Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_07805 and IAI47_07810 are separated by 95 nucleotides IAI47_07810 and IAI47_07815 overlap by 4 nucleotides IAI47_07815 and IAI47_07820 overlap by 47 nucleotides
IAI47_07805: IAI47_07805 - phosphonate ABC transporter substrate-binding protein, at 1,651,693 to 1,652,619
_07805
IAI47_07810: IAI47_07810 - phosphonate ABC transporter, permease protein PhnE, at 1,652,715 to 1,653,575
_07810
IAI47_07815: IAI47_07815 - phosphonate ABC transporter, permease protein PhnE, at 1,653,572 to 1,654,456
_07815
IAI47_07820: IAI47_07820 - response regulator, at 1,654,410 to 1,657,028
_07820
Position (kb)
1653
1654
1655 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1652.584 kb on - strand at 1652.733 kb on + strand at 1652.733 kb on + strand at 1652.733 kb on + strand at 1652.734 kb on - strand at 1652.734 kb on - strand at 1652.734 kb on - strand at 1652.734 kb on - strand at 1652.734 kb on - strand at 1652.734 kb on - strand at 1652.734 kb on - strand at 1652.736 kb on + strand at 1652.736 kb on + strand at 1652.736 kb on + strand at 1652.736 kb on + strand at 1652.736 kb on + strand at 1652.736 kb on + strand at 1652.737 kb on - strand at 1652.737 kb on - strand at 1652.737 kb on - strand at 1652.737 kb on - strand at 1652.737 kb on - strand at 1652.802 kb on + strand, within IAI47_07810 at 1652.802 kb on + strand, within IAI47_07810 at 1652.802 kb on + strand, within IAI47_07810 at 1652.802 kb on + strand, within IAI47_07810 at 1652.803 kb on - strand, within IAI47_07810 at 1652.875 kb on - strand, within IAI47_07810 at 1652.875 kb on - strand, within IAI47_07810 at 1652.949 kb on + strand, within IAI47_07810 at 1652.949 kb on + strand, within IAI47_07810 at 1653.243 kb on + strand, within IAI47_07810 at 1653.244 kb on - strand, within IAI47_07810 at 1653.244 kb on - strand, within IAI47_07810 at 1653.363 kb on + strand, within IAI47_07810 at 1653.363 kb on + strand, within IAI47_07810 at 1653.364 kb on - strand, within IAI47_07810 at 1653.364 kb on - strand, within IAI47_07810 at 1653.364 kb on - strand, within IAI47_07810 at 1653.364 kb on - strand, within IAI47_07810 at 1653.455 kb on + strand, within IAI47_07810 at 1653.455 kb on + strand, within IAI47_07810 at 1653.486 kb on - strand, within IAI47_07810 at 1653.514 kb on + strand at 1653.514 kb on + strand at 1653.515 kb on - strand at 1653.515 kb on - strand at 1653.515 kb on - strand at 1653.528 kb on - strand at 1653.528 kb on - strand at 1653.533 kb on - strand at 1653.533 kb on - strand at 1653.585 kb on + strand at 1653.585 kb on + strand at 1653.585 kb on + strand at 1653.645 kb on - strand at 1653.657 kb on + strand at 1653.657 kb on + strand at 1653.657 kb on + strand at 1653.657 kb on + strand at 1653.658 kb on - strand at 1653.686 kb on + strand, within IAI47_07815 at 1653.686 kb on + strand, within IAI47_07815 at 1653.687 kb on - strand, within IAI47_07815 at 1653.687 kb on - strand, within IAI47_07815 at 1653.687 kb on - strand, within IAI47_07815 at 1653.687 kb on - strand, within IAI47_07815 at 1653.687 kb on - strand, within IAI47_07815 at 1653.687 kb on - strand, within IAI47_07815 at 1653.687 kb on - strand, within IAI47_07815 at 1653.687 kb on - strand, within IAI47_07815 at 1653.687 kb on - strand, within IAI47_07815 at 1653.689 kb on + strand, within IAI47_07815 at 1653.689 kb on + strand, within IAI47_07815 at 1653.689 kb on + strand, within IAI47_07815 at 1653.689 kb on + strand, within IAI47_07815 at 1653.689 kb on + strand, within IAI47_07815 at 1653.689 kb on + strand, within IAI47_07815 at 1653.689 kb on + strand, within IAI47_07815 at 1653.689 kb on + strand, within IAI47_07815 at 1653.689 kb on + strand, within IAI47_07815 at 1653.690 kb on - strand, within IAI47_07815 at 1653.690 kb on - strand, within IAI47_07815 at 1653.690 kb on - strand, within IAI47_07815 at 1653.690 kb on - strand, within IAI47_07815 at 1653.708 kb on + strand, within IAI47_07815 at 1653.708 kb on + strand, within IAI47_07815 at 1653.709 kb on - strand, within IAI47_07815 at 1653.709 kb on - strand, within IAI47_07815 at 1653.761 kb on + strand, within IAI47_07815 at 1653.762 kb on - strand, within IAI47_07815 at 1653.762 kb on - strand, within IAI47_07815 at 1653.762 kb on - strand, within IAI47_07815 at 1653.840 kb on + strand, within IAI47_07815 at 1653.840 kb on + strand, within IAI47_07815 at 1653.840 kb on + strand, within IAI47_07815 at 1653.841 kb on - strand, within IAI47_07815 at 1653.975 kb on + strand, within IAI47_07815 at 1653.975 kb on + strand, within IAI47_07815 at 1654.008 kb on + strand, within IAI47_07815 at 1654.009 kb on - strand, within IAI47_07815 at 1654.009 kb on - strand, within IAI47_07815 at 1654.058 kb on - strand, within IAI47_07815 at 1654.223 kb on + strand, within IAI47_07815 at 1654.240 kb on + strand, within IAI47_07815 at 1654.295 kb on + strand, within IAI47_07815 at 1654.296 kb on - strand, within IAI47_07815 at 1654.296 kb on - strand, within IAI47_07815 at 1654.406 kb on + strand at 1654.406 kb on + strand at 1654.406 kb on + strand at 1654.406 kb on + strand at 1654.407 kb on - strand at 1654.407 kb on - strand at 1654.407 kb on - strand at 1654.485 kb on + strand at 1654.486 kb on - strand at 1654.486 kb on - strand at 1654.486 kb on - strand at 1654.580 kb on + strand at 1654.581 kb on - strand at 1654.641 kb on - strand at 1654.653 kb on + strand at 1654.745 kb on - strand, within IAI47_07820 at 1654.956 kb on + strand, within IAI47_07820 at 1654.956 kb on + strand, within IAI47_07820 at 1654.957 kb on - strand, within IAI47_07820 at 1654.957 kb on - strand, within IAI47_07820 at 1654.957 kb on - strand, within IAI47_07820 at 1654.957 kb on - strand, within IAI47_07820 at 1654.957 kb on - strand, within IAI47_07820 at 1654.957 kb on - strand, within IAI47_07820 at 1654.957 kb on - strand, within IAI47_07820 at 1654.987 kb on + strand, within IAI47_07820 at 1654.988 kb on - strand, within IAI47_07820 at 1655.283 kb on - strand, within IAI47_07820 at 1655.369 kb on - strand, within IAI47_07820
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,652,584 - +0.1 1,652,733 + +0.9 1,652,733 + -0.6 1,652,733 + +1.4 1,652,734 - +0.6 1,652,734 - +1.5 1,652,734 - +1.6 1,652,734 - +0.9 1,652,734 - +2.7 1,652,734 - -0.6 1,652,734 - -0.3 1,652,736 + -0.7 1,652,736 + -0.3 1,652,736 + -1.8 1,652,736 + -0.8 1,652,736 + +1.5 1,652,736 + +0.9 1,652,737 - +0.5 1,652,737 - +0.7 1,652,737 - -1.2 1,652,737 - +1.1 1,652,737 - -0.9 1,652,802 + IAI47_07810 0.10 +1.4 1,652,802 + IAI47_07810 0.10 +0.6 1,652,802 + IAI47_07810 0.10 +1.6 1,652,802 + IAI47_07810 0.10 -0.1 1,652,803 - IAI47_07810 0.10 -0.1 1,652,875 - IAI47_07810 0.19 +1.4 1,652,875 - IAI47_07810 0.19 -1.4 1,652,949 + IAI47_07810 0.27 +0.2 1,652,949 + IAI47_07810 0.27 +0.7 1,653,243 + IAI47_07810 0.61 -1.0 1,653,244 - IAI47_07810 0.61 -0.2 1,653,244 - IAI47_07810 0.61 +2.2 1,653,363 + IAI47_07810 0.75 +0.3 1,653,363 + IAI47_07810 0.75 -1.0 1,653,364 - IAI47_07810 0.75 +0.1 1,653,364 - IAI47_07810 0.75 +0.8 1,653,364 - IAI47_07810 0.75 -0.6 1,653,364 - IAI47_07810 0.75 +1.9 1,653,455 + IAI47_07810 0.86 +1.9 1,653,455 + IAI47_07810 0.86 +0.3 1,653,486 - IAI47_07810 0.90 -0.2 1,653,514 + -0.6 1,653,514 + +2.1 1,653,515 - +0.7 1,653,515 - +0.0 1,653,515 - -0.3 1,653,528 - -0.3 1,653,528 - -2.8 1,653,533 - -2.8 1,653,533 - +0.2 1,653,585 + +0.7 1,653,585 + -0.1 1,653,585 + -1.1 1,653,645 - -1.5 1,653,657 + +1.0 1,653,657 + -1.6 1,653,657 + -0.3 1,653,657 + +1.5 1,653,658 - -1.1 1,653,686 + IAI47_07815 0.13 +0.5 1,653,686 + IAI47_07815 0.13 -2.0 1,653,687 - IAI47_07815 0.13 +0.0 1,653,687 - IAI47_07815 0.13 +0.3 1,653,687 - IAI47_07815 0.13 +0.0 1,653,687 - IAI47_07815 0.13 -1.3 1,653,687 - IAI47_07815 0.13 +0.7 1,653,687 - IAI47_07815 0.13 -1.9 1,653,687 - IAI47_07815 0.13 -0.0 1,653,687 - IAI47_07815 0.13 -0.1 1,653,687 - IAI47_07815 0.13 +1.8 1,653,689 + IAI47_07815 0.13 -0.3 1,653,689 + IAI47_07815 0.13 +0.5 1,653,689 + IAI47_07815 0.13 -2.1 1,653,689 + IAI47_07815 0.13 +1.1 1,653,689 + IAI47_07815 0.13 +2.0 1,653,689 + IAI47_07815 0.13 -1.1 1,653,689 + IAI47_07815 0.13 -2.8 1,653,689 + IAI47_07815 0.13 +0.7 1,653,689 + IAI47_07815 0.13 -1.2 1,653,690 - IAI47_07815 0.13 +0.3 1,653,690 - IAI47_07815 0.13 -0.1 1,653,690 - IAI47_07815 0.13 -1.5 1,653,690 - IAI47_07815 0.13 +0.2 1,653,708 + IAI47_07815 0.15 -0.7 1,653,708 + IAI47_07815 0.15 -0.7 1,653,709 - IAI47_07815 0.15 +0.8 1,653,709 - IAI47_07815 0.15 +0.4 1,653,761 + IAI47_07815 0.21 +2.3 1,653,762 - IAI47_07815 0.21 +0.0 1,653,762 - IAI47_07815 0.21 +0.4 1,653,762 - IAI47_07815 0.21 +2.7 1,653,840 + IAI47_07815 0.30 +0.3 1,653,840 + IAI47_07815 0.30 +0.6 1,653,840 + IAI47_07815 0.30 +0.7 1,653,841 - IAI47_07815 0.30 -0.3 1,653,975 + IAI47_07815 0.46 -0.8 1,653,975 + IAI47_07815 0.46 -1.5 1,654,008 + IAI47_07815 0.49 +0.6 1,654,009 - IAI47_07815 0.49 +1.2 1,654,009 - IAI47_07815 0.49 -0.1 1,654,058 - IAI47_07815 0.55 +0.3 1,654,223 + IAI47_07815 0.74 +1.4 1,654,240 + IAI47_07815 0.75 +1.5 1,654,295 + IAI47_07815 0.82 -1.8 1,654,296 - IAI47_07815 0.82 -1.0 1,654,296 - IAI47_07815 0.82 -0.5 1,654,406 + -1.1 1,654,406 + +0.1 1,654,406 + -0.0 1,654,406 + +0.8 1,654,407 - -1.6 1,654,407 - +0.1 1,654,407 - -1.0 1,654,485 + -1.0 1,654,486 - +0.1 1,654,486 - +0.6 1,654,486 - -0.0 1,654,580 + -0.4 1,654,581 - +3.1 1,654,641 - +0.2 1,654,653 + +0.4 1,654,745 - IAI47_07820 0.13 -0.8 1,654,956 + IAI47_07820 0.21 +0.5 1,654,956 + IAI47_07820 0.21 -1.3 1,654,957 - IAI47_07820 0.21 -1.2 1,654,957 - IAI47_07820 0.21 +0.1 1,654,957 - IAI47_07820 0.21 -2.4 1,654,957 - IAI47_07820 0.21 +0.7 1,654,957 - IAI47_07820 0.21 +1.7 1,654,957 - IAI47_07820 0.21 +0.8 1,654,957 - IAI47_07820 0.21 -3.1 1,654,987 + IAI47_07820 0.22 -1.7 1,654,988 - IAI47_07820 0.22 -1.9 1,655,283 - IAI47_07820 0.33 +0.1 1,655,369 - IAI47_07820 0.37 -0.8
Or see this region's nucleotide sequence