at 1098.684 kb on + strand, within IAI47_05190 at 1098.806 kb on + strand, within IAI47_05190 at 1098.806 kb on + strand, within IAI47_05190 at 1098.807 kb on - strand, within IAI47_05190 at 1098.807 kb on - strand, within IAI47_05190 at 1098.807 kb on - strand, within IAI47_05190 at 1098.807 kb on - strand, within IAI47_05190 at 1098.807 kb on - strand, within IAI47_05190 at 1098.845 kb on + strand, within IAI47_05190 at 1098.846 kb on - strand, within IAI47_05190 at 1098.968 kb on + strand, within IAI47_05190 at 1098.968 kb on + strand, within IAI47_05190 at 1098.968 kb on + strand, within IAI47_05190 at 1098.969 kb on - strand, within IAI47_05190 at 1098.969 kb on - strand, within IAI47_05190 at 1099.022 kb on + strand, within IAI47_05190 at 1099.022 kb on + strand, within IAI47_05190 at 1099.022 kb on + strand, within IAI47_05190 at 1099.023 kb on - strand, within IAI47_05190 at 1099.023 kb on - strand, within IAI47_05190 at 1099.094 kb on - strand, within IAI47_05190 at 1099.168 kb on + strand at 1099.293 kb on + strand at 1099.293 kb on + strand at 1099.293 kb on + strand at 1099.294 kb on - strand at 1099.370 kb on - strand at 1099.370 kb on - strand at 1099.468 kb on + strand, within IAI47_05195 at 1099.468 kb on + strand, within IAI47_05195 at 1099.469 kb on - strand, within IAI47_05195 at 1099.474 kb on + strand, within IAI47_05195 at 1099.698 kb on + strand, within IAI47_05195 at 1099.764 kb on + strand, within IAI47_05195 at 1099.847 kb on + strand, within IAI47_05195 at 1100.016 kb on + strand at 1100.036 kb on - strand at 1100.082 kb on - strand, within IAI47_05200 at 1100.097 kb on + strand, within IAI47_05200 at 1100.127 kb on + strand, within IAI47_05200 at 1100.128 kb on - strand, within IAI47_05200 at 1100.182 kb on + strand, within IAI47_05200 at 1100.183 kb on - strand, within IAI47_05200 at 1100.196 kb on - strand, within IAI47_05200 at 1100.196 kb on - strand, within IAI47_05200 at 1100.196 kb on - strand, within IAI47_05200 at 1100.205 kb on + strand, within IAI47_05200
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,097,006 + -2.0 1,097,007 - -1.4 1,097,007 - +0.0 1,097,062 - -0.7 1,097,069 + +1.0 1,097,069 + -1.1 1,097,069 + +1.4 1,097,069 + -0.5 1,097,069 + -0.9 1,097,070 - +1.4 1,097,070 - -0.6 1,097,070 - -1.1 1,097,070 - +0.8 1,097,070 - +0.3 1,097,070 - -1.4 1,097,081 + -0.2 1,097,081 + -1.4 1,097,132 + +0.1 1,097,135 - -0.4 1,097,135 - -2.1 1,097,219 + +1.6 1,097,219 + -1.3 1,097,219 + +0.2 1,097,219 + +0.6 1,097,220 - -0.4 1,097,220 - -1.0 1,097,220 - -0.1 1,097,220 - -2.4 1,097,220 - -1.8 1,097,246 + IAI47_05185 0.10 +0.4 1,097,247 - IAI47_05185 0.11 +1.6 1,097,301 + IAI47_05185 0.19 -0.9 1,097,301 + IAI47_05185 0.19 +0.1 1,097,301 + IAI47_05185 0.19 +0.1 1,097,301 + IAI47_05185 0.19 +0.1 1,097,302 - IAI47_05185 0.19 -0.1 1,097,302 - IAI47_05185 0.19 +1.7 1,097,302 - IAI47_05185 0.19 -1.0 1,097,362 + IAI47_05185 0.29 -2.4 1,097,484 + IAI47_05185 0.48 -0.0 1,097,485 - IAI47_05185 0.48 -0.0 1,097,485 - IAI47_05185 0.48 +0.9 1,097,517 + IAI47_05185 0.54 -1.9 1,097,517 + IAI47_05185 0.54 +0.1 1,097,517 + IAI47_05185 0.54 +0.9 1,097,517 + IAI47_05185 0.54 -0.2 1,097,518 - IAI47_05185 0.54 +0.8 1,097,537 + IAI47_05185 0.57 -1.9 1,097,537 + IAI47_05185 0.57 +1.2 1,097,537 + IAI47_05185 0.57 -1.9 1,097,538 - IAI47_05185 0.57 -0.5 1,097,538 - IAI47_05185 0.57 -2.1 1,097,716 - IAI47_05185 0.85 -1.2 1,097,716 - IAI47_05185 0.85 +4.1 1,097,754 + -1.9 1,097,754 + -0.3 1,097,754 + -1.8 1,097,755 - -1.3 1,097,755 - -0.0 1,097,755 - -0.1 1,097,755 - -0.7 1,097,755 - +1.0 1,097,805 + +1.8 1,097,806 - -1.9 1,097,806 - +0.1 1,097,939 + +0.7 1,097,939 + -0.1 1,097,940 - -1.4 1,097,940 - +1.0 1,097,940 - +0.1 1,097,940 - +0.6 1,097,940 - -0.6 1,098,114 - IAI47_05190 0.13 +1.9 1,098,114 - IAI47_05190 0.13 +1.0 1,098,114 - IAI47_05190 0.13 -0.7 1,098,121 + IAI47_05190 0.14 -0.3 1,098,121 + IAI47_05190 0.14 -0.1 1,098,121 + IAI47_05190 0.14 -0.6 1,098,121 + IAI47_05190 0.14 +0.4 1,098,122 - IAI47_05190 0.14 -0.4 1,098,127 + IAI47_05190 0.14 -0.6 1,098,128 - IAI47_05190 0.14 -0.6 1,098,147 - IAI47_05190 0.16 +2.1 1,098,147 - IAI47_05190 0.16 +2.1 1,098,169 + IAI47_05190 0.17 -0.8 1,098,186 + IAI47_05190 0.19 +0.4 1,098,186 + IAI47_05190 0.19 -0.1 1,098,187 - IAI47_05190 0.19 -2.2 1,098,187 - IAI47_05190 0.19 -1.5 1,098,187 - IAI47_05190 0.19 +0.0 1,098,187 - IAI47_05190 0.19 -0.3 1,098,204 - IAI47_05190 0.20 +0.1 1,098,226 + IAI47_05190 0.22 +0.5 1,098,226 + IAI47_05190 0.22 -1.5 1,098,226 + IAI47_05190 0.22 -1.5 1,098,227 - IAI47_05190 0.22 +0.8 1,098,370 + IAI47_05190 0.33 +0.0 1,098,413 - IAI47_05190 0.36 -2.2 1,098,448 - IAI47_05190 0.39 -1.8 1,098,538 + IAI47_05190 0.46 +0.7 1,098,538 + IAI47_05190 0.46 -1.8 1,098,599 + IAI47_05190 0.51 -0.2 1,098,599 + IAI47_05190 0.51 -1.1 1,098,600 - IAI47_05190 0.51 +1.1 1,098,600 - IAI47_05190 0.51 -1.0 1,098,684 + IAI47_05190 0.58 +0.1 1,098,806 + IAI47_05190 0.67 -0.1 1,098,806 + IAI47_05190 0.67 -0.6 1,098,807 - IAI47_05190 0.67 +0.9 1,098,807 - IAI47_05190 0.67 -0.2 1,098,807 - IAI47_05190 0.67 -2.0 1,098,807 - IAI47_05190 0.67 -0.6 1,098,807 - IAI47_05190 0.67 +0.6 1,098,845 + IAI47_05190 0.70 -0.6 1,098,846 - IAI47_05190 0.70 +0.7 1,098,968 + IAI47_05190 0.80 -0.3 1,098,968 + IAI47_05190 0.80 +0.5 1,098,968 + IAI47_05190 0.80 -0.7 1,098,969 - IAI47_05190 0.80 -0.4 1,098,969 - IAI47_05190 0.80 +0.4 1,099,022 + IAI47_05190 0.84 -0.4 1,099,022 + IAI47_05190 0.84 -1.3 1,099,022 + IAI47_05190 0.84 -0.3 1,099,023 - IAI47_05190 0.84 +2.6 1,099,023 - IAI47_05190 0.84 +0.6 1,099,094 - IAI47_05190 0.89 -0.3 1,099,168 + +0.7 1,099,293 + -0.5 1,099,293 + +0.1 1,099,293 + -0.2 1,099,294 - -0.0 1,099,370 - -1.0 1,099,370 - -0.9 1,099,468 + IAI47_05195 0.19 -1.8 1,099,468 + IAI47_05195 0.19 -1.4 1,099,469 - IAI47_05195 0.20 -1.0 1,099,474 + IAI47_05195 0.20 +0.4 1,099,698 + IAI47_05195 0.52 -0.4 1,099,764 + IAI47_05195 0.61 +0.2 1,099,847 + IAI47_05195 0.73 -1.7 1,100,016 + -0.6 1,100,036 - -0.0 1,100,082 - IAI47_05200 0.14 +2.3 1,100,097 + IAI47_05200 0.18 -0.4 1,100,127 + IAI47_05200 0.27 -0.4 1,100,128 - IAI47_05200 0.27 +0.3 1,100,182 + IAI47_05200 0.43 +1.7 1,100,183 - IAI47_05200 0.44 +0.9 1,100,196 - IAI47_05200 0.47 -1.5 1,100,196 - IAI47_05200 0.47 -0.1 1,100,196 - IAI47_05200 0.47 -0.9 1,100,205 + IAI47_05200 0.50 +1.8
Or see this region's nucleotide sequence