Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_21275 and IAI47_21280 are separated by 6 nucleotides IAI47_21280 and IAI47_21285 are separated by 21 nucleotides IAI47_21285 and IAI47_21290 are separated by 240 nucleotides IAI47_21290 and IAI47_21295 are separated by 149 nucleotides
IAI47_21275: IAI47_21275 - ABC transporter permease subunit, at 511,173 to 511,898
_21275
IAI47_21280: IAI47_21280 - amino acid ABC transporter substrate-binding protein, at 511,905 to 512,753
_21280
IAI47_21285: IAI47_21285 - amidohydrolase, at 512,775 to 513,962
_21285
IAI47_21290: IAI47_21290 - alpha/beta hydrolase, at 514,203 to 514,680
_21290
IAI47_21295: IAI47_21295 - DJ-1/PfpI family protein, at 514,830 to 515,549
_21295
Position (kb) 512
513
514 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 511.806 kb on + strand, within IAI47_21275 at 511.817 kb on + strand, within IAI47_21275 at 511.817 kb on + strand, within IAI47_21275 at 511.818 kb on - strand, within IAI47_21275 at 511.873 kb on - strand at 511.873 kb on - strand at 511.886 kb on + strand at 511.886 kb on + strand at 511.886 kb on + strand at 511.886 kb on + strand at 511.887 kb on - strand at 511.887 kb on - strand at 511.906 kb on + strand at 511.906 kb on + strand at 511.906 kb on + strand at 511.906 kb on + strand at 512.084 kb on + strand, within IAI47_21280 at 512.197 kb on + strand, within IAI47_21280 at 512.197 kb on + strand, within IAI47_21280 at 512.197 kb on + strand, within IAI47_21280 at 512.197 kb on + strand, within IAI47_21280 at 512.197 kb on + strand, within IAI47_21280 at 512.197 kb on + strand, within IAI47_21280 at 512.197 kb on + strand, within IAI47_21280 at 512.227 kb on + strand, within IAI47_21280 at 512.285 kb on + strand, within IAI47_21280 at 512.419 kb on + strand, within IAI47_21280 at 512.420 kb on - strand, within IAI47_21280 at 512.488 kb on + strand, within IAI47_21280 at 512.488 kb on + strand, within IAI47_21280 at 512.488 kb on + strand, within IAI47_21280 at 512.489 kb on - strand, within IAI47_21280 at 512.489 kb on - strand, within IAI47_21280 at 512.489 kb on - strand, within IAI47_21280 at 512.489 kb on - strand, within IAI47_21280 at 512.492 kb on + strand, within IAI47_21280 at 512.493 kb on - strand, within IAI47_21280 at 512.493 kb on - strand, within IAI47_21280 at 512.545 kb on + strand, within IAI47_21280 at 512.549 kb on + strand, within IAI47_21280 at 512.550 kb on - strand, within IAI47_21280 at 512.550 kb on - strand, within IAI47_21280 at 512.550 kb on - strand, within IAI47_21280 at 512.550 kb on - strand, within IAI47_21280 at 512.593 kb on + strand, within IAI47_21280 at 512.594 kb on - strand, within IAI47_21280 at 512.594 kb on - strand, within IAI47_21280 at 512.594 kb on - strand, within IAI47_21280 at 512.618 kb on + strand, within IAI47_21280 at 512.627 kb on + strand, within IAI47_21280 at 512.638 kb on + strand, within IAI47_21280 at 512.639 kb on - strand, within IAI47_21280 at 512.753 kb on + strand at 512.753 kb on + strand at 512.754 kb on - strand at 512.754 kb on - strand at 512.754 kb on - strand at 512.754 kb on - strand at 512.754 kb on - strand at 512.766 kb on + strand at 512.766 kb on + strand at 512.766 kb on + strand at 512.766 kb on + strand at 512.766 kb on + strand at 512.766 kb on + strand at 512.767 kb on - strand at 512.767 kb on - strand at 512.774 kb on - strand at 512.776 kb on + strand at 512.777 kb on - strand at 512.789 kb on - strand at 512.825 kb on + strand at 512.858 kb on + strand at 512.858 kb on + strand at 512.858 kb on + strand at 512.859 kb on - strand at 512.897 kb on + strand, within IAI47_21285 at 513.043 kb on - strand, within IAI47_21285 at 513.119 kb on + strand, within IAI47_21285 at 513.119 kb on + strand, within IAI47_21285 at 513.120 kb on - strand, within IAI47_21285 at 513.200 kb on + strand, within IAI47_21285 at 513.200 kb on + strand, within IAI47_21285 at 513.201 kb on - strand, within IAI47_21285 at 513.201 kb on - strand, within IAI47_21285 at 513.201 kb on - strand, within IAI47_21285 at 513.296 kb on + strand, within IAI47_21285 at 513.296 kb on + strand, within IAI47_21285 at 513.296 kb on + strand, within IAI47_21285 at 513.296 kb on + strand, within IAI47_21285 at 513.296 kb on + strand, within IAI47_21285 at 513.316 kb on - strand, within IAI47_21285 at 513.357 kb on + strand, within IAI47_21285 at 513.357 kb on + strand, within IAI47_21285 at 513.366 kb on + strand, within IAI47_21285 at 513.367 kb on - strand, within IAI47_21285 at 513.367 kb on - strand, within IAI47_21285 at 513.449 kb on + strand, within IAI47_21285 at 513.449 kb on + strand, within IAI47_21285 at 513.450 kb on - strand, within IAI47_21285 at 513.450 kb on - strand, within IAI47_21285 at 513.563 kb on + strand, within IAI47_21285 at 513.564 kb on - strand, within IAI47_21285 at 513.564 kb on - strand, within IAI47_21285 at 513.585 kb on + strand, within IAI47_21285 at 513.585 kb on + strand, within IAI47_21285 at 513.585 kb on + strand, within IAI47_21285 at 513.586 kb on - strand, within IAI47_21285 at 513.586 kb on - strand, within IAI47_21285 at 513.586 kb on - strand, within IAI47_21285 at 513.588 kb on + strand, within IAI47_21285 at 513.588 kb on + strand, within IAI47_21285 at 513.588 kb on + strand, within IAI47_21285 at 513.588 kb on + strand, within IAI47_21285 at 513.589 kb on - strand, within IAI47_21285 at 513.624 kb on - strand, within IAI47_21285 at 513.624 kb on - strand, within IAI47_21285 at 513.777 kb on + strand, within IAI47_21285 at 513.778 kb on - strand, within IAI47_21285 at 513.814 kb on + strand, within IAI47_21285 at 513.815 kb on - strand, within IAI47_21285 at 513.845 kb on + strand at 513.845 kb on + strand at 513.845 kb on + strand at 513.855 kb on + strand at 513.856 kb on - strand at 513.873 kb on + strand at 513.876 kb on - strand at 513.894 kb on + strand at 513.894 kb on + strand at 513.895 kb on - strand at 513.914 kb on + strand at 513.977 kb on + strand at 513.977 kb on + strand at 514.046 kb on + strand at 514.046 kb on + strand at 514.122 kb on + strand at 514.146 kb on + strand at 514.146 kb on + strand at 514.146 kb on + strand at 514.147 kb on - strand at 514.166 kb on + strand at 514.167 kb on - strand at 514.169 kb on + strand at 514.170 kb on - strand at 514.170 kb on - strand at 514.172 kb on + strand at 514.188 kb on - strand at 514.188 kb on - strand at 514.200 kb on + strand at 514.292 kb on + strand, within IAI47_21290 at 514.347 kb on + strand, within IAI47_21290 at 514.366 kb on - strand, within IAI47_21290 at 514.366 kb on - strand, within IAI47_21290 at 514.371 kb on + strand, within IAI47_21290 at 514.372 kb on - strand, within IAI47_21290 at 514.425 kb on + strand, within IAI47_21290 at 514.529 kb on + strand, within IAI47_21290 at 514.529 kb on + strand, within IAI47_21290 at 514.529 kb on + strand, within IAI47_21290 at 514.529 kb on + strand, within IAI47_21290 at 514.530 kb on - strand, within IAI47_21290 at 514.530 kb on - strand, within IAI47_21290 at 514.575 kb on + strand, within IAI47_21290 at 514.575 kb on + strand, within IAI47_21290 at 514.576 kb on - strand, within IAI47_21290 at 514.581 kb on + strand, within IAI47_21290 at 514.599 kb on - strand, within IAI47_21290 at 514.681 kb on + strand at 514.682 kb on - strand at 514.693 kb on + strand at 514.693 kb on + strand at 514.694 kb on - strand at 514.700 kb on - strand at 514.734 kb on - strand at 514.734 kb on - strand at 514.776 kb on - strand at 514.822 kb on + strand at 514.822 kb on + strand at 514.822 kb on + strand at 514.823 kb on - strand at 514.823 kb on - strand at 514.831 kb on + strand at 514.831 kb on + strand at 514.831 kb on + strand at 514.832 kb on - strand at 514.832 kb on - strand at 514.838 kb on + strand at 514.838 kb on + strand at 514.839 kb on - strand at 514.839 kb on - strand at 514.869 kb on + strand at 514.913 kb on + strand, within IAI47_21295 at 514.913 kb on + strand, within IAI47_21295 at 514.913 kb on + strand, within IAI47_21295 at 514.914 kb on - strand, within IAI47_21295
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 511,806 + IAI47_21275 0.87 -0.0 511,817 + IAI47_21275 0.89 +0.5 511,817 + IAI47_21275 0.89 -1.6 511,818 - IAI47_21275 0.89 +0.7 511,873 - +0.7 511,873 - -0.7 511,886 + +0.5 511,886 + -0.3 511,886 + -0.1 511,886 + -1.8 511,887 - -1.0 511,887 - +0.0 511,906 + +1.2 511,906 + +2.1 511,906 + -0.2 511,906 + -1.0 512,084 + IAI47_21280 0.21 +0.3 512,197 + IAI47_21280 0.34 +0.2 512,197 + IAI47_21280 0.34 -1.8 512,197 + IAI47_21280 0.34 -0.1 512,197 + IAI47_21280 0.34 +0.2 512,197 + IAI47_21280 0.34 -1.4 512,197 + IAI47_21280 0.34 +0.2 512,197 + IAI47_21280 0.34 +0.1 512,227 + IAI47_21280 0.38 +0.5 512,285 + IAI47_21280 0.45 +0.3 512,419 + IAI47_21280 0.61 +0.7 512,420 - IAI47_21280 0.61 +0.2 512,488 + IAI47_21280 0.69 -1.0 512,488 + IAI47_21280 0.69 -2.5 512,488 + IAI47_21280 0.69 +2.0 512,489 - IAI47_21280 0.69 +1.5 512,489 - IAI47_21280 0.69 +0.4 512,489 - IAI47_21280 0.69 +1.9 512,489 - IAI47_21280 0.69 -0.4 512,492 + IAI47_21280 0.69 -0.8 512,493 - IAI47_21280 0.69 -0.7 512,493 - IAI47_21280 0.69 +0.6 512,545 + IAI47_21280 0.75 +1.9 512,549 + IAI47_21280 0.76 +0.5 512,550 - IAI47_21280 0.76 -2.4 512,550 - IAI47_21280 0.76 +1.3 512,550 - IAI47_21280 0.76 -0.3 512,550 - IAI47_21280 0.76 -0.6 512,593 + IAI47_21280 0.81 -1.0 512,594 - IAI47_21280 0.81 +1.3 512,594 - IAI47_21280 0.81 +1.6 512,594 - IAI47_21280 0.81 +0.2 512,618 + IAI47_21280 0.84 -0.1 512,627 + IAI47_21280 0.85 -0.6 512,638 + IAI47_21280 0.86 -0.8 512,639 - IAI47_21280 0.86 -0.4 512,753 + -0.8 512,753 + -1.9 512,754 - -0.9 512,754 - -0.5 512,754 - +1.4 512,754 - +1.2 512,754 - -0.2 512,766 + +0.4 512,766 + -0.9 512,766 + +0.3 512,766 + -0.3 512,766 + +0.9 512,766 + -2.2 512,767 - -1.2 512,767 - +0.7 512,774 - -1.3 512,776 + +1.6 512,777 - +0.2 512,789 - -0.1 512,825 + +1.3 512,858 + +0.9 512,858 + -1.6 512,858 + -0.7 512,859 - -0.3 512,897 + IAI47_21285 0.10 -1.8 513,043 - IAI47_21285 0.23 -2.7 513,119 + IAI47_21285 0.29 -1.0 513,119 + IAI47_21285 0.29 +0.8 513,120 - IAI47_21285 0.29 -0.0 513,200 + IAI47_21285 0.36 -1.3 513,200 + IAI47_21285 0.36 +0.2 513,201 - IAI47_21285 0.36 +1.0 513,201 - IAI47_21285 0.36 +0.5 513,201 - IAI47_21285 0.36 +0.5 513,296 + IAI47_21285 0.44 +1.3 513,296 + IAI47_21285 0.44 +2.5 513,296 + IAI47_21285 0.44 +0.9 513,296 + IAI47_21285 0.44 +0.2 513,296 + IAI47_21285 0.44 +0.9 513,316 - IAI47_21285 0.46 -0.4 513,357 + IAI47_21285 0.49 -2.1 513,357 + IAI47_21285 0.49 -1.8 513,366 + IAI47_21285 0.50 -0.2 513,367 - IAI47_21285 0.50 +0.4 513,367 - IAI47_21285 0.50 +0.7 513,449 + IAI47_21285 0.57 +0.5 513,449 + IAI47_21285 0.57 +0.1 513,450 - IAI47_21285 0.57 +1.3 513,450 - IAI47_21285 0.57 -1.2 513,563 + IAI47_21285 0.66 -0.2 513,564 - IAI47_21285 0.66 +0.2 513,564 - IAI47_21285 0.66 -1.3 513,585 + IAI47_21285 0.68 +0.3 513,585 + IAI47_21285 0.68 -1.0 513,585 + IAI47_21285 0.68 +0.5 513,586 - IAI47_21285 0.68 +0.0 513,586 - IAI47_21285 0.68 +0.9 513,586 - IAI47_21285 0.68 -1.1 513,588 + IAI47_21285 0.68 +0.1 513,588 + IAI47_21285 0.68 -1.8 513,588 + IAI47_21285 0.68 +0.3 513,588 + IAI47_21285 0.68 -1.2 513,589 - IAI47_21285 0.69 +0.2 513,624 - IAI47_21285 0.71 +0.1 513,624 - IAI47_21285 0.71 +0.7 513,777 + IAI47_21285 0.84 +0.2 513,778 - IAI47_21285 0.84 +1.3 513,814 + IAI47_21285 0.87 +0.4 513,815 - IAI47_21285 0.88 +0.1 513,845 + +0.1 513,845 + +0.9 513,845 + -1.2 513,855 + +0.6 513,856 - -0.3 513,873 + +0.3 513,876 - +0.0 513,894 + +0.6 513,894 + -2.9 513,895 - -0.5 513,914 + -0.8 513,977 + +1.3 513,977 + +0.7 514,046 + -1.1 514,046 + -0.5 514,122 + +1.3 514,146 + +2.0 514,146 + -0.3 514,146 + -0.0 514,147 - -1.4 514,166 + -2.4 514,167 - -3.1 514,169 + -0.8 514,170 - +1.0 514,170 - -1.4 514,172 + +0.6 514,188 - -0.5 514,188 - -1.4 514,200 + +0.0 514,292 + IAI47_21290 0.19 -1.4 514,347 + IAI47_21290 0.30 +0.4 514,366 - IAI47_21290 0.34 -0.7 514,366 - IAI47_21290 0.34 +0.2 514,371 + IAI47_21290 0.35 +0.3 514,372 - IAI47_21290 0.35 -1.7 514,425 + IAI47_21290 0.46 -0.8 514,529 + IAI47_21290 0.68 -2.0 514,529 + IAI47_21290 0.68 -0.3 514,529 + IAI47_21290 0.68 +0.5 514,529 + IAI47_21290 0.68 -1.1 514,530 - IAI47_21290 0.68 +0.6 514,530 - IAI47_21290 0.68 -0.2 514,575 + IAI47_21290 0.78 +0.8 514,575 + IAI47_21290 0.78 -0.0 514,576 - IAI47_21290 0.78 -2.0 514,581 + IAI47_21290 0.79 +0.2 514,599 - IAI47_21290 0.83 -0.9 514,681 + +0.3 514,682 - -0.5 514,693 + +1.0 514,693 + -0.5 514,694 - -1.0 514,700 - -1.0 514,734 - -0.0 514,734 - -0.2 514,776 - +0.4 514,822 + -0.3 514,822 + +0.6 514,822 + -0.2 514,823 - +1.8 514,823 - -0.6 514,831 + +0.3 514,831 + -0.7 514,831 + -0.3 514,832 - -0.1 514,832 - +0.7 514,838 + -1.4 514,838 + +1.7 514,839 - +0.8 514,839 - +0.5 514,869 + -1.2 514,913 + IAI47_21295 0.12 +0.4 514,913 + IAI47_21295 0.12 -0.0 514,913 + IAI47_21295 0.12 -0.1 514,914 - IAI47_21295 0.12 -1.2
Or see this region's nucleotide sequence