Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_03090 and IAI47_03095 are separated by 272 nucleotides IAI47_03095 and IAI47_03100 are separated by 33 nucleotides
IAI47_03090: IAI47_03090 - SH3 domain-containing protein, at 638,255 to 638,875
_03090
IAI47_03095: IAI47_03095 - inorganic triphosphatase, at 639,148 to 640,458
_03095
IAI47_03100: IAI47_03100 - bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase, at 640,492 to 643,344
_03100
Position (kb) 639
640
641 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 638.173 kb on + strand at 638.173 kb on + strand at 638.174 kb on - strand at 638.174 kb on - strand at 638.174 kb on - strand at 638.296 kb on + strand at 638.296 kb on + strand at 638.296 kb on + strand at 638.297 kb on - strand at 638.297 kb on - strand at 638.297 kb on - strand at 638.297 kb on - strand at 638.297 kb on - strand at 638.297 kb on - strand at 638.297 kb on - strand at 638.306 kb on + strand at 638.307 kb on - strand at 638.307 kb on - strand at 638.349 kb on + strand, within IAI47_03090 at 638.349 kb on + strand, within IAI47_03090 at 638.349 kb on + strand, within IAI47_03090 at 638.349 kb on + strand, within IAI47_03090 at 638.349 kb on + strand, within IAI47_03090 at 638.349 kb on + strand, within IAI47_03090 at 638.349 kb on + strand, within IAI47_03090 at 638.349 kb on + strand, within IAI47_03090 at 638.349 kb on + strand, within IAI47_03090 at 638.349 kb on + strand, within IAI47_03090 at 638.349 kb on + strand, within IAI47_03090 at 638.349 kb on + strand, within IAI47_03090 at 638.349 kb on + strand, within IAI47_03090 at 638.350 kb on - strand, within IAI47_03090 at 638.350 kb on - strand, within IAI47_03090 at 638.350 kb on - strand, within IAI47_03090 at 638.350 kb on - strand, within IAI47_03090 at 638.350 kb on - strand, within IAI47_03090 at 638.350 kb on - strand, within IAI47_03090 at 638.350 kb on - strand, within IAI47_03090 at 638.350 kb on - strand, within IAI47_03090 at 638.350 kb on - strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.353 kb on + strand, within IAI47_03090 at 638.354 kb on - strand, within IAI47_03090 at 638.354 kb on - strand, within IAI47_03090 at 638.354 kb on - strand, within IAI47_03090 at 638.354 kb on - strand, within IAI47_03090 at 638.354 kb on - strand, within IAI47_03090 at 638.354 kb on - strand, within IAI47_03090 at 638.354 kb on - strand, within IAI47_03090 at 638.354 kb on - strand, within IAI47_03090 at 638.354 kb on - strand, within IAI47_03090 at 638.365 kb on + strand, within IAI47_03090 at 638.365 kb on + strand, within IAI47_03090 at 638.365 kb on + strand, within IAI47_03090 at 638.365 kb on + strand, within IAI47_03090 at 638.366 kb on - strand, within IAI47_03090 at 638.366 kb on - strand, within IAI47_03090 at 638.366 kb on - strand, within IAI47_03090 at 638.366 kb on - strand, within IAI47_03090 at 638.366 kb on - strand, within IAI47_03090 at 638.366 kb on - strand, within IAI47_03090 at 638.366 kb on - strand, within IAI47_03090 at 638.473 kb on + strand, within IAI47_03090 at 638.474 kb on - strand, within IAI47_03090 at 638.474 kb on - strand, within IAI47_03090 at 638.542 kb on + strand, within IAI47_03090 at 638.542 kb on + strand, within IAI47_03090 at 638.543 kb on - strand, within IAI47_03090 at 638.545 kb on + strand, within IAI47_03090 at 638.545 kb on + strand, within IAI47_03090 at 638.546 kb on - strand, within IAI47_03090 at 638.546 kb on - strand, within IAI47_03090 at 638.676 kb on + strand, within IAI47_03090 at 638.676 kb on + strand, within IAI47_03090 at 638.676 kb on + strand, within IAI47_03090 at 638.676 kb on + strand, within IAI47_03090 at 638.677 kb on - strand, within IAI47_03090 at 638.683 kb on + strand, within IAI47_03090 at 638.683 kb on + strand, within IAI47_03090 at 638.684 kb on - strand, within IAI47_03090 at 638.702 kb on + strand, within IAI47_03090 at 638.702 kb on + strand, within IAI47_03090 at 638.703 kb on - strand, within IAI47_03090 at 638.703 kb on - strand, within IAI47_03090 at 638.703 kb on - strand, within IAI47_03090 at 638.731 kb on + strand, within IAI47_03090 at 638.731 kb on + strand, within IAI47_03090 at 638.764 kb on + strand, within IAI47_03090 at 638.765 kb on - strand, within IAI47_03090 at 638.796 kb on + strand, within IAI47_03090 at 638.796 kb on + strand, within IAI47_03090 at 638.796 kb on + strand, within IAI47_03090 at 638.796 kb on + strand, within IAI47_03090 at 638.796 kb on + strand, within IAI47_03090 at 638.797 kb on - strand, within IAI47_03090 at 638.797 kb on - strand, within IAI47_03090 at 638.797 kb on - strand, within IAI47_03090 at 638.833 kb on + strand at 638.833 kb on + strand at 638.834 kb on - strand at 638.893 kb on - strand at 638.902 kb on + strand at 638.937 kb on + strand at 638.937 kb on + strand at 638.937 kb on + strand at 638.938 kb on - strand at 638.938 kb on - strand at 638.954 kb on + strand at 638.954 kb on + strand at 638.954 kb on + strand at 638.954 kb on + strand at 638.954 kb on + strand at 638.954 kb on + strand at 638.954 kb on + strand at 638.954 kb on + strand at 638.955 kb on - strand at 638.955 kb on - strand at 638.955 kb on - strand at 638.955 kb on - strand at 638.955 kb on - strand at 638.955 kb on - strand at 638.955 kb on - strand at 638.955 kb on - strand at 639.029 kb on + strand at 639.030 kb on - strand at 639.074 kb on + strand at 639.075 kb on - strand at 639.098 kb on + strand at 639.098 kb on + strand at 639.098 kb on + strand at 639.099 kb on - strand at 639.099 kb on - strand at 639.099 kb on - strand at 639.099 kb on - strand at 639.117 kb on + strand at 639.117 kb on + strand at 639.118 kb on - strand at 639.118 kb on - strand at 639.118 kb on - strand at 639.140 kb on - strand at 639.167 kb on + strand at 639.167 kb on + strand at 639.265 kb on + strand at 639.266 kb on - strand at 639.266 kb on - strand at 639.266 kb on - strand at 639.266 kb on - strand at 639.315 kb on + strand, within IAI47_03095 at 639.315 kb on + strand, within IAI47_03095 at 639.315 kb on + strand, within IAI47_03095 at 639.337 kb on + strand, within IAI47_03095 at 639.338 kb on - strand, within IAI47_03095 at 639.398 kb on - strand, within IAI47_03095 at 639.398 kb on - strand, within IAI47_03095 at 639.398 kb on - strand, within IAI47_03095 at 639.404 kb on + strand, within IAI47_03095 at 639.404 kb on + strand, within IAI47_03095 at 639.404 kb on + strand, within IAI47_03095 at 639.404 kb on + strand, within IAI47_03095 at 639.404 kb on + strand, within IAI47_03095 at 639.405 kb on - strand, within IAI47_03095 at 639.405 kb on - strand, within IAI47_03095 at 639.405 kb on - strand, within IAI47_03095 at 639.405 kb on - strand, within IAI47_03095 at 639.405 kb on - strand, within IAI47_03095 at 639.405 kb on - strand, within IAI47_03095 at 639.422 kb on + strand, within IAI47_03095 at 639.423 kb on - strand, within IAI47_03095 at 639.495 kb on - strand, within IAI47_03095 at 639.495 kb on - strand, within IAI47_03095 at 639.495 kb on - strand, within IAI47_03095 at 639.495 kb on - strand, within IAI47_03095 at 639.629 kb on + strand, within IAI47_03095 at 639.629 kb on + strand, within IAI47_03095 at 639.630 kb on - strand, within IAI47_03095 at 639.702 kb on + strand, within IAI47_03095 at 639.702 kb on + strand, within IAI47_03095 at 639.703 kb on - strand, within IAI47_03095 at 639.734 kb on - strand, within IAI47_03095 at 639.734 kb on - strand, within IAI47_03095 at 639.750 kb on + strand, within IAI47_03095 at 639.751 kb on - strand, within IAI47_03095 at 639.751 kb on - strand, within IAI47_03095 at 639.751 kb on - strand, within IAI47_03095 at 639.819 kb on + strand, within IAI47_03095 at 639.819 kb on + strand, within IAI47_03095 at 639.819 kb on + strand, within IAI47_03095 at 639.820 kb on - strand, within IAI47_03095 at 639.859 kb on + strand, within IAI47_03095 at 639.859 kb on + strand, within IAI47_03095 at 639.859 kb on + strand, within IAI47_03095 at 639.859 kb on + strand, within IAI47_03095 at 639.860 kb on - strand, within IAI47_03095 at 639.860 kb on - strand, within IAI47_03095 at 639.860 kb on - strand, within IAI47_03095 at 640.055 kb on + strand, within IAI47_03095 at 640.055 kb on + strand, within IAI47_03095 at 640.055 kb on + strand, within IAI47_03095 at 640.055 kb on + strand, within IAI47_03095 at 640.055 kb on + strand, within IAI47_03095 at 640.056 kb on - strand, within IAI47_03095 at 640.056 kb on - strand, within IAI47_03095 at 640.056 kb on - strand, within IAI47_03095 at 640.164 kb on + strand, within IAI47_03095 at 640.179 kb on + strand, within IAI47_03095 at 640.180 kb on - strand, within IAI47_03095 at 640.246 kb on + strand, within IAI47_03095 at 640.246 kb on + strand, within IAI47_03095 at 640.247 kb on - strand, within IAI47_03095 at 640.247 kb on - strand, within IAI47_03095 at 640.254 kb on + strand, within IAI47_03095 at 640.255 kb on - strand, within IAI47_03095 at 640.309 kb on + strand, within IAI47_03095 at 640.309 kb on + strand, within IAI47_03095 at 640.309 kb on + strand, within IAI47_03095 at 640.309 kb on + strand, within IAI47_03095 at 640.310 kb on - strand, within IAI47_03095 at 640.310 kb on - strand, within IAI47_03095 at 640.310 kb on - strand, within IAI47_03095 at 640.491 kb on + strand at 640.491 kb on + strand at 640.491 kb on + strand at 640.491 kb on + strand at 640.491 kb on + strand at 640.491 kb on + strand at 640.491 kb on + strand at 640.491 kb on + strand at 640.491 kb on + strand at 640.491 kb on + strand at 640.491 kb on + strand at 640.491 kb on + strand at 640.491 kb on + strand at 640.491 kb on + strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.492 kb on - strand at 640.693 kb on + strand at 640.693 kb on + strand at 640.693 kb on + strand at 640.693 kb on + strand at 640.693 kb on + strand at 640.693 kb on + strand at 640.694 kb on - strand at 640.694 kb on - strand at 640.776 kb on + strand at 640.776 kb on + strand at 640.776 kb on + strand at 640.777 kb on - strand at 640.777 kb on - strand at 640.777 kb on - strand at 640.777 kb on - strand at 640.785 kb on - strand, within IAI47_03100 at 640.785 kb on - strand, within IAI47_03100 at 640.853 kb on + strand, within IAI47_03100 at 640.854 kb on - strand, within IAI47_03100 at 640.891 kb on + strand, within IAI47_03100 at 640.891 kb on + strand, within IAI47_03100 at 640.891 kb on + strand, within IAI47_03100 at 640.891 kb on + strand, within IAI47_03100 at 640.917 kb on + strand, within IAI47_03100 at 640.927 kb on - strand, within IAI47_03100 at 641.010 kb on - strand, within IAI47_03100 at 641.221 kb on + strand, within IAI47_03100 at 641.221 kb on + strand, within IAI47_03100 at 641.221 kb on + strand, within IAI47_03100 at 641.221 kb on + strand, within IAI47_03100 at 641.221 kb on + strand, within IAI47_03100 at 641.222 kb on - strand, within IAI47_03100 at 641.222 kb on - strand, within IAI47_03100 at 641.222 kb on - strand, within IAI47_03100 at 641.222 kb on - strand, within IAI47_03100 at 641.224 kb on + strand, within IAI47_03100 at 641.225 kb on - strand, within IAI47_03100 at 641.225 kb on - strand, within IAI47_03100 at 641.225 kb on - strand, within IAI47_03100 at 641.225 kb on - strand, within IAI47_03100 at 641.254 kb on + strand, within IAI47_03100 at 641.254 kb on + strand, within IAI47_03100 at 641.290 kb on + strand, within IAI47_03100 at 641.290 kb on + strand, within IAI47_03100 at 641.290 kb on + strand, within IAI47_03100 at 641.290 kb on + strand, within IAI47_03100 at 641.290 kb on + strand, within IAI47_03100 at 641.291 kb on - strand, within IAI47_03100 at 641.291 kb on - strand, within IAI47_03100 at 641.291 kb on - strand, within IAI47_03100 at 641.347 kb on + strand, within IAI47_03100 at 641.348 kb on - strand, within IAI47_03100 at 641.379 kb on + strand, within IAI47_03100 at 641.380 kb on - strand, within IAI47_03100 at 641.433 kb on + strand, within IAI47_03100 at 641.434 kb on - strand, within IAI47_03100
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 638,173 + -0.1 638,173 + -0.4 638,174 - -2.0 638,174 - +0.4 638,174 - +0.1 638,296 + -0.6 638,296 + +1.0 638,296 + -1.5 638,297 - +1.3 638,297 - -2.6 638,297 - -0.3 638,297 - +1.2 638,297 - -0.1 638,297 - +0.1 638,297 - +1.4 638,306 + -0.3 638,307 - +1.9 638,307 - -1.0 638,349 + IAI47_03090 0.15 -1.5 638,349 + IAI47_03090 0.15 +0.8 638,349 + IAI47_03090 0.15 -1.0 638,349 + IAI47_03090 0.15 +0.6 638,349 + IAI47_03090 0.15 +2.0 638,349 + IAI47_03090 0.15 -0.6 638,349 + IAI47_03090 0.15 +0.4 638,349 + IAI47_03090 0.15 -1.5 638,349 + IAI47_03090 0.15 +0.5 638,349 + IAI47_03090 0.15 +0.0 638,349 + IAI47_03090 0.15 -0.3 638,349 + IAI47_03090 0.15 -1.2 638,349 + IAI47_03090 0.15 +0.4 638,350 - IAI47_03090 0.15 -0.2 638,350 - IAI47_03090 0.15 -1.1 638,350 - IAI47_03090 0.15 -0.3 638,350 - IAI47_03090 0.15 +1.2 638,350 - IAI47_03090 0.15 +1.1 638,350 - IAI47_03090 0.15 -2.3 638,350 - IAI47_03090 0.15 +0.6 638,350 - IAI47_03090 0.15 -2.1 638,350 - IAI47_03090 0.15 -0.2 638,353 + IAI47_03090 0.16 -0.9 638,353 + IAI47_03090 0.16 +0.4 638,353 + IAI47_03090 0.16 -1.9 638,353 + IAI47_03090 0.16 -0.9 638,353 + IAI47_03090 0.16 +0.2 638,353 + IAI47_03090 0.16 -1.3 638,353 + IAI47_03090 0.16 +0.9 638,353 + IAI47_03090 0.16 +0.7 638,353 + IAI47_03090 0.16 -1.2 638,353 + IAI47_03090 0.16 -1.0 638,353 + IAI47_03090 0.16 -0.0 638,353 + IAI47_03090 0.16 -0.1 638,353 + IAI47_03090 0.16 +1.9 638,353 + IAI47_03090 0.16 +0.8 638,353 + IAI47_03090 0.16 -0.2 638,353 + IAI47_03090 0.16 +1.4 638,353 + IAI47_03090 0.16 +0.6 638,353 + IAI47_03090 0.16 -0.8 638,353 + IAI47_03090 0.16 -0.7 638,353 + IAI47_03090 0.16 +0.4 638,354 - IAI47_03090 0.16 +0.7 638,354 - IAI47_03090 0.16 -0.7 638,354 - IAI47_03090 0.16 -0.1 638,354 - IAI47_03090 0.16 -1.3 638,354 - IAI47_03090 0.16 -0.5 638,354 - IAI47_03090 0.16 +1.1 638,354 - IAI47_03090 0.16 +0.6 638,354 - IAI47_03090 0.16 +0.6 638,354 - IAI47_03090 0.16 -1.9 638,365 + IAI47_03090 0.18 -1.2 638,365 + IAI47_03090 0.18 -0.9 638,365 + IAI47_03090 0.18 -1.1 638,365 + IAI47_03090 0.18 +0.0 638,366 - IAI47_03090 0.18 -0.1 638,366 - IAI47_03090 0.18 -0.6 638,366 - IAI47_03090 0.18 +0.8 638,366 - IAI47_03090 0.18 +0.3 638,366 - IAI47_03090 0.18 -0.8 638,366 - IAI47_03090 0.18 -0.2 638,366 - IAI47_03090 0.18 +1.6 638,473 + IAI47_03090 0.35 +0.5 638,474 - IAI47_03090 0.35 -0.8 638,474 - IAI47_03090 0.35 +0.5 638,542 + IAI47_03090 0.46 -0.6 638,542 + IAI47_03090 0.46 -1.9 638,543 - IAI47_03090 0.46 -1.1 638,545 + IAI47_03090 0.47 -1.1 638,545 + IAI47_03090 0.47 -1.8 638,546 - IAI47_03090 0.47 +2.4 638,546 - IAI47_03090 0.47 -0.3 638,676 + IAI47_03090 0.68 -0.5 638,676 + IAI47_03090 0.68 +1.1 638,676 + IAI47_03090 0.68 +0.8 638,676 + IAI47_03090 0.68 -0.8 638,677 - IAI47_03090 0.68 +0.1 638,683 + IAI47_03090 0.69 -0.1 638,683 + IAI47_03090 0.69 -0.9 638,684 - IAI47_03090 0.69 -0.6 638,702 + IAI47_03090 0.72 +1.1 638,702 + IAI47_03090 0.72 -0.9 638,703 - IAI47_03090 0.72 -1.6 638,703 - IAI47_03090 0.72 +0.4 638,703 - IAI47_03090 0.72 +1.5 638,731 + IAI47_03090 0.77 +0.9 638,731 + IAI47_03090 0.77 -2.8 638,764 + IAI47_03090 0.82 +1.5 638,765 - IAI47_03090 0.82 -0.2 638,796 + IAI47_03090 0.87 +1.7 638,796 + IAI47_03090 0.87 -2.1 638,796 + IAI47_03090 0.87 +0.8 638,796 + IAI47_03090 0.87 +1.3 638,796 + IAI47_03090 0.87 +0.1 638,797 - IAI47_03090 0.87 -2.1 638,797 - IAI47_03090 0.87 -1.4 638,797 - IAI47_03090 0.87 -1.8 638,833 + -0.6 638,833 + -0.6 638,834 - -0.6 638,893 - +1.2 638,902 + -0.3 638,937 + -1.0 638,937 + -0.0 638,937 + -0.1 638,938 - +0.5 638,938 - -1.3 638,954 + -1.0 638,954 + -1.5 638,954 + -0.2 638,954 + +1.1 638,954 + -0.6 638,954 + +0.3 638,954 + +1.2 638,954 + +1.8 638,955 - +0.8 638,955 - +0.1 638,955 - +0.7 638,955 - +0.3 638,955 - +1.0 638,955 - -0.3 638,955 - +0.4 638,955 - -0.5 639,029 + -0.6 639,030 - +0.7 639,074 + +2.2 639,075 - -1.3 639,098 + +0.5 639,098 + -0.2 639,098 + -2.0 639,099 - -0.9 639,099 - +0.9 639,099 - -1.0 639,099 - +2.4 639,117 + +1.5 639,117 + -1.4 639,118 - -0.5 639,118 - -0.4 639,118 - -0.7 639,140 - +2.1 639,167 + +0.8 639,167 + -2.3 639,265 + -1.0 639,266 - -0.1 639,266 - +0.3 639,266 - +0.4 639,266 - -0.7 639,315 + IAI47_03095 0.13 -1.2 639,315 + IAI47_03095 0.13 +0.8 639,315 + IAI47_03095 0.13 +1.5 639,337 + IAI47_03095 0.14 -0.6 639,338 - IAI47_03095 0.14 -0.2 639,398 - IAI47_03095 0.19 -1.1 639,398 - IAI47_03095 0.19 -0.4 639,398 - IAI47_03095 0.19 +1.6 639,404 + IAI47_03095 0.20 -1.5 639,404 + IAI47_03095 0.20 +0.4 639,404 + IAI47_03095 0.20 +0.9 639,404 + IAI47_03095 0.20 -0.7 639,404 + IAI47_03095 0.20 +0.5 639,405 - IAI47_03095 0.20 -1.0 639,405 - IAI47_03095 0.20 -0.8 639,405 - IAI47_03095 0.20 +0.8 639,405 - IAI47_03095 0.20 -1.8 639,405 - IAI47_03095 0.20 -1.7 639,405 - IAI47_03095 0.20 +0.2 639,422 + IAI47_03095 0.21 +0.2 639,423 - IAI47_03095 0.21 +2.5 639,495 - IAI47_03095 0.26 -0.5 639,495 - IAI47_03095 0.26 -0.4 639,495 - IAI47_03095 0.26 -0.2 639,495 - IAI47_03095 0.26 -1.6 639,629 + IAI47_03095 0.37 +1.9 639,629 + IAI47_03095 0.37 +1.3 639,630 - 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IAI47_03100 0.28 +0.7 641,291 - IAI47_03100 0.28 -0.9 641,291 - IAI47_03100 0.28 -0.2 641,347 + IAI47_03100 0.30 +1.0 641,348 - IAI47_03100 0.30 -1.6 641,379 + IAI47_03100 0.31 +0.6 641,380 - IAI47_03100 0.31 +0.0 641,433 + IAI47_03100 0.33 -0.7 641,434 - IAI47_03100 0.33 -1.8
Or see this region's nucleotide sequence