Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20075 and IAI47_20080 are separated by 239 nucleotides IAI47_20080 and IAI47_20085 are separated by 2 nucleotides IAI47_20085 and IAI47_20090 are separated by 2 nucleotides
IAI47_20075: IAI47_20075 - methyl-accepting chemotaxis protein, at 246,775 to 248,715
_20075
IAI47_20080: IAI47_20080 - sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC, at 248,955 to 250,037
_20080
IAI47_20085: IAI47_20085 - carbohydrate kinase family protein, at 250,040 to 250,987
_20085
IAI47_20090: IAI47_20090 - carbohydrate ABC transporter permease, at 250,990 to 251,829
_20090
Position (kb) 248
249
250
251 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 247.989 kb on - strand, within IAI47_20075 at 247.989 kb on - strand, within IAI47_20075 at 248.083 kb on - strand at 248.083 kb on - strand, within IAI47_20075 at 248.103 kb on + strand, within IAI47_20075 at 248.103 kb on + strand, within IAI47_20075 at 248.103 kb on + strand, within IAI47_20075 at 248.163 kb on + strand, within IAI47_20075 at 248.250 kb on + strand, within IAI47_20075 at 248.251 kb on - strand, within IAI47_20075 at 248.286 kb on + strand, within IAI47_20075 at 248.370 kb on + strand, within IAI47_20075 at 248.370 kb on + strand, within IAI47_20075 at 248.370 kb on + strand, within IAI47_20075 at 248.462 kb on + strand, within IAI47_20075 at 248.475 kb on + strand, within IAI47_20075 at 248.476 kb on - strand, within IAI47_20075 at 248.538 kb on + strand at 248.539 kb on - strand at 248.539 kb on - strand at 248.681 kb on + strand at 248.681 kb on + strand at 248.681 kb on + strand at 248.681 kb on + strand at 248.713 kb on + strand at 248.713 kb on + strand at 248.714 kb on - strand at 248.735 kb on + strand at 248.735 kb on + strand at 248.735 kb on + strand at 248.735 kb on + strand at 248.735 kb on + strand at 248.735 kb on + strand at 248.736 kb on - strand at 248.742 kb on + strand at 248.742 kb on + strand at 248.743 kb on - strand at 248.834 kb on + strand at 248.834 kb on + strand at 248.834 kb on + strand at 248.834 kb on + strand at 248.835 kb on - strand at 248.835 kb on - strand at 248.835 kb on - strand at 248.835 kb on - strand at 248.870 kb on + strand at 248.888 kb on + strand at 248.888 kb on + strand at 248.888 kb on + strand at 248.888 kb on + strand at 248.888 kb on + strand at 248.888 kb on + strand at 248.889 kb on - strand at 248.889 kb on - strand at 248.896 kb on + strand at 248.896 kb on + strand at 248.896 kb on + strand at 248.896 kb on + strand at 248.896 kb on + strand at 248.897 kb on - strand at 248.897 kb on - strand at 248.912 kb on + strand at 248.913 kb on - strand at 248.953 kb on - strand at 249.016 kb on - strand at 249.032 kb on + strand at 249.054 kb on - strand at 249.071 kb on + strand, within IAI47_20080 at 249.071 kb on + strand, within IAI47_20080 at 249.071 kb on + strand, within IAI47_20080 at 249.071 kb on + strand, within IAI47_20080 at 249.071 kb on + strand, within IAI47_20080 at 249.071 kb on + strand, within IAI47_20080 at 249.072 kb on - strand, within IAI47_20080 at 249.134 kb on + strand, within IAI47_20080 at 249.135 kb on - strand, within IAI47_20080 at 249.135 kb on - strand, within IAI47_20080 at 249.135 kb on - strand, within IAI47_20080 at 249.139 kb on - strand, within IAI47_20080 at 249.263 kb on + strand, within IAI47_20080 at 249.339 kb on - strand, within IAI47_20080 at 249.359 kb on + strand, within IAI47_20080 at 249.373 kb on + strand, within IAI47_20080 at 249.373 kb on + strand, within IAI47_20080 at 249.373 kb on + strand, within IAI47_20080 at 249.373 kb on + strand, within IAI47_20080 at 249.374 kb on - strand, within IAI47_20080 at 249.420 kb on + strand, within IAI47_20080 at 249.472 kb on + strand, within IAI47_20080 at 249.472 kb on + strand, within IAI47_20080 at 249.472 kb on + strand, within IAI47_20080 at 249.472 kb on + strand, within IAI47_20080 at 249.473 kb on - strand, within IAI47_20080 at 249.473 kb on - strand, within IAI47_20080 at 249.473 kb on - strand, within IAI47_20080 at 249.473 kb on - strand, within IAI47_20080 at 249.473 kb on - strand, within IAI47_20080 at 249.509 kb on + strand, within IAI47_20080 at 249.510 kb on - strand, within IAI47_20080 at 249.510 kb on - strand, within IAI47_20080 at 249.510 kb on - strand, within IAI47_20080 at 249.510 kb on - strand, within IAI47_20080 at 249.530 kb on + strand, within IAI47_20080 at 249.530 kb on + strand, within IAI47_20080 at 249.530 kb on + strand, within IAI47_20080 at 249.530 kb on + strand, within IAI47_20080 at 249.530 kb on + strand, within IAI47_20080 at 249.531 kb on - strand, within IAI47_20080 at 249.534 kb on + strand, within IAI47_20080 at 249.534 kb on + strand, within IAI47_20080 at 249.535 kb on - strand, within IAI47_20080 at 249.535 kb on - strand, within IAI47_20080 at 249.535 kb on - strand, within IAI47_20080 at 249.535 kb on - strand, within IAI47_20080 at 249.535 kb on - strand, within IAI47_20080 at 249.576 kb on + strand, within IAI47_20080 at 249.576 kb on + strand, within IAI47_20080 at 249.576 kb on + strand, within IAI47_20080 at 249.576 kb on + strand, within IAI47_20080 at 249.577 kb on - strand, within IAI47_20080 at 249.577 kb on - strand, within IAI47_20080 at 249.577 kb on - strand, within IAI47_20080 at 249.577 kb on - strand, within IAI47_20080 at 249.577 kb on - strand, within IAI47_20080 at 249.613 kb on - strand, within IAI47_20080 at 249.657 kb on + strand, within IAI47_20080 at 249.658 kb on - strand, within IAI47_20080 at 249.658 kb on - strand, within IAI47_20080 at 249.732 kb on + strand, within IAI47_20080 at 249.787 kb on + strand, within IAI47_20080 at 249.787 kb on + strand, within IAI47_20080 at 249.787 kb on + strand, within IAI47_20080 at 249.787 kb on + strand, within IAI47_20080 at 249.787 kb on + strand, within IAI47_20080 at 249.787 kb on + strand, within IAI47_20080 at 249.788 kb on - strand, within IAI47_20080 at 249.788 kb on - strand, within IAI47_20080 at 249.788 kb on - strand, within IAI47_20080 at 249.809 kb on + strand, within IAI47_20080 at 249.849 kb on - strand, within IAI47_20080 at 249.849 kb on - strand, within IAI47_20080 at 249.857 kb on + strand, within IAI47_20080 at 249.858 kb on - strand, within IAI47_20080 at 249.858 kb on - strand, within IAI47_20080 at 249.858 kb on - strand, within IAI47_20080 at 249.878 kb on + strand, within IAI47_20080 at 249.879 kb on - strand, within IAI47_20080 at 249.879 kb on - strand, within IAI47_20080 at 249.945 kb on - strand at 249.966 kb on + strand at 249.966 kb on + strand at 249.996 kb on - strand at 250.000 kb on + strand at 250.017 kb on + strand at 250.017 kb on + strand at 250.018 kb on - strand at 250.018 kb on - strand at 250.018 kb on - strand at 250.018 kb on - strand at 250.018 kb on - strand at 250.018 kb on - strand at 250.058 kb on + strand at 250.059 kb on - strand at 250.103 kb on + strand at 250.103 kb on + strand at 250.103 kb on + strand at 250.104 kb on - strand at 250.104 kb on - strand at 250.104 kb on - strand at 250.104 kb on - strand at 250.162 kb on - strand, within IAI47_20085 at 250.190 kb on - strand, within IAI47_20085 at 250.190 kb on - strand, within IAI47_20085 at 250.190 kb on - strand, within IAI47_20085 at 250.221 kb on - strand, within IAI47_20085 at 250.294 kb on + strand, within IAI47_20085 at 250.294 kb on + strand, within IAI47_20085 at 250.294 kb on + strand, within IAI47_20085 at 250.294 kb on + strand, within IAI47_20085 at 250.294 kb on + strand, within IAI47_20085 at 250.294 kb on + strand, within IAI47_20085 at 250.295 kb on - strand, within IAI47_20085 at 250.427 kb on - strand, within IAI47_20085 at 250.427 kb on - strand, within IAI47_20085 at 250.508 kb on + strand, within IAI47_20085 at 250.509 kb on - strand, within IAI47_20085 at 250.580 kb on - strand, within IAI47_20085 at 250.605 kb on - strand, within IAI47_20085 at 250.639 kb on + strand, within IAI47_20085 at 250.639 kb on + strand, within IAI47_20085 at 250.639 kb on + strand, within IAI47_20085 at 250.640 kb on - strand, within IAI47_20085 at 250.642 kb on + strand, within IAI47_20085 at 250.642 kb on + strand, within IAI47_20085 at 250.642 kb on + strand, within IAI47_20085 at 250.643 kb on - strand, within IAI47_20085 at 250.643 kb on - strand, within IAI47_20085 at 250.719 kb on + strand, within IAI47_20085 at 250.719 kb on + strand, within IAI47_20085 at 250.720 kb on - strand, within IAI47_20085 at 250.798 kb on + strand, within IAI47_20085 at 250.799 kb on - strand, within IAI47_20085 at 250.799 kb on - strand, within IAI47_20085 at 250.843 kb on + strand, within IAI47_20085 at 250.843 kb on + strand, within IAI47_20085 at 250.866 kb on - strand, within IAI47_20085 at 250.866 kb on - strand, within IAI47_20085 at 250.866 kb on - strand, within IAI47_20085 at 250.921 kb on + strand at 250.921 kb on + strand at 250.921 kb on + strand at 250.921 kb on + strand at 250.931 kb on + strand at 250.932 kb on - strand at 250.932 kb on - strand at 250.942 kb on + strand at 250.942 kb on + strand at 250.942 kb on + strand at 250.942 kb on + strand at 250.942 kb on + strand at 250.942 kb on + strand at 250.942 kb on + strand at 250.942 kb on + strand at 250.943 kb on - strand at 250.943 kb on - strand at 250.944 kb on - strand at 250.959 kb on + strand at 250.959 kb on + strand at 250.959 kb on + strand at 250.959 kb on + strand at 250.959 kb on + strand at 250.959 kb on + strand at 250.959 kb on + strand at 250.960 kb on - strand at 250.960 kb on - strand at 250.960 kb on - strand at 250.981 kb on + strand at 250.981 kb on + strand at 250.981 kb on + strand at 250.981 kb on + strand at 250.981 kb on + strand at 250.982 kb on - strand at 250.987 kb on + strand at 250.987 kb on + strand at 250.987 kb on + strand at 250.987 kb on + strand at 250.988 kb on - strand at 250.988 kb on - strand at 250.991 kb on + strand at 250.991 kb on + strand at 250.991 kb on + strand at 250.991 kb on + strand at 250.992 kb on - strand at 250.992 kb on - strand at 250.992 kb on - strand at 250.998 kb on + strand at 250.999 kb on - strand at 250.999 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 247,989 - IAI47_20075 0.63 +1.0 247,989 - IAI47_20075 0.63 -1.1 248,083 - +0.5 248,083 - IAI47_20075 0.67 +0.7 248,103 + IAI47_20075 0.68 +0.7 248,103 + IAI47_20075 0.68 +0.9 248,103 + IAI47_20075 0.68 -0.5 248,163 + IAI47_20075 0.72 +2.0 248,250 + IAI47_20075 0.76 -0.7 248,251 - IAI47_20075 0.76 -0.0 248,286 + IAI47_20075 0.78 -0.1 248,370 + IAI47_20075 0.82 +2.5 248,370 + IAI47_20075 0.82 -1.5 248,370 + IAI47_20075 0.82 +0.0 248,462 + IAI47_20075 0.87 +3.0 248,475 + IAI47_20075 0.88 +1.1 248,476 - IAI47_20075 0.88 -1.2 248,538 + +0.5 248,539 - +0.3 248,539 - +0.8 248,681 + -2.0 248,681 + -0.0 248,681 + -0.9 248,681 + -0.6 248,713 + +0.2 248,713 + +1.0 248,714 - -0.7 248,735 + +0.6 248,735 + -2.0 248,735 + -0.0 248,735 + -1.2 248,735 + +2.5 248,735 + -2.6 248,736 - +1.6 248,742 + +0.6 248,742 + -0.9 248,743 - -2.0 248,834 + -1.1 248,834 + +1.0 248,834 + +0.6 248,834 + +0.3 248,835 - +1.1 248,835 - +0.2 248,835 - -0.7 248,835 - +0.4 248,870 + -0.1 248,888 + +1.4 248,888 + -0.2 248,888 + +0.3 248,888 + +0.4 248,888 + -0.2 248,888 + -0.4 248,889 - +0.6 248,889 - -2.0 248,896 + -0.5 248,896 + +1.1 248,896 + -0.8 248,896 + +1.5 248,896 + +0.7 248,897 - +1.5 248,897 - +0.5 248,912 + +0.4 248,913 - +0.5 248,953 - +0.6 249,016 - +0.4 249,032 + -1.0 249,054 - +0.9 249,071 + IAI47_20080 0.11 +0.8 249,071 + IAI47_20080 0.11 -1.7 249,071 + IAI47_20080 0.11 +0.0 249,071 + IAI47_20080 0.11 -0.1 249,071 + IAI47_20080 0.11 +1.2 249,071 + IAI47_20080 0.11 -0.6 249,072 - IAI47_20080 0.11 +3.2 249,134 + IAI47_20080 0.17 +0.9 249,135 - IAI47_20080 0.17 +1.8 249,135 - IAI47_20080 0.17 +1.4 249,135 - IAI47_20080 0.17 -1.1 249,139 - IAI47_20080 0.17 -0.8 249,263 + IAI47_20080 0.28 -0.2 249,339 - IAI47_20080 0.35 -0.0 249,359 + IAI47_20080 0.37 -0.4 249,373 + IAI47_20080 0.39 +0.2 249,373 + IAI47_20080 0.39 -0.3 249,373 + IAI47_20080 0.39 -1.6 249,373 + IAI47_20080 0.39 -2.4 249,374 - IAI47_20080 0.39 +0.1 249,420 + IAI47_20080 0.43 +2.9 249,472 + IAI47_20080 0.48 +2.2 249,472 + IAI47_20080 0.48 -0.5 249,472 + IAI47_20080 0.48 -1.1 249,472 + IAI47_20080 0.48 +0.9 249,473 - IAI47_20080 0.48 -0.1 249,473 - IAI47_20080 0.48 +1.1 249,473 - IAI47_20080 0.48 +0.0 249,473 - IAI47_20080 0.48 +0.6 249,473 - IAI47_20080 0.48 +0.4 249,509 + IAI47_20080 0.51 +1.3 249,510 - IAI47_20080 0.51 -2.5 249,510 - IAI47_20080 0.51 -2.9 249,510 - IAI47_20080 0.51 -1.4 249,510 - IAI47_20080 0.51 +1.5 249,530 + IAI47_20080 0.53 +2.2 249,530 + IAI47_20080 0.53 +0.0 249,530 + IAI47_20080 0.53 -0.6 249,530 + IAI47_20080 0.53 +1.6 249,530 + IAI47_20080 0.53 +0.8 249,531 - IAI47_20080 0.53 -0.2 249,534 + IAI47_20080 0.53 +3.1 249,534 + IAI47_20080 0.53 +1.6 249,535 - IAI47_20080 0.54 -0.3 249,535 - IAI47_20080 0.54 +0.7 249,535 - IAI47_20080 0.54 +1.8 249,535 - IAI47_20080 0.54 -1.6 249,535 - IAI47_20080 0.54 -0.3 249,576 + IAI47_20080 0.57 +0.9 249,576 + IAI47_20080 0.57 -1.0 249,576 + IAI47_20080 0.57 -0.9 249,576 + IAI47_20080 0.57 +0.4 249,577 - IAI47_20080 0.57 +1.7 249,577 - IAI47_20080 0.57 +1.1 249,577 - IAI47_20080 0.57 -1.0 249,577 - IAI47_20080 0.57 +0.9 249,577 - IAI47_20080 0.57 +0.8 249,613 - IAI47_20080 0.61 +0.1 249,657 + IAI47_20080 0.65 +0.4 249,658 - IAI47_20080 0.65 +0.6 249,658 - IAI47_20080 0.65 +1.0 249,732 + IAI47_20080 0.72 -0.1 249,787 + IAI47_20080 0.77 +1.5 249,787 + IAI47_20080 0.77 -0.3 249,787 + IAI47_20080 0.77 +2.0 249,787 + IAI47_20080 0.77 -0.0 249,787 + IAI47_20080 0.77 +2.1 249,787 + IAI47_20080 0.77 -1.9 249,788 - IAI47_20080 0.77 -2.1 249,788 - IAI47_20080 0.77 +1.4 249,788 - IAI47_20080 0.77 -1.7 249,809 + IAI47_20080 0.79 -0.7 249,849 - IAI47_20080 0.83 +1.7 249,849 - IAI47_20080 0.83 +2.1 249,857 + IAI47_20080 0.83 +1.9 249,858 - IAI47_20080 0.83 -2.4 249,858 - IAI47_20080 0.83 +0.7 249,858 - IAI47_20080 0.83 -1.1 249,878 + IAI47_20080 0.85 +0.6 249,879 - IAI47_20080 0.85 +1.8 249,879 - IAI47_20080 0.85 -2.3 249,945 - -0.9 249,966 + -0.1 249,966 + +0.8 249,996 - +1.2 250,000 + -0.7 250,017 + +1.5 250,017 + +2.7 250,018 - -2.6 250,018 - -2.4 250,018 - +1.2 250,018 - -0.6 250,018 - +0.6 250,018 - -1.0 250,058 + -1.1 250,059 - -0.2 250,103 + +0.8 250,103 + +0.4 250,103 + +0.5 250,104 - -1.5 250,104 - +1.0 250,104 - -0.1 250,104 - -1.9 250,162 - IAI47_20085 0.13 +0.1 250,190 - IAI47_20085 0.16 +2.2 250,190 - IAI47_20085 0.16 -2.0 250,190 - IAI47_20085 0.16 -0.0 250,221 - IAI47_20085 0.19 -0.7 250,294 + IAI47_20085 0.27 +2.0 250,294 + IAI47_20085 0.27 +0.1 250,294 + IAI47_20085 0.27 -0.0 250,294 + IAI47_20085 0.27 -0.2 250,294 + IAI47_20085 0.27 -2.9 250,294 + IAI47_20085 0.27 -1.2 250,295 - IAI47_20085 0.27 +1.8 250,427 - IAI47_20085 0.41 +1.3 250,427 - IAI47_20085 0.41 +1.1 250,508 + IAI47_20085 0.49 -0.2 250,509 - IAI47_20085 0.49 +0.5 250,580 - IAI47_20085 0.57 -0.0 250,605 - IAI47_20085 0.60 +0.9 250,639 + IAI47_20085 0.63 +0.1 250,639 + IAI47_20085 0.63 -2.1 250,639 + IAI47_20085 0.63 -0.7 250,640 - IAI47_20085 0.63 +0.8 250,642 + IAI47_20085 0.64 -1.8 250,642 + IAI47_20085 0.64 +0.1 250,642 + IAI47_20085 0.64 +1.9 250,643 - IAI47_20085 0.64 +5.3 250,643 - IAI47_20085 0.64 +0.3 250,719 + IAI47_20085 0.72 -0.6 250,719 + IAI47_20085 0.72 -1.2 250,720 - IAI47_20085 0.72 +1.5 250,798 + IAI47_20085 0.80 -0.1 250,799 - IAI47_20085 0.80 +1.0 250,799 - IAI47_20085 0.80 +2.5 250,843 + IAI47_20085 0.85 -0.8 250,843 + IAI47_20085 0.85 -1.4 250,866 - IAI47_20085 0.87 +1.8 250,866 - IAI47_20085 0.87 -0.5 250,866 - IAI47_20085 0.87 -0.2 250,921 + -1.7 250,921 + -1.6 250,921 + +0.4 250,921 + +0.0 250,931 + -0.2 250,932 - +1.1 250,932 - +0.5 250,942 + +1.5 250,942 + -1.1 250,942 + +2.2 250,942 + +1.2 250,942 + -0.3 250,942 + -0.7 250,942 + -0.2 250,942 + -0.1 250,943 - -0.8 250,943 - +1.5 250,944 - +0.9 250,959 + +1.2 250,959 + +0.5 250,959 + +0.1 250,959 + +1.6 250,959 + +0.3 250,959 + +0.3 250,959 + +0.1 250,960 - +0.6 250,960 - +0.1 250,960 - +1.0 250,981 + +0.4 250,981 + -1.6 250,981 + -1.1 250,981 + +0.8 250,981 + -1.0 250,982 - -0.7 250,987 + +0.2 250,987 + +0.8 250,987 + +0.2 250,987 + -2.1 250,988 - +0.5 250,988 - -0.1 250,991 + -0.6 250,991 + -0.2 250,991 + -2.0 250,991 + +2.8 250,992 - -2.1 250,992 - +1.5 250,992 - -0.0 250,998 + +0.9 250,999 - -0.5 250,999 - -0.8
Or see this region's nucleotide sequence