Experiment: m.b. Choline Lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c02880 and PGA1_c02890 are separated by 196 nucleotides PGA1_c02890 and PGA1_c02900 overlap by 1 nucleotides PGA1_c02900 and PGA1_c02910 are separated by 3 nucleotides PGA1_c02910 and PGA1_c02920 are separated by 15 nucleotides PGA1_c02920 and PGA1_c02930 overlap by 4 nucleotides
PGA1_c02880: PGA1_c02880 - uvrABC system protein C, at 277,737 to 279,626
_c02880
PGA1_c02890: PGA1_c02890 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase PgsA, at 279,823 to 280,494
_c02890
PGA1_c02900: PGA1_c02900 - putative molybdopterin-converting factor subunit 1, at 280,494 to 280,739
_c02900
PGA1_c02910: PGA1_c02910 - molybdopterin-converting factor subunit 2, at 280,743 to 281,204
_c02910
PGA1_c02920: PGA1_c02920 - hypothetical protein, at 281,220 to 281,534
_c02920
PGA1_c02930: PGA1_c02930 - OmpA domain-containing protein, at 281,531 to 283,441
_c02930
Position (kb) 280
281 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 279.539 kb on + strand at 279.620 kb on + strand at 279.622 kb on + strand at 279.665 kb on + strand at 279.691 kb on - strand at 279.693 kb on + strand at 279.725 kb on - strand at 279.750 kb on + strand at 279.750 kb on + strand at 279.750 kb on + strand at 279.750 kb on + strand at 279.750 kb on + strand at 279.758 kb on - strand at 279.758 kb on - strand at 279.758 kb on - strand at 279.758 kb on - strand at 280.610 kb on + strand, within PGA1_c02900 at 280.618 kb on - strand, within PGA1_c02900 at 280.776 kb on - strand at 280.776 kb on - strand at 280.839 kb on + strand, within PGA1_c02910 at 280.847 kb on - strand, within PGA1_c02910 at 280.847 kb on - strand, within PGA1_c02910 at 280.913 kb on + strand, within PGA1_c02910 at 281.024 kb on + strand, within PGA1_c02910 at 281.056 kb on + strand, within PGA1_c02910 at 281.056 kb on + strand, within PGA1_c02910 at 281.251 kb on - strand at 281.251 kb on - strand at 281.299 kb on + strand, within PGA1_c02920 at 281.322 kb on + strand, within PGA1_c02920 at 281.340 kb on - strand, within PGA1_c02920 at 281.385 kb on + strand, within PGA1_c02920 at 281.390 kb on + strand, within PGA1_c02920 at 281.411 kb on + strand, within PGA1_c02920 at 281.522 kb on - strand at 281.524 kb on + strand at 281.527 kb on - strand at 281.536 kb on + strand at 281.548 kb on + strand at 281.566 kb on - strand at 281.725 kb on - strand, within PGA1_c02930
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Choline Lysine 10 mM remove 279,539 + +2.8 279,620 + -0.4 279,622 + -0.2 279,665 + -0.5 279,691 - +0.9 279,693 + -0.0 279,725 - +0.2 279,750 + -0.7 279,750 + -0.7 279,750 + -1.1 279,750 + -0.1 279,750 + -2.8 279,758 - +0.5 279,758 - -1.3 279,758 - -1.8 279,758 - -0.3 280,610 + PGA1_c02900 0.47 +1.2 280,618 - PGA1_c02900 0.50 -0.0 280,776 - +1.1 280,776 - +0.3 280,839 + PGA1_c02910 0.21 +0.0 280,847 - PGA1_c02910 0.23 -0.8 280,847 - PGA1_c02910 0.23 -0.3 280,913 + PGA1_c02910 0.37 +0.7 281,024 + PGA1_c02910 0.61 -0.7 281,056 + PGA1_c02910 0.68 +0.8 281,056 + PGA1_c02910 0.68 -0.8 281,251 - -0.2 281,251 - +0.2 281,299 + PGA1_c02920 0.25 -1.3 281,322 + PGA1_c02920 0.32 +0.1 281,340 - PGA1_c02920 0.38 -2.3 281,385 + PGA1_c02920 0.52 -2.3 281,390 + PGA1_c02920 0.54 -2.7 281,411 + PGA1_c02920 0.61 -1.5 281,522 - -0.0 281,524 + -3.0 281,527 - -1.1 281,536 + -3.2 281,548 + -1.0 281,566 - +2.3 281,725 - PGA1_c02930 0.10 -1.4
Or see this region's nucleotide sequence