Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_06145 and IAI47_06150 are separated by 45 nucleotides IAI47_06150 and IAI47_06155 are separated by 4 nucleotides IAI47_06155 and IAI47_06160 are separated by 8 nucleotides
IAI47_06145: IAI47_06145 - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 1,250,291 to 1,251,223
_06145
IAI47_06150: IAI47_06150 - chorismate synthase, at 1,251,269 to 1,252,354
_06150
IAI47_06155: IAI47_06155 - penicillin-insensitive murein endopeptidase, at 1,252,359 to 1,253,183
_06155
IAI47_06160: IAI47_06160 - TSUP family transporter, at 1,253,192 to 1,253,995
_06160
Position (kb)
1251
1252
1253 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1250.401 kb on + strand, within IAI47_06145 at 1250.401 kb on + strand, within IAI47_06145 at 1250.464 kb on + strand, within IAI47_06145 at 1250.476 kb on + strand, within IAI47_06145 at 1250.476 kb on + strand, within IAI47_06145 at 1250.476 kb on + strand, within IAI47_06145 at 1250.564 kb on + strand, within IAI47_06145 at 1250.564 kb on + strand, within IAI47_06145 at 1250.564 kb on + strand, within IAI47_06145 at 1250.575 kb on + strand, within IAI47_06145 at 1250.575 kb on + strand, within IAI47_06145 at 1250.575 kb on + strand, within IAI47_06145 at 1250.575 kb on + strand, within IAI47_06145 at 1250.575 kb on + strand, within IAI47_06145 at 1250.695 kb on + strand, within IAI47_06145 at 1250.695 kb on + strand, within IAI47_06145 at 1250.704 kb on + strand, within IAI47_06145 at 1250.704 kb on + strand, within IAI47_06145 at 1250.704 kb on + strand, within IAI47_06145 at 1250.704 kb on + strand, within IAI47_06145 at 1250.705 kb on + strand, within IAI47_06145 at 1250.779 kb on + strand, within IAI47_06145 at 1250.886 kb on + strand, within IAI47_06145 at 1250.886 kb on + strand, within IAI47_06145 at 1250.891 kb on + strand, within IAI47_06145 at 1250.891 kb on + strand, within IAI47_06145 at 1250.918 kb on + strand, within IAI47_06145 at 1250.918 kb on + strand, within IAI47_06145 at 1250.918 kb on + strand, within IAI47_06145 at 1250.918 kb on + strand, within IAI47_06145 at 1251.041 kb on + strand, within IAI47_06145 at 1251.041 kb on + strand, within IAI47_06145 at 1251.041 kb on + strand, within IAI47_06145 at 1251.107 kb on + strand, within IAI47_06145 at 1251.124 kb on + strand, within IAI47_06145 at 1251.212 kb on + strand at 1251.212 kb on + strand at 1251.214 kb on + strand at 1252.330 kb on + strand at 1252.331 kb on - strand at 1252.467 kb on - strand, within IAI47_06155 at 1252.467 kb on - strand, within IAI47_06155 at 1252.490 kb on + strand, within IAI47_06155 at 1252.490 kb on + strand, within IAI47_06155 at 1252.490 kb on + strand, within IAI47_06155 at 1252.491 kb on - strand, within IAI47_06155 at 1252.491 kb on - strand, within IAI47_06155 at 1252.491 kb on - strand, within IAI47_06155 at 1252.491 kb on - strand, within IAI47_06155 at 1252.491 kb on - strand, within IAI47_06155 at 1252.491 kb on - strand, within IAI47_06155 at 1252.530 kb on + strand, within IAI47_06155 at 1252.530 kb on + strand, within IAI47_06155 at 1252.530 kb on + strand, within IAI47_06155 at 1252.530 kb on + strand, within IAI47_06155 at 1252.531 kb on - strand, within IAI47_06155 at 1252.531 kb on - strand, within IAI47_06155 at 1252.531 kb on - strand, within IAI47_06155 at 1252.531 kb on - strand, within IAI47_06155 at 1252.561 kb on - strand, within IAI47_06155 at 1252.589 kb on + strand, within IAI47_06155 at 1252.590 kb on - strand, within IAI47_06155 at 1252.616 kb on + strand, within IAI47_06155 at 1252.616 kb on + strand, within IAI47_06155 at 1252.616 kb on + strand, within IAI47_06155 at 1252.617 kb on - strand, within IAI47_06155 at 1252.714 kb on + strand, within IAI47_06155 at 1252.714 kb on + strand, within IAI47_06155 at 1252.714 kb on + strand, within IAI47_06155 at 1252.714 kb on + strand, within IAI47_06155 at 1252.714 kb on + strand, within IAI47_06155 at 1252.714 kb on + strand, within IAI47_06155 at 1252.714 kb on + strand, within IAI47_06155 at 1252.715 kb on - strand, within IAI47_06155 at 1252.715 kb on - strand, within IAI47_06155 at 1252.715 kb on - strand, within IAI47_06155 at 1252.715 kb on - strand, within IAI47_06155 at 1252.715 kb on - strand, within IAI47_06155 at 1252.715 kb on - strand, within IAI47_06155 at 1252.715 kb on - strand, within IAI47_06155 at 1252.715 kb on - strand, within IAI47_06155 at 1252.715 kb on - strand, within IAI47_06155 at 1252.718 kb on + strand, within IAI47_06155 at 1252.718 kb on + strand, within IAI47_06155 at 1252.718 kb on + strand, within IAI47_06155 at 1252.718 kb on + strand, within IAI47_06155 at 1252.719 kb on - strand, within IAI47_06155 at 1252.719 kb on - strand, within IAI47_06155 at 1252.719 kb on - strand, within IAI47_06155 at 1252.719 kb on - strand, within IAI47_06155 at 1252.719 kb on - strand, within IAI47_06155 at 1252.719 kb on - strand, within IAI47_06155 at 1252.851 kb on - strand, within IAI47_06155 at 1252.931 kb on + strand, within IAI47_06155 at 1252.952 kb on + strand, within IAI47_06155 at 1252.952 kb on + strand, within IAI47_06155 at 1252.953 kb on - strand, within IAI47_06155 at 1252.953 kb on - strand, within IAI47_06155 at 1252.953 kb on - strand, within IAI47_06155 at 1252.985 kb on + strand, within IAI47_06155 at 1252.985 kb on + strand, within IAI47_06155 at 1252.985 kb on + strand, within IAI47_06155 at 1252.985 kb on + strand, within IAI47_06155 at 1252.985 kb on + strand, within IAI47_06155 at 1252.986 kb on - strand, within IAI47_06155 at 1252.986 kb on - strand, within IAI47_06155 at 1252.986 kb on - strand, within IAI47_06155 at 1252.986 kb on - strand, within IAI47_06155 at 1252.986 kb on - strand, within IAI47_06155 at 1252.986 kb on - strand, within IAI47_06155 at 1253.168 kb on + strand at 1253.176 kb on + strand at 1253.176 kb on + strand at 1253.176 kb on + strand at 1253.177 kb on - strand at 1253.177 kb on - strand at 1253.177 kb on - strand at 1253.181 kb on + strand at 1253.181 kb on + strand at 1253.181 kb on + strand at 1253.261 kb on - strand at 1253.289 kb on + strand, within IAI47_06160 at 1253.289 kb on + strand, within IAI47_06160 at 1253.289 kb on + strand, within IAI47_06160 at 1253.289 kb on + strand, within IAI47_06160 at 1253.289 kb on + strand, within IAI47_06160 at 1253.290 kb on - strand, within IAI47_06160 at 1253.290 kb on - strand, within IAI47_06160 at 1253.290 kb on - strand, within IAI47_06160 at 1253.293 kb on + strand, within IAI47_06160 at 1253.293 kb on + strand, within IAI47_06160 at 1253.293 kb on + strand, within IAI47_06160 at 1253.293 kb on + strand, within IAI47_06160 at 1253.294 kb on - strand, within IAI47_06160 at 1253.294 kb on - strand, within IAI47_06160 at 1253.294 kb on - strand, within IAI47_06160 at 1253.294 kb on - strand, within IAI47_06160 at 1253.294 kb on - strand, within IAI47_06160 at 1253.294 kb on - strand, within IAI47_06160 at 1253.299 kb on - strand, within IAI47_06160 at 1253.299 kb on - strand, within IAI47_06160 at 1253.351 kb on - strand, within IAI47_06160
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,250,401 + IAI47_06145 0.12 +0.3 1,250,401 + IAI47_06145 0.12 +1.3 1,250,464 + IAI47_06145 0.19 -2.0 1,250,476 + IAI47_06145 0.20 -1.5 1,250,476 + IAI47_06145 0.20 -0.2 1,250,476 + IAI47_06145 0.20 -0.1 1,250,564 + IAI47_06145 0.29 -0.6 1,250,564 + IAI47_06145 0.29 +1.2 1,250,564 + IAI47_06145 0.29 +0.4 1,250,575 + IAI47_06145 0.30 -0.9 1,250,575 + IAI47_06145 0.30 +1.1 1,250,575 + IAI47_06145 0.30 -0.6 1,250,575 + IAI47_06145 0.30 -1.6 1,250,575 + IAI47_06145 0.30 -1.1 1,250,695 + IAI47_06145 0.43 -0.6 1,250,695 + IAI47_06145 0.43 +1.1 1,250,704 + IAI47_06145 0.44 -1.5 1,250,704 + IAI47_06145 0.44 -0.8 1,250,704 + IAI47_06145 0.44 -1.6 1,250,704 + IAI47_06145 0.44 +1.0 1,250,705 + IAI47_06145 0.44 -0.3 1,250,779 + IAI47_06145 0.52 -0.1 1,250,886 + IAI47_06145 0.64 +0.9 1,250,886 + IAI47_06145 0.64 +1.5 1,250,891 + IAI47_06145 0.64 -0.1 1,250,891 + IAI47_06145 0.64 -2.5 1,250,918 + IAI47_06145 0.67 -0.7 1,250,918 + IAI47_06145 0.67 -1.0 1,250,918 + IAI47_06145 0.67 -1.0 1,250,918 + IAI47_06145 0.67 -0.2 1,251,041 + IAI47_06145 0.80 -1.1 1,251,041 + IAI47_06145 0.80 -0.3 1,251,041 + IAI47_06145 0.80 -1.2 1,251,107 + IAI47_06145 0.87 -0.1 1,251,124 + IAI47_06145 0.89 +0.7 1,251,212 + -1.1 1,251,212 + -0.6 1,251,214 + -2.0 1,252,330 + -1.4 1,252,331 - +0.7 1,252,467 - IAI47_06155 0.13 +0.2 1,252,467 - IAI47_06155 0.13 +0.2 1,252,490 + IAI47_06155 0.16 +1.1 1,252,490 + IAI47_06155 0.16 +0.7 1,252,490 + IAI47_06155 0.16 -0.5 1,252,491 - IAI47_06155 0.16 -0.6 1,252,491 - IAI47_06155 0.16 +2.6 1,252,491 - IAI47_06155 0.16 -1.7 1,252,491 - IAI47_06155 0.16 +0.2 1,252,491 - IAI47_06155 0.16 +1.0 1,252,491 - IAI47_06155 0.16 -0.4 1,252,530 + IAI47_06155 0.21 -0.2 1,252,530 + IAI47_06155 0.21 +0.5 1,252,530 + IAI47_06155 0.21 -1.2 1,252,530 + IAI47_06155 0.21 -0.1 1,252,531 - IAI47_06155 0.21 +0.4 1,252,531 - IAI47_06155 0.21 -1.3 1,252,531 - IAI47_06155 0.21 -0.3 1,252,531 - IAI47_06155 0.21 +0.7 1,252,561 - IAI47_06155 0.24 +0.7 1,252,589 + IAI47_06155 0.28 +1.2 1,252,590 - IAI47_06155 0.28 -1.8 1,252,616 + IAI47_06155 0.31 +0.7 1,252,616 + IAI47_06155 0.31 -0.5 1,252,616 + IAI47_06155 0.31 -0.4 1,252,617 - IAI47_06155 0.31 -1.4 1,252,714 + IAI47_06155 0.43 -0.6 1,252,714 + IAI47_06155 0.43 +1.0 1,252,714 + IAI47_06155 0.43 -0.8 1,252,714 + IAI47_06155 0.43 -1.5 1,252,714 + IAI47_06155 0.43 -1.0 1,252,714 + IAI47_06155 0.43 +0.5 1,252,714 + IAI47_06155 0.43 +0.4 1,252,715 - IAI47_06155 0.43 -0.3 1,252,715 - IAI47_06155 0.43 -0.1 1,252,715 - IAI47_06155 0.43 -0.3 1,252,715 - IAI47_06155 0.43 -0.3 1,252,715 - IAI47_06155 0.43 -0.1 1,252,715 - IAI47_06155 0.43 +0.0 1,252,715 - IAI47_06155 0.43 -0.8 1,252,715 - IAI47_06155 0.43 -0.0 1,252,715 - IAI47_06155 0.43 +1.1 1,252,718 + IAI47_06155 0.44 +0.2 1,252,718 + IAI47_06155 0.44 +1.6 1,252,718 + IAI47_06155 0.44 +0.8 1,252,718 + IAI47_06155 0.44 -1.3 1,252,719 - IAI47_06155 0.44 +0.2 1,252,719 - IAI47_06155 0.44 +0.2 1,252,719 - IAI47_06155 0.44 -0.8 1,252,719 - IAI47_06155 0.44 +0.7 1,252,719 - IAI47_06155 0.44 -0.5 1,252,719 - IAI47_06155 0.44 -0.3 1,252,851 - IAI47_06155 0.60 +0.2 1,252,931 + IAI47_06155 0.69 -1.0 1,252,952 + IAI47_06155 0.72 +1.0 1,252,952 + IAI47_06155 0.72 +1.7 1,252,953 - IAI47_06155 0.72 -0.6 1,252,953 - IAI47_06155 0.72 +1.7 1,252,953 - IAI47_06155 0.72 +0.2 1,252,985 + IAI47_06155 0.76 -1.7 1,252,985 + IAI47_06155 0.76 +1.7 1,252,985 + IAI47_06155 0.76 -0.6 1,252,985 + IAI47_06155 0.76 -1.7 1,252,985 + IAI47_06155 0.76 -0.2 1,252,986 - IAI47_06155 0.76 +0.9 1,252,986 - IAI47_06155 0.76 +3.2 1,252,986 - IAI47_06155 0.76 +0.6 1,252,986 - IAI47_06155 0.76 +2.6 1,252,986 - IAI47_06155 0.76 +1.0 1,252,986 - IAI47_06155 0.76 -0.2 1,253,168 + -2.2 1,253,176 + +1.2 1,253,176 + -1.3 1,253,176 + -0.8 1,253,177 - +0.6 1,253,177 - -0.6 1,253,177 - +1.0 1,253,181 + -1.0 1,253,181 + +0.1 1,253,181 + +1.7 1,253,261 - +0.2 1,253,289 + IAI47_06160 0.12 -0.4 1,253,289 + IAI47_06160 0.12 +3.7 1,253,289 + IAI47_06160 0.12 +0.6 1,253,289 + IAI47_06160 0.12 -0.3 1,253,289 + IAI47_06160 0.12 -0.5 1,253,290 - IAI47_06160 0.12 +1.1 1,253,290 - IAI47_06160 0.12 +1.3 1,253,290 - IAI47_06160 0.12 -1.9 1,253,293 + IAI47_06160 0.13 +1.0 1,253,293 + IAI47_06160 0.13 -2.1 1,253,293 + IAI47_06160 0.13 -2.0 1,253,293 + IAI47_06160 0.13 +2.0 1,253,294 - IAI47_06160 0.13 +1.1 1,253,294 - IAI47_06160 0.13 +1.3 1,253,294 - IAI47_06160 0.13 -1.5 1,253,294 - IAI47_06160 0.13 +0.2 1,253,294 - IAI47_06160 0.13 -0.7 1,253,294 - IAI47_06160 0.13 -1.2 1,253,299 - IAI47_06160 0.13 -0.8 1,253,299 - IAI47_06160 0.13 +0.5 1,253,351 - IAI47_06160 0.20 -1.0
Or see this region's nucleotide sequence