Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_12510 and IAI47_12515 overlap by 1 nucleotides IAI47_12515 and IAI47_12520 are separated by 20 nucleotides IAI47_12520 and IAI47_12525 overlap by 1 nucleotides IAI47_12525 and IAI47_12530 are separated by 19 nucleotides
IAI47_12510: IAI47_12510 - pyrimidine utilization protein A, at 2,636,790 to 2,637,881
_12510
IAI47_12515: IAI47_12515 - pyrimidine utilization protein B, at 2,637,881 to 2,638,594
_12515
IAI47_12520: IAI47_12520 - pyrimidine utilization protein C, at 2,638,615 to 2,639,010
_12520
IAI47_12525: IAI47_12525 - pyrimidine utilization protein D, at 2,639,010 to 2,639,819
_12525
IAI47_12530: IAI47_12530 - pyrimidine utilization flavin reductase protein F, at 2,639,839 to 2,640,354
_12530
Position (kb)
2638
2639
2640 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2637.649 kb on + strand, within IAI47_12510 at 2637.649 kb on + strand, within IAI47_12510 at 2637.649 kb on + strand, within IAI47_12510 at 2637.649 kb on + strand, within IAI47_12510 at 2637.649 kb on + strand, within IAI47_12510 at 2637.649 kb on + strand, within IAI47_12510 at 2637.650 kb on - strand, within IAI47_12510 at 2637.683 kb on + strand, within IAI47_12510 at 2637.683 kb on + strand, within IAI47_12510 at 2637.683 kb on + strand, within IAI47_12510 at 2637.684 kb on - strand, within IAI47_12510 at 2637.721 kb on + strand, within IAI47_12510 at 2637.751 kb on + strand, within IAI47_12510 at 2637.751 kb on + strand, within IAI47_12510 at 2637.752 kb on - strand, within IAI47_12510 at 2637.752 kb on - strand, within IAI47_12510 at 2637.752 kb on - strand, within IAI47_12510 at 2637.752 kb on - strand, within IAI47_12510 at 2637.800 kb on + strand at 2637.800 kb on + strand at 2637.845 kb on + strand at 2637.846 kb on - strand at 2637.846 kb on - strand at 2637.893 kb on + strand at 2637.904 kb on + strand at 2637.991 kb on + strand, within IAI47_12515 at 2637.992 kb on - strand, within IAI47_12515 at 2638.004 kb on + strand, within IAI47_12515 at 2638.005 kb on - strand, within IAI47_12515 at 2638.022 kb on + strand, within IAI47_12515 at 2638.023 kb on - strand, within IAI47_12515 at 2638.151 kb on + strand, within IAI47_12515 at 2638.151 kb on + strand, within IAI47_12515 at 2638.151 kb on + strand, within IAI47_12515 at 2638.151 kb on + strand, within IAI47_12515 at 2638.151 kb on + strand, within IAI47_12515 at 2638.152 kb on - strand, within IAI47_12515 at 2638.152 kb on - strand, within IAI47_12515 at 2638.152 kb on - strand, within IAI47_12515 at 2638.152 kb on - strand, within IAI47_12515 at 2638.180 kb on + strand, within IAI47_12515 at 2638.204 kb on - strand, within IAI47_12515 at 2638.337 kb on + strand, within IAI47_12515 at 2638.337 kb on + strand, within IAI47_12515 at 2638.337 kb on + strand, within IAI47_12515 at 2638.338 kb on - strand, within IAI47_12515 at 2638.338 kb on - strand, within IAI47_12515 at 2638.338 kb on - strand, within IAI47_12515 at 2638.338 kb on - strand, within IAI47_12515 at 2638.338 kb on - strand, within IAI47_12515 at 2638.340 kb on - strand, within IAI47_12515 at 2638.431 kb on + strand, within IAI47_12515 at 2638.454 kb on + strand, within IAI47_12515 at 2638.454 kb on + strand, within IAI47_12515 at 2638.454 kb on + strand, within IAI47_12515 at 2638.455 kb on - strand, within IAI47_12515 at 2638.484 kb on + strand, within IAI47_12515 at 2638.484 kb on + strand, within IAI47_12515 at 2638.484 kb on + strand, within IAI47_12515 at 2638.484 kb on + strand, within IAI47_12515 at 2638.484 kb on + strand, within IAI47_12515 at 2638.484 kb on + strand, within IAI47_12515 at 2638.485 kb on - strand, within IAI47_12515 at 2638.564 kb on + strand at 2638.565 kb on - strand at 2638.601 kb on - strand at 2638.601 kb on - strand at 2638.614 kb on + strand at 2638.614 kb on + strand at 2638.614 kb on + strand at 2638.615 kb on - strand at 2638.615 kb on - strand at 2638.615 kb on - strand at 2638.626 kb on + strand at 2638.626 kb on + strand at 2638.626 kb on + strand at 2638.627 kb on - strand at 2638.627 kb on - strand at 2638.627 kb on - strand at 2638.627 kb on - strand at 2638.657 kb on - strand, within IAI47_12520 at 2638.657 kb on - strand, within IAI47_12520 at 2638.657 kb on - strand, within IAI47_12520 at 2638.657 kb on - strand, within IAI47_12520 at 2638.699 kb on + strand, within IAI47_12520 at 2638.699 kb on + strand, within IAI47_12520 at 2638.699 kb on + strand at 2638.699 kb on + strand, within IAI47_12520 at 2638.700 kb on - strand, within IAI47_12520 at 2638.700 kb on - strand, within IAI47_12520 at 2638.700 kb on - strand, within IAI47_12520 at 2638.700 kb on - strand, within IAI47_12520 at 2638.700 kb on - strand, within IAI47_12520 at 2638.710 kb on + strand, within IAI47_12520 at 2638.711 kb on - strand, within IAI47_12520 at 2638.711 kb on - strand, within IAI47_12520 at 2638.711 kb on - strand, within IAI47_12520 at 2638.711 kb on - strand, within IAI47_12520 at 2638.711 kb on - strand, within IAI47_12520 at 2638.791 kb on + strand, within IAI47_12520 at 2638.791 kb on + strand, within IAI47_12520 at 2638.839 kb on + strand, within IAI47_12520 at 2638.870 kb on + strand, within IAI47_12520 at 2638.871 kb on - strand, within IAI47_12520 at 2638.871 kb on - strand, within IAI47_12520 at 2638.891 kb on + strand, within IAI47_12520 at 2638.900 kb on + strand, within IAI47_12520 at 2638.900 kb on + strand, within IAI47_12520 at 2638.901 kb on - strand, within IAI47_12520 at 2639.142 kb on + strand, within IAI47_12525 at 2639.142 kb on + strand, within IAI47_12525 at 2639.143 kb on - strand, within IAI47_12525 at 2639.143 kb on - strand, within IAI47_12525 at 2639.143 kb on - strand, within IAI47_12525 at 2639.190 kb on + strand, within IAI47_12525 at 2639.190 kb on + strand, within IAI47_12525 at 2639.190 kb on + strand, within IAI47_12525 at 2639.338 kb on + strand, within IAI47_12525 at 2639.338 kb on + strand, within IAI47_12525 at 2639.339 kb on - strand, within IAI47_12525 at 2639.339 kb on - strand, within IAI47_12525 at 2639.339 kb on - strand, within IAI47_12525 at 2639.343 kb on - strand, within IAI47_12525 at 2639.343 kb on - strand, within IAI47_12525 at 2639.343 kb on - strand, within IAI47_12525 at 2639.389 kb on + strand, within IAI47_12525 at 2639.390 kb on - strand, within IAI47_12525 at 2639.505 kb on - strand, within IAI47_12525 at 2639.817 kb on + strand at 2639.817 kb on + strand at 2639.838 kb on + strand at 2639.838 kb on + strand at 2639.838 kb on + strand at 2639.838 kb on + strand at 2639.838 kb on + strand at 2639.839 kb on - strand at 2639.839 kb on - strand at 2639.839 kb on - strand at 2639.839 kb on - strand at 2639.856 kb on + strand at 2639.922 kb on + strand, within IAI47_12530 at 2639.922 kb on + strand, within IAI47_12530 at 2639.922 kb on + strand, within IAI47_12530 at 2639.922 kb on + strand, within IAI47_12530 at 2639.922 kb on + strand, within IAI47_12530 at 2639.922 kb on + strand, within IAI47_12530 at 2639.922 kb on + strand, within IAI47_12530 at 2639.923 kb on - strand, within IAI47_12530 at 2639.923 kb on - strand, within IAI47_12530 at 2639.923 kb on - strand, within IAI47_12530 at 2639.923 kb on - strand, within IAI47_12530 at 2639.925 kb on + strand, within IAI47_12530 at 2639.925 kb on + strand, within IAI47_12530 at 2639.925 kb on + strand, within IAI47_12530 at 2639.925 kb on + strand, within IAI47_12530 at 2639.925 kb on + strand, within IAI47_12530 at 2639.925 kb on + strand, within IAI47_12530 at 2639.925 kb on + strand, within IAI47_12530 at 2639.925 kb on + strand, within IAI47_12530 at 2639.925 kb on + strand, within IAI47_12530 at 2639.925 kb on + strand, within IAI47_12530 at 2639.926 kb on - strand, within IAI47_12530 at 2639.926 kb on - strand, within IAI47_12530 at 2639.926 kb on - strand, within IAI47_12530 at 2639.958 kb on + strand, within IAI47_12530
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,637,649 + IAI47_12510 0.79 -1.0 2,637,649 + IAI47_12510 0.79 +0.5 2,637,649 + IAI47_12510 0.79 -0.3 2,637,649 + IAI47_12510 0.79 +0.7 2,637,649 + IAI47_12510 0.79 +0.1 2,637,649 + IAI47_12510 0.79 +0.4 2,637,650 - IAI47_12510 0.79 -0.3 2,637,683 + IAI47_12510 0.82 +0.1 2,637,683 + IAI47_12510 0.82 +1.6 2,637,683 + IAI47_12510 0.82 +0.3 2,637,684 - IAI47_12510 0.82 -0.0 2,637,721 + IAI47_12510 0.85 +0.6 2,637,751 + IAI47_12510 0.88 -0.0 2,637,751 + IAI47_12510 0.88 +0.4 2,637,752 - IAI47_12510 0.88 -0.6 2,637,752 - IAI47_12510 0.88 +0.6 2,637,752 - IAI47_12510 0.88 -1.3 2,637,752 - IAI47_12510 0.88 -0.9 2,637,800 + +0.1 2,637,800 + +1.2 2,637,845 + +0.9 2,637,846 - -1.6 2,637,846 - +0.4 2,637,893 + +0.4 2,637,904 + -0.2 2,637,991 + IAI47_12515 0.15 -0.1 2,637,992 - IAI47_12515 0.16 +0.5 2,638,004 + IAI47_12515 0.17 +2.1 2,638,005 - IAI47_12515 0.17 +0.9 2,638,022 + IAI47_12515 0.20 +1.4 2,638,023 - IAI47_12515 0.20 -0.4 2,638,151 + IAI47_12515 0.38 -0.5 2,638,151 + IAI47_12515 0.38 -0.9 2,638,151 + IAI47_12515 0.38 -0.1 2,638,151 + IAI47_12515 0.38 +1.5 2,638,151 + IAI47_12515 0.38 +0.3 2,638,152 - IAI47_12515 0.38 +0.5 2,638,152 - IAI47_12515 0.38 +1.6 2,638,152 - IAI47_12515 0.38 -3.6 2,638,152 - IAI47_12515 0.38 -0.1 2,638,180 + IAI47_12515 0.42 +0.7 2,638,204 - IAI47_12515 0.45 -2.0 2,638,337 + IAI47_12515 0.64 +0.9 2,638,337 + IAI47_12515 0.64 -2.0 2,638,337 + IAI47_12515 0.64 -1.6 2,638,338 - IAI47_12515 0.64 -1.4 2,638,338 - IAI47_12515 0.64 +1.9 2,638,338 - IAI47_12515 0.64 +3.6 2,638,338 - IAI47_12515 0.64 -0.1 2,638,338 - IAI47_12515 0.64 +2.1 2,638,340 - IAI47_12515 0.64 -1.1 2,638,431 + IAI47_12515 0.77 +1.1 2,638,454 + IAI47_12515 0.80 -0.7 2,638,454 + IAI47_12515 0.80 +0.6 2,638,454 + IAI47_12515 0.80 +0.1 2,638,455 - IAI47_12515 0.80 +0.9 2,638,484 + IAI47_12515 0.84 +1.0 2,638,484 + IAI47_12515 0.84 -0.3 2,638,484 + IAI47_12515 0.84 +0.4 2,638,484 + IAI47_12515 0.84 -0.4 2,638,484 + IAI47_12515 0.84 -1.7 2,638,484 + IAI47_12515 0.84 +0.8 2,638,485 - IAI47_12515 0.85 +0.5 2,638,564 + +3.2 2,638,565 - +1.0 2,638,601 - +0.0 2,638,601 - +2.0 2,638,614 + -0.0 2,638,614 + -0.1 2,638,614 + -1.1 2,638,615 - +0.5 2,638,615 - +0.6 2,638,615 - +0.7 2,638,626 + -0.2 2,638,626 + -0.6 2,638,626 + -0.1 2,638,627 - +0.1 2,638,627 - +1.1 2,638,627 - +1.0 2,638,627 - +2.3 2,638,657 - IAI47_12520 0.11 +1.6 2,638,657 - IAI47_12520 0.11 -1.1 2,638,657 - IAI47_12520 0.11 -0.3 2,638,657 - IAI47_12520 0.11 -1.6 2,638,699 + IAI47_12520 0.21 +0.1 2,638,699 + IAI47_12520 0.21 +0.9 2,638,699 + -0.1 2,638,699 + IAI47_12520 0.21 +0.9 2,638,700 - IAI47_12520 0.21 +0.6 2,638,700 - IAI47_12520 0.21 +0.4 2,638,700 - IAI47_12520 0.21 +0.7 2,638,700 - IAI47_12520 0.21 +0.2 2,638,700 - IAI47_12520 0.21 +0.1 2,638,710 + IAI47_12520 0.24 -0.7 2,638,711 - IAI47_12520 0.24 -0.0 2,638,711 - IAI47_12520 0.24 -0.1 2,638,711 - IAI47_12520 0.24 +1.0 2,638,711 - IAI47_12520 0.24 +2.2 2,638,711 - IAI47_12520 0.24 +0.2 2,638,791 + IAI47_12520 0.44 +3.9 2,638,791 + IAI47_12520 0.44 -1.5 2,638,839 + IAI47_12520 0.57 -0.7 2,638,870 + IAI47_12520 0.64 +1.7 2,638,871 - IAI47_12520 0.65 -0.5 2,638,871 - IAI47_12520 0.65 +1.0 2,638,891 + IAI47_12520 0.70 -0.6 2,638,900 + IAI47_12520 0.72 -0.9 2,638,900 + IAI47_12520 0.72 +0.4 2,638,901 - IAI47_12520 0.72 +0.0 2,639,142 + IAI47_12525 0.16 -0.4 2,639,142 + IAI47_12525 0.16 -0.1 2,639,143 - IAI47_12525 0.16 +1.9 2,639,143 - IAI47_12525 0.16 -0.3 2,639,143 - IAI47_12525 0.16 +0.2 2,639,190 + IAI47_12525 0.22 -2.1 2,639,190 + IAI47_12525 0.22 -1.2 2,639,190 + IAI47_12525 0.22 -1.0 2,639,338 + IAI47_12525 0.40 -0.1 2,639,338 + IAI47_12525 0.40 -0.5 2,639,339 - IAI47_12525 0.41 +0.4 2,639,339 - IAI47_12525 0.41 +0.5 2,639,339 - IAI47_12525 0.41 +1.7 2,639,343 - IAI47_12525 0.41 -0.4 2,639,343 - IAI47_12525 0.41 -0.4 2,639,343 - IAI47_12525 0.41 -0.6 2,639,389 + IAI47_12525 0.47 -0.1 2,639,390 - IAI47_12525 0.47 -0.4 2,639,505 - IAI47_12525 0.61 +1.2 2,639,817 + +1.0 2,639,817 + +1.0 2,639,838 + +0.7 2,639,838 + +0.8 2,639,838 + -0.9 2,639,838 + +1.4 2,639,838 + -2.0 2,639,839 - -0.6 2,639,839 - -0.1 2,639,839 - -0.7 2,639,839 - -0.7 2,639,856 + -0.8 2,639,922 + IAI47_12530 0.16 -0.2 2,639,922 + IAI47_12530 0.16 -0.5 2,639,922 + IAI47_12530 0.16 -1.9 2,639,922 + IAI47_12530 0.16 +0.6 2,639,922 + IAI47_12530 0.16 -0.3 2,639,922 + IAI47_12530 0.16 +0.4 2,639,922 + IAI47_12530 0.16 -0.6 2,639,923 - IAI47_12530 0.16 +1.1 2,639,923 - IAI47_12530 0.16 -1.0 2,639,923 - IAI47_12530 0.16 +1.2 2,639,923 - IAI47_12530 0.16 -0.4 2,639,925 + IAI47_12530 0.17 +0.2 2,639,925 + IAI47_12530 0.17 -2.0 2,639,925 + IAI47_12530 0.17 +0.3 2,639,925 + IAI47_12530 0.17 -0.6 2,639,925 + IAI47_12530 0.17 -0.7 2,639,925 + IAI47_12530 0.17 -0.7 2,639,925 + IAI47_12530 0.17 +2.4 2,639,925 + IAI47_12530 0.17 -0.3 2,639,925 + IAI47_12530 0.17 -0.2 2,639,925 + IAI47_12530 0.17 -0.7 2,639,926 - IAI47_12530 0.17 +0.5 2,639,926 - IAI47_12530 0.17 -0.8 2,639,926 - IAI47_12530 0.17 -0.8 2,639,958 + IAI47_12530 0.23 +0.3
Or see this region's nucleotide sequence