Experiment: m.b. 1-ethyl-3-methylimidazolium chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09880 and PGA1_c09890 are separated by 121 nucleotides PGA1_c09890 and PGA1_c09900 are separated by 235 nucleotides PGA1_c09900 and PGA1_c09910 are separated by 75 nucleotides PGA1_c09910 and PGA1_c09920 are separated by 220 nucleotides
PGA1_c09880: PGA1_c09880 - aminopeptidase N, at 1,020,112 to 1,022,676
_c09880
PGA1_c09890: PGA1_c09890 - hypothetical protein, at 1,022,798 to 1,022,998
_c09890
PGA1_c09900: PGA1_c09900 - malate synthase GlcB, at 1,023,234 to 1,025,366
_c09900
PGA1_c09910: PGA1_c09910 - Uncharacterized protein, possibly involved in utilization of glycolate and propanediol, at 1,025,442 to 1,025,867
_c09910
PGA1_c09920: PGA1_c09920 - putative gamma-glutamyl-gamma-aminobutyrate hydrolase, at 1,026,088 to 1,026,867
_c09920
Position (kb)
1023
1024
1025
1026 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1022.242 kb on + strand, within PGA1_c09880 at 1022.253 kb on + strand, within PGA1_c09880 at 1022.297 kb on + strand, within PGA1_c09880 at 1022.317 kb on - strand, within PGA1_c09880 at 1022.389 kb on + strand, within PGA1_c09880 at 1022.451 kb on + strand at 1022.456 kb on + strand at 1022.519 kb on + strand at 1022.553 kb on + strand at 1022.688 kb on + strand at 1022.688 kb on + strand at 1022.725 kb on + strand at 1022.807 kb on - strand at 1022.946 kb on - strand, within PGA1_c09890 at 1022.985 kb on - strand at 1023.048 kb on - strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.087 kb on + strand at 1023.095 kb on + strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.095 kb on - strand at 1023.236 kb on - strand at 1023.303 kb on + strand at 1023.316 kb on - strand at 1023.345 kb on + strand at 1023.353 kb on - strand at 1023.359 kb on + strand at 1023.359 kb on + strand at 1023.359 kb on + strand at 1023.458 kb on - strand, within PGA1_c09900 at 1023.638 kb on + strand, within PGA1_c09900 at 1023.659 kb on + strand, within PGA1_c09900 at 1023.659 kb on + strand, within PGA1_c09900 at 1023.707 kb on - strand, within PGA1_c09900 at 1023.770 kb on + strand, within PGA1_c09900 at 1023.770 kb on + strand, within PGA1_c09900 at 1023.801 kb on - strand, within PGA1_c09900 at 1024.054 kb on + strand, within PGA1_c09900 at 1024.055 kb on + strand, within PGA1_c09900 at 1024.075 kb on + strand, within PGA1_c09900 at 1024.075 kb on + strand, within PGA1_c09900 at 1024.083 kb on - strand, within PGA1_c09900 at 1024.083 kb on - strand, within PGA1_c09900 at 1024.112 kb on + strand, within PGA1_c09900 at 1024.120 kb on - strand, within PGA1_c09900 at 1024.128 kb on + strand, within PGA1_c09900 at 1024.135 kb on + strand, within PGA1_c09900 at 1024.143 kb on - strand, within PGA1_c09900 at 1024.338 kb on + strand, within PGA1_c09900 at 1024.395 kb on + strand, within PGA1_c09900 at 1024.396 kb on - strand, within PGA1_c09900 at 1024.401 kb on - strand, within PGA1_c09900 at 1024.441 kb on - strand, within PGA1_c09900 at 1024.575 kb on - strand, within PGA1_c09900 at 1024.598 kb on - strand, within PGA1_c09900 at 1024.691 kb on + strand, within PGA1_c09900 at 1024.738 kb on - strand, within PGA1_c09900 at 1024.812 kb on - strand, within PGA1_c09900 at 1024.875 kb on + strand, within PGA1_c09900 at 1024.875 kb on + strand, within PGA1_c09900 at 1024.883 kb on - strand, within PGA1_c09900 at 1024.883 kb on - strand, within PGA1_c09900 at 1025.009 kb on + strand, within PGA1_c09900 at 1025.026 kb on + strand, within PGA1_c09900 at 1025.031 kb on + strand, within PGA1_c09900 at 1025.123 kb on + strand, within PGA1_c09900 at 1025.162 kb on + strand at 1025.193 kb on + strand at 1025.193 kb on + strand at 1025.280 kb on - strand at 1025.293 kb on + strand at 1025.301 kb on - strand at 1025.311 kb on + strand at 1025.341 kb on + strand at 1025.361 kb on + strand at 1025.393 kb on + strand at 1025.452 kb on + strand at 1025.578 kb on + strand, within PGA1_c09910 at 1025.586 kb on - strand, within PGA1_c09910 at 1025.591 kb on - strand, within PGA1_c09910 at 1025.591 kb on - strand, within PGA1_c09910 at 1025.623 kb on - strand, within PGA1_c09910 at 1025.623 kb on - strand, within PGA1_c09910 at 1025.728 kb on - strand, within PGA1_c09910 at 1025.808 kb on - strand, within PGA1_c09910 at 1025.984 kb on + strand at 1026.037 kb on + strand at 1026.053 kb on + strand at 1026.096 kb on + strand at 1026.119 kb on + strand at 1026.129 kb on + strand at 1026.142 kb on + strand at 1026.171 kb on + strand, within PGA1_c09920 at 1026.173 kb on + strand, within PGA1_c09920 at 1026.190 kb on + strand, within PGA1_c09920 at 1026.198 kb on + strand, within PGA1_c09920 at 1026.216 kb on - strand, within PGA1_c09920 at 1026.218 kb on + strand, within PGA1_c09920 at 1026.241 kb on + strand, within PGA1_c09920 at 1026.241 kb on + strand, within PGA1_c09920 at 1026.249 kb on - strand, within PGA1_c09920 at 1026.249 kb on - strand, within PGA1_c09920
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium chloride 5 mM remove 1,022,242 + PGA1_c09880 0.83 -2.2 1,022,253 + PGA1_c09880 0.83 +0.3 1,022,297 + PGA1_c09880 0.85 +0.5 1,022,317 - PGA1_c09880 0.86 -0.9 1,022,389 + PGA1_c09880 0.89 -0.0 1,022,451 + +0.9 1,022,456 + -0.8 1,022,519 + +0.9 1,022,553 + -0.2 1,022,688 + +0.5 1,022,688 + +0.7 1,022,725 + -2.2 1,022,807 - +0.1 1,022,946 - PGA1_c09890 0.74 +0.6 1,022,985 - -1.0 1,023,048 - +1.5 1,023,087 + +1.7 1,023,087 + +0.0 1,023,087 + +0.9 1,023,087 + -0.2 1,023,087 + +0.7 1,023,087 + +1.6 1,023,087 + +0.5 1,023,087 + -0.8 1,023,087 + +1.5 1,023,087 + -0.6 1,023,087 + +0.2 1,023,087 + +1.1 1,023,087 + +0.1 1,023,087 + +1.4 1,023,087 + +0.2 1,023,087 + -0.3 1,023,087 + +0.7 1,023,087 + -0.5 1,023,087 + +0.6 1,023,087 + +1.8 1,023,087 + -0.1 1,023,087 + +0.5 1,023,087 + +0.4 1,023,095 + -0.4 1,023,095 - +0.5 1,023,095 - +0.5 1,023,095 - +0.0 1,023,095 - +0.7 1,023,095 - +1.3 1,023,095 - +0.3 1,023,095 - +0.8 1,023,095 - +0.8 1,023,095 - -0.2 1,023,095 - -0.6 1,023,095 - +0.2 1,023,095 - +0.2 1,023,095 - +0.8 1,023,095 - +0.1 1,023,095 - +2.5 1,023,095 - +0.4 1,023,095 - +0.1 1,023,095 - -2.6 1,023,236 - +1.5 1,023,303 + -0.2 1,023,316 - +0.3 1,023,345 + -1.2 1,023,353 - +2.1 1,023,359 + +2.2 1,023,359 + +0.9 1,023,359 + +1.4 1,023,458 - PGA1_c09900 0.11 +1.5 1,023,638 + PGA1_c09900 0.19 +1.4 1,023,659 + PGA1_c09900 0.20 +1.4 1,023,659 + PGA1_c09900 0.20 +0.6 1,023,707 - PGA1_c09900 0.22 -1.2 1,023,770 + PGA1_c09900 0.25 +1.5 1,023,770 + PGA1_c09900 0.25 +0.3 1,023,801 - PGA1_c09900 0.27 -1.5 1,024,054 + PGA1_c09900 0.38 +1.1 1,024,055 + PGA1_c09900 0.38 +1.2 1,024,075 + PGA1_c09900 0.39 -0.1 1,024,075 + PGA1_c09900 0.39 +2.2 1,024,083 - PGA1_c09900 0.40 +0.8 1,024,083 - PGA1_c09900 0.40 +1.4 1,024,112 + PGA1_c09900 0.41 +0.3 1,024,120 - PGA1_c09900 0.42 +1.1 1,024,128 + PGA1_c09900 0.42 +1.2 1,024,135 + PGA1_c09900 0.42 +0.7 1,024,143 - PGA1_c09900 0.43 +1.3 1,024,338 + PGA1_c09900 0.52 +1.1 1,024,395 + PGA1_c09900 0.54 +1.0 1,024,396 - PGA1_c09900 0.54 +2.0 1,024,401 - PGA1_c09900 0.55 +0.7 1,024,441 - PGA1_c09900 0.57 +0.6 1,024,575 - PGA1_c09900 0.63 +1.1 1,024,598 - PGA1_c09900 0.64 +1.8 1,024,691 + PGA1_c09900 0.68 +1.0 1,024,738 - PGA1_c09900 0.71 +1.1 1,024,812 - PGA1_c09900 0.74 +0.4 1,024,875 + PGA1_c09900 0.77 +0.9 1,024,875 + PGA1_c09900 0.77 +1.1 1,024,883 - PGA1_c09900 0.77 +0.2 1,024,883 - PGA1_c09900 0.77 +2.6 1,025,009 + PGA1_c09900 0.83 -0.1 1,025,026 + PGA1_c09900 0.84 +1.3 1,025,031 + PGA1_c09900 0.84 +0.2 1,025,123 + PGA1_c09900 0.89 +0.1 1,025,162 + -1.1 1,025,193 + +1.0 1,025,193 + +1.4 1,025,280 - +1.5 1,025,293 + +0.7 1,025,301 - +1.0 1,025,311 + +1.3 1,025,341 + +0.2 1,025,361 + -0.9 1,025,393 + +0.9 1,025,452 + +0.9 1,025,578 + PGA1_c09910 0.32 -1.3 1,025,586 - PGA1_c09910 0.34 -1.3 1,025,591 - PGA1_c09910 0.35 +0.7 1,025,591 - PGA1_c09910 0.35 -1.4 1,025,623 - PGA1_c09910 0.42 +0.5 1,025,623 - PGA1_c09910 0.42 -1.3 1,025,728 - PGA1_c09910 0.67 -0.0 1,025,808 - PGA1_c09910 0.86 -0.1 1,025,984 + +1.7 1,026,037 + -1.9 1,026,053 + -1.5 1,026,096 + -1.1 1,026,119 + +0.1 1,026,129 + +0.1 1,026,142 + -0.9 1,026,171 + PGA1_c09920 0.11 +0.7 1,026,173 + PGA1_c09920 0.11 +0.8 1,026,190 + PGA1_c09920 0.13 -0.1 1,026,198 + PGA1_c09920 0.14 +0.5 1,026,216 - PGA1_c09920 0.16 -0.2 1,026,218 + PGA1_c09920 0.17 -0.1 1,026,241 + PGA1_c09920 0.20 -0.1 1,026,241 + PGA1_c09920 0.20 +0.5 1,026,249 - PGA1_c09920 0.21 -0.2 1,026,249 - PGA1_c09920 0.21 +0.7
Or see this region's nucleotide sequence