Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19585 and IAI47_19590 are separated by 33 nucleotides IAI47_19590 and IAI47_19595 are separated by 23 nucleotides IAI47_19595 and IAI47_19600 overlap by 4 nucleotides IAI47_19600 and IAI47_19605 overlap by 4 nucleotides
IAI47_19585: IAI47_19585 - EAL domain-containing protein, at 146,121 to 147,893
_19585
IAI47_19590: IAI47_19590 - ABC transporter substrate-binding protein, at 147,927 to 148,862
_19590
IAI47_19595: IAI47_19595 - ABC transporter permease, at 148,886 to 149,641
_19595
IAI47_19600: IAI47_19600 - ABC transporter ATP-binding protein, at 149,638 to 150,378
_19600
IAI47_19605: IAI47_19605 - thiaminase II, at 150,375 to 151,076
_19605
Position (kb) 148
149
150 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 147.892 kb on - strand at 147.895 kb on - strand at 147.895 kb on - strand at 147.925 kb on + strand at 147.926 kb on - strand at 147.946 kb on + strand at 147.972 kb on - strand at 147.972 kb on - strand at 147.972 kb on - strand at 147.972 kb on - strand at 147.972 kb on - strand at 147.972 kb on - strand at 147.984 kb on - strand at 147.984 kb on - strand at 147.984 kb on - strand at 148.003 kb on + strand at 148.003 kb on + strand at 148.004 kb on - strand at 148.004 kb on - strand at 148.004 kb on - strand at 148.004 kb on - strand at 148.004 kb on - strand at 148.091 kb on + strand, within IAI47_19590 at 148.092 kb on - strand, within IAI47_19590 at 148.243 kb on + strand, within IAI47_19590 at 148.244 kb on - strand, within IAI47_19590 at 148.258 kb on + strand, within IAI47_19590 at 148.258 kb on + strand, within IAI47_19590 at 148.258 kb on + strand, within IAI47_19590 at 148.259 kb on - strand, within IAI47_19590 at 148.259 kb on - strand, within IAI47_19590 at 148.259 kb on - strand, within IAI47_19590 at 148.259 kb on - strand, within IAI47_19590 at 148.271 kb on + strand, within IAI47_19590 at 148.347 kb on + strand, within IAI47_19590 at 148.347 kb on + strand, within IAI47_19590 at 148.386 kb on - strand, within IAI47_19590 at 148.386 kb on - strand, within IAI47_19590 at 148.460 kb on + strand, within IAI47_19590 at 148.461 kb on - strand, within IAI47_19590 at 148.461 kb on - strand, within IAI47_19590 at 148.461 kb on - strand, within IAI47_19590 at 148.494 kb on + strand, within IAI47_19590 at 148.495 kb on - strand, within IAI47_19590 at 148.524 kb on + strand, within IAI47_19590 at 148.525 kb on - strand, within IAI47_19590 at 148.525 kb on - strand, within IAI47_19590 at 148.525 kb on - strand, within IAI47_19590 at 148.525 kb on - strand, within IAI47_19590 at 148.618 kb on + strand, within IAI47_19590 at 148.618 kb on + strand, within IAI47_19590 at 148.619 kb on - strand, within IAI47_19590 at 148.619 kb on - strand, within IAI47_19590 at 148.622 kb on + strand, within IAI47_19590 at 148.622 kb on + strand, within IAI47_19590 at 148.622 kb on + strand, within IAI47_19590 at 148.622 kb on + strand, within IAI47_19590 at 148.622 kb on + strand, within IAI47_19590 at 148.622 kb on + strand, within IAI47_19590 at 148.622 kb on + strand, within IAI47_19590 at 148.622 kb on + strand, within IAI47_19590 at 148.622 kb on + strand, within IAI47_19590 at 148.623 kb on - strand, within IAI47_19590 at 148.623 kb on - strand, within IAI47_19590 at 148.623 kb on - strand, within IAI47_19590 at 148.623 kb on - strand, within IAI47_19590 at 148.623 kb on - strand, within IAI47_19590 at 148.623 kb on - strand, within IAI47_19590 at 148.623 kb on - strand, within IAI47_19590 at 148.629 kb on + strand, within IAI47_19590 at 148.629 kb on + strand, within IAI47_19590 at 148.629 kb on + strand, within IAI47_19590 at 148.630 kb on - strand, within IAI47_19590 at 148.630 kb on - strand, within IAI47_19590 at 148.691 kb on + strand, within IAI47_19590 at 148.691 kb on + strand, within IAI47_19590 at 148.692 kb on - strand, within IAI47_19590 at 148.692 kb on - strand, within IAI47_19590 at 148.692 kb on - strand, within IAI47_19590 at 148.692 kb on - strand, within IAI47_19590 at 148.695 kb on + strand, within IAI47_19590 at 148.695 kb on + strand, within IAI47_19590 at 148.695 kb on + strand, within IAI47_19590 at 148.695 kb on + strand, within IAI47_19590 at 148.696 kb on - strand, within IAI47_19590 at 148.696 kb on - strand, within IAI47_19590 at 148.742 kb on + strand, within IAI47_19590 at 148.742 kb on + strand, within IAI47_19590 at 148.743 kb on - strand, within IAI47_19590 at 148.743 kb on - strand, within IAI47_19590 at 148.743 kb on - strand, within IAI47_19590 at 148.743 kb on - strand, within IAI47_19590 at 148.766 kb on + strand, within IAI47_19590 at 148.766 kb on + strand, within IAI47_19590 at 148.767 kb on - strand, within IAI47_19590 at 148.767 kb on - strand, within IAI47_19590 at 148.767 kb on - strand, within IAI47_19590 at 148.767 kb on - strand, within IAI47_19590 at 148.768 kb on + strand, within IAI47_19590 at 148.768 kb on + strand, within IAI47_19590 at 148.768 kb on + strand, within IAI47_19590 at 148.769 kb on - strand at 148.842 kb on + strand at 148.842 kb on + strand at 148.856 kb on + strand at 148.857 kb on - strand at 148.857 kb on - strand at 148.857 kb on - strand at 148.878 kb on - strand at 148.909 kb on + strand at 148.909 kb on + strand at 148.909 kb on + strand at 148.909 kb on + strand at 148.909 kb on + strand at 148.910 kb on - strand at 148.910 kb on - strand at 148.910 kb on - strand at 148.910 kb on - strand at 149.069 kb on - strand, within IAI47_19595 at 149.179 kb on + strand, within IAI47_19595 at 149.180 kb on - strand, within IAI47_19595 at 149.333 kb on + strand, within IAI47_19595 at 149.381 kb on + strand, within IAI47_19595 at 149.382 kb on - strand, within IAI47_19595 at 149.394 kb on + strand, within IAI47_19595 at 149.394 kb on + strand, within IAI47_19595 at 149.394 kb on + strand, within IAI47_19595 at 149.394 kb on + strand, within IAI47_19595 at 149.394 kb on + strand, within IAI47_19595 at 149.394 kb on + strand, within IAI47_19595 at 149.394 kb on + strand, within IAI47_19595 at 149.394 kb on + strand, within IAI47_19595 at 149.395 kb on - strand, within IAI47_19595 at 149.395 kb on - strand, within IAI47_19595 at 149.481 kb on + strand, within IAI47_19595 at 149.481 kb on + strand, within IAI47_19595 at 149.481 kb on + strand, within IAI47_19595 at 149.481 kb on + strand, within IAI47_19595 at 149.482 kb on - strand, within IAI47_19595 at 149.482 kb on - strand, within IAI47_19595 at 149.490 kb on + strand, within IAI47_19595 at 149.490 kb on + strand, within IAI47_19595 at 149.490 kb on + strand, within IAI47_19595 at 149.491 kb on - strand, within IAI47_19595 at 149.491 kb on - strand, within IAI47_19595 at 149.515 kb on + strand, within IAI47_19595 at 149.515 kb on + strand, within IAI47_19595 at 149.516 kb on - strand, within IAI47_19595 at 149.516 kb on - strand, within IAI47_19595 at 149.554 kb on + strand, within IAI47_19595 at 149.554 kb on + strand, within IAI47_19595 at 149.554 kb on + strand, within IAI47_19595 at 149.555 kb on - strand, within IAI47_19595 at 149.605 kb on + strand at 149.605 kb on + strand at 149.605 kb on + strand at 149.606 kb on - strand at 149.606 kb on - strand at 149.606 kb on - strand at 149.656 kb on + strand at 149.656 kb on + strand at 149.656 kb on + strand at 149.657 kb on - strand at 149.657 kb on - strand at 149.686 kb on + strand at 149.686 kb on + strand at 149.686 kb on + strand at 149.686 kb on + strand at 149.687 kb on - strand at 149.687 kb on - strand at 149.689 kb on + strand at 149.728 kb on + strand, within IAI47_19600 at 149.728 kb on + strand, within IAI47_19600 at 149.930 kb on + strand, within IAI47_19600 at 149.930 kb on + strand, within IAI47_19600 at 149.930 kb on + strand, within IAI47_19600 at 149.930 kb on + strand, within IAI47_19600 at 149.931 kb on - strand, within IAI47_19600 at 150.029 kb on + strand, within IAI47_19600 at 150.174 kb on + strand, within IAI47_19600 at 150.198 kb on + strand, within IAI47_19600 at 150.199 kb on - strand, within IAI47_19600 at 150.199 kb on - strand, within IAI47_19600 at 150.321 kb on + strand at 150.321 kb on + strand at 150.321 kb on + strand at 150.322 kb on - strand at 150.345 kb on + strand at 150.346 kb on - strand at 150.408 kb on + strand at 150.520 kb on - strand, within IAI47_19605 at 150.534 kb on + strand, within IAI47_19605 at 150.547 kb on + strand, within IAI47_19605 at 150.548 kb on - strand, within IAI47_19605 at 150.548 kb on - strand, within IAI47_19605 at 150.584 kb on - strand, within IAI47_19605 at 150.584 kb on - strand, within IAI47_19605
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 147,892 - +0.2 147,895 - +0.3 147,895 - +0.4 147,925 + +2.2 147,926 - +0.6 147,946 + -0.2 147,972 - -0.1 147,972 - +0.3 147,972 - -2.5 147,972 - -1.1 147,972 - -0.2 147,972 - -2.1 147,984 - +1.1 147,984 - +0.6 147,984 - +1.2 148,003 + +0.4 148,003 + -0.1 148,004 - +2.2 148,004 - +2.5 148,004 - -0.2 148,004 - -1.7 148,004 - +0.1 148,091 + IAI47_19590 0.18 +1.5 148,092 - IAI47_19590 0.18 -0.4 148,243 + IAI47_19590 0.34 -0.2 148,244 - IAI47_19590 0.34 +0.1 148,258 + IAI47_19590 0.35 +1.1 148,258 + IAI47_19590 0.35 +0.2 148,258 + IAI47_19590 0.35 -0.9 148,259 - IAI47_19590 0.35 -1.8 148,259 - IAI47_19590 0.35 +1.1 148,259 - IAI47_19590 0.35 -1.8 148,259 - IAI47_19590 0.35 +0.2 148,271 + IAI47_19590 0.37 +1.1 148,347 + IAI47_19590 0.45 -0.1 148,347 + IAI47_19590 0.45 -0.1 148,386 - IAI47_19590 0.49 +0.0 148,386 - IAI47_19590 0.49 -2.4 148,460 + IAI47_19590 0.57 +0.1 148,461 - IAI47_19590 0.57 -0.3 148,461 - IAI47_19590 0.57 +0.3 148,461 - IAI47_19590 0.57 +0.0 148,494 + IAI47_19590 0.61 -0.5 148,495 - IAI47_19590 0.61 -1.1 148,524 + IAI47_19590 0.64 +1.7 148,525 - IAI47_19590 0.64 -1.8 148,525 - IAI47_19590 0.64 -0.8 148,525 - IAI47_19590 0.64 -0.1 148,525 - IAI47_19590 0.64 -1.1 148,618 + IAI47_19590 0.74 +0.2 148,618 + IAI47_19590 0.74 -0.2 148,619 - IAI47_19590 0.74 +0.6 148,619 - IAI47_19590 0.74 +0.6 148,622 + IAI47_19590 0.74 -1.2 148,622 + IAI47_19590 0.74 -1.8 148,622 + IAI47_19590 0.74 -1.9 148,622 + IAI47_19590 0.74 +1.7 148,622 + IAI47_19590 0.74 -0.5 148,622 + IAI47_19590 0.74 -0.7 148,622 + IAI47_19590 0.74 -1.5 148,622 + IAI47_19590 0.74 -1.7 148,622 + IAI47_19590 0.74 -0.3 148,623 - IAI47_19590 0.74 -0.5 148,623 - IAI47_19590 0.74 -2.7 148,623 - IAI47_19590 0.74 -0.2 148,623 - IAI47_19590 0.74 -1.5 148,623 - IAI47_19590 0.74 +0.3 148,623 - IAI47_19590 0.74 -0.3 148,623 - IAI47_19590 0.74 +0.5 148,629 + IAI47_19590 0.75 -1.1 148,629 + IAI47_19590 0.75 +2.5 148,629 + IAI47_19590 0.75 -0.8 148,630 - IAI47_19590 0.75 -0.9 148,630 - IAI47_19590 0.75 -0.0 148,691 + IAI47_19590 0.82 -0.2 148,691 + IAI47_19590 0.82 -1.4 148,692 - IAI47_19590 0.82 +0.2 148,692 - IAI47_19590 0.82 +0.1 148,692 - IAI47_19590 0.82 +0.3 148,692 - IAI47_19590 0.82 +0.9 148,695 + IAI47_19590 0.82 -1.7 148,695 + IAI47_19590 0.82 -2.9 148,695 + IAI47_19590 0.82 -1.0 148,695 + IAI47_19590 0.82 +0.1 148,696 - IAI47_19590 0.82 +2.4 148,696 - IAI47_19590 0.82 +0.4 148,742 + IAI47_19590 0.87 +1.2 148,742 + IAI47_19590 0.87 +0.2 148,743 - IAI47_19590 0.87 -1.8 148,743 - IAI47_19590 0.87 -0.7 148,743 - IAI47_19590 0.87 -0.5 148,743 - IAI47_19590 0.87 +0.8 148,766 + IAI47_19590 0.90 +1.8 148,766 + IAI47_19590 0.90 -0.1 148,767 - IAI47_19590 0.90 -0.3 148,767 - IAI47_19590 0.90 +1.5 148,767 - IAI47_19590 0.90 -0.9 148,767 - IAI47_19590 0.90 +0.2 148,768 + IAI47_19590 0.90 +2.3 148,768 + IAI47_19590 0.90 +1.4 148,768 + IAI47_19590 0.90 +0.5 148,769 - +0.1 148,842 + -0.2 148,842 + -2.1 148,856 + -0.6 148,857 - +0.8 148,857 - +0.5 148,857 - -0.7 148,878 - +1.3 148,909 + -0.3 148,909 + +0.7 148,909 + -0.5 148,909 + -1.8 148,909 + +1.7 148,910 - +0.4 148,910 - +2.5 148,910 - -0.3 148,910 - +0.1 149,069 - IAI47_19595 0.24 +0.7 149,179 + IAI47_19595 0.39 +1.4 149,180 - IAI47_19595 0.39 +0.3 149,333 + IAI47_19595 0.59 +0.6 149,381 + IAI47_19595 0.65 +3.5 149,382 - IAI47_19595 0.66 +3.5 149,394 + IAI47_19595 0.67 -0.4 149,394 + IAI47_19595 0.67 -0.6 149,394 + IAI47_19595 0.67 +1.2 149,394 + IAI47_19595 0.67 -1.0 149,394 + IAI47_19595 0.67 -0.4 149,394 + IAI47_19595 0.67 +0.4 149,394 + IAI47_19595 0.67 +0.7 149,394 + IAI47_19595 0.67 -0.2 149,395 - IAI47_19595 0.67 +0.0 149,395 - IAI47_19595 0.67 +0.2 149,481 + IAI47_19595 0.79 +0.1 149,481 + IAI47_19595 0.79 +1.1 149,481 + IAI47_19595 0.79 -1.3 149,481 + IAI47_19595 0.79 +0.5 149,482 - IAI47_19595 0.79 +0.7 149,482 - IAI47_19595 0.79 +1.3 149,490 + IAI47_19595 0.80 -0.7 149,490 + IAI47_19595 0.80 -0.1 149,490 + IAI47_19595 0.80 +0.4 149,491 - IAI47_19595 0.80 +1.9 149,491 - IAI47_19595 0.80 +0.6 149,515 + IAI47_19595 0.83 -1.6 149,515 + IAI47_19595 0.83 -0.5 149,516 - IAI47_19595 0.83 +1.5 149,516 - IAI47_19595 0.83 +0.5 149,554 + IAI47_19595 0.88 -1.3 149,554 + IAI47_19595 0.88 -0.4 149,554 + IAI47_19595 0.88 -0.2 149,555 - IAI47_19595 0.88 +0.7 149,605 + +1.5 149,605 + +1.7 149,605 + +0.9 149,606 - -2.0 149,606 - -0.1 149,606 - +0.8 149,656 + -0.8 149,656 + -2.8 149,656 + +0.0 149,657 - +3.1 149,657 - +1.0 149,686 + +0.8 149,686 + -0.2 149,686 + +0.6 149,686 + +1.9 149,687 - -0.3 149,687 - +2.3 149,689 + -0.4 149,728 + IAI47_19600 0.12 -1.5 149,728 + IAI47_19600 0.12 -1.4 149,930 + IAI47_19600 0.39 -1.1 149,930 + IAI47_19600 0.39 -0.3 149,930 + IAI47_19600 0.39 -1.1 149,930 + IAI47_19600 0.39 +1.2 149,931 - IAI47_19600 0.40 -0.4 150,029 + IAI47_19600 0.53 -1.0 150,174 + IAI47_19600 0.72 +3.8 150,198 + IAI47_19600 0.76 +0.4 150,199 - IAI47_19600 0.76 +0.4 150,199 - IAI47_19600 0.76 -2.0 150,321 + +0.8 150,321 + +1.1 150,321 + +0.3 150,322 - +0.6 150,345 + -0.5 150,346 - -1.7 150,408 + -0.9 150,520 - IAI47_19605 0.21 +1.9 150,534 + IAI47_19605 0.23 +1.4 150,547 + IAI47_19605 0.25 -0.2 150,548 - IAI47_19605 0.25 +1.3 150,548 - IAI47_19605 0.25 -1.1 150,584 - IAI47_19605 0.30 -1.1 150,584 - IAI47_19605 0.30 -0.7
Or see this region's nucleotide sequence