Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_21725 and IAI47_21730 are separated by 59 nucleotides IAI47_21730 and IAI47_21735 are separated by 83 nucleotides IAI47_21735 and IAI47_21740 are separated by 104 nucleotides IAI47_21740 and IAI47_21745 are separated by 14 nucleotides
IAI47_21725: IAI47_21725 - efflux RND transporter permease subunit, at 86,946 to 89,990
_21725
IAI47_21730: IAI47_21730 - hypothetical protein, at 90,050 to 90,295
_21730
IAI47_21735: IAI47_21735 - LysR family transcriptional regulator, at 90,379 to 91,272
_21735
IAI47_21740: IAI47_21740 - acetolactate decarboxylase, at 91,377 to 92,159
_21740
IAI47_21745: IAI47_21745 - acetolactate synthase AlsS, at 92,174 to 93,853
_21745
Position (kb) 90
91
92 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 89.468 kb on + strand, within IAI47_21725 at 89.468 kb on + strand, within IAI47_21725 at 89.468 kb on + strand, within IAI47_21725 at 89.468 kb on + strand, within IAI47_21725 at 89.469 kb on - strand, within IAI47_21725 at 89.469 kb on - strand, within IAI47_21725 at 89.469 kb on - strand, within IAI47_21725 at 89.469 kb on - strand, within IAI47_21725 at 89.638 kb on + strand, within IAI47_21725 at 89.639 kb on - strand, within IAI47_21725 at 89.639 kb on - strand, within IAI47_21725 at 89.639 kb on - strand, within IAI47_21725 at 89.660 kb on + strand, within IAI47_21725 at 89.693 kb on + strand at 89.694 kb on - strand at 89.732 kb on + strand at 89.732 kb on + strand at 89.732 kb on + strand at 89.732 kb on + strand at 89.733 kb on - strand at 89.733 kb on - strand at 89.750 kb on + strand at 89.786 kb on + strand at 89.861 kb on + strand at 89.862 kb on - strand at 89.862 kb on - strand at 89.904 kb on - strand at 89.925 kb on - strand at 89.939 kb on + strand at 89.939 kb on + strand at 89.939 kb on + strand at 89.939 kb on + strand at 89.939 kb on + strand at 89.939 kb on + strand at 89.939 kb on + strand at 89.940 kb on - strand at 89.940 kb on - strand at 89.940 kb on - strand at 89.940 kb on - strand at 89.940 kb on - strand at 89.940 kb on - strand at 89.944 kb on + strand at 89.960 kb on - strand at 89.988 kb on + strand at 89.989 kb on - strand at 90.108 kb on + strand, within IAI47_21730 at 90.108 kb on + strand, within IAI47_21730 at 90.108 kb on + strand, within IAI47_21730 at 90.108 kb on + strand, within IAI47_21730 at 90.109 kb on - strand, within IAI47_21730 at 90.109 kb on - strand, within IAI47_21730 at 90.153 kb on + strand, within IAI47_21730 at 90.154 kb on - strand, within IAI47_21730 at 90.154 kb on - strand, within IAI47_21730 at 90.154 kb on - strand, within IAI47_21730 at 90.159 kb on + strand, within IAI47_21730 at 90.159 kb on + strand, within IAI47_21730 at 90.159 kb on + strand, within IAI47_21730 at 90.159 kb on + strand, within IAI47_21730 at 90.159 kb on + strand, within IAI47_21730 at 90.160 kb on - strand, within IAI47_21730 at 90.160 kb on - strand, within IAI47_21730 at 90.160 kb on - strand, within IAI47_21730 at 90.171 kb on + strand, within IAI47_21730 at 90.172 kb on - strand, within IAI47_21730 at 90.278 kb on + strand at 90.278 kb on + strand at 90.278 kb on + strand at 90.279 kb on - strand at 90.279 kb on - strand at 90.285 kb on + strand at 90.285 kb on + strand at 90.286 kb on - strand at 90.286 kb on - strand at 90.286 kb on - strand at 90.287 kb on + strand at 90.287 kb on + strand at 90.287 kb on + strand at 90.287 kb on + strand at 90.287 kb on + strand at 90.287 kb on + strand at 90.287 kb on + strand at 90.287 kb on + strand at 90.287 kb on + strand at 90.287 kb on + strand at 90.287 kb on + strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.288 kb on - strand at 90.289 kb on + strand at 90.289 kb on + strand at 90.289 kb on + strand at 90.289 kb on + strand at 90.289 kb on + strand at 90.289 kb on + strand at 90.289 kb on + strand at 90.289 kb on + strand at 90.289 kb on + strand at 90.290 kb on - strand at 90.290 kb on - strand at 90.290 kb on - strand at 90.290 kb on - strand at 90.290 kb on - strand at 90.290 kb on - strand at 90.290 kb on - strand at 90.290 kb on - strand at 90.290 kb on - strand at 90.290 kb on - strand at 90.290 kb on - strand at 90.290 kb on - strand at 90.305 kb on - strand at 90.337 kb on + strand at 90.338 kb on - strand at 90.350 kb on - strand at 90.350 kb on - strand at 90.354 kb on + strand at 90.354 kb on + strand at 90.354 kb on + strand at 90.354 kb on + strand at 90.354 kb on + strand at 90.354 kb on + strand at 90.354 kb on + strand at 90.354 kb on + strand at 90.355 kb on - strand at 90.355 kb on - strand at 90.355 kb on - strand at 90.380 kb on + strand at 90.380 kb on + strand at 90.380 kb on + strand at 90.381 kb on - strand at 90.381 kb on - strand at 90.574 kb on - strand, within IAI47_21735 at 90.659 kb on + strand, within IAI47_21735 at 90.659 kb on + strand, within IAI47_21735 at 90.660 kb on - strand, within IAI47_21735 at 90.660 kb on - strand, within IAI47_21735 at 90.660 kb on - strand, within IAI47_21735 at 90.660 kb on - strand, within IAI47_21735 at 90.660 kb on - strand, within IAI47_21735 at 90.735 kb on + strand, within IAI47_21735 at 90.736 kb on - strand, within IAI47_21735 at 90.742 kb on + strand, within IAI47_21735 at 90.743 kb on - strand, within IAI47_21735 at 90.851 kb on - strand, within IAI47_21735 at 90.862 kb on + strand, within IAI47_21735 at 90.862 kb on + strand, within IAI47_21735 at 90.862 kb on + strand, within IAI47_21735 at 90.862 kb on + strand, within IAI47_21735 at 90.885 kb on + strand, within IAI47_21735 at 90.885 kb on + strand, within IAI47_21735 at 90.885 kb on + strand, within IAI47_21735 at 90.885 kb on + strand, within IAI47_21735 at 90.885 kb on + strand, within IAI47_21735 at 90.885 kb on + strand, within IAI47_21735 at 90.885 kb on + strand, within IAI47_21735 at 90.886 kb on - strand, within IAI47_21735 at 90.920 kb on + strand, within IAI47_21735 at 90.931 kb on - strand, within IAI47_21735 at 90.936 kb on + strand, within IAI47_21735 at 90.937 kb on - strand, within IAI47_21735 at 90.937 kb on - strand, within IAI47_21735 at 90.937 kb on - strand, within IAI47_21735 at 90.937 kb on - strand, within IAI47_21735 at 90.963 kb on + strand, within IAI47_21735 at 90.963 kb on + strand, within IAI47_21735 at 90.964 kb on - strand, within IAI47_21735 at 91.165 kb on + strand, within IAI47_21735 at 91.165 kb on + strand, within IAI47_21735 at 91.165 kb on + strand, within IAI47_21735 at 91.166 kb on - strand, within IAI47_21735 at 91.193 kb on - strand at 91.251 kb on - strand at 91.272 kb on + strand at 91.272 kb on + strand at 91.273 kb on - strand at 91.273 kb on - strand at 91.293 kb on - strand at 91.305 kb on + strand at 91.359 kb on + strand at 91.418 kb on - strand at 91.418 kb on - strand at 91.418 kb on - strand at 91.424 kb on + strand at 91.424 kb on + strand at 91.425 kb on - strand at 91.425 kb on - strand at 91.425 kb on - strand at 91.425 kb on - strand at 91.432 kb on + strand at 91.432 kb on + strand at 91.433 kb on - strand at 91.463 kb on + strand, within IAI47_21740 at 91.463 kb on + strand, within IAI47_21740 at 91.464 kb on - strand, within IAI47_21740 at 91.464 kb on - strand, within IAI47_21740 at 91.467 kb on + strand, within IAI47_21740 at 91.468 kb on - strand, within IAI47_21740 at 91.468 kb on - strand, within IAI47_21740 at 91.468 kb on - strand, within IAI47_21740 at 91.506 kb on + strand, within IAI47_21740 at 91.507 kb on - strand, within IAI47_21740 at 91.507 kb on - strand, within IAI47_21740 at 91.514 kb on + strand, within IAI47_21740 at 91.515 kb on - strand, within IAI47_21740 at 91.611 kb on + strand, within IAI47_21740 at 91.611 kb on + strand, within IAI47_21740 at 91.611 kb on + strand, within IAI47_21740 at 91.612 kb on - strand, within IAI47_21740 at 91.790 kb on - strand, within IAI47_21740 at 91.790 kb on - strand, within IAI47_21740 at 91.886 kb on + strand, within IAI47_21740 at 91.886 kb on + strand, within IAI47_21740 at 91.886 kb on + strand, within IAI47_21740 at 91.887 kb on - strand, within IAI47_21740 at 91.887 kb on - strand, within IAI47_21740 at 91.941 kb on + strand, within IAI47_21740 at 91.941 kb on + strand, within IAI47_21740 at 91.941 kb on + strand, within IAI47_21740 at 91.941 kb on + strand, within IAI47_21740 at 91.941 kb on + strand, within IAI47_21740 at 91.941 kb on + strand, within IAI47_21740 at 91.941 kb on + strand, within IAI47_21740 at 91.941 kb on + strand, within IAI47_21740 at 91.942 kb on - strand, within IAI47_21740 at 91.942 kb on - strand, within IAI47_21740 at 91.942 kb on - strand, within IAI47_21740 at 91.942 kb on - strand, within IAI47_21740 at 91.942 kb on - strand, within IAI47_21740 at 91.942 kb on - strand, within IAI47_21740 at 91.943 kb on + strand, within IAI47_21740 at 91.943 kb on + strand, within IAI47_21740 at 91.943 kb on + strand, within IAI47_21740 at 91.943 kb on + strand, within IAI47_21740 at 91.943 kb on + strand, within IAI47_21740 at 91.943 kb on + strand, within IAI47_21740 at 91.943 kb on + strand, within IAI47_21740 at 91.943 kb on + strand, within IAI47_21740 at 91.943 kb on + strand, within IAI47_21740 at 91.943 kb on + strand, within IAI47_21740 at 91.944 kb on - strand, within IAI47_21740 at 91.944 kb on - strand, within IAI47_21740 at 91.944 kb on - strand, within IAI47_21740 at 91.944 kb on - strand, within IAI47_21740 at 91.944 kb on - strand, within IAI47_21740 at 91.944 kb on - strand, within IAI47_21740 at 91.944 kb on - strand, within IAI47_21740 at 91.944 kb on - strand, within IAI47_21740 at 91.944 kb on - strand, within IAI47_21740 at 91.944 kb on - strand, within IAI47_21740 at 91.944 kb on - strand, within IAI47_21740 at 91.944 kb on - strand, within IAI47_21740 at 91.944 kb on - strand, within IAI47_21740 at 91.944 kb on - strand, within IAI47_21740 at 91.955 kb on + strand, within IAI47_21740 at 91.956 kb on - strand, within IAI47_21740 at 91.982 kb on + strand, within IAI47_21740 at 91.983 kb on - strand, within IAI47_21740 at 91.983 kb on - strand, within IAI47_21740 at 91.986 kb on - strand, within IAI47_21740 at 92.150 kb on - strand at 92.157 kb on + strand at 92.157 kb on + strand at 92.157 kb on + strand at 92.157 kb on + strand at 92.157 kb on + strand at 92.157 kb on + strand at 92.158 kb on - strand at 92.191 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 89,468 + IAI47_21725 0.83 -0.0 89,468 + IAI47_21725 0.83 +1.3 89,468 + IAI47_21725 0.83 +1.6 89,468 + IAI47_21725 0.83 +0.6 89,469 - IAI47_21725 0.83 -0.6 89,469 - IAI47_21725 0.83 +0.6 89,469 - IAI47_21725 0.83 +1.5 89,469 - IAI47_21725 0.83 -1.6 89,638 + IAI47_21725 0.88 -1.2 89,639 - IAI47_21725 0.88 +2.2 89,639 - IAI47_21725 0.88 +0.6 89,639 - IAI47_21725 0.88 -0.5 89,660 + IAI47_21725 0.89 +0.7 89,693 + -0.1 89,694 - +1.0 89,732 + +0.3 89,732 + -3.5 89,732 + +0.4 89,732 + +1.0 89,733 - +1.0 89,733 - +0.5 89,750 + -1.1 89,786 + +0.6 89,861 + +0.0 89,862 - -1.9 89,862 - +1.1 89,904 - +0.8 89,925 - -0.4 89,939 + -0.8 89,939 + -1.6 89,939 + +2.2 89,939 + -0.7 89,939 + +1.0 89,939 + -0.5 89,939 + -0.9 89,940 - +0.4 89,940 - +0.1 89,940 - +0.7 89,940 - +0.6 89,940 - -0.4 89,940 - +0.5 89,944 + +2.2 89,960 - +0.5 89,988 + +1.6 89,989 - +2.4 90,108 + IAI47_21730 0.24 +0.2 90,108 + IAI47_21730 0.24 -0.8 90,108 + IAI47_21730 0.24 +2.6 90,108 + IAI47_21730 0.24 -1.5 90,109 - IAI47_21730 0.24 +0.8 90,109 - IAI47_21730 0.24 +1.6 90,153 + IAI47_21730 0.42 -1.0 90,154 - IAI47_21730 0.42 +0.7 90,154 - IAI47_21730 0.42 +0.8 90,154 - IAI47_21730 0.42 +0.9 90,159 + IAI47_21730 0.44 -0.1 90,159 + IAI47_21730 0.44 -1.5 90,159 + IAI47_21730 0.44 +0.0 90,159 + IAI47_21730 0.44 -0.0 90,159 + IAI47_21730 0.44 -1.7 90,160 - IAI47_21730 0.45 +1.4 90,160 - IAI47_21730 0.45 -0.6 90,160 - IAI47_21730 0.45 -1.6 90,171 + IAI47_21730 0.49 -0.5 90,172 - IAI47_21730 0.50 -2.1 90,278 + -1.6 90,278 + -0.7 90,278 + -2.3 90,279 - +0.8 90,279 - -0.2 90,285 + -1.1 90,285 + -0.5 90,286 - +0.2 90,286 - -2.1 90,286 - -1.1 90,287 + +0.2 90,287 + +1.3 90,287 + +0.3 90,287 + +1.0 90,287 + +0.6 90,287 + -0.5 90,287 + +2.2 90,287 + -1.0 90,287 + -2.8 90,287 + +0.7 90,287 + +0.2 90,288 - -0.6 90,288 - +1.2 90,288 - -0.3 90,288 - -0.9 90,288 - +0.1 90,288 - -0.6 90,288 - +1.1 90,288 - -1.6 90,288 - -0.3 90,288 - +2.6 90,288 - +2.0 90,288 - +0.3 90,288 - -0.1 90,288 - +2.2 90,288 - +1.2 90,288 - -0.6 90,288 - +1.1 90,288 - +1.0 90,288 - -0.1 90,288 - +0.5 90,288 - +0.1 90,288 - +0.1 90,288 - -0.4 90,289 + +1.6 90,289 + -0.9 90,289 + +0.2 90,289 + -0.3 90,289 + -0.4 90,289 + +0.5 90,289 + +1.8 90,289 + -0.1 90,289 + +2.2 90,290 - +0.4 90,290 - -0.8 90,290 - -0.3 90,290 - +0.5 90,290 - +1.4 90,290 - -0.3 90,290 - -0.2 90,290 - -1.6 90,290 - +0.6 90,290 - +0.6 90,290 - +0.3 90,290 - +0.7 90,305 - -0.2 90,337 + +0.4 90,338 - +0.2 90,350 - -1.2 90,350 - -0.1 90,354 + -0.0 90,354 + +1.2 90,354 + +0.1 90,354 + -0.0 90,354 + +0.8 90,354 + -0.1 90,354 + +0.9 90,354 + -2.6 90,355 - -0.0 90,355 - +0.3 90,355 - -0.1 90,380 + -0.1 90,380 + -1.6 90,380 + -3.2 90,381 - -0.8 90,381 - -0.5 90,574 - IAI47_21735 0.22 +0.5 90,659 + IAI47_21735 0.31 -0.8 90,659 + IAI47_21735 0.31 -1.1 90,660 - IAI47_21735 0.31 -0.1 90,660 - IAI47_21735 0.31 +0.6 90,660 - IAI47_21735 0.31 +0.1 90,660 - IAI47_21735 0.31 -2.0 90,660 - IAI47_21735 0.31 +0.6 90,735 + IAI47_21735 0.40 -0.1 90,736 - IAI47_21735 0.40 +0.0 90,742 + IAI47_21735 0.41 -0.1 90,743 - IAI47_21735 0.41 +0.8 90,851 - IAI47_21735 0.53 +0.6 90,862 + IAI47_21735 0.54 -1.0 90,862 + IAI47_21735 0.54 -0.3 90,862 + IAI47_21735 0.54 -0.9 90,862 + IAI47_21735 0.54 -1.1 90,885 + IAI47_21735 0.57 +0.9 90,885 + IAI47_21735 0.57 -1.0 90,885 + IAI47_21735 0.57 -1.0 90,885 + IAI47_21735 0.57 -1.3 90,885 + IAI47_21735 0.57 -1.1 90,885 + IAI47_21735 0.57 +2.2 90,885 + IAI47_21735 0.57 -2.0 90,886 - IAI47_21735 0.57 +0.0 90,920 + IAI47_21735 0.61 -1.5 90,931 - IAI47_21735 0.62 -1.5 90,936 + IAI47_21735 0.62 +1.0 90,937 - IAI47_21735 0.62 +0.5 90,937 - IAI47_21735 0.62 +1.0 90,937 - IAI47_21735 0.62 +0.5 90,937 - IAI47_21735 0.62 -0.2 90,963 + IAI47_21735 0.65 +1.3 90,963 + IAI47_21735 0.65 +0.6 90,964 - IAI47_21735 0.65 -0.3 91,165 + IAI47_21735 0.88 +0.8 91,165 + IAI47_21735 0.88 -1.0 91,165 + IAI47_21735 0.88 -0.5 91,166 - IAI47_21735 0.88 -1.1 91,193 - +0.0 91,251 - +0.5 91,272 + +0.9 91,272 + -0.1 91,273 - +1.1 91,273 - -2.2 91,293 - +1.9 91,305 + +0.8 91,359 + +0.4 91,418 - +0.2 91,418 - +0.2 91,418 - +1.4 91,424 + +0.6 91,424 + -1.4 91,425 - -1.7 91,425 - +1.8 91,425 - -0.4 91,425 - -0.2 91,432 + -0.7 91,432 + +1.8 91,433 - +0.3 91,463 + IAI47_21740 0.11 -0.6 91,463 + IAI47_21740 0.11 +1.0 91,464 - IAI47_21740 0.11 +1.0 91,464 - IAI47_21740 0.11 +0.6 91,467 + IAI47_21740 0.11 -0.2 91,468 - IAI47_21740 0.12 +1.3 91,468 - IAI47_21740 0.12 +0.6 91,468 - IAI47_21740 0.12 -0.1 91,506 + IAI47_21740 0.16 +0.8 91,507 - IAI47_21740 0.17 -0.4 91,507 - IAI47_21740 0.17 -0.2 91,514 + IAI47_21740 0.17 -0.6 91,515 - IAI47_21740 0.18 +0.9 91,611 + IAI47_21740 0.30 +1.0 91,611 + IAI47_21740 0.30 -1.0 91,611 + IAI47_21740 0.30 -0.0 91,612 - IAI47_21740 0.30 -1.5 91,790 - IAI47_21740 0.53 -1.8 91,790 - IAI47_21740 0.53 -0.5 91,886 + IAI47_21740 0.65 +0.7 91,886 + IAI47_21740 0.65 -1.2 91,886 + IAI47_21740 0.65 -0.2 91,887 - IAI47_21740 0.65 +0.6 91,887 - IAI47_21740 0.65 +0.4 91,941 + IAI47_21740 0.72 +0.6 91,941 + IAI47_21740 0.72 +0.8 91,941 + IAI47_21740 0.72 +0.3 91,941 + IAI47_21740 0.72 +0.1 91,941 + IAI47_21740 0.72 +1.3 91,941 + IAI47_21740 0.72 -0.1 91,941 + IAI47_21740 0.72 +1.1 91,941 + IAI47_21740 0.72 +0.6 91,942 - IAI47_21740 0.72 +1.2 91,942 - IAI47_21740 0.72 -0.2 91,942 - IAI47_21740 0.72 +1.0 91,942 - IAI47_21740 0.72 +0.3 91,942 - IAI47_21740 0.72 +1.0 91,942 - IAI47_21740 0.72 -1.5 91,943 + IAI47_21740 0.72 +1.1 91,943 + IAI47_21740 0.72 -0.8 91,943 + IAI47_21740 0.72 -0.4 91,943 + IAI47_21740 0.72 +0.0 91,943 + IAI47_21740 0.72 -2.1 91,943 + IAI47_21740 0.72 -0.1 91,943 + IAI47_21740 0.72 +0.5 91,943 + IAI47_21740 0.72 +0.6 91,943 + IAI47_21740 0.72 +1.8 91,943 + IAI47_21740 0.72 -1.0 91,944 - IAI47_21740 0.72 -0.6 91,944 - IAI47_21740 0.72 -0.7 91,944 - IAI47_21740 0.72 -0.1 91,944 - IAI47_21740 0.72 +0.0 91,944 - IAI47_21740 0.72 -0.6 91,944 - IAI47_21740 0.72 +0.3 91,944 - IAI47_21740 0.72 +0.3 91,944 - IAI47_21740 0.72 +0.1 91,944 - IAI47_21740 0.72 +1.0 91,944 - IAI47_21740 0.72 -0.7 91,944 - IAI47_21740 0.72 -0.1 91,944 - IAI47_21740 0.72 +0.9 91,944 - IAI47_21740 0.72 +0.0 91,944 - IAI47_21740 0.72 -0.5 91,955 + IAI47_21740 0.74 -0.5 91,956 - IAI47_21740 0.74 -0.3 91,982 + IAI47_21740 0.77 -0.5 91,983 - IAI47_21740 0.77 -0.5 91,983 - IAI47_21740 0.77 -0.0 91,986 - IAI47_21740 0.78 +0.9 92,150 - -2.3 92,157 + -0.8 92,157 + -0.4 92,157 + -0.4 92,157 + -1.3 92,157 + -0.5 92,157 + +1.4 92,158 - +1.1 92,191 + +1.4
Or see this region's nucleotide sequence