Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_05805 and IAI47_05810 overlap by 8 nucleotides IAI47_05810 and IAI47_05815 overlap by 4 nucleotides IAI47_05815 and IAI47_05820 overlap by 1 nucleotides IAI47_05820 and IAI47_05825 overlap by 20 nucleotides IAI47_05825 and IAI47_05830 are separated by 150 nucleotides IAI47_05830 and IAI47_05835 overlap by 4 nucleotides
IAI47_05805: IAI47_05805 - DNA replication protein, at 1,203,031 to 1,203,735
_05805
IAI47_05810: IAI47_05810 - hypothetical protein, at 1,203,728 to 1,203,880
_05810
IAI47_05815: IAI47_05815 - hypothetical protein, at 1,203,877 to 1,204,101
_05815
IAI47_05820: IAI47_05820 - DUF3850 domain-containing protein, at 1,204,101 to 1,204,364
_05820
IAI47_05825: IAI47_05825 - hypothetical protein, at 1,204,345 to 1,204,710
_05825
IAI47_05830: IAI47_05830 - hypothetical protein, at 1,204,861 to 1,205,028
_05830
IAI47_05835: IAI47_05835 - hypothetical protein, at 1,205,025 to 1,205,225
_05835
Position (kb)
1203
1204
1205 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1202.891 kb on - strand at 1203.030 kb on + strand at 1203.031 kb on - strand at 1203.031 kb on - strand at 1203.146 kb on + strand, within IAI47_05805 at 1203.176 kb on + strand, within IAI47_05805 at 1203.176 kb on + strand, within IAI47_05805 at 1203.176 kb on + strand, within IAI47_05805 at 1203.176 kb on + strand, within IAI47_05805 at 1203.276 kb on + strand, within IAI47_05805 at 1203.276 kb on + strand, within IAI47_05805 at 1203.276 kb on + strand, within IAI47_05805 at 1203.276 kb on + strand, within IAI47_05805 at 1203.276 kb on + strand, within IAI47_05805 at 1203.276 kb on + strand, within IAI47_05805 at 1203.277 kb on - strand, within IAI47_05805 at 1203.277 kb on - strand, within IAI47_05805 at 1203.277 kb on - strand, within IAI47_05805 at 1203.277 kb on - strand, within IAI47_05805 at 1203.277 kb on - strand, within IAI47_05805 at 1203.412 kb on + strand, within IAI47_05805 at 1203.451 kb on + strand, within IAI47_05805 at 1203.451 kb on + strand, within IAI47_05805 at 1203.451 kb on + strand, within IAI47_05805 at 1203.451 kb on + strand, within IAI47_05805 at 1203.451 kb on + strand, within IAI47_05805 at 1203.451 kb on + strand, within IAI47_05805 at 1203.451 kb on + strand, within IAI47_05805 at 1203.451 kb on + strand, within IAI47_05805 at 1203.451 kb on + strand, within IAI47_05805 at 1203.451 kb on + strand, within IAI47_05805 at 1203.451 kb on + strand, within IAI47_05805 at 1203.451 kb on + strand, within IAI47_05805 at 1203.452 kb on - strand, within IAI47_05805 at 1203.452 kb on - strand, within IAI47_05805 at 1203.452 kb on - strand, within IAI47_05805 at 1203.452 kb on - strand, within IAI47_05805 at 1203.452 kb on - strand, within IAI47_05805 at 1203.452 kb on - strand, within IAI47_05805 at 1203.452 kb on - strand, within IAI47_05805 at 1203.452 kb on - strand, within IAI47_05805 at 1203.452 kb on - strand, within IAI47_05805 at 1203.747 kb on + strand, within IAI47_05810 at 1203.748 kb on - strand, within IAI47_05810 at 1203.765 kb on - strand, within IAI47_05810 at 1203.832 kb on - strand, within IAI47_05810 at 1203.847 kb on - strand, within IAI47_05810 at 1203.887 kb on + strand at 1203.888 kb on - strand at 1203.888 kb on - strand at 1203.888 kb on - strand at 1203.888 kb on - strand at 1203.888 kb on - strand at 1203.888 kb on - strand at 1203.888 kb on - strand at 1203.899 kb on + strand at 1203.903 kb on + strand, within IAI47_05815 at 1203.903 kb on + strand, within IAI47_05815 at 1203.903 kb on + strand, within IAI47_05815 at 1203.904 kb on - strand, within IAI47_05815 at 1203.930 kb on + strand, within IAI47_05815 at 1203.931 kb on - strand, within IAI47_05815 at 1203.931 kb on - strand, within IAI47_05815 at 1203.931 kb on - strand, within IAI47_05815 at 1203.931 kb on - strand, within IAI47_05815 at 1204.043 kb on + strand, within IAI47_05815 at 1204.044 kb on - strand, within IAI47_05815 at 1204.198 kb on + strand, within IAI47_05820 at 1204.198 kb on + strand, within IAI47_05820 at 1204.198 kb on + strand, within IAI47_05820 at 1204.199 kb on - strand, within IAI47_05820 at 1204.199 kb on - strand, within IAI47_05820 at 1204.210 kb on + strand, within IAI47_05820 at 1204.210 kb on + strand, within IAI47_05820 at 1204.211 kb on - strand, within IAI47_05820 at 1204.211 kb on - strand, within IAI47_05820 at 1204.211 kb on - strand, within IAI47_05820 at 1204.211 kb on - strand, within IAI47_05820 at 1204.213 kb on + strand, within IAI47_05820 at 1204.213 kb on + strand, within IAI47_05820 at 1204.213 kb on + strand, within IAI47_05820 at 1204.213 kb on + strand, within IAI47_05820 at 1204.213 kb on + strand, within IAI47_05820 at 1204.213 kb on + strand, within IAI47_05820 at 1204.214 kb on - strand, within IAI47_05820 at 1204.214 kb on - strand, within IAI47_05820 at 1204.214 kb on - strand, within IAI47_05820 at 1204.236 kb on + strand, within IAI47_05820 at 1204.236 kb on + strand, within IAI47_05820 at 1204.236 kb on + strand, within IAI47_05820 at 1204.237 kb on - strand, within IAI47_05820 at 1204.237 kb on - strand, within IAI47_05820 at 1204.237 kb on - strand, within IAI47_05820 at 1204.237 kb on - strand, within IAI47_05820 at 1204.237 kb on - strand, within IAI47_05820 at 1204.264 kb on + strand, within IAI47_05820 at 1204.264 kb on + strand, within IAI47_05820 at 1204.264 kb on + strand, within IAI47_05820 at 1204.264 kb on + strand, within IAI47_05820 at 1204.265 kb on - strand, within IAI47_05820 at 1204.265 kb on - strand, within IAI47_05820 at 1204.265 kb on - strand, within IAI47_05820 at 1204.291 kb on - strand, within IAI47_05820 at 1204.291 kb on - strand, within IAI47_05820 at 1204.313 kb on + strand, within IAI47_05820 at 1204.313 kb on + strand, within IAI47_05820 at 1204.313 kb on + strand, within IAI47_05820 at 1204.313 kb on + strand, within IAI47_05820 at 1204.313 kb on + strand, within IAI47_05820 at 1204.313 kb on + strand, within IAI47_05820 at 1204.313 kb on + strand, within IAI47_05820 at 1204.313 kb on + strand, within IAI47_05820 at 1204.314 kb on - strand, within IAI47_05820 at 1204.314 kb on - strand, within IAI47_05820 at 1204.314 kb on - strand, within IAI47_05820 at 1204.314 kb on - strand, within IAI47_05820 at 1204.314 kb on - strand, within IAI47_05820 at 1204.314 kb on - strand, within IAI47_05820 at 1204.314 kb on - strand, within IAI47_05820 at 1204.403 kb on + strand, within IAI47_05825 at 1204.403 kb on + strand, within IAI47_05825 at 1204.404 kb on - strand, within IAI47_05825 at 1204.404 kb on - strand, within IAI47_05825 at 1204.404 kb on - strand, within IAI47_05825 at 1204.404 kb on - strand, within IAI47_05825 at 1204.418 kb on + strand, within IAI47_05825 at 1204.470 kb on - strand, within IAI47_05825 at 1204.583 kb on + strand, within IAI47_05825 at 1204.583 kb on + strand, within IAI47_05825 at 1204.583 kb on + strand, within IAI47_05825 at 1204.614 kb on + strand, within IAI47_05825 at 1204.618 kb on + strand, within IAI47_05825 at 1204.619 kb on - strand, within IAI47_05825 at 1204.619 kb on - strand, within IAI47_05825 at 1204.711 kb on + strand at 1204.711 kb on + strand at 1204.711 kb on + strand at 1204.711 kb on + strand at 1204.712 kb on - strand at 1204.712 kb on - strand at 1204.712 kb on - strand at 1204.712 kb on - strand at 1204.712 kb on - strand at 1204.712 kb on - strand at 1204.719 kb on + strand at 1204.737 kb on - strand at 1204.737 kb on - strand at 1204.797 kb on + strand at 1204.797 kb on + strand at 1204.798 kb on - strand at 1204.798 kb on - strand at 1204.798 kb on - strand at 1204.798 kb on - strand at 1204.798 kb on - strand at 1204.839 kb on + strand at 1204.839 kb on + strand at 1204.839 kb on + strand at 1204.840 kb on - strand at 1204.840 kb on - strand at 1204.840 kb on - strand at 1204.840 kb on - strand at 1204.843 kb on + strand at 1204.843 kb on + strand at 1204.843 kb on + strand at 1204.843 kb on + strand at 1204.843 kb on + strand at 1204.844 kb on - strand at 1204.844 kb on - strand at 1204.844 kb on - strand at 1204.844 kb on - strand at 1204.844 kb on - strand at 1204.948 kb on + strand, within IAI47_05830 at 1204.948 kb on + strand, within IAI47_05830 at 1204.963 kb on - strand, within IAI47_05830 at 1204.963 kb on - strand, within IAI47_05830 at 1205.035 kb on + strand at 1205.035 kb on + strand at 1205.035 kb on + strand at 1205.035 kb on + strand at 1205.035 kb on + strand at 1205.036 kb on - strand at 1205.036 kb on - strand at 1205.036 kb on - strand at 1205.058 kb on + strand, within IAI47_05835 at 1205.058 kb on + strand, within IAI47_05835 at 1205.058 kb on + strand, within IAI47_05835 at 1205.058 kb on + strand, within IAI47_05835 at 1205.058 kb on + strand, within IAI47_05835 at 1205.058 kb on + strand, within IAI47_05835 at 1205.058 kb on + strand, within IAI47_05835 at 1205.058 kb on + strand, within IAI47_05835 at 1205.058 kb on + strand, within IAI47_05835 at 1205.058 kb on + strand, within IAI47_05835 at 1205.059 kb on - strand, within IAI47_05835 at 1205.059 kb on - strand, within IAI47_05835 at 1205.059 kb on - strand, within IAI47_05835 at 1205.059 kb on - strand, within IAI47_05835 at 1205.059 kb on - strand, within IAI47_05835
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,202,891 - -0.6 1,203,030 + -1.2 1,203,031 - +0.7 1,203,031 - +1.6 1,203,146 + IAI47_05805 0.16 -1.7 1,203,176 + IAI47_05805 0.21 -1.4 1,203,176 + IAI47_05805 0.21 -0.6 1,203,176 + IAI47_05805 0.21 +0.3 1,203,176 + IAI47_05805 0.21 -1.5 1,203,276 + IAI47_05805 0.35 +0.4 1,203,276 + IAI47_05805 0.35 +0.0 1,203,276 + IAI47_05805 0.35 +0.8 1,203,276 + IAI47_05805 0.35 -0.7 1,203,276 + IAI47_05805 0.35 -1.4 1,203,276 + IAI47_05805 0.35 -1.4 1,203,277 - IAI47_05805 0.35 -0.2 1,203,277 - IAI47_05805 0.35 -0.0 1,203,277 - IAI47_05805 0.35 +2.5 1,203,277 - IAI47_05805 0.35 -0.5 1,203,277 - IAI47_05805 0.35 -1.4 1,203,412 + IAI47_05805 0.54 +0.1 1,203,451 + IAI47_05805 0.60 +0.3 1,203,451 + IAI47_05805 0.60 +1.5 1,203,451 + IAI47_05805 0.60 -0.6 1,203,451 + IAI47_05805 0.60 +1.2 1,203,451 + IAI47_05805 0.60 -0.6 1,203,451 + IAI47_05805 0.60 +0.8 1,203,451 + IAI47_05805 0.60 -0.3 1,203,451 + IAI47_05805 0.60 -3.0 1,203,451 + IAI47_05805 0.60 +2.1 1,203,451 + IAI47_05805 0.60 +0.8 1,203,451 + IAI47_05805 0.60 -1.0 1,203,451 + IAI47_05805 0.60 -1.4 1,203,452 - IAI47_05805 0.60 -0.9 1,203,452 - IAI47_05805 0.60 +0.3 1,203,452 - IAI47_05805 0.60 -0.8 1,203,452 - IAI47_05805 0.60 +1.3 1,203,452 - IAI47_05805 0.60 -1.6 1,203,452 - IAI47_05805 0.60 -1.8 1,203,452 - IAI47_05805 0.60 -0.3 1,203,452 - IAI47_05805 0.60 -0.3 1,203,452 - IAI47_05805 0.60 -1.3 1,203,747 + IAI47_05810 0.12 +0.3 1,203,748 - IAI47_05810 0.13 +1.7 1,203,765 - IAI47_05810 0.24 +0.3 1,203,832 - IAI47_05810 0.68 +1.6 1,203,847 - IAI47_05810 0.78 -0.9 1,203,887 + +0.8 1,203,888 - -0.5 1,203,888 - +1.1 1,203,888 - +1.3 1,203,888 - +2.9 1,203,888 - -1.2 1,203,888 - +1.1 1,203,888 - -1.1 1,203,899 + +0.5 1,203,903 + IAI47_05815 0.12 +1.2 1,203,903 + IAI47_05815 0.12 -2.0 1,203,903 + IAI47_05815 0.12 -2.3 1,203,904 - IAI47_05815 0.12 -1.3 1,203,930 + IAI47_05815 0.24 -2.5 1,203,931 - IAI47_05815 0.24 +1.6 1,203,931 - IAI47_05815 0.24 +0.7 1,203,931 - IAI47_05815 0.24 +0.7 1,203,931 - IAI47_05815 0.24 -1.1 1,204,043 + IAI47_05815 0.74 -2.8 1,204,044 - IAI47_05815 0.74 -0.3 1,204,198 + IAI47_05820 0.37 +2.4 1,204,198 + IAI47_05820 0.37 +1.3 1,204,198 + IAI47_05820 0.37 +1.0 1,204,199 - IAI47_05820 0.37 +0.1 1,204,199 - IAI47_05820 0.37 -2.1 1,204,210 + IAI47_05820 0.41 +0.3 1,204,210 + IAI47_05820 0.41 -0.2 1,204,211 - IAI47_05820 0.42 +2.8 1,204,211 - IAI47_05820 0.42 +0.0 1,204,211 - IAI47_05820 0.42 +1.4 1,204,211 - IAI47_05820 0.42 -2.0 1,204,213 + IAI47_05820 0.42 -2.4 1,204,213 + IAI47_05820 0.42 -1.4 1,204,213 + IAI47_05820 0.42 -0.1 1,204,213 + IAI47_05820 0.42 +3.6 1,204,213 + IAI47_05820 0.42 -1.1 1,204,213 + IAI47_05820 0.42 +0.1 1,204,214 - IAI47_05820 0.43 +3.5 1,204,214 - IAI47_05820 0.43 +0.2 1,204,214 - IAI47_05820 0.43 -2.0 1,204,236 + IAI47_05820 0.51 +0.7 1,204,236 + IAI47_05820 0.51 +0.8 1,204,236 + IAI47_05820 0.51 +0.5 1,204,237 - IAI47_05820 0.52 -0.9 1,204,237 - IAI47_05820 0.52 +1.1 1,204,237 - IAI47_05820 0.52 +3.0 1,204,237 - IAI47_05820 0.52 +1.0 1,204,237 - IAI47_05820 0.52 +1.4 1,204,264 + IAI47_05820 0.62 +3.4 1,204,264 + IAI47_05820 0.62 -1.2 1,204,264 + IAI47_05820 0.62 -2.0 1,204,264 + IAI47_05820 0.62 -0.8 1,204,265 - IAI47_05820 0.62 +0.0 1,204,265 - IAI47_05820 0.62 -0.6 1,204,265 - IAI47_05820 0.62 +0.3 1,204,291 - IAI47_05820 0.72 -0.6 1,204,291 - IAI47_05820 0.72 +2.3 1,204,313 + IAI47_05820 0.80 -1.4 1,204,313 + IAI47_05820 0.80 -0.3 1,204,313 + IAI47_05820 0.80 -0.7 1,204,313 + IAI47_05820 0.80 -1.4 1,204,313 + IAI47_05820 0.80 -0.0 1,204,313 + IAI47_05820 0.80 -1.1 1,204,313 + IAI47_05820 0.80 -1.2 1,204,313 + IAI47_05820 0.80 +0.0 1,204,314 - IAI47_05820 0.81 -0.8 1,204,314 - IAI47_05820 0.81 +1.8 1,204,314 - IAI47_05820 0.81 +4.5 1,204,314 - IAI47_05820 0.81 -0.4 1,204,314 - IAI47_05820 0.81 +0.4 1,204,314 - IAI47_05820 0.81 -0.6 1,204,314 - IAI47_05820 0.81 +0.4 1,204,403 + IAI47_05825 0.16 +0.3 1,204,403 + IAI47_05825 0.16 -1.0 1,204,404 - IAI47_05825 0.16 -0.5 1,204,404 - IAI47_05825 0.16 +0.6 1,204,404 - IAI47_05825 0.16 +0.5 1,204,404 - IAI47_05825 0.16 -0.8 1,204,418 + IAI47_05825 0.20 +1.3 1,204,470 - IAI47_05825 0.34 -0.6 1,204,583 + IAI47_05825 0.65 -3.5 1,204,583 + IAI47_05825 0.65 +0.6 1,204,583 + IAI47_05825 0.65 +0.1 1,204,614 + IAI47_05825 0.73 +0.4 1,204,618 + IAI47_05825 0.75 -1.4 1,204,619 - IAI47_05825 0.75 -1.1 1,204,619 - IAI47_05825 0.75 +0.7 1,204,711 + -0.1 1,204,711 + -0.6 1,204,711 + +2.5 1,204,711 + -2.0 1,204,712 - -1.9 1,204,712 - -1.1 1,204,712 - -0.2 1,204,712 - -0.3 1,204,712 - -0.9 1,204,712 - -1.5 1,204,719 + -0.6 1,204,737 - -0.2 1,204,737 - -1.1 1,204,797 + +0.2 1,204,797 + -0.9 1,204,798 - -2.0 1,204,798 - -0.1 1,204,798 - +0.1 1,204,798 - +0.5 1,204,798 - -0.1 1,204,839 + +0.5 1,204,839 + -1.4 1,204,839 + -0.4 1,204,840 - +1.7 1,204,840 - +1.4 1,204,840 - +0.3 1,204,840 - +0.1 1,204,843 + +0.9 1,204,843 + -0.6 1,204,843 + +0.2 1,204,843 + +2.7 1,204,843 + +0.7 1,204,844 - -0.9 1,204,844 - -0.5 1,204,844 - +0.2 1,204,844 - +2.5 1,204,844 - -1.2 1,204,948 + IAI47_05830 0.52 -0.2 1,204,948 + IAI47_05830 0.52 +0.2 1,204,963 - IAI47_05830 0.61 +2.7 1,204,963 - IAI47_05830 0.61 +0.9 1,205,035 + -2.1 1,205,035 + +0.1 1,205,035 + -0.1 1,205,035 + +0.9 1,205,035 + -0.4 1,205,036 - -0.1 1,205,036 - -0.8 1,205,036 - +1.9 1,205,058 + IAI47_05835 0.16 +0.2 1,205,058 + IAI47_05835 0.16 -0.9 1,205,058 + IAI47_05835 0.16 -3.1 1,205,058 + IAI47_05835 0.16 +3.3 1,205,058 + IAI47_05835 0.16 +1.0 1,205,058 + IAI47_05835 0.16 +1.4 1,205,058 + IAI47_05835 0.16 +0.7 1,205,058 + IAI47_05835 0.16 -0.7 1,205,058 + IAI47_05835 0.16 +0.7 1,205,058 + IAI47_05835 0.16 -1.7 1,205,059 - IAI47_05835 0.17 -2.3 1,205,059 - IAI47_05835 0.17 +0.8 1,205,059 - IAI47_05835 0.17 -1.2 1,205,059 - IAI47_05835 0.17 -2.4 1,205,059 - IAI47_05835 0.17 -2.1
Or see this region's nucleotide sequence