Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19810 and IAI47_19815 are separated by 163 nucleotides IAI47_19815 and IAI47_19820 are separated by 685 nucleotides
IAI47_19810: IAI47_19810 - substrate-binding domain-containing protein, at 188,031 to 188,954
_19810
IAI47_19815: IAI47_19815 - hypothetical protein, at 189,118 to 189,465
_19815
IAI47_19820: IAI47_19820 - MCP four helix bundle domain-containing protein, at 190,151 to 191,770
_19820
Position (kb) 189
190 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 188.124 kb on - strand, within IAI47_19810 at 188.135 kb on + strand, within IAI47_19810 at 188.135 kb on + strand, within IAI47_19810 at 188.135 kb on + strand, within IAI47_19810 at 188.136 kb on - strand, within IAI47_19810 at 188.136 kb on - strand, within IAI47_19810 at 188.136 kb on - strand, within IAI47_19810 at 188.255 kb on + strand, within IAI47_19810 at 188.255 kb on + strand, within IAI47_19810 at 188.256 kb on - strand, within IAI47_19810 at 188.280 kb on - strand, within IAI47_19810 at 188.280 kb on - strand, within IAI47_19810 at 188.297 kb on + strand, within IAI47_19810 at 188.298 kb on - strand, within IAI47_19810 at 188.298 kb on - strand, within IAI47_19810 at 188.298 kb on - strand, within IAI47_19810 at 188.311 kb on + strand, within IAI47_19810 at 188.311 kb on + strand, within IAI47_19810 at 188.311 kb on + strand, within IAI47_19810 at 188.312 kb on - strand, within IAI47_19810 at 188.393 kb on + strand, within IAI47_19810 at 188.393 kb on + strand, within IAI47_19810 at 188.394 kb on - strand, within IAI47_19810 at 188.394 kb on - strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.400 kb on + strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.401 kb on - strand, within IAI47_19810 at 188.466 kb on + strand, within IAI47_19810 at 188.466 kb on + strand, within IAI47_19810 at 188.466 kb on + strand, within IAI47_19810 at 188.466 kb on + strand, within IAI47_19810 at 188.467 kb on - strand, within IAI47_19810 at 188.467 kb on - strand, within IAI47_19810 at 188.474 kb on + strand, within IAI47_19810 at 188.474 kb on + strand, within IAI47_19810 at 188.474 kb on + strand, within IAI47_19810 at 188.474 kb on + strand, within IAI47_19810 at 188.475 kb on - strand, within IAI47_19810 at 188.479 kb on + strand, within IAI47_19810 at 188.479 kb on + strand, within IAI47_19810 at 188.479 kb on + strand, within IAI47_19810 at 188.479 kb on + strand, within IAI47_19810 at 188.479 kb on + strand, within IAI47_19810 at 188.479 kb on + strand, within IAI47_19810 at 188.479 kb on + strand, within IAI47_19810 at 188.480 kb on - strand, within IAI47_19810 at 188.480 kb on - strand, within IAI47_19810 at 188.480 kb on - strand, within IAI47_19810 at 188.485 kb on + strand, within IAI47_19810 at 188.485 kb on + strand, within IAI47_19810 at 188.526 kb on - strand, within IAI47_19810 at 188.552 kb on + strand, within IAI47_19810 at 188.552 kb on + strand, within IAI47_19810 at 188.553 kb on - strand, within IAI47_19810 at 188.556 kb on + strand, within IAI47_19810 at 188.557 kb on - strand, within IAI47_19810 at 188.557 kb on - strand, within IAI47_19810 at 188.557 kb on - strand, within IAI47_19810 at 188.557 kb on - strand, within IAI47_19810 at 188.558 kb on + strand, within IAI47_19810 at 188.559 kb on - strand, within IAI47_19810 at 188.559 kb on - strand, within IAI47_19810 at 188.573 kb on + strand, within IAI47_19810 at 188.639 kb on - strand, within IAI47_19810 at 188.639 kb on - strand, within IAI47_19810 at 188.639 kb on - strand, within IAI47_19810 at 188.699 kb on + strand, within IAI47_19810 at 188.700 kb on - strand, within IAI47_19810 at 188.731 kb on + strand, within IAI47_19810 at 188.777 kb on + strand, within IAI47_19810 at 188.778 kb on - strand, within IAI47_19810 at 188.802 kb on - strand, within IAI47_19810 at 188.886 kb on - strand at 188.898 kb on + strand at 188.898 kb on + strand at 188.898 kb on + strand at 188.899 kb on - strand at 188.899 kb on - strand at 188.909 kb on + strand at 188.909 kb on + strand at 188.952 kb on + strand at 188.952 kb on + strand at 188.953 kb on - strand at 188.974 kb on + strand at 188.974 kb on + strand at 188.975 kb on - strand at 189.030 kb on + strand at 189.030 kb on + strand at 189.030 kb on + strand at 189.030 kb on + strand at 189.030 kb on + strand at 189.031 kb on - strand at 189.031 kb on - strand at 189.040 kb on + strand at 189.040 kb on + strand at 189.041 kb on - strand at 189.041 kb on - strand at 189.041 kb on - strand at 189.119 kb on + strand at 189.135 kb on + strand at 189.135 kb on + strand at 189.135 kb on + strand at 189.136 kb on - strand at 189.136 kb on - strand at 189.162 kb on + strand, within IAI47_19815 at 189.162 kb on + strand, within IAI47_19815 at 189.163 kb on - strand, within IAI47_19815 at 189.163 kb on - strand, within IAI47_19815 at 189.164 kb on + strand, within IAI47_19815 at 189.164 kb on + strand, within IAI47_19815 at 189.165 kb on - strand, within IAI47_19815 at 189.165 kb on - strand, within IAI47_19815 at 189.174 kb on + strand, within IAI47_19815 at 189.235 kb on - strand, within IAI47_19815 at 189.289 kb on + strand, within IAI47_19815 at 189.289 kb on + strand, within IAI47_19815 at 189.290 kb on - strand, within IAI47_19815 at 189.290 kb on - strand, within IAI47_19815 at 189.319 kb on + strand, within IAI47_19815 at 189.319 kb on + strand, within IAI47_19815 at 189.319 kb on + strand, within IAI47_19815 at 189.319 kb on + strand, within IAI47_19815 at 189.320 kb on - strand, within IAI47_19815 at 189.320 kb on - strand, within IAI47_19815 at 189.320 kb on - strand, within IAI47_19815 at 189.320 kb on - strand, within IAI47_19815 at 189.320 kb on - strand, within IAI47_19815 at 189.320 kb on - strand, within IAI47_19815 at 189.324 kb on + strand, within IAI47_19815 at 189.325 kb on - strand, within IAI47_19815 at 189.361 kb on - strand, within IAI47_19815 at 189.397 kb on - strand, within IAI47_19815 at 189.416 kb on + strand, within IAI47_19815 at 189.416 kb on + strand, within IAI47_19815 at 189.417 kb on - strand, within IAI47_19815 at 189.417 kb on - strand, within IAI47_19815 at 189.443 kb on + strand at 189.443 kb on + strand at 189.443 kb on + strand at 189.443 kb on + strand at 189.444 kb on - strand at 189.444 kb on - strand at 189.459 kb on + strand at 189.459 kb on + strand at 189.460 kb on - strand at 189.460 kb on - strand at 189.465 kb on + strand at 189.465 kb on + strand at 189.465 kb on + strand at 189.465 kb on + strand at 189.465 kb on + strand at 189.466 kb on - strand at 189.466 kb on - strand at 189.466 kb on - strand at 189.490 kb on + strand at 189.490 kb on + strand at 189.491 kb on - strand at 189.493 kb on + strand at 189.493 kb on + strand at 189.493 kb on + strand at 189.494 kb on - strand at 189.553 kb on + strand at 189.589 kb on + strand at 189.593 kb on + strand at 189.594 kb on - strand at 189.596 kb on + strand at 189.596 kb on + strand at 189.596 kb on + strand at 189.597 kb on - strand at 189.614 kb on + strand at 189.615 kb on - strand at 189.615 kb on - strand at 189.617 kb on + strand at 189.629 kb on + strand at 189.664 kb on - strand at 189.664 kb on - strand at 189.689 kb on + strand at 189.689 kb on + strand at 189.689 kb on + strand at 189.690 kb on - strand at 189.710 kb on + strand at 189.711 kb on - strand at 189.714 kb on + strand at 189.715 kb on - strand at 189.715 kb on - strand at 189.715 kb on - strand at 189.715 kb on - strand at 189.715 kb on - strand at 189.722 kb on + strand at 189.722 kb on + strand at 189.722 kb on + strand at 189.722 kb on + strand at 189.722 kb on + strand at 189.722 kb on + strand at 189.722 kb on + strand at 189.722 kb on + strand at 189.723 kb on - strand at 189.723 kb on - strand at 189.723 kb on - strand at 189.727 kb on - strand at 189.757 kb on + strand at 189.776 kb on + strand at 189.776 kb on + strand at 189.777 kb on - strand at 189.777 kb on - strand at 189.782 kb on + strand at 189.789 kb on + strand at 189.800 kb on + strand at 189.800 kb on + strand at 189.801 kb on - strand at 189.801 kb on - strand at 189.830 kb on + strand at 189.830 kb on + strand at 189.830 kb on + strand at 189.839 kb on - strand at 189.886 kb on + strand at 189.887 kb on - strand at 189.933 kb on + strand at 189.933 kb on + strand at 189.933 kb on + strand at 189.933 kb on + strand at 189.933 kb on + strand at 189.933 kb on + strand at 189.934 kb on - strand at 189.934 kb on - strand at 189.934 kb on - strand at 189.964 kb on + strand at 189.965 kb on - strand at 189.980 kb on + strand at 189.981 kb on - strand at 189.998 kb on + strand at 189.998 kb on + strand at 189.998 kb on + strand at 189.999 kb on - strand at 189.999 kb on - strand at 189.999 kb on - strand at 190.048 kb on + strand at 190.099 kb on - strand at 190.123 kb on + strand at 190.140 kb on - strand at 190.165 kb on - strand at 190.165 kb on - strand at 190.165 kb on - strand at 190.165 kb on - strand at 190.169 kb on + strand at 190.169 kb on + strand at 190.170 kb on - strand at 190.299 kb on - strand at 190.299 kb on - strand at 190.340 kb on - strand, within IAI47_19820 at 190.348 kb on + strand, within IAI47_19820 at 190.348 kb on + strand, within IAI47_19820 at 190.348 kb on + strand, within IAI47_19820 at 190.351 kb on + strand, within IAI47_19820 at 190.351 kb on + strand, within IAI47_19820 at 190.352 kb on - strand, within IAI47_19820 at 190.352 kb on - strand, within IAI47_19820 at 190.364 kb on - strand, within IAI47_19820
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 188,124 - IAI47_19810 0.10 +0.6 188,135 + IAI47_19810 0.11 -0.2 188,135 + IAI47_19810 0.11 -0.1 188,135 + IAI47_19810 0.11 +3.3 188,136 - IAI47_19810 0.11 +1.2 188,136 - IAI47_19810 0.11 +0.1 188,136 - IAI47_19810 0.11 +1.1 188,255 + IAI47_19810 0.24 +0.1 188,255 + IAI47_19810 0.24 -1.5 188,256 - IAI47_19810 0.24 +0.5 188,280 - IAI47_19810 0.27 -0.9 188,280 - IAI47_19810 0.27 -0.7 188,297 + IAI47_19810 0.29 -0.1 188,298 - IAI47_19810 0.29 +0.8 188,298 - IAI47_19810 0.29 +0.2 188,298 - IAI47_19810 0.29 -1.3 188,311 + IAI47_19810 0.30 +0.1 188,311 + IAI47_19810 0.30 +0.1 188,311 + IAI47_19810 0.30 -1.1 188,312 - IAI47_19810 0.30 -1.3 188,393 + IAI47_19810 0.39 +1.1 188,393 + IAI47_19810 0.39 +2.1 188,394 - IAI47_19810 0.39 +0.8 188,394 - IAI47_19810 0.39 +0.6 188,400 + IAI47_19810 0.40 +0.5 188,400 + IAI47_19810 0.40 +1.0 188,400 + IAI47_19810 0.40 -0.3 188,400 + IAI47_19810 0.40 +1.1 188,400 + IAI47_19810 0.40 -0.1 188,400 + IAI47_19810 0.40 +1.4 188,400 + IAI47_19810 0.40 -1.9 188,400 + IAI47_19810 0.40 -1.8 188,400 + IAI47_19810 0.40 -0.4 188,400 + IAI47_19810 0.40 +0.2 188,400 + IAI47_19810 0.40 +0.7 188,400 + IAI47_19810 0.40 +0.8 188,400 + IAI47_19810 0.40 -1.6 188,400 + IAI47_19810 0.40 -2.0 188,400 + IAI47_19810 0.40 +1.1 188,400 + IAI47_19810 0.40 -0.1 188,400 + IAI47_19810 0.40 -0.5 188,400 + IAI47_19810 0.40 -1.7 188,401 - IAI47_19810 0.40 -0.5 188,401 - IAI47_19810 0.40 +1.4 188,401 - IAI47_19810 0.40 +1.9 188,401 - IAI47_19810 0.40 -1.4 188,401 - IAI47_19810 0.40 +0.1 188,401 - IAI47_19810 0.40 -0.6 188,401 - IAI47_19810 0.40 +1.6 188,401 - IAI47_19810 0.40 -1.6 188,401 - IAI47_19810 0.40 +0.4 188,401 - IAI47_19810 0.40 +0.0 188,401 - IAI47_19810 0.40 +0.5 188,401 - IAI47_19810 0.40 +0.3 188,401 - IAI47_19810 0.40 +0.9 188,401 - IAI47_19810 0.40 +0.4 188,401 - IAI47_19810 0.40 +0.8 188,401 - IAI47_19810 0.40 -1.3 188,401 - IAI47_19810 0.40 -1.3 188,401 - IAI47_19810 0.40 -0.7 188,401 - IAI47_19810 0.40 +0.2 188,466 + IAI47_19810 0.47 +0.8 188,466 + IAI47_19810 0.47 -0.3 188,466 + IAI47_19810 0.47 +1.3 188,466 + IAI47_19810 0.47 -0.5 188,467 - IAI47_19810 0.47 +0.4 188,467 - IAI47_19810 0.47 +0.8 188,474 + IAI47_19810 0.48 +0.7 188,474 + IAI47_19810 0.48 -0.2 188,474 + IAI47_19810 0.48 -1.8 188,474 + IAI47_19810 0.48 +0.7 188,475 - IAI47_19810 0.48 -0.1 188,479 + IAI47_19810 0.48 +1.1 188,479 + IAI47_19810 0.48 +0.9 188,479 + IAI47_19810 0.48 -0.1 188,479 + IAI47_19810 0.48 -1.4 188,479 + IAI47_19810 0.48 -0.2 188,479 + IAI47_19810 0.48 -0.8 188,479 + IAI47_19810 0.48 +0.6 188,480 - IAI47_19810 0.49 +0.1 188,480 - IAI47_19810 0.49 -1.8 188,480 - IAI47_19810 0.49 +1.7 188,485 + IAI47_19810 0.49 +1.3 188,485 + IAI47_19810 0.49 +1.1 188,526 - IAI47_19810 0.54 +1.3 188,552 + IAI47_19810 0.56 +0.2 188,552 + IAI47_19810 0.56 +0.2 188,553 - IAI47_19810 0.56 +1.3 188,556 + IAI47_19810 0.57 -1.7 188,557 - IAI47_19810 0.57 -0.6 188,557 - IAI47_19810 0.57 -1.7 188,557 - IAI47_19810 0.57 +0.5 188,557 - IAI47_19810 0.57 -0.3 188,558 + IAI47_19810 0.57 +3.0 188,559 - IAI47_19810 0.57 +2.4 188,559 - IAI47_19810 0.57 +0.1 188,573 + IAI47_19810 0.59 -1.9 188,639 - IAI47_19810 0.66 +0.4 188,639 - IAI47_19810 0.66 +0.7 188,639 - IAI47_19810 0.66 +0.2 188,699 + IAI47_19810 0.72 -0.5 188,700 - IAI47_19810 0.72 -1.1 188,731 + IAI47_19810 0.76 +1.5 188,777 + IAI47_19810 0.81 +0.4 188,778 - IAI47_19810 0.81 -1.1 188,802 - IAI47_19810 0.83 +1.2 188,886 - +1.7 188,898 + -1.7 188,898 + -1.2 188,898 + -0.2 188,899 - +0.4 188,899 - -1.6 188,909 + -0.8 188,909 + +0.1 188,952 + +1.4 188,952 + +0.7 188,953 - +0.5 188,974 + +1.0 188,974 + +2.4 188,975 - -1.6 189,030 + +2.4 189,030 + -2.2 189,030 + -1.6 189,030 + -1.7 189,030 + +0.3 189,031 - -2.2 189,031 - +0.6 189,040 + +1.9 189,040 + +0.2 189,041 - +1.8 189,041 - +1.5 189,041 - +0.8 189,119 + -0.0 189,135 + -0.2 189,135 + +1.3 189,135 + +1.7 189,136 - -2.8 189,136 - +0.7 189,162 + IAI47_19815 0.13 -1.4 189,162 + IAI47_19815 0.13 -0.6 189,163 - IAI47_19815 0.13 -0.5 189,163 - IAI47_19815 0.13 +2.7 189,164 + IAI47_19815 0.13 +1.3 189,164 + IAI47_19815 0.13 +2.4 189,165 - IAI47_19815 0.14 -0.3 189,165 - IAI47_19815 0.14 -0.6 189,174 + IAI47_19815 0.16 -0.9 189,235 - IAI47_19815 0.34 -0.8 189,289 + IAI47_19815 0.49 +0.1 189,289 + IAI47_19815 0.49 -0.8 189,290 - IAI47_19815 0.49 +1.4 189,290 - IAI47_19815 0.49 -0.8 189,319 + IAI47_19815 0.58 -0.1 189,319 + IAI47_19815 0.58 -1.0 189,319 + IAI47_19815 0.58 +0.2 189,319 + IAI47_19815 0.58 +1.4 189,320 - IAI47_19815 0.58 +0.2 189,320 - IAI47_19815 0.58 -0.2 189,320 - IAI47_19815 0.58 +1.3 189,320 - IAI47_19815 0.58 -1.9 189,320 - IAI47_19815 0.58 +1.6 189,320 - IAI47_19815 0.58 +4.7 189,324 + IAI47_19815 0.59 +1.8 189,325 - IAI47_19815 0.59 -0.9 189,361 - IAI47_19815 0.70 -1.9 189,397 - IAI47_19815 0.80 +2.3 189,416 + IAI47_19815 0.86 +0.9 189,416 + IAI47_19815 0.86 +1.6 189,417 - IAI47_19815 0.86 +0.5 189,417 - IAI47_19815 0.86 +1.5 189,443 + +0.1 189,443 + +1.9 189,443 + -1.8 189,443 + +0.8 189,444 - +1.1 189,444 - +0.9 189,459 + +2.2 189,459 + +1.6 189,460 - -0.4 189,460 - -0.4 189,465 + +0.9 189,465 + +0.8 189,465 + +1.0 189,465 + +1.4 189,465 + +2.1 189,466 - +0.1 189,466 - +2.4 189,466 - +0.4 189,490 + -0.8 189,490 + +2.1 189,491 - -0.6 189,493 + +1.9 189,493 + -1.3 189,493 + -0.1 189,494 - -1.3 189,553 + +0.9 189,589 + -0.4 189,593 + -1.0 189,594 - +1.1 189,596 + +0.5 189,596 + +1.1 189,596 + +0.4 189,597 - -0.8 189,614 + +2.2 189,615 - -0.5 189,615 - +1.3 189,617 + -1.6 189,629 + -1.9 189,664 - -1.3 189,664 - +0.8 189,689 + +1.2 189,689 + -1.5 189,689 + +0.2 189,690 - -1.9 189,710 + +0.3 189,711 - -0.1 189,714 + +0.6 189,715 - -0.9 189,715 - +2.2 189,715 - -1.8 189,715 - +0.2 189,715 - +0.4 189,722 + -0.1 189,722 + +0.6 189,722 + +1.8 189,722 + -1.2 189,722 + +1.8 189,722 + +0.4 189,722 + +0.5 189,722 + +0.1 189,723 - +0.9 189,723 - +0.7 189,723 - +1.2 189,727 - -1.8 189,757 + +0.0 189,776 + -1.9 189,776 + +0.6 189,777 - -0.6 189,777 - -0.1 189,782 + -2.4 189,789 + -0.5 189,800 + -0.6 189,800 + +0.9 189,801 - -0.3 189,801 - +0.1 189,830 + -0.1 189,830 + -2.4 189,830 + -0.3 189,839 - +0.2 189,886 + +2.1 189,887 - -1.4 189,933 + +0.5 189,933 + -0.7 189,933 + +0.7 189,933 + -0.8 189,933 + -0.8 189,933 + -0.8 189,934 - -2.4 189,934 - -0.2 189,934 - +1.7 189,964 + -2.8 189,965 - -0.2 189,980 + -0.6 189,981 - +0.7 189,998 + +1.8 189,998 + -1.2 189,998 + +0.2 189,999 - +1.1 189,999 - +1.6 189,999 - +0.5 190,048 + -2.1 190,099 - +1.0 190,123 + +0.3 190,140 - +0.3 190,165 - -1.8 190,165 - -0.4 190,165 - +0.3 190,165 - +0.9 190,169 + +1.7 190,169 + -0.4 190,170 - -0.0 190,299 - +1.0 190,299 - +1.8 190,340 - IAI47_19820 0.12 +0.2 190,348 + IAI47_19820 0.12 -0.5 190,348 + IAI47_19820 0.12 -1.9 190,348 + IAI47_19820 0.12 +1.2 190,351 + IAI47_19820 0.12 +0.1 190,351 + IAI47_19820 0.12 +1.0 190,352 - IAI47_19820 0.12 -0.4 190,352 - IAI47_19820 0.12 +0.4 190,364 - IAI47_19820 0.13 +1.9
Or see this region's nucleotide sequence