Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01365 and IAI47_01370 are separated by 242 nucleotides IAI47_01370 and IAI47_01375 are separated by 7 nucleotides
IAI47_01365: IAI47_01365 - inorganic phosphate transporter PitA, at 294,272 to 295,774
_01365
IAI47_01370: IAI47_01370 - NAD(P)/FAD-dependent oxidoreductase, at 296,017 to 297,201
_01370
IAI47_01375: IAI47_01375 - aldo/keto reductase, at 297,209 to 298,195
_01375
Position (kb) 296
297
298 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 295.108 kb on - strand, within IAI47_01365 at 295.201 kb on + strand, within IAI47_01365 at 295.541 kb on - strand at 295.541 kb on - strand at 295.645 kb on - strand at 295.739 kb on - strand at 295.752 kb on - strand at 295.769 kb on + strand at 295.774 kb on + strand at 295.857 kb on + strand at 295.857 kb on + strand at 295.857 kb on + strand at 295.857 kb on + strand at 295.907 kb on + strand at 295.907 kb on + strand at 295.912 kb on - strand at 295.912 kb on - strand at 295.912 kb on - strand at 295.912 kb on - strand at 295.914 kb on + strand at 295.922 kb on + strand at 295.927 kb on + strand at 295.928 kb on - strand at 295.981 kb on - strand at 296.001 kb on + strand at 296.001 kb on + strand at 296.002 kb on - strand at 296.034 kb on + strand at 296.106 kb on - strand at 296.106 kb on - strand at 296.182 kb on + strand, within IAI47_01370 at 296.183 kb on - strand, within IAI47_01370 at 296.200 kb on + strand, within IAI47_01370 at 296.201 kb on - strand, within IAI47_01370 at 296.201 kb on - strand, within IAI47_01370 at 296.201 kb on - strand, within IAI47_01370 at 296.201 kb on - strand, within IAI47_01370 at 296.227 kb on - strand, within IAI47_01370 at 296.227 kb on - strand, within IAI47_01370 at 296.245 kb on + strand, within IAI47_01370 at 296.245 kb on + strand, within IAI47_01370 at 296.246 kb on - strand, within IAI47_01370 at 296.246 kb on - strand, within IAI47_01370 at 296.247 kb on + strand, within IAI47_01370 at 296.247 kb on + strand, within IAI47_01370 at 296.247 kb on + strand, within IAI47_01370 at 296.247 kb on + strand, within IAI47_01370 at 296.248 kb on - strand, within IAI47_01370 at 296.263 kb on - strand, within IAI47_01370 at 296.263 kb on - strand, within IAI47_01370 at 296.263 kb on - strand, within IAI47_01370 at 296.268 kb on + strand, within IAI47_01370 at 296.274 kb on + strand, within IAI47_01370 at 296.437 kb on + strand, within IAI47_01370 at 296.437 kb on + strand, within IAI47_01370 at 296.437 kb on + strand, within IAI47_01370 at 296.438 kb on - strand, within IAI47_01370 at 296.438 kb on - strand, within IAI47_01370 at 296.569 kb on + strand, within IAI47_01370 at 296.569 kb on + strand, within IAI47_01370 at 296.570 kb on - strand, within IAI47_01370 at 296.598 kb on + strand, within IAI47_01370 at 296.598 kb on + strand, within IAI47_01370 at 296.598 kb on + strand, within IAI47_01370 at 296.599 kb on - strand, within IAI47_01370 at 296.607 kb on + strand, within IAI47_01370 at 296.607 kb on + strand, within IAI47_01370 at 296.607 kb on + strand, within IAI47_01370 at 296.607 kb on + strand, within IAI47_01370 at 296.607 kb on + strand, within IAI47_01370 at 296.607 kb on + strand, within IAI47_01370 at 296.608 kb on - strand, within IAI47_01370 at 296.608 kb on - strand, within IAI47_01370 at 296.608 kb on - strand, within IAI47_01370 at 296.627 kb on + strand, within IAI47_01370 at 296.627 kb on + strand, within IAI47_01370 at 296.627 kb on + strand, within IAI47_01370 at 296.627 kb on + strand, within IAI47_01370 at 296.627 kb on + strand, within IAI47_01370 at 296.628 kb on - strand, within IAI47_01370 at 296.628 kb on - strand, within IAI47_01370 at 296.628 kb on - strand, within IAI47_01370 at 296.628 kb on - strand, within IAI47_01370 at 296.679 kb on + strand, within IAI47_01370 at 296.688 kb on + strand, within IAI47_01370 at 296.688 kb on + strand, within IAI47_01370 at 296.735 kb on + strand, within IAI47_01370 at 296.752 kb on + strand, within IAI47_01370 at 296.753 kb on - strand, within IAI47_01370 at 296.898 kb on - strand, within IAI47_01370 at 296.898 kb on - strand, within IAI47_01370 at 296.898 kb on - strand, within IAI47_01370 at 296.898 kb on - strand, within IAI47_01370 at 296.898 kb on - strand, within IAI47_01370 at 297.016 kb on + strand, within IAI47_01370 at 297.047 kb on + strand, within IAI47_01370 at 297.048 kb on - strand, within IAI47_01370 at 297.103 kb on + strand at 297.103 kb on + strand at 297.103 kb on + strand at 297.104 kb on - strand at 297.104 kb on - strand at 297.104 kb on - strand at 297.104 kb on - strand at 297.108 kb on + strand at 297.109 kb on - strand at 297.109 kb on - strand at 297.109 kb on - strand at 297.109 kb on - strand at 297.109 kb on - strand at 297.109 kb on - strand at 297.148 kb on + strand at 297.148 kb on + strand at 297.148 kb on + strand at 297.148 kb on + strand at 297.148 kb on + strand at 297.149 kb on - strand at 297.149 kb on - strand at 297.149 kb on - strand at 297.150 kb on + strand at 297.150 kb on + strand at 297.150 kb on + strand at 297.150 kb on + strand at 297.150 kb on + strand at 297.150 kb on + strand at 297.150 kb on + strand at 297.150 kb on + strand at 297.150 kb on + strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.199 kb on + strand at 297.199 kb on + strand at 297.200 kb on - strand at 297.249 kb on + strand at 297.249 kb on + strand at 297.250 kb on - strand at 297.250 kb on - strand at 297.274 kb on + strand at 297.274 kb on + strand at 297.275 kb on - strand at 297.462 kb on + strand, within IAI47_01375 at 297.462 kb on + strand, within IAI47_01375 at 297.462 kb on + strand, within IAI47_01375 at 297.462 kb on + strand, within IAI47_01375 at 297.462 kb on + strand, within IAI47_01375 at 297.463 kb on - strand, within IAI47_01375 at 297.463 kb on - strand, within IAI47_01375 at 297.463 kb on - strand, within IAI47_01375 at 297.463 kb on - strand, within IAI47_01375 at 297.463 kb on - strand, within IAI47_01375 at 297.463 kb on - strand, within IAI47_01375 at 297.509 kb on - strand, within IAI47_01375 at 297.509 kb on - strand, within IAI47_01375 at 297.669 kb on + strand, within IAI47_01375 at 297.669 kb on + strand, within IAI47_01375 at 297.748 kb on - strand, within IAI47_01375 at 297.748 kb on - strand, within IAI47_01375 at 297.807 kb on + strand, within IAI47_01375 at 297.807 kb on + strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.810 kb on + strand, within IAI47_01375 at 297.810 kb on + strand, within IAI47_01375 at 297.810 kb on + strand, within IAI47_01375 at 297.810 kb on + strand, within IAI47_01375 at 297.811 kb on - strand, within IAI47_01375 at 297.811 kb on - strand, within IAI47_01375 at 297.811 kb on - strand, within IAI47_01375 at 297.811 kb on - strand, within IAI47_01375 at 297.811 kb on - strand, within IAI47_01375 at 297.811 kb on - strand, within IAI47_01375 at 297.887 kb on - strand, within IAI47_01375 at 297.913 kb on - strand, within IAI47_01375 at 297.913 kb on - strand, within IAI47_01375 at 297.913 kb on - strand, within IAI47_01375 at 298.029 kb on + strand, within IAI47_01375 at 298.029 kb on + strand, within IAI47_01375 at 298.030 kb on - strand, within IAI47_01375 at 298.030 kb on - strand, within IAI47_01375 at 298.030 kb on - strand, within IAI47_01375 at 298.042 kb on + strand, within IAI47_01375 at 298.043 kb on - strand, within IAI47_01375 at 298.048 kb on + strand, within IAI47_01375 at 298.049 kb on - strand, within IAI47_01375 at 298.049 kb on - strand, within IAI47_01375 at 298.049 kb on - strand, within IAI47_01375
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 295,108 - IAI47_01365 0.56 +4.0 295,201 + IAI47_01365 0.62 +2.3 295,541 - +1.6 295,541 - +2.5 295,645 - +0.9 295,739 - -0.0 295,752 - -0.1 295,769 + +3.0 295,774 + +3.0 295,857 + +4.3 295,857 + +3.2 295,857 + +2.0 295,857 + +3.7 295,907 + +1.5 295,907 + +1.7 295,912 - +0.8 295,912 - -0.5 295,912 - +4.9 295,912 - -2.5 295,914 + -1.4 295,922 + +0.5 295,927 + +0.2 295,928 - +0.9 295,981 - +1.3 296,001 + -0.3 296,001 + -0.1 296,002 - -1.0 296,034 + +0.6 296,106 - -2.1 296,106 - +0.3 296,182 + IAI47_01370 0.14 +0.0 296,183 - IAI47_01370 0.14 -1.0 296,200 + IAI47_01370 0.15 +0.8 296,201 - IAI47_01370 0.16 +0.6 296,201 - IAI47_01370 0.16 -0.1 296,201 - IAI47_01370 0.16 +2.9 296,201 - IAI47_01370 0.16 -0.8 296,227 - IAI47_01370 0.18 -0.2 296,227 - IAI47_01370 0.18 -0.8 296,245 + IAI47_01370 0.19 -0.3 296,245 + IAI47_01370 0.19 +0.2 296,246 - IAI47_01370 0.19 +0.2 296,246 - IAI47_01370 0.19 -0.0 296,247 + IAI47_01370 0.19 -0.0 296,247 + IAI47_01370 0.19 -0.8 296,247 + IAI47_01370 0.19 -0.3 296,247 + IAI47_01370 0.19 +0.0 296,248 - IAI47_01370 0.19 -0.1 296,263 - IAI47_01370 0.21 +1.0 296,263 - IAI47_01370 0.21 -0.4 296,263 - IAI47_01370 0.21 +0.5 296,268 + IAI47_01370 0.21 +1.2 296,274 + IAI47_01370 0.22 +1.4 296,437 + IAI47_01370 0.35 -1.3 296,437 + IAI47_01370 0.35 -1.6 296,437 + IAI47_01370 0.35 +2.4 296,438 - IAI47_01370 0.36 -1.7 296,438 - IAI47_01370 0.36 -0.7 296,569 + IAI47_01370 0.47 +0.1 296,569 + IAI47_01370 0.47 -1.4 296,570 - IAI47_01370 0.47 +0.1 296,598 + IAI47_01370 0.49 -1.6 296,598 + IAI47_01370 0.49 -0.0 296,598 + IAI47_01370 0.49 +0.3 296,599 - IAI47_01370 0.49 -1.2 296,607 + IAI47_01370 0.50 -1.5 296,607 + IAI47_01370 0.50 +1.3 296,607 + IAI47_01370 0.50 +0.2 296,607 + IAI47_01370 0.50 +0.5 296,607 + IAI47_01370 0.50 -0.9 296,607 + IAI47_01370 0.50 -1.9 296,608 - IAI47_01370 0.50 +0.7 296,608 - IAI47_01370 0.50 -0.4 296,608 - IAI47_01370 0.50 +0.5 296,627 + IAI47_01370 0.51 +0.0 296,627 + IAI47_01370 0.51 -1.0 296,627 + IAI47_01370 0.51 -0.3 296,627 + IAI47_01370 0.51 -1.0 296,627 + IAI47_01370 0.51 +1.2 296,628 - IAI47_01370 0.52 +0.7 296,628 - IAI47_01370 0.52 -1.6 296,628 - IAI47_01370 0.52 -1.6 296,628 - IAI47_01370 0.52 -1.4 296,679 + IAI47_01370 0.56 +0.5 296,688 + IAI47_01370 0.57 -1.1 296,688 + IAI47_01370 0.57 -1.0 296,735 + IAI47_01370 0.61 -1.0 296,752 + IAI47_01370 0.62 -2.3 296,753 - IAI47_01370 0.62 +1.1 296,898 - IAI47_01370 0.74 -0.4 296,898 - IAI47_01370 0.74 -1.3 296,898 - IAI47_01370 0.74 +0.3 296,898 - IAI47_01370 0.74 +2.5 296,898 - IAI47_01370 0.74 -3.8 297,016 + IAI47_01370 0.84 +0.5 297,047 + IAI47_01370 0.87 +0.2 297,048 - IAI47_01370 0.87 +0.6 297,103 + -0.5 297,103 + +1.4 297,103 + +1.1 297,104 - +0.9 297,104 - -0.6 297,104 - +0.3 297,104 - +0.1 297,108 + -0.4 297,109 - -0.3 297,109 - +0.3 297,109 - +0.7 297,109 - -1.0 297,109 - -1.2 297,109 - +0.6 297,148 + -1.5 297,148 + +0.7 297,148 + +1.4 297,148 + -1.3 297,148 + -0.2 297,149 - -0.4 297,149 - +0.5 297,149 - +0.3 297,150 + -0.7 297,150 + +0.9 297,150 + -1.7 297,150 + +0.8 297,150 + +0.2 297,150 + +0.3 297,150 + +0.7 297,150 + +0.7 297,150 + +0.1 297,151 - +0.0 297,151 - +1.3 297,151 - -0.2 297,151 - -0.4 297,151 - +1.0 297,151 - -1.5 297,151 - +0.2 297,151 - -0.5 297,151 - +0.3 297,151 - -1.3 297,151 - -0.2 297,151 - +0.1 297,199 + -0.4 297,199 + -0.2 297,200 - -1.2 297,249 + -0.9 297,249 + -1.9 297,250 - -0.6 297,250 - -0.1 297,274 + +0.5 297,274 + +0.8 297,275 - +0.5 297,462 + IAI47_01375 0.26 +0.1 297,462 + IAI47_01375 0.26 -1.4 297,462 + IAI47_01375 0.26 +0.3 297,462 + IAI47_01375 0.26 +0.5 297,462 + IAI47_01375 0.26 +0.9 297,463 - IAI47_01375 0.26 -1.0 297,463 - IAI47_01375 0.26 -1.6 297,463 - IAI47_01375 0.26 -0.7 297,463 - IAI47_01375 0.26 -0.1 297,463 - IAI47_01375 0.26 -0.0 297,463 - IAI47_01375 0.26 +0.2 297,509 - IAI47_01375 0.30 +0.9 297,509 - IAI47_01375 0.30 -0.7 297,669 + IAI47_01375 0.47 -0.8 297,669 + IAI47_01375 0.47 +2.0 297,748 - IAI47_01375 0.55 +0.3 297,748 - IAI47_01375 0.55 -0.6 297,807 + IAI47_01375 0.61 +0.9 297,807 + IAI47_01375 0.61 -2.0 297,808 - IAI47_01375 0.61 +0.5 297,808 - IAI47_01375 0.61 -0.4 297,808 - IAI47_01375 0.61 +1.4 297,808 - IAI47_01375 0.61 -0.5 297,808 - IAI47_01375 0.61 -1.5 297,808 - IAI47_01375 0.61 -1.6 297,808 - IAI47_01375 0.61 +1.3 297,808 - IAI47_01375 0.61 +0.8 297,810 + IAI47_01375 0.61 +1.9 297,810 + IAI47_01375 0.61 -1.7 297,810 + IAI47_01375 0.61 +0.3 297,810 + IAI47_01375 0.61 -0.4 297,811 - IAI47_01375 0.61 -2.0 297,811 - IAI47_01375 0.61 -2.2 297,811 - IAI47_01375 0.61 -0.6 297,811 - IAI47_01375 0.61 +0.0 297,811 - IAI47_01375 0.61 -0.7 297,811 - IAI47_01375 0.61 +0.1 297,887 - IAI47_01375 0.69 -0.8 297,913 - IAI47_01375 0.71 +0.1 297,913 - IAI47_01375 0.71 +0.5 297,913 - IAI47_01375 0.71 +0.5 298,029 + IAI47_01375 0.83 +0.3 298,029 + IAI47_01375 0.83 -0.5 298,030 - IAI47_01375 0.83 -0.2 298,030 - IAI47_01375 0.83 +2.2 298,030 - IAI47_01375 0.83 -1.0 298,042 + IAI47_01375 0.84 -0.9 298,043 - IAI47_01375 0.84 +1.1 298,048 + IAI47_01375 0.85 -1.0 298,049 - IAI47_01375 0.85 -2.5 298,049 - IAI47_01375 0.85 -0.2 298,049 - IAI47_01375 0.85 -0.3
Or see this region's nucleotide sequence