Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_03195 and IAI47_03200 are separated by 99 nucleotides IAI47_03200 and IAI47_03205 are separated by 204 nucleotides
IAI47_03195: IAI47_03195 - cell division protein FtsP, at 662,188 to 663,618
_03195
IAI47_03200: IAI47_03200 - 2,5-didehydrogluconate reductase DkgA, at 663,718 to 664,542
_03200
IAI47_03205: IAI47_03205 - AraC family transcriptional regulator, at 664,747 to 665,655
_03205
Position (kb) 663
664
665 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 662.852 kb on + strand, within IAI47_03195 at 662.939 kb on + strand, within IAI47_03195 at 662.939 kb on + strand, within IAI47_03195 at 663.050 kb on + strand, within IAI47_03195 at 663.054 kb on + strand, within IAI47_03195 at 663.147 kb on + strand, within IAI47_03195 at 663.148 kb on - strand, within IAI47_03195 at 663.154 kb on - strand, within IAI47_03195 at 663.185 kb on + strand, within IAI47_03195 at 663.551 kb on - strand at 663.616 kb on + strand at 663.616 kb on + strand at 663.616 kb on + strand at 663.616 kb on + strand at 663.657 kb on - strand at 663.657 kb on - strand at 663.660 kb on + strand at 663.660 kb on + strand at 663.660 kb on + strand at 663.661 kb on - strand at 663.661 kb on - strand at 663.661 kb on - strand at 663.667 kb on + strand at 663.668 kb on - strand at 663.668 kb on - strand at 663.668 kb on - strand at 663.668 kb on - strand at 663.668 kb on - strand at 663.692 kb on + strand at 663.692 kb on + strand at 663.692 kb on + strand at 663.692 kb on + strand at 663.693 kb on - strand at 663.719 kb on + strand at 663.719 kb on + strand at 663.719 kb on + strand at 663.719 kb on + strand at 663.719 kb on + strand at 663.720 kb on - strand at 663.720 kb on - strand at 663.720 kb on - strand at 663.720 kb on - strand at 663.720 kb on - strand at 663.723 kb on + strand at 663.723 kb on + strand at 663.723 kb on + strand at 663.723 kb on + strand at 663.723 kb on + strand at 663.724 kb on - strand at 663.724 kb on - strand at 663.774 kb on + strand at 663.774 kb on + strand at 663.775 kb on - strand at 663.792 kb on + strand at 663.793 kb on - strand at 663.816 kb on + strand, within IAI47_03200 at 663.816 kb on + strand, within IAI47_03200 at 663.816 kb on + strand, within IAI47_03200 at 663.817 kb on - strand, within IAI47_03200 at 663.817 kb on - strand, within IAI47_03200 at 663.817 kb on - strand, within IAI47_03200 at 663.918 kb on - strand, within IAI47_03200 at 663.918 kb on - strand, within IAI47_03200 at 663.918 kb on - strand, within IAI47_03200 at 663.918 kb on - strand, within IAI47_03200 at 663.918 kb on - strand, within IAI47_03200 at 663.936 kb on + strand, within IAI47_03200 at 663.936 kb on + strand, within IAI47_03200 at 663.936 kb on + strand, within IAI47_03200 at 663.936 kb on + strand, within IAI47_03200 at 663.936 kb on + strand, within IAI47_03200 at 663.937 kb on - strand, within IAI47_03200 at 663.937 kb on - strand, within IAI47_03200 at 663.937 kb on - strand, within IAI47_03200 at 663.937 kb on - strand, within IAI47_03200 at 664.032 kb on + strand, within IAI47_03200 at 664.032 kb on + strand, within IAI47_03200 at 664.033 kb on - strand, within IAI47_03200 at 664.069 kb on + strand, within IAI47_03200 at 664.070 kb on - strand, within IAI47_03200 at 664.128 kb on + strand, within IAI47_03200 at 664.128 kb on + strand, within IAI47_03200 at 664.129 kb on - strand, within IAI47_03200 at 664.129 kb on - strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.196 kb on + strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.197 kb on - strand, within IAI47_03200 at 664.232 kb on + strand, within IAI47_03200 at 664.232 kb on + strand, within IAI47_03200 at 664.232 kb on + strand, within IAI47_03200 at 664.233 kb on - strand, within IAI47_03200 at 664.391 kb on + strand, within IAI47_03200 at 664.392 kb on - strand, within IAI47_03200 at 664.392 kb on - strand, within IAI47_03200 at 664.404 kb on + strand, within IAI47_03200 at 664.419 kb on - strand, within IAI47_03200 at 664.477 kb on + strand at 664.478 kb on - strand at 664.478 kb on - strand at 664.503 kb on + strand at 664.503 kb on + strand at 664.503 kb on + strand at 664.503 kb on + strand at 664.503 kb on + strand at 664.504 kb on - strand at 664.504 kb on - strand at 664.504 kb on - strand at 664.504 kb on - strand at 664.504 kb on - strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.542 kb on + strand at 664.543 kb on - strand at 664.543 kb on - strand at 664.543 kb on - strand at 664.543 kb on - strand at 664.589 kb on + strand at 664.589 kb on + strand at 664.589 kb on + strand at 664.589 kb on + strand at 664.589 kb on + strand at 664.589 kb on + strand at 664.590 kb on - strand at 664.590 kb on - strand at 664.590 kb on - strand at 664.590 kb on - strand at 664.596 kb on - strand at 664.596 kb on - strand at 664.618 kb on + strand at 664.618 kb on + strand at 664.618 kb on + strand at 664.618 kb on + strand at 664.619 kb on - strand at 664.619 kb on - strand at 664.619 kb on - strand at 664.619 kb on - strand at 664.619 kb on - strand at 664.619 kb on - strand at 664.630 kb on + strand at 664.648 kb on + strand at 664.649 kb on - strand at 664.649 kb on - strand at 664.649 kb on - strand at 664.649 kb on - strand at 664.649 kb on - strand at 664.663 kb on - strand at 664.668 kb on + strand at 664.669 kb on - strand at 664.746 kb on + strand at 664.746 kb on + strand at 664.797 kb on - strand at 664.797 kb on - strand at 664.850 kb on - strand, within IAI47_03205 at 664.885 kb on + strand, within IAI47_03205 at 664.885 kb on + strand, within IAI47_03205 at 664.885 kb on + strand, within IAI47_03205 at 664.886 kb on - strand, within IAI47_03205 at 664.886 kb on - strand, within IAI47_03205 at 664.886 kb on - strand, within IAI47_03205 at 664.886 kb on - strand, within IAI47_03205 at 664.886 kb on - strand, within IAI47_03205 at 664.886 kb on - strand, within IAI47_03205 at 664.886 kb on - strand, within IAI47_03205 at 664.886 kb on - strand, within IAI47_03205 at 664.931 kb on - strand, within IAI47_03205 at 664.931 kb on - strand, within IAI47_03205 at 664.954 kb on + strand, within IAI47_03205 at 664.954 kb on + strand, within IAI47_03205 at 664.955 kb on - strand, within IAI47_03205 at 664.955 kb on - strand, within IAI47_03205 at 664.969 kb on + strand, within IAI47_03205 at 664.969 kb on + strand, within IAI47_03205 at 664.969 kb on + strand, within IAI47_03205 at 664.970 kb on - strand, within IAI47_03205 at 665.027 kb on + strand, within IAI47_03205 at 665.028 kb on - strand, within IAI47_03205 at 665.247 kb on + strand, within IAI47_03205 at 665.247 kb on + strand, within IAI47_03205 at 665.248 kb on - strand, within IAI47_03205 at 665.248 kb on - strand, within IAI47_03205 at 665.248 kb on - strand, within IAI47_03205 at 665.251 kb on + strand, within IAI47_03205 at 665.251 kb on + strand, within IAI47_03205 at 665.251 kb on + strand, within IAI47_03205 at 665.251 kb on + strand, within IAI47_03205 at 665.252 kb on - strand, within IAI47_03205 at 665.252 kb on - strand, within IAI47_03205 at 665.252 kb on - strand, within IAI47_03205 at 665.252 kb on - strand, within IAI47_03205 at 665.252 kb on - strand, within IAI47_03205 at 665.252 kb on - strand, within IAI47_03205 at 665.252 kb on - strand, within IAI47_03205 at 665.252 kb on - strand, within IAI47_03205 at 665.252 kb on - strand, within IAI47_03205 at 665.252 kb on - strand, within IAI47_03205 at 665.254 kb on + strand, within IAI47_03205 at 665.254 kb on + strand, within IAI47_03205 at 665.254 kb on + strand, within IAI47_03205 at 665.254 kb on + strand, within IAI47_03205 at 665.254 kb on + strand, within IAI47_03205 at 665.254 kb on + strand, within IAI47_03205 at 665.254 kb on + strand, within IAI47_03205 at 665.254 kb on + strand, within IAI47_03205 at 665.255 kb on - strand, within IAI47_03205 at 665.255 kb on - strand, within IAI47_03205 at 665.255 kb on - strand, within IAI47_03205 at 665.255 kb on - strand, within IAI47_03205 at 665.255 kb on - strand, within IAI47_03205 at 665.255 kb on - strand, within IAI47_03205 at 665.255 kb on - strand, within IAI47_03205 at 665.255 kb on - strand, within IAI47_03205 at 665.255 kb on - strand, within IAI47_03205 at 665.255 kb on - strand, within IAI47_03205 at 665.255 kb on - strand, within IAI47_03205 at 665.255 kb on - strand, within IAI47_03205 at 665.255 kb on - strand, within IAI47_03205 at 665.255 kb on - strand, within IAI47_03205 at 665.356 kb on + strand, within IAI47_03205 at 665.357 kb on - strand, within IAI47_03205 at 665.357 kb on - strand, within IAI47_03205 at 665.424 kb on + strand, within IAI47_03205 at 665.425 kb on - strand, within IAI47_03205 at 665.456 kb on + strand, within IAI47_03205 at 665.456 kb on + strand, within IAI47_03205 at 665.456 kb on + strand, within IAI47_03205 at 665.456 kb on + strand, within IAI47_03205 at 665.456 kb on + strand, within IAI47_03205 at 665.456 kb on + strand, within IAI47_03205 at 665.456 kb on + strand, within IAI47_03205 at 665.457 kb on - strand, within IAI47_03205 at 665.457 kb on - strand, within IAI47_03205 at 665.457 kb on - strand, within IAI47_03205 at 665.457 kb on - strand, within IAI47_03205 at 665.461 kb on + strand, within IAI47_03205 at 665.461 kb on + strand, within IAI47_03205 at 665.461 kb on + strand, within IAI47_03205 at 665.461 kb on + strand, within IAI47_03205 at 665.461 kb on + strand, within IAI47_03205 at 665.461 kb on + strand, within IAI47_03205 at 665.462 kb on - strand, within IAI47_03205 at 665.462 kb on - strand, within IAI47_03205 at 665.462 kb on - strand, within IAI47_03205 at 665.462 kb on - strand, within IAI47_03205 at 665.462 kb on - strand, within IAI47_03205 at 665.462 kb on - strand, within IAI47_03205
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 662,852 + IAI47_03195 0.46 +0.0 662,939 + IAI47_03195 0.52 -0.2 662,939 + IAI47_03195 0.52 +0.1 663,050 + IAI47_03195 0.60 +1.5 663,054 + IAI47_03195 0.61 -0.4 663,147 + IAI47_03195 0.67 +0.0 663,148 - IAI47_03195 0.67 -1.1 663,154 - IAI47_03195 0.68 -1.0 663,185 + IAI47_03195 0.70 -2.6 663,551 - -1.6 663,616 + -0.8 663,616 + +1.1 663,616 + +0.4 663,616 + +0.1 663,657 - +0.6 663,657 - -0.5 663,660 + -0.8 663,660 + -0.5 663,660 + +0.5 663,661 - +1.3 663,661 - +0.2 663,661 - -1.4 663,667 + -1.1 663,668 - +3.3 663,668 - -0.5 663,668 - -1.2 663,668 - -1.3 663,668 - -0.8 663,692 + +0.3 663,692 + -0.9 663,692 + +0.9 663,692 + +4.0 663,693 - +0.0 663,719 + +1.4 663,719 + -1.0 663,719 + -0.3 663,719 + -1.2 663,719 + -0.2 663,720 - +1.2 663,720 - -0.2 663,720 - -0.3 663,720 - -0.3 663,720 - +0.9 663,723 + -0.8 663,723 + -0.7 663,723 + +0.1 663,723 + -0.6 663,723 + -0.7 663,724 - +0.5 663,724 - -0.2 663,774 + -0.2 663,774 + -0.1 663,775 - +2.6 663,792 + -0.1 663,793 - +0.1 663,816 + IAI47_03200 0.12 +0.3 663,816 + IAI47_03200 0.12 +0.1 663,816 + IAI47_03200 0.12 +1.5 663,817 - IAI47_03200 0.12 +0.2 663,817 - IAI47_03200 0.12 -0.3 663,817 - IAI47_03200 0.12 +1.6 663,918 - IAI47_03200 0.24 -1.5 663,918 - IAI47_03200 0.24 +0.4 663,918 - IAI47_03200 0.24 +2.1 663,918 - IAI47_03200 0.24 +1.0 663,918 - IAI47_03200 0.24 -0.7 663,936 + IAI47_03200 0.26 -1.8 663,936 + IAI47_03200 0.26 -0.9 663,936 + IAI47_03200 0.26 -0.7 663,936 + IAI47_03200 0.26 -1.0 663,936 + IAI47_03200 0.26 +1.2 663,937 - IAI47_03200 0.27 -0.8 663,937 - IAI47_03200 0.27 -0.5 663,937 - IAI47_03200 0.27 +0.8 663,937 - IAI47_03200 0.27 -2.0 664,032 + IAI47_03200 0.38 -0.3 664,032 + IAI47_03200 0.38 +1.3 664,033 - IAI47_03200 0.38 -1.2 664,069 + IAI47_03200 0.43 +0.3 664,070 - IAI47_03200 0.43 +0.7 664,128 + IAI47_03200 0.50 -0.4 664,128 + IAI47_03200 0.50 -0.4 664,129 - IAI47_03200 0.50 -2.1 664,129 - IAI47_03200 0.50 +0.8 664,196 + IAI47_03200 0.58 -0.5 664,196 + IAI47_03200 0.58 -0.3 664,196 + IAI47_03200 0.58 +0.9 664,196 + IAI47_03200 0.58 +0.1 664,196 + IAI47_03200 0.58 +0.9 664,196 + IAI47_03200 0.58 -1.1 664,196 + IAI47_03200 0.58 +1.1 664,196 + IAI47_03200 0.58 -0.5 664,197 - IAI47_03200 0.58 +2.4 664,197 - IAI47_03200 0.58 +0.5 664,197 - IAI47_03200 0.58 +0.7 664,197 - IAI47_03200 0.58 -0.8 664,197 - IAI47_03200 0.58 -0.7 664,197 - IAI47_03200 0.58 +0.4 664,197 - IAI47_03200 0.58 +0.3 664,197 - IAI47_03200 0.58 +0.1 664,197 - IAI47_03200 0.58 -0.4 664,197 - IAI47_03200 0.58 -0.8 664,197 - IAI47_03200 0.58 -0.4 664,197 - IAI47_03200 0.58 -1.2 664,197 - IAI47_03200 0.58 +0.2 664,197 - IAI47_03200 0.58 +0.8 664,197 - IAI47_03200 0.58 -1.8 664,197 - IAI47_03200 0.58 -0.2 664,197 - IAI47_03200 0.58 -0.5 664,232 + IAI47_03200 0.62 +0.3 664,232 + IAI47_03200 0.62 -0.9 664,232 + IAI47_03200 0.62 -0.8 664,233 - IAI47_03200 0.62 -0.4 664,391 + IAI47_03200 0.82 -0.2 664,392 - IAI47_03200 0.82 -1.2 664,392 - IAI47_03200 0.82 -1.6 664,404 + IAI47_03200 0.83 +1.2 664,419 - IAI47_03200 0.85 -0.5 664,477 + -0.2 664,478 - -3.3 664,478 - +1.2 664,503 + +1.0 664,503 + +0.7 664,503 + -0.6 664,503 + -0.7 664,503 + +0.5 664,504 - -0.5 664,504 - +1.0 664,504 - +0.3 664,504 - +0.5 664,504 - +0.5 664,542 + -0.2 664,542 + +0.0 664,542 + +1.0 664,542 + +1.0 664,542 + +0.7 664,542 + +0.2 664,542 + +2.2 664,542 + -0.2 664,542 + -0.0 664,542 + +1.1 664,543 - -0.8 664,543 - -1.5 664,543 - -0.7 664,543 - -0.8 664,589 + +0.8 664,589 + -0.2 664,589 + -2.3 664,589 + +0.0 664,589 + -0.3 664,589 + +0.4 664,590 - -0.5 664,590 - +0.6 664,590 - -0.8 664,590 - -0.6 664,596 - -2.1 664,596 - +0.0 664,618 + -0.3 664,618 + -0.6 664,618 + +2.8 664,618 + +0.7 664,619 - -0.5 664,619 - +0.8 664,619 - -1.0 664,619 - -0.3 664,619 - +0.2 664,619 - -0.2 664,630 + +0.9 664,648 + +0.5 664,649 - +1.1 664,649 - +1.0 664,649 - -0.9 664,649 - +1.4 664,649 - +0.4 664,663 - +0.1 664,668 + -0.2 664,669 - -1.8 664,746 + +1.4 664,746 + +0.9 664,797 - -0.7 664,797 - -0.4 664,850 - IAI47_03205 0.11 -0.5 664,885 + IAI47_03205 0.15 +0.7 664,885 + IAI47_03205 0.15 -0.3 664,885 + IAI47_03205 0.15 -0.5 664,886 - IAI47_03205 0.15 -1.1 664,886 - IAI47_03205 0.15 +1.9 664,886 - IAI47_03205 0.15 -0.1 664,886 - IAI47_03205 0.15 -1.4 664,886 - IAI47_03205 0.15 -0.5 664,886 - IAI47_03205 0.15 +0.9 664,886 - IAI47_03205 0.15 +0.1 664,886 - IAI47_03205 0.15 -1.3 664,931 - IAI47_03205 0.20 +1.0 664,931 - IAI47_03205 0.20 +0.4 664,954 + IAI47_03205 0.23 +0.5 664,954 + IAI47_03205 0.23 -0.5 664,955 - IAI47_03205 0.23 -1.0 664,955 - IAI47_03205 0.23 +0.1 664,969 + IAI47_03205 0.24 +0.3 664,969 + IAI47_03205 0.24 +1.3 664,969 + IAI47_03205 0.24 -0.9 664,970 - IAI47_03205 0.25 -1.5 665,027 + IAI47_03205 0.31 +0.8 665,028 - IAI47_03205 0.31 -0.7 665,247 + IAI47_03205 0.55 -0.3 665,247 + IAI47_03205 0.55 +0.3 665,248 - IAI47_03205 0.55 +2.4 665,248 - IAI47_03205 0.55 +0.7 665,248 - IAI47_03205 0.55 -0.1 665,251 + IAI47_03205 0.55 -0.2 665,251 + IAI47_03205 0.55 -0.9 665,251 + IAI47_03205 0.55 +2.0 665,251 + IAI47_03205 0.55 -0.9 665,252 - IAI47_03205 0.56 +0.9 665,252 - IAI47_03205 0.56 +0.8 665,252 - IAI47_03205 0.56 +0.3 665,252 - IAI47_03205 0.56 +1.4 665,252 - IAI47_03205 0.56 +1.1 665,252 - IAI47_03205 0.56 -1.4 665,252 - IAI47_03205 0.56 -1.2 665,252 - IAI47_03205 0.56 +0.5 665,252 - IAI47_03205 0.56 -0.2 665,252 - IAI47_03205 0.56 -1.0 665,254 + IAI47_03205 0.56 +0.1 665,254 + IAI47_03205 0.56 -0.1 665,254 + IAI47_03205 0.56 -0.1 665,254 + IAI47_03205 0.56 -0.1 665,254 + IAI47_03205 0.56 -0.5 665,254 + IAI47_03205 0.56 +0.7 665,254 + IAI47_03205 0.56 +1.5 665,254 + IAI47_03205 0.56 -0.0 665,255 - IAI47_03205 0.56 +0.0 665,255 - IAI47_03205 0.56 +0.4 665,255 - IAI47_03205 0.56 -1.5 665,255 - IAI47_03205 0.56 -0.2 665,255 - IAI47_03205 0.56 -1.0 665,255 - IAI47_03205 0.56 +0.7 665,255 - IAI47_03205 0.56 -0.3 665,255 - IAI47_03205 0.56 +0.4 665,255 - IAI47_03205 0.56 -0.5 665,255 - IAI47_03205 0.56 -0.7 665,255 - IAI47_03205 0.56 +0.4 665,255 - IAI47_03205 0.56 +0.7 665,255 - IAI47_03205 0.56 -1.5 665,255 - IAI47_03205 0.56 -0.5 665,356 + IAI47_03205 0.67 +1.0 665,357 - IAI47_03205 0.67 +2.9 665,357 - IAI47_03205 0.67 -0.5 665,424 + IAI47_03205 0.74 +0.0 665,425 - IAI47_03205 0.75 +1.3 665,456 + IAI47_03205 0.78 -0.6 665,456 + IAI47_03205 0.78 -0.7 665,456 + IAI47_03205 0.78 +0.2 665,456 + IAI47_03205 0.78 +1.9 665,456 + IAI47_03205 0.78 +0.8 665,456 + IAI47_03205 0.78 +1.7 665,456 + IAI47_03205 0.78 +1.9 665,457 - IAI47_03205 0.78 -0.2 665,457 - IAI47_03205 0.78 +0.3 665,457 - IAI47_03205 0.78 -1.3 665,457 - IAI47_03205 0.78 -1.8 665,461 + IAI47_03205 0.79 -0.4 665,461 + IAI47_03205 0.79 -1.4 665,461 + IAI47_03205 0.79 -1.5 665,461 + IAI47_03205 0.79 +0.6 665,461 + IAI47_03205 0.79 -0.1 665,461 + IAI47_03205 0.79 -0.0 665,462 - IAI47_03205 0.79 +0.4 665,462 - IAI47_03205 0.79 -0.0 665,462 - IAI47_03205 0.79 +1.3 665,462 - IAI47_03205 0.79 -0.5 665,462 - IAI47_03205 0.79 +0.6 665,462 - IAI47_03205 0.79 -0.2
Or see this region's nucleotide sequence