Experiment: m.b. 1-ethyl-3-methylimidazolium chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09390 and PGA1_c09400 are separated by 156 nucleotides PGA1_c09400 and PGA1_c09410 are separated by 235 nucleotides PGA1_c09410 and PGA1_c09420 are separated by 115 nucleotides
PGA1_c09390: PGA1_c09390 - modification methylase NaeI, at 957,663 to 958,637
_c09390
PGA1_c09400: PGA1_c09400 - tRNA-Gly, at 958,794 to 958,868
_c09400
PGA1_c09410: PGA1_c09410 - hypothetical protein, at 959,104 to 959,310
_c09410
PGA1_c09420: PGA1_c09420 - pyruvate carboxylase Pyc, at 959,426 to 962,992
_c09420
Position (kb) 959
960 Strain fitness (log2 ratio) -2
-1
0
1 at 958.716 kb on + strand at 958.716 kb on + strand at 958.716 kb on + strand at 958.724 kb on - strand at 958.906 kb on - strand at 959.112 kb on + strand at 959.120 kb on - strand at 959.120 kb on - strand at 959.120 kb on - strand at 959.120 kb on - strand at 959.168 kb on - strand, within PGA1_c09410 at 959.168 kb on - strand, within PGA1_c09410 at 959.213 kb on + strand, within PGA1_c09410 at 959.294 kb on + strand at 959.304 kb on + strand at 959.304 kb on + strand at 959.312 kb on - strand at 959.312 kb on - strand at 959.402 kb on + strand at 959.429 kb on + strand at 959.487 kb on + strand at 959.487 kb on + strand at 959.487 kb on + strand at 959.495 kb on - strand at 959.495 kb on - strand at 959.495 kb on - strand at 959.600 kb on + strand at 959.607 kb on + strand at 959.610 kb on + strand at 959.645 kb on - strand at 959.658 kb on + strand at 959.666 kb on - strand at 959.669 kb on + strand at 959.684 kb on - strand at 959.748 kb on + strand at 959.756 kb on - strand at 959.833 kb on + strand, within PGA1_c09420 at 959.833 kb on + strand, within PGA1_c09420 at 959.841 kb on - strand, within PGA1_c09420 at 959.905 kb on + strand, within PGA1_c09420 at 959.976 kb on + strand, within PGA1_c09420 at 959.994 kb on - strand, within PGA1_c09420 at 959.994 kb on - strand, within PGA1_c09420 at 960.002 kb on - strand, within PGA1_c09420 at 960.082 kb on + strand, within PGA1_c09420 at 960.169 kb on - strand, within PGA1_c09420 at 960.185 kb on + strand, within PGA1_c09420 at 960.193 kb on - strand, within PGA1_c09420 at 960.193 kb on - strand, within PGA1_c09420 at 960.193 kb on - strand, within PGA1_c09420 at 960.198 kb on - strand, within PGA1_c09420 at 960.218 kb on + strand, within PGA1_c09420 at 960.229 kb on + strand, within PGA1_c09420 at 960.282 kb on + strand, within PGA1_c09420 at 960.282 kb on + strand, within PGA1_c09420 at 960.287 kb on + strand, within PGA1_c09420 at 960.287 kb on + strand, within PGA1_c09420 at 960.287 kb on + strand, within PGA1_c09420 at 960.287 kb on + strand, within PGA1_c09420 at 960.287 kb on + strand, within PGA1_c09420
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium chloride 5 mM remove 958,716 + -0.3 958,716 + -2.2 958,716 + +0.4 958,724 - +0.5 958,906 - -0.6 959,112 + +0.6 959,120 - +1.5 959,120 - +0.0 959,120 - -0.3 959,120 - +1.3 959,168 - PGA1_c09410 0.31 -0.2 959,168 - PGA1_c09410 0.31 -0.4 959,213 + PGA1_c09410 0.53 -1.6 959,294 + +1.3 959,304 + +0.7 959,304 + +0.5 959,312 - -1.3 959,312 - -0.5 959,402 + +0.5 959,429 + +0.1 959,487 + +0.5 959,487 + +1.2 959,487 + -0.7 959,495 - +1.1 959,495 - +0.9 959,495 - -0.4 959,600 + -0.1 959,607 + +1.4 959,610 + +0.1 959,645 - -1.8 959,658 + -0.2 959,666 - +0.2 959,669 + +1.5 959,684 - +0.4 959,748 + -0.7 959,756 - -0.4 959,833 + PGA1_c09420 0.11 +1.1 959,833 + PGA1_c09420 0.11 -0.0 959,841 - PGA1_c09420 0.12 +0.4 959,905 + PGA1_c09420 0.13 +0.1 959,976 + PGA1_c09420 0.15 +0.1 959,994 - PGA1_c09420 0.16 -0.5 959,994 - PGA1_c09420 0.16 -0.8 960,002 - PGA1_c09420 0.16 +0.5 960,082 + PGA1_c09420 0.18 +0.2 960,169 - PGA1_c09420 0.21 +0.8 960,185 + PGA1_c09420 0.21 +0.8 960,193 - PGA1_c09420 0.22 -0.1 960,193 - PGA1_c09420 0.22 +0.6 960,193 - PGA1_c09420 0.22 -1.0 960,198 - PGA1_c09420 0.22 +0.5 960,218 + PGA1_c09420 0.22 +0.3 960,229 + PGA1_c09420 0.23 -0.6 960,282 + PGA1_c09420 0.24 -0.3 960,282 + PGA1_c09420 0.24 -0.7 960,287 + PGA1_c09420 0.24 -1.5 960,287 + PGA1_c09420 0.24 -0.0 960,287 + PGA1_c09420 0.24 +1.2 960,287 + PGA1_c09420 0.24 -0.5 960,287 + PGA1_c09420 0.24 -2.4
Or see this region's nucleotide sequence