Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_06240 and IAI47_06245 are separated by 2 nucleotides IAI47_06245 and IAI47_06250 are separated by 29 nucleotides IAI47_06250 and IAI47_06255 are separated by 144 nucleotides
IAI47_06240: IAI47_06240 - aspartate-semialdehyde dehydrogenase, at 1,269,293 to 1,270,300
_06240
IAI47_06245: IAI47_06245 - tRNA pseudouridine(38-40) synthase TruA, at 1,270,303 to 1,271,106
_06245
IAI47_06250: IAI47_06250 - DedA family protein, at 1,271,136 to 1,271,798
_06250
IAI47_06255: IAI47_06255 - acetyl-CoA carboxylase, carboxyltransferase subunit beta, at 1,271,943 to 1,272,857
_06255
Position (kb)
1271
1272 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1270.183 kb on + strand, within IAI47_06240 at 1270.183 kb on + strand, within IAI47_06240 at 1270.184 kb on - strand, within IAI47_06240 at 1270.190 kb on + strand, within IAI47_06240 at 1270.190 kb on + strand, within IAI47_06240 at 1270.191 kb on - strand, within IAI47_06240 at 1270.196 kb on - strand, within IAI47_06240 at 1270.206 kb on + strand at 1270.206 kb on + strand at 1270.234 kb on + strand at 1270.292 kb on + strand at 1270.292 kb on + strand at 1270.292 kb on + strand at 1270.345 kb on + strand at 1270.364 kb on - strand at 1270.409 kb on + strand, within IAI47_06245 at 1270.651 kb on + strand, within IAI47_06245 at 1270.652 kb on - strand, within IAI47_06245 at 1270.660 kb on + strand, within IAI47_06245 at 1270.661 kb on - strand, within IAI47_06245 at 1270.712 kb on - strand, within IAI47_06245 at 1270.712 kb on - strand, within IAI47_06245 at 1270.712 kb on - strand, within IAI47_06245 at 1270.858 kb on + strand, within IAI47_06245 at 1270.858 kb on + strand, within IAI47_06245 at 1270.859 kb on - strand, within IAI47_06245 at 1270.934 kb on + strand, within IAI47_06245 at 1270.934 kb on + strand, within IAI47_06245 at 1270.935 kb on - strand, within IAI47_06245 at 1270.947 kb on + strand, within IAI47_06245 at 1271.030 kb on - strand at 1271.047 kb on + strand at 1271.104 kb on + strand at 1271.104 kb on + strand at 1271.104 kb on + strand at 1271.109 kb on + strand at 1271.122 kb on + strand at 1271.123 kb on - strand at 1271.123 kb on - strand at 1271.123 kb on - strand at 1271.165 kb on + strand at 1271.166 kb on - strand at 1271.208 kb on + strand, within IAI47_06250 at 1271.208 kb on + strand, within IAI47_06250 at 1271.208 kb on + strand, within IAI47_06250 at 1271.209 kb on - strand, within IAI47_06250 at 1271.214 kb on - strand, within IAI47_06250 at 1271.214 kb on - strand, within IAI47_06250 at 1271.223 kb on + strand, within IAI47_06250 at 1271.223 kb on + strand, within IAI47_06250 at 1271.223 kb on + strand, within IAI47_06250 at 1271.224 kb on - strand, within IAI47_06250 at 1271.228 kb on + strand, within IAI47_06250 at 1271.229 kb on - strand, within IAI47_06250 at 1271.333 kb on + strand, within IAI47_06250 at 1271.333 kb on + strand, within IAI47_06250 at 1271.334 kb on - strand, within IAI47_06250 at 1271.354 kb on + strand, within IAI47_06250 at 1271.354 kb on + strand, within IAI47_06250 at 1271.354 kb on + strand, within IAI47_06250 at 1271.354 kb on + strand, within IAI47_06250 at 1271.354 kb on + strand, within IAI47_06250 at 1271.354 kb on + strand, within IAI47_06250 at 1271.354 kb on + strand, within IAI47_06250 at 1271.354 kb on + strand, within IAI47_06250 at 1271.355 kb on - strand, within IAI47_06250 at 1271.355 kb on - strand, within IAI47_06250 at 1271.355 kb on - strand, within IAI47_06250 at 1271.355 kb on - strand, within IAI47_06250 at 1271.355 kb on - strand, within IAI47_06250 at 1271.355 kb on - strand, within IAI47_06250 at 1271.355 kb on - strand, within IAI47_06250 at 1271.355 kb on - strand, within IAI47_06250 at 1271.368 kb on + strand, within IAI47_06250 at 1271.403 kb on + strand, within IAI47_06250 at 1271.403 kb on + strand, within IAI47_06250 at 1271.403 kb on + strand, within IAI47_06250 at 1271.403 kb on + strand, within IAI47_06250 at 1271.403 kb on + strand, within IAI47_06250 at 1271.496 kb on + strand, within IAI47_06250 at 1271.522 kb on + strand, within IAI47_06250 at 1271.523 kb on - strand, within IAI47_06250 at 1271.585 kb on + strand, within IAI47_06250 at 1271.585 kb on + strand, within IAI47_06250 at 1271.585 kb on + strand, within IAI47_06250 at 1271.585 kb on + strand, within IAI47_06250 at 1271.585 kb on + strand, within IAI47_06250 at 1271.585 kb on + strand, within IAI47_06250 at 1271.585 kb on + strand, within IAI47_06250 at 1271.585 kb on + strand, within IAI47_06250 at 1271.586 kb on - strand, within IAI47_06250 at 1271.586 kb on - strand, within IAI47_06250 at 1271.586 kb on - strand, within IAI47_06250 at 1271.586 kb on - strand, within IAI47_06250 at 1271.586 kb on - strand, within IAI47_06250 at 1271.586 kb on - strand, within IAI47_06250 at 1271.586 kb on - strand, within IAI47_06250 at 1271.586 kb on - strand, within IAI47_06250 at 1271.586 kb on - strand, within IAI47_06250 at 1271.586 kb on - strand, within IAI47_06250 at 1271.586 kb on - strand, within IAI47_06250 at 1271.586 kb on - strand, within IAI47_06250 at 1271.586 kb on - strand, within IAI47_06250 at 1271.632 kb on + strand, within IAI47_06250 at 1271.633 kb on - strand, within IAI47_06250 at 1271.652 kb on + strand, within IAI47_06250 at 1271.661 kb on + strand, within IAI47_06250 at 1271.720 kb on + strand, within IAI47_06250 at 1271.777 kb on + strand at 1271.777 kb on + strand at 1271.797 kb on - strand at 1271.814 kb on + strand at 1271.855 kb on + strand at 1271.855 kb on + strand at 1271.869 kb on + strand at 1271.869 kb on + strand at 1271.869 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,270,183 + IAI47_06240 0.88 +2.0 1,270,183 + IAI47_06240 0.88 +1.2 1,270,184 - IAI47_06240 0.88 +0.2 1,270,190 + IAI47_06240 0.89 +1.5 1,270,190 + IAI47_06240 0.89 -0.5 1,270,191 - IAI47_06240 0.89 -0.6 1,270,196 - IAI47_06240 0.90 -1.8 1,270,206 + +1.3 1,270,206 + -0.8 1,270,234 + +0.1 1,270,292 + -0.3 1,270,292 + -2.3 1,270,292 + -2.5 1,270,345 + +1.4 1,270,364 - +0.4 1,270,409 + IAI47_06245 0.13 -1.2 1,270,651 + IAI47_06245 0.43 -0.5 1,270,652 - IAI47_06245 0.43 -0.6 1,270,660 + IAI47_06245 0.44 -0.3 1,270,661 - IAI47_06245 0.45 -0.4 1,270,712 - IAI47_06245 0.51 +0.2 1,270,712 - IAI47_06245 0.51 +1.7 1,270,712 - IAI47_06245 0.51 -0.7 1,270,858 + IAI47_06245 0.69 -0.7 1,270,858 + IAI47_06245 0.69 -0.3 1,270,859 - IAI47_06245 0.69 +0.4 1,270,934 + IAI47_06245 0.78 +0.6 1,270,934 + IAI47_06245 0.78 -1.0 1,270,935 - IAI47_06245 0.79 +0.9 1,270,947 + IAI47_06245 0.80 -0.7 1,271,030 - +0.4 1,271,047 + +0.3 1,271,104 + -0.1 1,271,104 + -0.3 1,271,104 + -1.4 1,271,109 + -0.1 1,271,122 + -2.6 1,271,123 - -0.8 1,271,123 - +0.6 1,271,123 - +1.2 1,271,165 + +1.1 1,271,166 - -0.6 1,271,208 + IAI47_06250 0.11 -0.9 1,271,208 + IAI47_06250 0.11 -0.8 1,271,208 + IAI47_06250 0.11 -1.2 1,271,209 - IAI47_06250 0.11 -1.0 1,271,214 - IAI47_06250 0.12 +0.5 1,271,214 - IAI47_06250 0.12 -0.2 1,271,223 + IAI47_06250 0.13 -0.7 1,271,223 + IAI47_06250 0.13 -0.6 1,271,223 + IAI47_06250 0.13 -1.8 1,271,224 - IAI47_06250 0.13 -0.0 1,271,228 + IAI47_06250 0.14 -2.4 1,271,229 - IAI47_06250 0.14 +0.0 1,271,333 + IAI47_06250 0.30 -2.9 1,271,333 + IAI47_06250 0.30 +0.3 1,271,334 - IAI47_06250 0.30 -1.0 1,271,354 + IAI47_06250 0.33 +0.2 1,271,354 + IAI47_06250 0.33 -0.8 1,271,354 + IAI47_06250 0.33 +2.5 1,271,354 + IAI47_06250 0.33 -0.6 1,271,354 + IAI47_06250 0.33 +2.0 1,271,354 + IAI47_06250 0.33 +0.7 1,271,354 + IAI47_06250 0.33 -1.6 1,271,354 + IAI47_06250 0.33 -1.2 1,271,355 - IAI47_06250 0.33 +0.8 1,271,355 - IAI47_06250 0.33 -1.7 1,271,355 - IAI47_06250 0.33 -1.4 1,271,355 - IAI47_06250 0.33 -0.2 1,271,355 - IAI47_06250 0.33 -2.5 1,271,355 - IAI47_06250 0.33 -0.8 1,271,355 - IAI47_06250 0.33 +0.5 1,271,355 - IAI47_06250 0.33 -1.7 1,271,368 + IAI47_06250 0.35 -1.4 1,271,403 + IAI47_06250 0.40 -1.5 1,271,403 + IAI47_06250 0.40 +0.2 1,271,403 + IAI47_06250 0.40 +0.2 1,271,403 + IAI47_06250 0.40 -1.2 1,271,403 + IAI47_06250 0.40 +0.6 1,271,496 + IAI47_06250 0.54 -0.1 1,271,522 + IAI47_06250 0.58 +1.7 1,271,523 - IAI47_06250 0.58 +0.2 1,271,585 + IAI47_06250 0.68 +1.5 1,271,585 + IAI47_06250 0.68 -0.4 1,271,585 + IAI47_06250 0.68 +1.5 1,271,585 + IAI47_06250 0.68 +4.0 1,271,585 + IAI47_06250 0.68 -1.1 1,271,585 + IAI47_06250 0.68 -0.3 1,271,585 + IAI47_06250 0.68 +0.3 1,271,585 + IAI47_06250 0.68 -0.1 1,271,586 - IAI47_06250 0.68 +0.7 1,271,586 - IAI47_06250 0.68 -0.3 1,271,586 - IAI47_06250 0.68 -0.9 1,271,586 - IAI47_06250 0.68 -0.1 1,271,586 - IAI47_06250 0.68 -1.1 1,271,586 - IAI47_06250 0.68 +0.1 1,271,586 - IAI47_06250 0.68 -1.0 1,271,586 - IAI47_06250 0.68 -0.9 1,271,586 - IAI47_06250 0.68 -1.8 1,271,586 - IAI47_06250 0.68 +2.2 1,271,586 - IAI47_06250 0.68 -0.7 1,271,586 - IAI47_06250 0.68 -0.4 1,271,586 - IAI47_06250 0.68 +0.5 1,271,632 + IAI47_06250 0.75 -3.1 1,271,633 - IAI47_06250 0.75 -0.1 1,271,652 + IAI47_06250 0.78 -1.4 1,271,661 + IAI47_06250 0.79 -0.1 1,271,720 + IAI47_06250 0.88 -1.4 1,271,777 + -0.7 1,271,777 + +0.7 1,271,797 - -2.0 1,271,814 + -0.8 1,271,855 + -1.0 1,271,855 + -0.1 1,271,869 + +1.2 1,271,869 + +0.2 1,271,869 + +0.3
Or see this region's nucleotide sequence