Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_02295 and IAI47_02300 overlap by 29 nucleotides IAI47_02300 and IAI47_02305 are separated by 24 nucleotides IAI47_02305 and IAI47_02310 are separated by 3 nucleotides IAI47_02310 and IAI47_02315 are separated by 3 nucleotides
IAI47_02295: IAI47_02295 - DNA-protecting protein DprA, at 475,703 to 476,827
_02295
IAI47_02300: IAI47_02300 - DUF494 domain-containing protein, at 476,799 to 477,272
_02300
IAI47_02305: IAI47_02305 - topoisomerase DNA-binding C4 zinc finger domain-containing protein, at 477,297 to 477,845
_02305
IAI47_02310: IAI47_02310 - L-threonylcarbamoyladenylate synthase type 1 TsaC, at 477,849 to 478,421
_02310
IAI47_02315: IAI47_02315 - shikimate dehydrogenase, at 478,425 to 479,243
_02315
Position (kb) 477
478 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 476.341 kb on + strand, within IAI47_02295 at 476.341 kb on + strand, within IAI47_02295 at 476.341 kb on + strand, within IAI47_02295 at 476.342 kb on - strand, within IAI47_02295 at 476.376 kb on + strand, within IAI47_02295 at 476.376 kb on + strand, within IAI47_02295 at 476.376 kb on + strand, within IAI47_02295 at 476.376 kb on + strand, within IAI47_02295 at 476.376 kb on + strand, within IAI47_02295 at 476.376 kb on + strand, within IAI47_02295 at 476.376 kb on + strand, within IAI47_02295 at 476.377 kb on - strand, within IAI47_02295 at 476.377 kb on - strand, within IAI47_02295 at 476.418 kb on - strand, within IAI47_02295 at 476.418 kb on - strand, within IAI47_02295 at 476.444 kb on + strand, within IAI47_02295 at 476.444 kb on + strand, within IAI47_02295 at 476.444 kb on + strand, within IAI47_02295 at 476.444 kb on + strand, within IAI47_02295 at 476.444 kb on + strand, within IAI47_02295 at 476.444 kb on + strand, within IAI47_02295 at 476.445 kb on - strand, within IAI47_02295 at 476.445 kb on - strand, within IAI47_02295 at 476.445 kb on - strand, within IAI47_02295 at 476.445 kb on - strand, within IAI47_02295 at 476.445 kb on - strand, within IAI47_02295 at 476.445 kb on - strand, within IAI47_02295 at 476.445 kb on - strand, within IAI47_02295 at 476.451 kb on + strand, within IAI47_02295 at 476.452 kb on - strand, within IAI47_02295 at 476.452 kb on - strand, within IAI47_02295 at 476.452 kb on - strand, within IAI47_02295 at 476.452 kb on - strand, within IAI47_02295 at 476.452 kb on - strand, within IAI47_02295 at 476.489 kb on - strand, within IAI47_02295 at 476.534 kb on - strand, within IAI47_02295 at 476.534 kb on - strand, within IAI47_02295 at 476.648 kb on - strand, within IAI47_02295 at 476.652 kb on + strand, within IAI47_02295 at 476.652 kb on + strand, within IAI47_02295 at 476.652 kb on + strand, within IAI47_02295 at 476.652 kb on + strand, within IAI47_02295 at 476.652 kb on + strand, within IAI47_02295 at 476.652 kb on + strand, within IAI47_02295 at 476.652 kb on + strand, within IAI47_02295 at 476.652 kb on + strand, within IAI47_02295 at 476.652 kb on + strand, within IAI47_02295 at 476.652 kb on + strand, within IAI47_02295 at 476.653 kb on - strand, within IAI47_02295 at 476.653 kb on - strand, within IAI47_02295 at 476.653 kb on - strand, within IAI47_02295 at 476.653 kb on - strand, within IAI47_02295 at 476.653 kb on - strand, within IAI47_02295 at 476.653 kb on - strand, within IAI47_02295 at 476.653 kb on - strand, within IAI47_02295 at 476.653 kb on - strand, within IAI47_02295 at 476.665 kb on + strand, within IAI47_02295 at 476.665 kb on + strand, within IAI47_02295 at 476.665 kb on + strand, within IAI47_02295 at 476.665 kb on + strand, within IAI47_02295 at 476.665 kb on + strand, within IAI47_02295 at 476.665 kb on + strand, within IAI47_02295 at 476.666 kb on - strand, within IAI47_02295 at 476.666 kb on - strand, within IAI47_02295 at 476.666 kb on - strand, within IAI47_02295 at 476.666 kb on - strand, within IAI47_02295 at 476.713 kb on + strand, within IAI47_02295 at 476.713 kb on + strand, within IAI47_02295 at 476.713 kb on + strand, within IAI47_02295 at 476.714 kb on - strand, within IAI47_02295 at 476.714 kb on - strand, within IAI47_02295 at 476.743 kb on - strand at 476.743 kb on - strand at 476.774 kb on + strand at 476.775 kb on - strand at 476.775 kb on - strand at 476.809 kb on + strand at 476.809 kb on + strand at 476.809 kb on + strand at 476.809 kb on + strand at 476.809 kb on + strand at 476.810 kb on - strand at 476.810 kb on - strand at 476.810 kb on - strand at 476.810 kb on - strand at 476.817 kb on + strand at 476.817 kb on + strand at 476.818 kb on - strand at 476.818 kb on - strand at 476.818 kb on - strand at 476.818 kb on - strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.832 kb on + strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.833 kb on - strand at 476.834 kb on + strand at 476.834 kb on + strand at 476.834 kb on + strand at 476.834 kb on + strand at 476.834 kb on + strand at 476.834 kb on + strand at 476.835 kb on - strand at 476.835 kb on - strand at 476.835 kb on - strand at 476.835 kb on - strand at 476.835 kb on - strand at 476.835 kb on - strand at 476.835 kb on - strand at 476.835 kb on - strand at 476.835 kb on - strand at 476.835 kb on - strand at 476.835 kb on - strand at 476.835 kb on - strand at 476.835 kb on - strand at 476.835 kb on - strand at 476.919 kb on + strand, within IAI47_02300 at 476.919 kb on + strand, within IAI47_02300 at 476.919 kb on + strand, within IAI47_02300 at 476.919 kb on + strand, within IAI47_02300 at 476.919 kb on + strand, within IAI47_02300 at 476.920 kb on - strand, within IAI47_02300 at 476.920 kb on - strand, within IAI47_02300 at 477.011 kb on + strand, within IAI47_02300 at 477.011 kb on + strand, within IAI47_02300 at 477.011 kb on + strand, within IAI47_02300 at 477.011 kb on + strand, within IAI47_02300 at 477.011 kb on + strand, within IAI47_02300 at 477.012 kb on - strand, within IAI47_02300 at 477.012 kb on - strand, within IAI47_02300 at 477.012 kb on - strand, within IAI47_02300 at 477.012 kb on - strand, within IAI47_02300 at 477.012 kb on - strand, within IAI47_02300 at 477.012 kb on - strand, within IAI47_02300 at 477.012 kb on - strand, within IAI47_02300 at 477.015 kb on + strand, within IAI47_02300 at 477.015 kb on + strand, within IAI47_02300 at 477.015 kb on + strand, within IAI47_02300 at 477.015 kb on + strand, within IAI47_02300 at 477.015 kb on + strand, within IAI47_02300 at 477.015 kb on + strand, within IAI47_02300 at 477.015 kb on + strand, within IAI47_02300 at 477.016 kb on - strand, within IAI47_02300 at 477.016 kb on - strand, within IAI47_02300 at 477.016 kb on - strand, within IAI47_02300 at 477.016 kb on - strand, within IAI47_02300 at 477.016 kb on - strand, within IAI47_02300 at 477.040 kb on + strand, within IAI47_02300 at 477.040 kb on + strand, within IAI47_02300 at 477.040 kb on + strand, within IAI47_02300 at 477.041 kb on - strand, within IAI47_02300 at 477.050 kb on + strand, within IAI47_02300 at 477.050 kb on + strand, within IAI47_02300 at 477.050 kb on + strand, within IAI47_02300 at 477.125 kb on + strand, within IAI47_02300 at 477.125 kb on + strand, within IAI47_02300 at 477.125 kb on + strand, within IAI47_02300 at 477.125 kb on + strand, within IAI47_02300 at 477.126 kb on - strand, within IAI47_02300 at 477.126 kb on - strand, within IAI47_02300 at 477.126 kb on - strand, within IAI47_02300 at 477.140 kb on + strand, within IAI47_02300 at 477.140 kb on + strand, within IAI47_02300 at 477.141 kb on - strand, within IAI47_02300 at 477.220 kb on - strand, within IAI47_02300 at 477.241 kb on + strand at 477.241 kb on + strand at 477.242 kb on - strand at 477.242 kb on - strand at 477.242 kb on - strand at 477.260 kb on + strand at 477.260 kb on + strand at 477.260 kb on + strand at 477.260 kb on + strand at 477.260 kb on + strand at 477.260 kb on + strand at 477.260 kb on + strand at 477.260 kb on + strand at 477.260 kb on + strand at 477.260 kb on + strand at 477.260 kb on + strand at 477.261 kb on - strand at 477.261 kb on - strand at 477.261 kb on - strand at 477.261 kb on - strand at 477.270 kb on + strand at 477.297 kb on - strand at 477.302 kb on + strand at 477.302 kb on + strand at 477.302 kb on + strand at 477.302 kb on + strand at 477.326 kb on + strand at 477.326 kb on + strand at 477.326 kb on + strand at 477.327 kb on - strand at 477.409 kb on - strand, within IAI47_02305 at 477.424 kb on - strand, within IAI47_02305 at 477.424 kb on - strand, within IAI47_02305 at 477.436 kb on + strand, within IAI47_02305 at 477.437 kb on - strand, within IAI47_02305 at 477.437 kb on - strand, within IAI47_02305 at 477.437 kb on - strand, within IAI47_02305 at 477.539 kb on + strand, within IAI47_02305 at 477.570 kb on + strand, within IAI47_02305 at 477.637 kb on + strand, within IAI47_02305 at 477.637 kb on + strand, within IAI47_02305 at 477.637 kb on + strand, within IAI47_02305 at 477.741 kb on + strand, within IAI47_02305 at 477.741 kb on + strand, within IAI47_02305 at 477.742 kb on - strand, within IAI47_02305 at 477.742 kb on - strand, within IAI47_02305 at 477.742 kb on - strand, within IAI47_02305 at 477.760 kb on + strand, within IAI47_02305 at 477.761 kb on - strand, within IAI47_02305 at 477.788 kb on + strand, within IAI47_02305 at 477.788 kb on + strand, within IAI47_02305 at 477.806 kb on + strand at 477.806 kb on + strand at 477.806 kb on + strand at 477.843 kb on + strand at 477.843 kb on + strand at 477.843 kb on + strand at 477.843 kb on + strand at 478.419 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 476,341 + IAI47_02295 0.57 +0.3 476,341 + IAI47_02295 0.57 -0.7 476,341 + IAI47_02295 0.57 -0.9 476,342 - IAI47_02295 0.57 -2.7 476,376 + IAI47_02295 0.60 +0.7 476,376 + IAI47_02295 0.60 -0.0 476,376 + IAI47_02295 0.60 -0.5 476,376 + IAI47_02295 0.60 -2.2 476,376 + IAI47_02295 0.60 -0.0 476,376 + IAI47_02295 0.60 -1.3 476,376 + IAI47_02295 0.60 +0.5 476,377 - IAI47_02295 0.60 +0.1 476,377 - IAI47_02295 0.60 +0.8 476,418 - IAI47_02295 0.64 -0.2 476,418 - IAI47_02295 0.64 -0.3 476,444 + IAI47_02295 0.66 -0.3 476,444 + IAI47_02295 0.66 +1.9 476,444 + IAI47_02295 0.66 +0.9 476,444 + IAI47_02295 0.66 +3.9 476,444 + IAI47_02295 0.66 -0.3 476,444 + IAI47_02295 0.66 +0.1 476,445 - IAI47_02295 0.66 +1.3 476,445 - IAI47_02295 0.66 -0.7 476,445 - IAI47_02295 0.66 +1.0 476,445 - IAI47_02295 0.66 +0.3 476,445 - IAI47_02295 0.66 -0.9 476,445 - IAI47_02295 0.66 +2.4 476,445 - IAI47_02295 0.66 -0.0 476,451 + IAI47_02295 0.66 -0.4 476,452 - IAI47_02295 0.67 -0.9 476,452 - IAI47_02295 0.67 -1.1 476,452 - IAI47_02295 0.67 +3.3 476,452 - IAI47_02295 0.67 -2.0 476,452 - IAI47_02295 0.67 -0.4 476,489 - IAI47_02295 0.70 -0.6 476,534 - IAI47_02295 0.74 -0.5 476,534 - IAI47_02295 0.74 +0.8 476,648 - IAI47_02295 0.84 -0.2 476,652 + IAI47_02295 0.84 -1.0 476,652 + IAI47_02295 0.84 -0.9 476,652 + IAI47_02295 0.84 -0.2 476,652 + IAI47_02295 0.84 -1.0 476,652 + IAI47_02295 0.84 +0.3 476,652 + IAI47_02295 0.84 +0.7 476,652 + IAI47_02295 0.84 +1.5 476,652 + IAI47_02295 0.84 -1.2 476,652 + IAI47_02295 0.84 -0.4 476,652 + IAI47_02295 0.84 -1.1 476,653 - IAI47_02295 0.84 -0.8 476,653 - IAI47_02295 0.84 +2.0 476,653 - IAI47_02295 0.84 -1.3 476,653 - IAI47_02295 0.84 +0.1 476,653 - IAI47_02295 0.84 +2.5 476,653 - IAI47_02295 0.84 -1.9 476,653 - IAI47_02295 0.84 -1.5 476,653 - IAI47_02295 0.84 -0.8 476,665 + IAI47_02295 0.86 +0.5 476,665 + IAI47_02295 0.86 -2.5 476,665 + IAI47_02295 0.86 +1.4 476,665 + IAI47_02295 0.86 +1.8 476,665 + IAI47_02295 0.86 +1.4 476,665 + IAI47_02295 0.86 +0.0 476,666 - IAI47_02295 0.86 +2.1 476,666 - IAI47_02295 0.86 -0.7 476,666 - IAI47_02295 0.86 -0.7 476,666 - IAI47_02295 0.86 -0.2 476,713 + IAI47_02295 0.90 -0.4 476,713 + IAI47_02295 0.90 +1.5 476,713 + IAI47_02295 0.90 +0.0 476,714 - IAI47_02295 0.90 -0.2 476,714 - IAI47_02295 0.90 +0.5 476,743 - +1.4 476,743 - -2.6 476,774 + -0.1 476,775 - +1.5 476,775 - -0.9 476,809 + +1.3 476,809 + -0.1 476,809 + -0.6 476,809 + +0.1 476,809 + +0.1 476,810 - -0.1 476,810 - -0.2 476,810 - -0.4 476,810 - +0.3 476,817 + +0.6 476,817 + +0.3 476,818 - +0.9 476,818 - -1.1 476,818 - -0.1 476,818 - -1.3 476,832 + -0.5 476,832 + -0.9 476,832 + -0.9 476,832 + -0.1 476,832 + +1.0 476,832 + +1.2 476,832 + -1.7 476,832 + +0.6 476,832 + -0.5 476,832 + -0.6 476,832 + -0.6 476,832 + -1.2 476,832 + +0.5 476,832 + -1.3 476,832 + +0.1 476,832 + +1.0 476,832 + +0.1 476,832 + -0.8 476,832 + -2.3 476,832 + +0.5 476,832 + -0.5 476,832 + +1.1 476,832 + +2.8 476,832 + -1.9 476,832 + -0.4 476,832 + +1.2 476,832 + +1.1 476,832 + +0.1 476,832 + -0.8 476,832 + -0.4 476,832 + +0.5 476,833 - -0.0 476,833 - +1.3 476,833 - -0.0 476,833 - +0.4 476,833 - +0.5 476,833 - +0.5 476,833 - -0.6 476,833 - +0.4 476,833 - -2.5 476,833 - +2.9 476,833 - +1.3 476,833 - +0.9 476,833 - -0.7 476,833 - +0.1 476,833 - +0.6 476,833 - +0.7 476,833 - -0.3 476,833 - +0.4 476,833 - -2.0 476,833 - -0.3 476,833 - +1.6 476,833 - +0.3 476,833 - -0.0 476,833 - -0.9 476,833 - -1.7 476,833 - -0.7 476,833 - +1.2 476,833 - -2.0 476,834 + +0.4 476,834 + -0.7 476,834 + -0.4 476,834 + +1.8 476,834 + -0.6 476,834 + -0.4 476,835 - -0.9 476,835 - -0.0 476,835 - -0.1 476,835 - -0.3 476,835 - -0.3 476,835 - -1.1 476,835 - -2.5 476,835 - +0.6 476,835 - -0.7 476,835 - -0.9 476,835 - +1.1 476,835 - +0.6 476,835 - -0.0 476,835 - -0.4 476,919 + IAI47_02300 0.25 -1.3 476,919 + IAI47_02300 0.25 +1.2 476,919 + IAI47_02300 0.25 -0.1 476,919 + IAI47_02300 0.25 +1.2 476,919 + IAI47_02300 0.25 +1.8 476,920 - IAI47_02300 0.26 -0.3 476,920 - IAI47_02300 0.26 +1.3 477,011 + IAI47_02300 0.45 +0.5 477,011 + IAI47_02300 0.45 +2.0 477,011 + IAI47_02300 0.45 -0.0 477,011 + IAI47_02300 0.45 +1.0 477,011 + IAI47_02300 0.45 -0.2 477,012 - IAI47_02300 0.45 +2.1 477,012 - IAI47_02300 0.45 -0.1 477,012 - IAI47_02300 0.45 -1.3 477,012 - IAI47_02300 0.45 -0.3 477,012 - IAI47_02300 0.45 +0.6 477,012 - IAI47_02300 0.45 -0.4 477,012 - IAI47_02300 0.45 -1.6 477,015 + IAI47_02300 0.46 +0.5 477,015 + IAI47_02300 0.46 -1.1 477,015 + IAI47_02300 0.46 -0.6 477,015 + IAI47_02300 0.46 -0.5 477,015 + IAI47_02300 0.46 +0.1 477,015 + IAI47_02300 0.46 -0.2 477,015 + IAI47_02300 0.46 +1.9 477,016 - IAI47_02300 0.46 +0.0 477,016 - IAI47_02300 0.46 +1.7 477,016 - IAI47_02300 0.46 +0.2 477,016 - IAI47_02300 0.46 -0.2 477,016 - IAI47_02300 0.46 -1.1 477,040 + IAI47_02300 0.51 +0.6 477,040 + IAI47_02300 0.51 +0.2 477,040 + IAI47_02300 0.51 -1.3 477,041 - IAI47_02300 0.51 +2.2 477,050 + IAI47_02300 0.53 +0.5 477,050 + IAI47_02300 0.53 -0.5 477,050 + IAI47_02300 0.53 -0.1 477,125 + IAI47_02300 0.69 +0.3 477,125 + IAI47_02300 0.69 -0.3 477,125 + IAI47_02300 0.69 +1.6 477,125 + IAI47_02300 0.69 +1.5 477,126 - IAI47_02300 0.69 +0.3 477,126 - IAI47_02300 0.69 -2.0 477,126 - IAI47_02300 0.69 -0.6 477,140 + IAI47_02300 0.72 +0.8 477,140 + IAI47_02300 0.72 +0.2 477,141 - IAI47_02300 0.72 -0.6 477,220 - IAI47_02300 0.89 +0.6 477,241 + +0.5 477,241 + +1.5 477,242 - +0.8 477,242 - -0.9 477,242 - -0.0 477,260 + -0.5 477,260 + -2.2 477,260 + +1.5 477,260 + -0.2 477,260 + +0.8 477,260 + -0.0 477,260 + +0.4 477,260 + +0.0 477,260 + +0.0 477,260 + +0.6 477,260 + +0.4 477,261 - +2.2 477,261 - -1.1 477,261 - +1.8 477,261 - +0.2 477,270 + +0.2 477,297 - -1.3 477,302 + -2.1 477,302 + -2.4 477,302 + -1.0 477,302 + +0.8 477,326 + +1.2 477,326 + +0.0 477,326 + -0.5 477,327 - +1.2 477,409 - IAI47_02305 0.20 +0.8 477,424 - IAI47_02305 0.23 +0.8 477,424 - IAI47_02305 0.23 -0.8 477,436 + IAI47_02305 0.25 +1.1 477,437 - IAI47_02305 0.26 +1.4 477,437 - IAI47_02305 0.26 -2.0 477,437 - IAI47_02305 0.26 +0.8 477,539 + IAI47_02305 0.44 -0.2 477,570 + IAI47_02305 0.50 +0.5 477,637 + IAI47_02305 0.62 +0.8 477,637 + IAI47_02305 0.62 -1.5 477,637 + IAI47_02305 0.62 +0.6 477,741 + IAI47_02305 0.81 +1.2 477,741 + IAI47_02305 0.81 -0.4 477,742 - IAI47_02305 0.81 +0.5 477,742 - IAI47_02305 0.81 +1.2 477,742 - IAI47_02305 0.81 +0.5 477,760 + IAI47_02305 0.84 +0.2 477,761 - IAI47_02305 0.85 -2.1 477,788 + IAI47_02305 0.89 -0.2 477,788 + IAI47_02305 0.89 +1.2 477,806 + +1.2 477,806 + -0.7 477,806 + -1.1 477,843 + -0.1 477,843 + +0.4 477,843 + -0.1 477,843 + +0.1 478,419 + -0.7
Or see this region's nucleotide sequence