Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_21045 and IAI47_21050 are separated by 234 nucleotides IAI47_21050 and IAI47_21055 are separated by 515 nucleotides
IAI47_21045: IAI47_21045 - nitrilase family protein, at 454,145 to 455,119
_21045
IAI47_21050: IAI47_21050 - beta-lactamase family protein, at 455,354 to 456,424
_21050
IAI47_21055: IAI47_21055 - MFS transporter, at 456,940 to 458,289
_21055
Position (kb) 455
456
457 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 454.367 kb on - strand, within IAI47_21045 at 454.367 kb on - strand, within IAI47_21045 at 454.414 kb on + strand, within IAI47_21045 at 454.414 kb on + strand, within IAI47_21045 at 454.415 kb on - strand, within IAI47_21045 at 454.429 kb on - strand, within IAI47_21045 at 454.457 kb on + strand, within IAI47_21045 at 454.457 kb on + strand, within IAI47_21045 at 454.458 kb on - strand, within IAI47_21045 at 454.458 kb on - strand, within IAI47_21045 at 454.459 kb on + strand, within IAI47_21045 at 454.459 kb on + strand, within IAI47_21045 at 454.460 kb on - strand, within IAI47_21045 at 454.460 kb on - strand, within IAI47_21045 at 454.460 kb on - strand, within IAI47_21045 at 454.460 kb on - strand, within IAI47_21045 at 454.460 kb on - strand, within IAI47_21045 at 454.466 kb on + strand, within IAI47_21045 at 454.466 kb on + strand, within IAI47_21045 at 454.466 kb on + strand, within IAI47_21045 at 454.466 kb on + strand, within IAI47_21045 at 454.466 kb on + strand, within IAI47_21045 at 454.466 kb on + strand, within IAI47_21045 at 454.466 kb on + strand, within IAI47_21045 at 454.466 kb on + strand, within IAI47_21045 at 454.466 kb on + strand, within IAI47_21045 at 454.467 kb on - strand, within IAI47_21045 at 454.467 kb on - strand, within IAI47_21045 at 454.467 kb on - strand, within IAI47_21045 at 454.467 kb on - strand, within IAI47_21045 at 454.467 kb on - strand, within IAI47_21045 at 454.467 kb on - strand, within IAI47_21045 at 454.477 kb on + strand, within IAI47_21045 at 454.508 kb on - strand, within IAI47_21045 at 454.567 kb on + strand, within IAI47_21045 at 454.581 kb on + strand, within IAI47_21045 at 454.581 kb on + strand, within IAI47_21045 at 454.581 kb on + strand, within IAI47_21045 at 454.581 kb on + strand, within IAI47_21045 at 454.581 kb on + strand, within IAI47_21045 at 454.581 kb on + strand, within IAI47_21045 at 454.582 kb on - strand, within IAI47_21045 at 454.582 kb on - strand, within IAI47_21045 at 454.582 kb on - strand, within IAI47_21045 at 454.612 kb on + strand, within IAI47_21045 at 454.612 kb on + strand, within IAI47_21045 at 454.612 kb on + strand, within IAI47_21045 at 454.613 kb on - strand, within IAI47_21045 at 454.613 kb on - strand, within IAI47_21045 at 454.613 kb on - strand, within IAI47_21045 at 454.613 kb on - strand, within IAI47_21045 at 454.637 kb on - strand, within IAI47_21045 at 454.637 kb on - strand, within IAI47_21045 at 454.657 kb on + strand, within IAI47_21045 at 454.657 kb on + strand, within IAI47_21045 at 454.657 kb on + strand, within IAI47_21045 at 454.658 kb on - strand, within IAI47_21045 at 454.658 kb on - strand, within IAI47_21045 at 454.658 kb on - strand, within IAI47_21045 at 454.658 kb on - strand, within IAI47_21045 at 454.658 kb on - strand, within IAI47_21045 at 454.729 kb on - strand, within IAI47_21045 at 454.768 kb on + strand, within IAI47_21045 at 454.768 kb on + strand, within IAI47_21045 at 454.768 kb on + strand, within IAI47_21045 at 454.845 kb on + strand, within IAI47_21045 at 454.845 kb on + strand, within IAI47_21045 at 454.846 kb on - strand, within IAI47_21045 at 454.853 kb on - strand, within IAI47_21045 at 454.858 kb on + strand, within IAI47_21045 at 454.859 kb on - strand, within IAI47_21045 at 454.861 kb on + strand, within IAI47_21045 at 454.861 kb on + strand, within IAI47_21045 at 454.922 kb on + strand, within IAI47_21045 at 454.922 kb on + strand, within IAI47_21045 at 454.962 kb on + strand, within IAI47_21045 at 454.963 kb on - strand, within IAI47_21045 at 454.963 kb on - strand, within IAI47_21045 at 454.963 kb on - strand, within IAI47_21045 at 454.963 kb on - strand, within IAI47_21045 at 454.986 kb on + strand, within IAI47_21045 at 454.986 kb on + strand, within IAI47_21045 at 455.044 kb on - strand at 455.044 kb on - strand at 455.044 kb on - strand at 455.045 kb on + strand at 455.052 kb on + strand at 455.052 kb on + strand at 455.052 kb on + strand at 455.053 kb on - strand at 455.053 kb on - strand at 455.053 kb on - strand at 455.053 kb on - strand at 455.053 kb on - strand at 455.053 kb on - strand at 455.072 kb on + strand at 455.072 kb on + strand at 455.073 kb on - strand at 455.073 kb on - strand at 455.112 kb on + strand at 455.112 kb on + strand at 455.113 kb on - strand at 455.113 kb on - strand at 455.113 kb on - strand at 455.117 kb on + strand at 455.117 kb on + strand at 455.118 kb on - strand at 455.118 kb on - strand at 455.224 kb on + strand at 455.225 kb on - strand at 455.259 kb on - strand at 455.277 kb on + strand at 455.277 kb on + strand at 455.278 kb on - strand at 455.278 kb on - strand at 455.278 kb on - strand at 455.329 kb on + strand at 455.329 kb on + strand at 455.330 kb on - strand at 455.330 kb on - strand at 455.330 kb on - strand at 455.330 kb on - strand at 455.356 kb on - strand at 455.356 kb on - strand at 455.397 kb on - strand at 455.406 kb on - strand at 455.406 kb on - strand at 455.425 kb on - strand at 455.444 kb on - strand at 455.444 kb on - strand at 455.455 kb on + strand at 455.456 kb on - strand at 455.471 kb on + strand, within IAI47_21050 at 455.472 kb on - strand, within IAI47_21050 at 455.472 kb on - strand, within IAI47_21050 at 455.472 kb on - strand, within IAI47_21050 at 455.472 kb on - strand, within IAI47_21050 at 455.472 kb on - strand, within IAI47_21050 at 455.477 kb on + strand, within IAI47_21050 at 455.514 kb on + strand, within IAI47_21050 at 455.515 kb on - strand, within IAI47_21050 at 455.515 kb on - strand, within IAI47_21050 at 455.520 kb on - strand, within IAI47_21050 at 455.574 kb on - strand, within IAI47_21050 at 455.587 kb on - strand, within IAI47_21050 at 455.596 kb on - strand, within IAI47_21050 at 455.609 kb on - strand, within IAI47_21050 at 455.614 kb on + strand, within IAI47_21050 at 455.624 kb on + strand, within IAI47_21050 at 455.624 kb on + strand, within IAI47_21050 at 455.625 kb on - strand, within IAI47_21050 at 455.657 kb on + strand, within IAI47_21050 at 455.660 kb on + strand, within IAI47_21050 at 455.668 kb on + strand, within IAI47_21050 at 455.669 kb on - strand, within IAI47_21050 at 455.770 kb on + strand, within IAI47_21050 at 455.779 kb on - strand, within IAI47_21050 at 455.812 kb on + strand, within IAI47_21050 at 455.812 kb on + strand, within IAI47_21050 at 455.813 kb on - strand, within IAI47_21050 at 455.854 kb on + strand, within IAI47_21050 at 455.855 kb on - strand, within IAI47_21050 at 455.863 kb on + strand, within IAI47_21050 at 455.864 kb on - strand, within IAI47_21050 at 455.885 kb on - strand, within IAI47_21050 at 455.998 kb on + strand, within IAI47_21050 at 456.083 kb on - strand, within IAI47_21050 at 456.104 kb on - strand, within IAI47_21050 at 456.176 kb on - strand, within IAI47_21050 at 456.209 kb on + strand, within IAI47_21050 at 456.209 kb on + strand, within IAI47_21050 at 456.217 kb on + strand, within IAI47_21050 at 456.283 kb on + strand, within IAI47_21050 at 456.284 kb on - strand, within IAI47_21050 at 456.293 kb on + strand, within IAI47_21050 at 456.319 kb on - strand at 456.390 kb on - strand at 456.411 kb on - strand at 456.429 kb on + strand at 456.440 kb on + strand at 456.495 kb on - strand at 456.496 kb on + strand at 456.501 kb on - strand at 456.501 kb on - strand at 456.522 kb on + strand at 456.527 kb on + strand at 456.528 kb on - strand at 456.530 kb on + strand at 456.547 kb on - strand at 456.552 kb on + strand at 456.554 kb on + strand at 456.555 kb on - strand at 456.580 kb on + strand at 456.599 kb on + strand at 456.599 kb on + strand at 456.654 kb on + strand at 456.658 kb on - strand at 456.680 kb on + strand at 456.681 kb on - strand at 456.681 kb on - strand at 456.706 kb on + strand at 456.807 kb on + strand at 456.807 kb on + strand at 456.807 kb on + strand at 456.807 kb on + strand at 456.808 kb on - strand at 456.808 kb on - strand at 456.813 kb on - strand at 456.854 kb on - strand at 456.859 kb on + strand at 456.859 kb on + strand at 456.859 kb on + strand at 456.859 kb on + strand at 456.866 kb on + strand at 456.866 kb on + strand at 456.866 kb on + strand at 456.873 kb on - strand at 456.900 kb on + strand at 456.901 kb on - strand at 456.915 kb on + strand at 456.915 kb on + strand at 456.916 kb on - strand at 456.916 kb on - strand at 456.928 kb on - strand at 456.939 kb on + strand at 456.939 kb on + strand at 456.939 kb on + strand at 456.939 kb on + strand at 456.940 kb on - strand at 456.940 kb on - strand at 456.940 kb on - strand at 456.940 kb on - strand at 457.022 kb on + strand at 457.022 kb on + strand at 457.023 kb on - strand at 457.023 kb on - strand at 457.023 kb on - strand at 457.025 kb on - strand at 457.025 kb on - strand at 457.075 kb on - strand, within IAI47_21055 at 457.075 kb on - strand, within IAI47_21055 at 457.114 kb on - strand, within IAI47_21055 at 457.122 kb on + strand, within IAI47_21055 at 457.122 kb on + strand, within IAI47_21055 at 457.122 kb on + strand, within IAI47_21055 at 457.123 kb on - strand, within IAI47_21055 at 457.123 kb on - strand, within IAI47_21055 at 457.139 kb on + strand, within IAI47_21055 at 457.152 kb on + strand, within IAI47_21055 at 457.153 kb on - strand, within IAI47_21055 at 457.191 kb on + strand, within IAI47_21055 at 457.192 kb on - strand, within IAI47_21055 at 457.192 kb on - strand, within IAI47_21055 at 457.208 kb on + strand, within IAI47_21055 at 457.208 kb on + strand, within IAI47_21055 at 457.208 kb on + strand, within IAI47_21055 at 457.208 kb on + strand, within IAI47_21055 at 457.209 kb on - strand, within IAI47_21055 at 457.209 kb on - strand, within IAI47_21055 at 457.209 kb on - strand, within IAI47_21055 at 457.209 kb on - strand, within IAI47_21055 at 457.248 kb on - strand, within IAI47_21055 at 457.248 kb on - strand, within IAI47_21055 at 457.248 kb on - strand, within IAI47_21055 at 457.260 kb on + strand, within IAI47_21055 at 457.260 kb on + strand, within IAI47_21055 at 457.270 kb on + strand, within IAI47_21055 at 457.271 kb on - strand, within IAI47_21055 at 457.302 kb on + strand, within IAI47_21055 at 457.329 kb on + strand, within IAI47_21055 at 457.329 kb on + strand, within IAI47_21055 at 457.330 kb on - strand, within IAI47_21055
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 454,367 - IAI47_21045 0.23 -1.2 454,367 - IAI47_21045 0.23 -0.5 454,414 + IAI47_21045 0.28 +0.2 454,414 + IAI47_21045 0.28 -1.0 454,415 - IAI47_21045 0.28 +0.2 454,429 - IAI47_21045 0.29 +0.4 454,457 + IAI47_21045 0.32 +0.0 454,457 + IAI47_21045 0.32 -0.3 454,458 - IAI47_21045 0.32 -1.3 454,458 - IAI47_21045 0.32 -0.2 454,459 + IAI47_21045 0.32 +0.1 454,459 + IAI47_21045 0.32 +0.3 454,460 - IAI47_21045 0.32 +1.2 454,460 - IAI47_21045 0.32 -1.5 454,460 - IAI47_21045 0.32 -0.8 454,460 - IAI47_21045 0.32 +0.2 454,460 - IAI47_21045 0.32 -0.3 454,466 + IAI47_21045 0.33 -0.8 454,466 + IAI47_21045 0.33 -0.2 454,466 + IAI47_21045 0.33 -2.5 454,466 + IAI47_21045 0.33 +0.1 454,466 + IAI47_21045 0.33 +0.2 454,466 + IAI47_21045 0.33 -1.5 454,466 + IAI47_21045 0.33 -0.2 454,466 + IAI47_21045 0.33 -0.5 454,466 + IAI47_21045 0.33 -1.8 454,467 - IAI47_21045 0.33 -1.6 454,467 - IAI47_21045 0.33 -0.7 454,467 - IAI47_21045 0.33 +0.5 454,467 - IAI47_21045 0.33 +0.2 454,467 - IAI47_21045 0.33 +0.2 454,467 - IAI47_21045 0.33 -0.7 454,477 + IAI47_21045 0.34 +1.8 454,508 - IAI47_21045 0.37 -0.5 454,567 + IAI47_21045 0.43 +1.5 454,581 + IAI47_21045 0.45 +0.8 454,581 + IAI47_21045 0.45 +0.3 454,581 + IAI47_21045 0.45 +3.0 454,581 + IAI47_21045 0.45 +0.9 454,581 + IAI47_21045 0.45 -0.3 454,581 + IAI47_21045 0.45 -0.3 454,582 - IAI47_21045 0.45 -1.9 454,582 - IAI47_21045 0.45 -2.0 454,582 - IAI47_21045 0.45 -1.0 454,612 + IAI47_21045 0.48 -0.7 454,612 + IAI47_21045 0.48 +0.2 454,612 + IAI47_21045 0.48 +0.8 454,613 - IAI47_21045 0.48 -0.3 454,613 - IAI47_21045 0.48 -0.4 454,613 - IAI47_21045 0.48 +0.5 454,613 - IAI47_21045 0.48 +2.5 454,637 - IAI47_21045 0.50 +1.3 454,637 - IAI47_21045 0.50 +1.5 454,657 + IAI47_21045 0.53 +0.5 454,657 + IAI47_21045 0.53 -1.2 454,657 + IAI47_21045 0.53 +0.0 454,658 - IAI47_21045 0.53 -2.4 454,658 - IAI47_21045 0.53 +0.7 454,658 - IAI47_21045 0.53 -2.8 454,658 - IAI47_21045 0.53 -0.3 454,658 - IAI47_21045 0.53 +0.1 454,729 - IAI47_21045 0.60 -1.3 454,768 + IAI47_21045 0.64 +0.9 454,768 + IAI47_21045 0.64 +1.2 454,768 + IAI47_21045 0.64 +0.4 454,845 + IAI47_21045 0.72 -1.1 454,845 + IAI47_21045 0.72 -1.3 454,846 - IAI47_21045 0.72 -0.4 454,853 - IAI47_21045 0.73 +0.5 454,858 + IAI47_21045 0.73 +2.2 454,859 - IAI47_21045 0.73 -1.1 454,861 + IAI47_21045 0.73 -0.5 454,861 + IAI47_21045 0.73 -1.1 454,922 + IAI47_21045 0.80 -0.9 454,922 + IAI47_21045 0.80 +0.2 454,962 + IAI47_21045 0.84 +0.8 454,963 - IAI47_21045 0.84 -2.1 454,963 - IAI47_21045 0.84 -1.2 454,963 - IAI47_21045 0.84 -1.5 454,963 - IAI47_21045 0.84 +1.2 454,986 + IAI47_21045 0.86 -1.8 454,986 + IAI47_21045 0.86 -0.5 455,044 - +0.9 455,044 - +0.8 455,044 - -1.5 455,045 + -0.2 455,052 + +0.1 455,052 + +0.6 455,052 + +0.3 455,053 - +0.2 455,053 - +0.4 455,053 - +2.0 455,053 - +0.2 455,053 - -1.3 455,053 - -1.0 455,072 + +0.6 455,072 + +0.5 455,073 - -2.3 455,073 - -0.4 455,112 + -1.0 455,112 + -0.6 455,113 - -1.3 455,113 - -1.3 455,113 - +2.7 455,117 + +0.8 455,117 + -0.5 455,118 - +0.2 455,118 - -0.1 455,224 + +2.5 455,225 - +0.9 455,259 - +0.0 455,277 + +1.0 455,277 + +2.5 455,278 - -1.2 455,278 - -1.1 455,278 - -0.5 455,329 + -3.6 455,329 + -2.0 455,330 - -1.3 455,330 - +0.4 455,330 - -1.1 455,330 - -1.0 455,356 - -0.3 455,356 - +0.1 455,397 - -0.8 455,406 - -0.8 455,406 - -0.1 455,425 - +1.3 455,444 - +0.8 455,444 - +1.0 455,455 + -2.9 455,456 - -1.5 455,471 + IAI47_21050 0.11 -0.0 455,472 - IAI47_21050 0.11 -0.8 455,472 - IAI47_21050 0.11 +0.3 455,472 - IAI47_21050 0.11 -0.4 455,472 - IAI47_21050 0.11 -2.3 455,472 - IAI47_21050 0.11 +0.7 455,477 + IAI47_21050 0.11 +1.5 455,514 + IAI47_21050 0.15 -1.7 455,515 - IAI47_21050 0.15 +0.1 455,515 - IAI47_21050 0.15 +1.2 455,520 - IAI47_21050 0.15 +1.0 455,574 - IAI47_21050 0.21 -1.6 455,587 - IAI47_21050 0.22 +1.3 455,596 - IAI47_21050 0.23 -1.0 455,609 - IAI47_21050 0.24 +1.7 455,614 + IAI47_21050 0.24 -0.4 455,624 + IAI47_21050 0.25 -1.5 455,624 + IAI47_21050 0.25 +2.5 455,625 - IAI47_21050 0.25 -1.9 455,657 + IAI47_21050 0.28 +1.4 455,660 + IAI47_21050 0.29 +0.3 455,668 + IAI47_21050 0.29 -0.2 455,669 - IAI47_21050 0.29 -1.0 455,770 + IAI47_21050 0.39 +0.4 455,779 - IAI47_21050 0.40 +0.4 455,812 + IAI47_21050 0.43 +0.1 455,812 + IAI47_21050 0.43 +1.3 455,813 - IAI47_21050 0.43 -1.7 455,854 + IAI47_21050 0.47 -1.0 455,855 - IAI47_21050 0.47 +0.2 455,863 + IAI47_21050 0.48 -1.6 455,864 - IAI47_21050 0.48 -0.3 455,885 - IAI47_21050 0.50 +0.2 455,998 + IAI47_21050 0.60 +0.1 456,083 - IAI47_21050 0.68 +1.9 456,104 - IAI47_21050 0.70 -0.0 456,176 - IAI47_21050 0.77 +0.0 456,209 + IAI47_21050 0.80 -0.3 456,209 + IAI47_21050 0.80 +1.2 456,217 + IAI47_21050 0.81 -2.3 456,283 + IAI47_21050 0.87 +0.7 456,284 - IAI47_21050 0.87 -0.2 456,293 + IAI47_21050 0.88 +0.3 456,319 - +0.1 456,390 - +1.3 456,411 - -0.5 456,429 + -0.4 456,440 + +0.3 456,495 - -0.0 456,496 + +0.4 456,501 - -0.0 456,501 - -1.5 456,522 + +1.3 456,527 + +1.0 456,528 - +1.0 456,530 + +0.3 456,547 - -1.2 456,552 + -1.8 456,554 + +0.1 456,555 - -1.2 456,580 + -1.7 456,599 + +0.5 456,599 + -0.7 456,654 + +1.7 456,658 - +1.0 456,680 + -1.8 456,681 - -0.0 456,681 - -0.3 456,706 + -2.6 456,807 + -0.4 456,807 + -0.2 456,807 + +0.2 456,807 + +0.1 456,808 - +1.3 456,808 - -1.5 456,813 - +2.9 456,854 - -1.7 456,859 + +1.1 456,859 + -0.2 456,859 + +0.2 456,859 + +0.5 456,866 + -0.5 456,866 + -0.7 456,866 + +0.9 456,873 - -0.8 456,900 + -1.4 456,901 - -0.4 456,915 + -1.2 456,915 + -1.0 456,916 - -2.1 456,916 - -1.3 456,928 - -0.7 456,939 + -0.3 456,939 + -1.2 456,939 + +0.1 456,939 + +0.2 456,940 - -1.1 456,940 - -0.2 456,940 - +0.1 456,940 - -0.8 457,022 + +0.1 457,022 + +2.2 457,023 - -0.3 457,023 - -1.0 457,023 - +0.2 457,025 - +1.0 457,025 - -1.2 457,075 - IAI47_21055 0.10 +0.0 457,075 - IAI47_21055 0.10 -0.5 457,114 - IAI47_21055 0.13 +0.1 457,122 + IAI47_21055 0.13 -0.6 457,122 + IAI47_21055 0.13 -0.2 457,122 + IAI47_21055 0.13 +0.2 457,123 - IAI47_21055 0.14 -0.8 457,123 - IAI47_21055 0.14 +2.2 457,139 + IAI47_21055 0.15 +2.5 457,152 + IAI47_21055 0.16 +0.5 457,153 - IAI47_21055 0.16 +0.2 457,191 + IAI47_21055 0.19 -2.6 457,192 - IAI47_21055 0.19 -1.4 457,192 - IAI47_21055 0.19 +0.8 457,208 + IAI47_21055 0.20 +0.0 457,208 + IAI47_21055 0.20 -1.9 457,208 + IAI47_21055 0.20 +0.9 457,208 + IAI47_21055 0.20 +0.5 457,209 - IAI47_21055 0.20 +0.1 457,209 - IAI47_21055 0.20 -1.4 457,209 - IAI47_21055 0.20 -0.5 457,209 - IAI47_21055 0.20 -0.6 457,248 - IAI47_21055 0.23 -0.2 457,248 - IAI47_21055 0.23 -2.1 457,248 - IAI47_21055 0.23 -0.9 457,260 + IAI47_21055 0.24 +2.7 457,260 + IAI47_21055 0.24 -2.4 457,270 + IAI47_21055 0.24 +1.0 457,271 - IAI47_21055 0.25 -0.9 457,302 + IAI47_21055 0.27 +0.4 457,329 + IAI47_21055 0.29 +0.2 457,329 + IAI47_21055 0.29 -0.3 457,330 - IAI47_21055 0.29 -2.0
Or see this region's nucleotide sequence